Abstract
The genus Phaeorrhiza is reported as new for the Chinese lichen biota, including the two species, Phaeorrhiza nimbosa (Fr.) H. Mayrhofer & Poelt and P. sareptana var. sphaerocarpa. (Tr. Fr.) H. Mayrhofer & Poelt. The genus grows on soil in alpine meadows, morphologically characterized by a squamulose to subfoliose thallus, rhizohyphae, and Beltraminia-type ascospores. P. nimbosa has cryptolecanorine to eulecanorine apothecia, while P. sareptana var. sphaerocarpa has lecideine apothecia. Descriptions, a key and a phylogram of these species are provided.
References
<p>Bungartz, F., Nordin, A. & Grube, U. (2007) Buellia. In: Nash, III T.H., Gries, C. & Bungartz, F. (Eds.) Lichen flora of the Greater Sonoran Desert region, volume 3. Tempe: Arizona State University, pp. 113–178.<br>Culberson, C.F. (1972) Improved conditions and new data for identification of lichen products by standardized thin-layer chromatographic method. Journal of Chromatography A 72 (1): 113–125. https://doi.org/10.1016/0021-9673(72)80013-X<br>Diederich, P. & Zhurbenko, M.P. (2009) Sphaerellothecium phaeorrhizae and Zwackhiomyces sipmanii spp. nov. on Phaeorrhiza sareptana from north-eastern Asia, with a key to the species of Sphaerellothecium. Bibliotheca Lichenologica 99: 113–122.<br>Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for basidiomycetes-application for the identification of mycorrhizae and rusts. Molecular Ecology 2: 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x<br>Grube, M. & Arup, U. (2001) Molecular and morphological evolution in the Physciaceae (Lecanorales, lichenized Ascomycotina), with special emphasis on the genus Rinodina. Lichenologist 33 (1): 63–72. https://doi.org/10.1006/lich.2000.0297<br>Guindon, S., Dufayard, J.F., Lefort, V., Anisimova, M., Hordijk, W. & Gascuel, O. (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology 59 (3): 307–321. https://doi.org/10.1093/sysbio/syq010<br>Hansen, E.S. (1987) New or interesting Greenland lichens IV. Mycotaxon 30: 69–80.<br>Hansen, E.S. (2000) A contribution to the lichen flora of the Kangerlussuaq area, West Greenland. Cryptogamie Mycologie 21 (1): 53–59. https://doi.org/10.1016/S0181-1584(00)00104-4<br>Harutyunyan, S. & Mayrhofer, H. (2009) A contribution to the lichen mycota of Armenia. Bibliotheca Lichenologica 100: 137–156.<br>Harutyunyan, S., Wiesmayr, B. & Mayrhofer, H. (2011) Catalogue of the lichenized fungi of Armenia. Herzogia 24 (2): 265–296. https://doi.org/10.13158/heia.24.2.2011.265<br>Hassouna, N., Mithot, B. & Bachellerie, J.P. (1984) The complete nucleotide sequence of mouse 28S rRNA gene. Implications for the process of size increase of the large subunit rRNA in higher eukaryotes. Nucleic Acids Research 12: 3563–3583. https://doi.org/10.1093/nar/12.8.3563<br>Helms, G., Friedl, T. & Rambold, G. (2003) Phylogenetic relationships of the Physciaceae inferred from rDNA sequence data and selected phenotypic characters. Mycologia 95 (6): 1078–1099. https://doi.org/10.2307/3761914<br>Hou, X. (2001) Vegetation Altas of China. Science Press, Beijing, China.<br>Huneck, S., Schmidt, J. & Mayrhofer, H. (1989) Zur Chemie der Flechte Phaeorrhiza nimbosa. Herzogia 8: 137–139.<br>John, V. & Türk, A. (2017) Türkiye Likenleri Listesi (A checklist of the lichens of Turkey). İstanbul: Nezahat Gökyiğit Botanik Bahçesi Yayımı.<br>Katoh, K., Misawa, K., Kuma, K. & Miyata, T. (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research 30 (14): 3059–3066. https://doi.org/10.1093/nar/gkf436<br>Kudratov, I. & Mayrhofer, H. (2002) Catalogue of the lichenized and lichenicolous fungi of Tajikistan. Herzogia 15: 91–128.<br>Lanfear, R., Calcott, B., Ho, S.Y. & Guindon S. (2012) PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution 29 (6): 1695–1701. https://doi.org/10.1093/molbev/mss020<br>Lanfear, R., Frandsen, P. B., Wright, A. M., Senfeld, T. & Calcott, B. (2017) PartitionFinder 2: new methods for selecting partitioned models of evolution for molecular and morphological phylogenetic analyses. Molecular Biology and Evolution 34 (3): 772–773. https://doi.org/10.1093/molbev/msw260<br>Lohtander, K., Ahti, T., Stenroos, S. & Urbanavichus, G. (2008) Is Anaptychia monophyletic? A phylogenetic study based on nuclear and mitochondrial genes. Annales Botanici Fennici 45 (1): 55–60. https://doi.org/10.5735/085.045.0106<br>Lohtander, K., Källersjö, M., Moberg, R. & Tehler A. (2000) The family Physciaceae in Fennoscandia: phylogeny inferred from ITS sequences. Mycologia 92 (4): 728–735. https://doi.org/10.1080/00275514.2000.12061212<br>Magnusson, A.H. (1957) Some interesting lichens from Norway. Nytt Magasin for Botanikk 5: 17–21.