Molluscan ResearchISSN 1323-5818
 An international journal of the Malacological Society of Australasia and 
the Society for the Study of Molluscan Diversity published by Magnolia Press

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Molluscan Research 31(3): 141-151; published 30 Nov. 2011
Copyright © The Malacological Society of Australasia & the Society for the Study of Molluscan Diversity

Molecular analysis of adults and egg masses reveals two independent lineages within the infaunal gastropod Naticarius onca (Röding, 1798) (Caenogastropoda: Naticidae)

THOMAS HUELSKEN1*, HEIKE WÄGELE2, BJÖRN PETERS3, ANDREW MATHER1 & MICHAEL HOLLMANN3

1 School of Biological Sciences, The University of Queensland, Qld 4072 Brisbane, Australia

2 Zoologisches Forschungsmuseum Alexander Koenig, Adenauerallee 160, 53113 Bonn

3 Dept. Biochemistry I - Receptor Biochemistry, Ruhr University Bochum, Universitaetsstrasse 150, 44780 Bochum, Germany

*Corresponding author—Email: t.huelsken@gmail.com

Abstract

We analysed sympatrically occurring specimens from Lizard Island, Queensland, Australia identified on shell characters as Naticarius onca (Röding, 1798) and found them molecularly separated into two distinct clades. Additionally, we obtained sequences from nine morphologically similar, randomly collected naticid egg masses (‘sand collars’) from Lizard Island and two other Queensland localities. Eight out of the nine egg masses unambiguously grouped with one of the two N. onca clades. The two clades show no common haplotypes, resulting in a sequence divergence of 11.1% in the COI gene fragment, and 4.0% in the 16S gene fragment, while the intraspecific variability within the two taxa was 1.0–2.4% and 0.2–0.4% for COI and 16S, respectively. The adult shells of members of the two clades from Lizard Island are morphologically indistinguishable, showing overlapping intraspecific shell variability in colour pattern, shape, and protoconch morphology. Thus, N. onca contains at least two independent lineages that probably represent distinct species. One of the egg masses from Lizard Island, which is morphologically similar to N. onca, proved to be assigned to the rare N. concinnus, the first documented evidence of this species from the Great Barrier Reef. Our results illustrate the usefulness of egg masses in barcoding approaches and taxonomic assignments.

Key words: barcoding, protoconch, cryptic species, COI, mitochondrial DNA, Gastropoda, systematics

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