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Type: Article
Published: 2018-03-01
Page range: 207–220
Abstract views: 99
PDF downloaded: 3

Morphological and molecular characterization of three Helicotylenchus species from Iran

Unit for Environmental Sciences and Management, North-West University, Private Bag X6001, Potchefstroom, South Africa.
National Collection of Nematodes, Biosystematics, Agricultural Research Council, Plant Protection Research Institute, (ARC-PPRI), Private Bag X134, Queenswood 0121, South Africa
Unit for Environmental Sciences and Management, North-West University, Private Bag X6001, Potchefstroom, South Africa.
Nematology Diagnostic Laboratory, Khosrow-Shirin, Fras Province, Iran.
Nematoda 28S rDNA Helicotylenchus Iran morphology phylogeny

Abstract

Three Helicotylenchus species, namely Helicotylenchus digonicus, Helicotylenchus pseudorobustus and Helicotylenchus vulgaris were identified during a nematode survey conducted in Iran. The morphological and molecular characteristics of the species were defined. In addition, phylogenetic analysis of five Iranian Helicotylenchus populations based on 28S rDNA were done and showed the unresolved position of molecularly identified H. pseudorobustus and H. digonicus. However, populations of H. vulgaris and H. digonicus were placed close to each other. Phylogenetic analysis confirmed that molecularly characterized Helicotylenchus species formed a monophyletic clade. A detailed revision of the species is, however, needed. Illustrations and measurements for all species are provided.

 

References

  1. Bae, C.H., Szalanski, A.L. & Robbins, R.T. (2009) Phylogenetic analysis of Hoplolaiminae inferred from combined D2 and D3 expansion segments of 28S rDNA. Journal of Nematology, 41, 28–34.

    Baermann, G. (1917) Eine einfache Methode zur Auffindung von Ankylostomum (Nematoden) Larven in Erdproben. Geneeskunding Tijdschrift voor Nederlandsch-Indië, 57, 131–137.

    Baur, H. & Leuenberger, C. (2011) Analysis of Ratios in Multivariate Morphometry. Systematic Biology, 60 (6), 813–825.
    https://doi.org/10.1093/sysbio/syr061

    Cobb, N.A. (1893) Plant diseases and their remedies. III. Nematode worms found attacking sugar-cane. Agricultural Gazette of New South Wales, 4, 808–833.

    Cobb, N.A. (1913) New nematode genera found inhabiting fresh water and non-brackish soils. Journal of Washington of the Academy of Sciences, 3, 432–444.
    https://doi.org/10.5962/bhl.part.20323

    De Grisse, A.T. (1969) Contribution to the Morphology and the Systematics of the Criconematidae (Taylor, 1936) Thorne, 1949. Faculty of Agricultural Sciences, Gent, Belgium, 35 pp.

    Fortuner, R. (1984) Morphometrical variability in Helicotylenchus Steiner, 1945. 6: Value of the characters used for specific identification. Revue de Nématologie, 7, 245–264.

    Fortuner, R., Maggenti, A.R. & Whittaker, L.M. (1984) Morphometrical variability in Helicotylenchus Steiner, 1945. 4: Study of field populations of H. pseudorobustus and related species. Revue de Nématologie, 7 (2), 121–135.

    Fortuner, R., Merny, G. & Roux, C. (1981) Morphometrical variability in Helicotylenchus Steiner, 1945. 3: Observations on African populations of Helicotylenchus dihystera and considerations on related species. Revue de Nématologie, 4, 235–260.

    Fortuner, R. (1991) The Hoplolaimidae. In: Nickle, W.R. (Ed.), Manual of Agricultural Nematology, Marcel Dekker, Inc., New York, pp. 669–720.

    Golden, M.A. (1956) Taxonomy of the spiral nematodes (Rotylenchus and Helicotylenchus), and the developmental stages and host-parasite relationships of R. buxophilus n.sp. attacking boxwood. University of Maryland, Agricultural Experiment Station Bulletin, 1956 (No. A–85), 1–28.

    Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.

    Hashim, Z. (1982) Description of the male and notes on the female of Helicotylenchus digonicus Perry in Perry, Darling & Thorne, 1959 (Nematoda: Tylenchida) from Jordan. Nematologica, 28, 206–209.
    https://doi.org/10.1163/187529282X00051

    Hashim, Z. (1985) Plant-parasitic nematodes from vineyards in Jordan. Nematologia Mediterranea, 13, 117–118.

    Hassanzadeh, Z., Karegar, A. & Kheiri, A. (2005) Some species of order Tylenchida collected from alfalfa fields in Hamadan province. Iranian Journal of Plant Pathology, 41, 663–686. [in Persian with English abstract]

    Holovachov, O., Boström, S., De Ley, I.T., Robinson, C., Mundo-Ocampo, M. & Nadler, S.A. (2013) Morphology, molecular characterisation and systematic position of the genus Cynura Cobb, 1920 (Nematoda: Plectida). Nematology, 15, 611–627.
    https://doi.org/10.1163/15685411-00002706

    Knobloch, N.A. & Laughlin, C.W. (1973) A collection of plant parasitic nematodes (Nematoda) from Mexico with descriptions of three new species. Nematologica, 19, 205–217.
    https://doi.org/10.1163/187529273X00330

    Marais, M. (1998) Some species of Helicotylenchus Steiner, 1945 from South Africa (Nematoda: Hoplolaimidae). Fundamental and Applied Nematology, 21, 327–352.

    Marais, M. (2001) A monograph of the genus Helicotylenchus Steiner, 1945 (Nemata: Hoplolaimidae). Ph.D. Dissertation, University of Stellenbosch, Stellenbosch, xi + 266 pp.

    Marais, M. & Swart, A. (1999) Plant nematodes in the Lottering Plantation, Eastern Cape Province. South African Forestry Journal, 186, 77–79.
    https://doi.org/10.1080/10295925.1999.9631245

    McCarthy, C. (1997) Chromas. Version 1.41. Griffith University, Brisbane. [software]

    Mohammad Deimi, A. & Mitkowski, N. (2010) Nematodes associated with vineyards throughout Markazi Province (Arak), Iran. Australian Plant Pathology, 39, 571–577.
    https://doi.org/10.1071/AP10044

    Nyaku, S.T., Kantety, R.V., Lawrence, K.S., van Santen, E. & Sharma, G.C. (2013) Canonical discriminant analysis of Rotylenchulus reniformis in Alabama. Nematropica, 43, 171–181.

    Palomares-Rius, J.E., Castillo, P., Liébanas, G., Vovlas, N., Landa, B.B., Navas-Cortes, J.A., & Subbotin, S.A. (2010) Description of Pratylenchus hispaniensis n. sp. from Spain and considerations on the phylogenetic relationship among selected genera in the family Pratylenchidae. Nematology, 12, 429–451.
    https://doi.org/10.1163/138855409X12559479585043

    Perry, V.G., Darling, H.M. & Thorne, G. (1959) Anatomy, taxonomy and control of certain spiral nematodes attacking blue grass in Wisconsin. University of Wisconsin, Agricultural Experiment Station, Research Bulletin, 207, 1–24.

    Rathore Y.S., & Tiwari S.N. (2016) Relationships of Species of Helicotylenchus with Taxonomic Groupings of Host Plants. Journal of Plant Science Research, 3 (1), 145.

    Ronquist, F. & Huelsenbeck, J. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.
    https://doi.org/10.1093/bioinformatics/btg180

    Schreck Reis, C., Vieira Dos Santos, M.C., Marais, M., Santos, M.S.N.A., Duyts, H., Freitas, H., Van Der Putten, W.H. & Abrantes, I.M.D.O. (2010) First record of Helicotylenchus varicaudatus Yuen, 1964 (Nematoda: Hoplolaimidae) parasitizing Ammophila arenaria (L). Links in Portuguese coastal sand dunes. Phytopathologia Mediterranea, 49, 212–226.

