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Type: Article
Published: 2000-12-31
Page range: 73–80
Abstract views: 105
PDF downloaded: 0

Oxidation reactions are required to produce atranorin from acetate by alginate-immobilized cells of Cladonia verticillaris.

Laboratory of Plant Physiology, The Lichen Team. Faculty of Biology, Complutense University, 28040 Madrid, Spain
Laboratory of Plant Physiology, The Lichen Team. Faculty of Biology, Complutense University, 28040 Madrid, Spain
Departamento de Ciências Geográficas, CFCH/ Departamento de Botânica, CCB, Universidade Federal de Pernambuco. Cidade Universitaria, CEP 50.640-901, Recife, PE. Brasil.
Laboratory of Plant Physiology, The Lichen Team. Faculty of Biology, Complutense University, 28040 Madrid, Spain
Bryophyta Cladonia verticillaris

Abstract

Atranorin, a p-depside of the b-orcinol series, is produced by several Cladonia species. Immobilized cells of Cladonia verticillaris in calcium alginate are able to produce atranorin when they are supplied with 1.0 mM acetate as a precursor. Production of the depside is enhanced by adding an oxidant agent (NAD+ or FMN) to the incubation media and its secretion to these media is facilitated by permeabilizing the immobilized cells with 2 % iso-propanol.

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