<br>Marthinsen, G., Rui S. & Timdal, E.O. (2019) A reference library of DNA barcodes for Nordic lichens. Biodiversity Data Journal 7: 1–146. https://doi.org/10.3897/BDJ.7.e36252<br>Mayrhofer, H. & Poelt J. (1978) Phaeorrhiza, eine neue Gattung der Physciaceae (Lichenes). Nova Hedwigia 30: 781–798. https://doi.org/10.1127/nova.hedwigia/30/1979/781<br>Molina, M.C., Divakar, P.K. & González, N. (2015) Success in the isolation and axenic culture of Anaptychia ciliaris (Physciaceae, Lecanoromycetes) mycobiont. Mycoscience 56 (4): 351–358. https://doi.org/10.1016/j.myc.2014.10.003<br>Orange, A., James, P.W. & White, F.J. (2001) Microchemical methods for the identification of lichens. British Lichen Society, London, UK. <br>Rambaut, A. (2012) FigTree v. 1.4.0. Institute of Evolutionary Biology, University of Edinburgh. [http://tree.bio.ed.ac.uk/software/figtree/]<br>Rehner, S.A. & Samuels, G.J. (1994) Taxonomy and phylogeny of Gliocladium analysed from nuclear large subunit ribosomal DNA sequences. Mycological Research 98 (6): 625–634. https://doi.org/10.1016/S0953-7562(09)80409-7<br>Resl, P., Mayrhofer, H., Clayden, S.R., Spribille, T., Thor, G., Tønsberg, T. & Sheard, J.W. (2016) Morphological, chemical and species delimitation analyses provide new taxonomic insights into two groups of Rinodina. Lichenologist 48 (5): 469–488. https://doi.org/10.1017/S0024282916000359<br>Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: Ecient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61 (3): 539–542. https://doi.org/10.1093/sysbio/sys029<br>Sheard, J.W. (2010) The lichen genus Rinodina (Lecanoromycetidae, Physciaceae) in North America, north of Mexico. NRC Research Press, Ottawa, pp. 9–10.<br>Shi, H., Wang, X., Zhou, Y., Liu, D., Zhang, Y., Yang, M., Timdal, E. & Wang. L. (2018) Three new species and one new combination of Gypsoplaca (lichenized Ascomycota) from the Hengduan Mountains in China. Mycological Progress 17: 781–790. https://doi.org/10.1007/s11557-018-1396-3<br>Stamatakis, A. (2014) RAxML Version 8: A tool for Phylogenetic Analysis and Post-Analysis of Large Phylogenies. Bioinformatics 30 (9): 1312–1313. https://doi.org/10.1093/bioinformatics/btu033<br>Trass, H. (1998) List of physcioid macrolichens of Russian Far East and Siberia. Folia Cryptogamica Estonica 33: 147–151.<br>Valadbeigi, T. & Sipman, H.J.M. (2010) New records of lichens and lichenicolous fungi from Iran and their biogeographical significance. Mycotaxon 113 (1): 191–194. https://doi.org/10.5248/113.191<br>Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172 (8): 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990<br>Wagner, V. & Spribille T. (2005) Preliminary checklist of the lichens of Kazakhstan. [https://www.researchgate.net/publication/237216241_Preliminary_checklist_of_the_lichens_of_Kazakhstan]<br>Wang, L.S., Wang, X.Y., Liu, D., Myllys, L., Shi, H.X., Zhang. Y.Y., Yang, M.X. & Li, L.L. (2017) Four new species of Bryoria (Lichenized Ascomycota: Parmeliaceae) from the Hengduan Mountains, China. Phytotaxa 297 (1): 29–41. https://doi.org/10.11646/phytotaxa.297.1.3<br>Wedin, M., Döring, H., Nordin, A. & Tibell, L. (2000) Small subunit rDNA phylogeny shows the lichen families Caliciaceae and Physciaceae (Lecanorales, Ascomycotina) to form a monophyletic group. Canadian Journal of Botany 78: 246–254. https://doi.org/10.1139/b99-185<br>White, T.J., Bruns, T.D., Lee, S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal DNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. Academic Press, San Diego, pp. 315–321. https://doi.org/10.1016/B978-0-12-372180-8.50042-1<br>Zhao, X., Zhang, L.L., Zhao, Z.T., Wang, W.C., Leavitt, S.D. & Lumbsch, T. (2015) Molecular phylogeny of the lichen genus Lecidella focusing on species from mainland China. PloS ONE 10 (9): e0139405. https://doi.org/10.1371/journal.pone.0139405<br>Zhurbenko, M., Enkhtuya, O. & Javkhlan, S. (2020) Additions to the checklist of lichenicolous fungi of Mongolia. Folia Cryptogamica Estonica Fasc. 57: 9–20. https://doi.org/10.12697/fce.2020.57.03<br>Zoller, S., Scheidegger, C. & Sperisen, C. (1999) PCR primers for the amplification of mitochondrial small subunit ribosomal DNA of lichen-forming ascomycetes[J]. Lichenologist 31 (5): 511–516. https://doi.org/10.1017/S0024282999000663</p>