    Sher, S.A. (1961) Revision of the Hoplolaiminae (Nematoda) I. Classification of nominal genera and nominal species. Nematologica, 6, 155–169.
    https://doi.org/10.1163/187529261X00414

    Sher, S.A. (1966) Revision of the Hoplolaiminae (Nematoda) VI. Helicotylenchus Steiner, 1945. Nematologica, 12, 1–56.
    https://doi.org/10.1163/187529266X00013

    Steiner, G. (1914) Freilebende Nematodes aus der Schweiz. 1. Teil einer vorläufigen Mitteilung. Archiv für Hydrobiologieund Planktonkunde, 9, 259–276.

    Steiner, G. (1945) Helicotylenchus, a new genus of plant-parasitic nematodes and its relationship to Rotylenchus Filipjev. Proceedings of the Helminthological Society of Washington, 12, 34–38.

    Subbotin, S.A., Inserra, R.N., Marais, M., Mullin, P., Powers, T.O., Roberts, P.A., Van den Berg, E., Yeates, G.W. & Baldwin, J.G. (2011) Diversity and phylogenetic relationships within the spiral nematodes of Helicotylenchus Steiner, 1945 (Tylenchida: Hoplolaimidae) as inferred from analysis of the D2-D3 expansion segments of 28S rRNA gene sequences. Nematology, 13, 333–345.
    https://doi.org/10.1163/138855410X520936

    Subbotin, S.A., Sturhan, D., Chizhov, V.N., Vovlas, N. & Baldwin, J.G. (2006) Phylogenetic analysis of Tylenchida Thorne, 1949 as inferred from D2 and D3 expansion fragments of the 28S rRNA gene sequences. Journal of Nematology, 8, 455–474.
    https://doi.org/10.1163/156854106778493420

    Subbotin, S.A., Vovlas, O., Yeates, G.W., Hallmann, J., Kiewnick, S., Chizhov, V.N., Manzanilla-López, R.H., Inserra, R.N. & Castillo, P. (2015) Morphological and molecular characterisation of Helicotylenchus pseudorobustus (Steiner, 1914) Golden, 1956 and related species (Tylenchida: Hoplolaimidae) with a phylogeny of the genus. Nematology, 17, 27–52.
    https://doi.org/10.1163/15685411-00002850

    Thorne, G. (1935). Nemic parasites and associates of the mountain pine beetle (Dendroctonus monicolae) in Utah. Journal of Agricultural Research U. S. Department of Agriculture, 51, 131–144.

    Venables, W.N. & Ripley, B.D. (2002) Modern Applied Statistics with S. 4th Edition. Springer, New York, 498 pp. [ISBN 0–387–95457–0]
    https://doi.org/10.1007/978-0-387-21706-2

    Wouts, W.M. & Knight, K.W.L. (1993) Helicotylenchus vulgaris Yuen, 1964 (Nematoda: Hoplolaimidae): a new record for New Zealand. New Zealand Journal of Zoology, 20, 133–136.
    https://doi.org/10.1080/03014223.1993.10422855

    Wouts, W.M. & Yeates, G.W. (1994) Helicotylenchus species (Nematoda: Tylenchida) from native vegetation and undisturbed soils in New Zealand. New Zealand Journal of Zoology, 21, 213–224.
    https://doi.org/10.1080/03014223.1994.9517988

    Ye, W.M. & Robbins, R.T. (2004) Stepwise and canonical discriminant analysis of Longidorus species (Nematoda: Longidoridae) from Arkansas. Journal of Nematology, 36, 449------------------–457.

    Yuen, P.H. (1964) Four new species of Helicotylenchus Steiner (Hoplolaiminae: Tylenchida) and a redescription of H. canadensis Waseem, 1961. Nematologica, 10, 373–387.
    https://doi.org/10.1163/187529264X00385