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Issue: Vol. 4303 No. 2: 9 Aug. 2017
Type: Article
Published: 2017-08-09
DOI: 10.11646/zootaxa.4303.2.8
Page range: 284–292
Abstract views: 165
PDF downloaded: 4

Genetic and morphological evidence for a new cryptic species of Ectinogonia (Coleoptera: Buprestidae) from central Chile

Instituto de Ecología y Biodiversidad, Facultad de Ciencias, Universidad de Chile
Laboratorio de Ecología de Ambientes Fragmentados, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile
Instituto de Ecología y Biodiversidad, Facultad de Ciencias, Universidad de Chile
Laboratorio de Genética y Evolución, Facultad de Ciencias, Universidad de Chile.
Coleoptera Chrysochroinae Dicercini Ectinogonia cryptica sp. n. genetic distance taxonomy

Abstract

The genus Ectinogonia Spinola, 1837 is a genus mainly found in Chile; it currently contains 17 species. Recent exploration in the Andes Mountain Range of the Bio Bio Region in Chile have resulted in the collection of specimens slightly different morphologically from all previously described species. The aim of this paper is to describe this new species of Ectinogonia using morphological and genetic evidence. To establish differences between species we described the external morphology and compared it to species that are morphologically similar (i.e. E. buqueti Spinola 1837 and E. intermedia Kerremans 1903). We also measured the genetic differences in COI sequences, constructing a distance matrix in which we compared it to species that are morphologically similar (E. buqueti and E. intermedia) and other species found in the same region (E. speciosa oscuripennis Moore 1994). We found that E. cryptica sp. n. differs from E. buqueti (which previously contained E. cryptica sp. n.) in pronotum and elytral patterns. The genetic distance matrix shows that E. cryptica sp. n. differs by 4.6% from all other Ectinogonia species compared, supporting the morphological evidence.

 

References

  1. Bellamy, C.L. (2006) The Systematic Position of Certain South American Buprestid Genera (Coleoptera: Buprestidae). The Coleopterists Bulletin, 60 (2), 192–196.

    https://doi.org/10.1649/909.1

    Bickford, D., Lohman, D.J., Sodhi, N.S., Ng, P.K., Meier, R., Winker, K., Ingram, K.K. & Das, I. (2007) Cryptic species as a window on diversity and conservation. Trends in ecology & evolution, 22 (3), 148–155

    https://doi.org/10.1016/j.tree.2006.11.004

    Clarissa, M.H. & Simmons, L.W. (2003) Genital morphology and fertilization success in the dung beetle Onthophagus taurus: an example of sexually selected male genitalia. Proceedings of the Royal Society B: Biological Sciences, 270 (1514), 447–455.
    https://doi.org/10.1098/rspb.2002.2266

    Cobos, A. (1954) Revisión de las Ectinogonia Spinola sensu strictus Coleoptera, Buprestidae. Revista Chilena de Entomología, 3 (1953), 41–68.

    Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.

    Han, T., Kang, T., Jeong, J., Lee, Y., Chung, H., Park, S., Lee, S., Kim, K. & Park, H. (2012) Pseudocryptic speciation of Chrysochroa fulgidissima (Coleoptera: Buprestidae) with two new species from Korea, China and Vietnam. Zoological Journal of the Linnean Society, 164, 71–98.
    https://doi.org/10.1111/j.1096-3642.2011.00763.x

    Hansen, J.A., Moulton, J.K., Klingeman, W.E., Oliver, J.B., Windham, M.T., Trigiano, R.N. & Reding, M.E. (2015) Molecular Systematics of the Chrysobothris femorata Species Group (Coleoptera: Buprestidae). Annals of the Entomological Society of America, 108 (5), 950–963.
    https://doi.org/10.1093/aesa/sav080

    Hebert, P.D., Cywinska, A. & Ball, S.L. (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society of London B: Biological Sciences, 270 (1512), 313–321.
    https://doi.org/10.1098/rspb.2002.2218

