Molluscan Research 28(3):
158-164; published 22 Dec. 2008
Copyright © The
Malacological Society of Australasia
mRNA expression of HSP90 and SOD, and
physiological responses to thermal and osmotic stress in the Pacific
oyster, Crassostrea gigas
PIL GUE JO1,
KWANG WOOK AN1, MI SEON PARK2 & CHEOL YOUNG
CHOI1*
1Division
of Marine Environment & Bioscience, Korea Maritime University,
Busan 606-791, Republic of Korea. Email:
choic@hhu.ac.kr (C.Y. Choi)
2Aquaculture
Research Department, National Fisheries Research & Development
Institute, Busan 619-705, Republic of Korea
* Corresponding author
Abstract
We
investigated mRNA expression of heat shock protein 90 (HSP90) and
superoxide dismutase (SOD) in gill tissues, and osmolality and
hydrogen peroxide (H2O2) concentration in the
haemolymph of Pacific oysters, Crassostrea gigas, exposed to
30ºC and 35 psu (thermal stress) and 20ºC and 52.5 psu (osmotic
stress). HSP90 and SOD mRNA expression and H2O2
concentration were highest at 6 h, and osmolality was highest at 24
h during thermal stress experiments at 30ºC. HSP90 mRNA expression
and H2O2 concentration was highest at six
days, SOD mRNA expression was highest at nine days, and osmolality
maximal at three days in
C. gigas exposed to osmotic stress at 52.5 psu. These results
indicate that high water temperature and high salinity induce
changes in osmolality and H2O2 concentration,
and that SOD and HSP90 play important roles in the effects of
temperature and salinity on the gills of oysters. We suggest that
the pattern of HSP90 mRNA expression is related to its function as a
molecular chaperone contributing to the folding and maintenance of
structural integrity, and that SOD mRNA was highly expressed as a
defense against reactive oxygen species.
Key
words: Heat shock proteins, H2O2,
HSP90, osmolality, SOD.
Full article (PDF;
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