Abstract
In vitro studies on two Indian Anthocerote taxa viz. Anthoceros bharadwajii Udar et Asthana and Phaeoceros carolinianus (Michx.) Prosk. have been carried out on agar medium by inoculating their spores into a range of inorganic media and their growth and development were studied in vitro. Spore germination of A. bharadwajii was exosporous while that of P. carolinianus was endosporous. Half strength Knop’s macronutrients medium under alternate light of 4000-5000 lux for 14 hours and dark period for 10 hours at the temperature 200C was found the most suitable for growth and multiplication of the species. Addition of sucrose and trace elements inhibited their growth. Temperature above 270C proved fatal even for a short time. To avoid dryness in medium regular sub culturing was found essential for the growth of healthy culture.