    Hebert, P.D., Ratnasingham, S. & de Waard, J.R. (2003b) Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society of London B: Biological Sciences, 270 (Supplement 1), S96–S99.
    https://doi.org/10.1098/rsbl.2003.0025

    Hebert, P.D., Penton, E.H., Burns, J.M., Janzen, D.H. & Hallwachs, W. (2004) Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences of the United States of America, 101 (41), 14812–14817.
    https://doi.org/10.1073/pnas.0406166101

    Hopwood, P.E., Head, M.L., Jordan, E.J., Carter, M.J., Davey, E., Moore, A.J. & Royle, N.J. (2016) Selection on an antagonistic behavioral trait can drive rapid genital coevolution in the burying beetle, Nicrophorus vespilloides. Evolution, 70 (6), 1180–1188.
    https://doi.org/10.1111/evo.12938

    Jowett, T. (1986) Preparation of nucleic acids. In: Roberts, D.B. (Eds.), Drosophila: a practical approach. IRL Press, Oxford, pp. 275–286.
    https://doi.org/10.1002/dvg.1020080108

    Kerremans, C. (1903) Genera Insectorum, publies par P. Wytsman. Briissel, Fasc 12, 1–338.

    Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.
    https://doi.org/10.1093/molbev/mst197

    Masly, J.P. (2012) 170 Years of “Lock-and-Key”: Genital Morphology and Reproductive Isolation. International Journal of Evolutionary Biology, 2012, 1–10.
    https://doi.org/10.1155/2012/247352

    Moore, T. (1994) Revisión del género Ectinogonia Spinola para Chile (Coleoptera: Buprestidae). Boletín de la Sociedad de Biología de Concepción, 65, 153–166.

    Moore, T. & Vidal, P. (2015) Análisis de las Especies Chilenas. In: Moore, T. & Vidal, P. (Eds.), Los Bupréstidos de Chile. Ediciones Universidad Católica de Chile, Santiago, pp. 27–337.

    Obenberger, J. (1926) Buprestidae I. In: Junk, W. & Schlenkling, S. (Eds.), Coleopterorum Catalogus. W. Junk, Berlin, pp. 1–212.

    Pentinsaari, M., Mutanen, M. & Kaila, L. (2014) Cryptic diversity and signs of mitochondrial introgression in the Agrilus viridis species complex (Coleoptera: Buprestidae). European Journal of Entomology, 111 (4), 475.
    https://doi.org/10.14411/eje.2014.072

    Polihronakis, M. (2006) Morphometric analysis of intraspecific shape variation in male and female genitalia of Phyllophaga hirticula (Coleoptera: Scarabaeidae: Melolonthinae). Annals of the entomological Society of America, 99 (1), 144–150.
    https://doi.org/10.1603/0013-8746(2006)099[0144:MAOISV]2.0.CO;2

    Pons, J., Barraclough, T., Gomez-Zurita, J., Cardoso, A., Duran, A., Hazell, S., Kamoun, S., Sumlin, W. & Vogler, A. (2006) Sequence-based species delimitation for the DNA taxonomy of undescribed insects. Systematic biology, 55 (4), 595–609.
    https://doi.org/10.1080/10635150600852011

    Richards, O.W. (1927) The specific characters of British Humblebees (Hymenoptera). Transactions of the Royal Entomological Society of London, 75 (2), 233–268.
    https://doi.org/10.1111/j.1365-2311.1927.tb00073.x

    Solier (1849) Historia física y política de Chile. Gay Tomo IV. en el Museo de historia natural de Santiago, En casa del autor, Paris, 500 pp.

    Spinola, M. (1837) Lettre addressee a la Société Entomologique de France sur un groupe de Buprestides. Annales de la Société Entomologique de France, 6, 101–122.

    Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic acids research, 22 (22), 4673–4680.
    https://doi.org/10.1093/nar/22.22.4673

    Zehner, R., Amendt, J., Schütt, S., Sauer, J., Krettek, R. & Povolný, D. (2004) Genetic identification of forensically important flesh flies (Diptera: Sarcophagidae). International journal of legal medicine, 118 (4), 245–247.
    https://doi.org/10.1007/s00414-004-0445-4