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Article
Published: 2021-09-21

Cortinarius anaunianus (Agaricales, Cortinariaceae): a new species of the Humolentes clade from Italy

Via G. Canestrini 10/B, I-38028, Novella (TN), Italy
Via Murazzano 11, 10141, Torino (TO), Italy
Santo Stefano, 46, 39030, San Lorenzo di Sebato (BZ), Italy
Dipartimento di Scienze della Vita e Biologia dei Sistemi, Viale P. A. Mattioli 25, 10125 Torino, Italy
Agaricomycetes Basidiomycota Calochroid clade molecular phylogeny

Abstract

Cortinarius anaunianus is described as a new species within the Humolentes clade of the section Calochroi s. lato from alpine silver fir forests (Abies alba) in northeastern Italy (Val di Non, province of Trento). Phylogeny based on the combined nrDNA ITS and LSU, rpb1, rpb2 and the coded gaps of the ITS region supports the independence of C. anaunianus from the closest taxa and its sister position to C. humolens from which it differs by (i) more vivid olivaceous tinges on pileus and lamellae when young, (ii) pileus surface strongly turning ocher-brown with age and exposure (iii) growing in silver fir forests rather than in association with broadleaved trees. Photos of fresh basidiomes in field and a full description of its main macro- and micromorphological features are provided and compared to those of the morphologically most similar species.

References

  1. Alvarado, P., Moreno, G. & Manjón, J.L. (2012) Comparison between Tuber gennadii and T. oligospermum lineages reveals the existence of the new species T. cistophilum (Tuberaceae, Pezizales). Mycologia 104 (4): 894–910. https://doi.org/10.3852/11-254

  2. Armada, F. (2018) Quelques cortinaires calochroides et glaucopoides récoltés en France et en Espagne 134: 55–86.

  3. Bidaud, A., Carteret, X., Eyssartier, G., Moënne-Loccoz, P. & Reumaux, P. (2004) Atlas des Cortinaires. Pars XIV. Éd. Fédération mycologique Dauphiné-Savoie. F-Marlioz.

  4. Borchsenius, F. (2009) FastGap 1.2. Department of Biosciences, Aarhus University, Denmark. Available from: http://www.aubot.dk/FastGap_home.htm (accessed 21 September 2021)

  5. Brandrud, T.E. (1998) Cortinarius subgen. Phlegmacium sect. Fulvi chemotaxonomy versus morphological taxonomy Journal des J.E.C: 0.

  6. Brandrud, T.E., Lindström, H., Marklund, H., Melot, J. & Muskos, S. (1989) Cortinarius Flora Photographica I. Cortinarius HB, Matfors.

  7. Brandrud, T.E., Lindström, H., Marklund, H., Melot, J. & Muskos, S. (1992) Cortinarius Flora Photographica II Cortinarius HB, Matfors.

  8. Brandrud, T.E., Lindström, H., Marklund, H., Melot, J. & Muskos, S. (1998) Cortinarius Flora Photographica IV Cortinarius HB, Matfors.

  9. Brandrud, T.E., Lindström, H., Marklund, H., Melot, J. & Muskos, S. (1989-2018) Cortinarius Flora Photographica. I–V. Cortinarius HB, Sweden.

  10. Brandrud, T.E., Schmidt-Stohn, G. & Dima, B. (2019) Cortinarius hildegardiae and C. mariekristinae spp. nov., two new species in the phlegmacioid clade Humolentes (sect. Calochroi s. l.). Sydowia 71: 0115–0127. https://doi.org/10.12905/0380.sydowia71-2019-0115

  11. Consiglio, G., Antonini, D. & Antonini, M. (2005) Il Genere Cortinarius in Italia III. Associazione Micologica Bresadola, Brescia.

  12. Crous, P.W., Cowan, D.A., Maggs-Kölling, G., Yilmaz, N., Larsson, E., Angelini, C., Brandrud, T.E. Dearnaley, J.D.W., Dima, B., Dovana, F., Fechner, N., García, D., Gené, J., Halling, R.E., Houbraken, J., Leonard, P., Luangsa-ard, J.J., Noisripoom, W., Rea-Ireland, A.E., Ševčíková, H., Smyth, C.W., Vizzini, A., Adam, J.D., Adams, G.C., Alexandrova, A.V., Alizadeh, A., Álvarez, Duarte, E., Andjic, V., Antonín, V., Arenas, F., Assabgui, R., Ballarà, J., Banwell, A., Berraf-Tebbal, A., Bhatt, V.K., Bonito, G., Botha, W., Burgess, T.I., Caboň, M., Calvert, J., Carvalhais, L.C., Courtecuisse, R., Cullington, P., Davoodian, N., Decock, C.A., Dimitrov, R., Di Piazza, S., Drenth, A., Dumez, S., Eichmeier, A., Etayo, J., Fernández, I., Fiard, J.P., Fournier, J., Fuentes-Aponte, S., Ghanbary, M.A.T., Ghorbani, G., Giraldo, A., Glushakova, A.M., Gouliamova, D.E., Guarro, J., Halleen, F., Hampe, F., Hernández-Restrepo, M., Iturrieta-González, I., Jeppson, M., Kachalkin, A.V., Karimi, O., Khalid, A.N., Khonsanit, A., Kim, J.I., Kim, M. Kiran, K., Krisai-Greilhuber, I., Kučera, V., Kušan, I., Langenhoven, S.D., Lebel, T., Lebeuf, R., Liimatainen, K., Linde, C., Lindner, D.L., Lombard, L., Mahamedi, A.E., Matočec, N., Maxwell, A., May, T.W., McTaggart, A.R., Meijer, M., Mešić, A., Mileto, A.J., Miller, A.N., Molia, A., Mongkolsamrit, S., Muñoz Cortés, J. Muñoz-Mohedano, C., Morte, A., Morozova, O.V., Mostert, L., Mostowfizadeh-Ghalamfarsa, R., Nagy, L.G., Navarro-Ródenas, A., Örstadius, L., Overton, B.E., Papp, V., Para, R., Peintner, U., Pham, T.H.G., Pordel, A., Pošta, A., Rodríguez, A., Romberg, M., Sandoval-Denis, M., Seifert, K.A., Semwal, K.C., Sewall, B.J., Shivas, R.G., Slovák, M., Smith, K., Spetik, M., Spies, C.F.J., Syme, K., Tasanathai, K., Thorn, R.G., Tkalčec, Z., Tomashevskaya, M.A., Torres-Garcia, D., Ullah, Z., Visagie, C.M., Voitk, A., Winton, L.M. & Groenewald, J.Z. (2020) Fungal Planet description sheets: 1112–1181. Persoonia 45: 251–409. https://doi.org/10.3767/persoonia.2020.45.10

  13. Cubeta, M.A., Echandi, E., Abernethy, T. & Vilgalys, R. (1991) Characterization of anastomosis groups of binucleate Rhizoctonia species using restriction analysis of an amplified ribosomal RNA gene. Phytopathology 81: 1395–1400. https://doi.org/10.1094/Phyto-81-1395

  14. Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods 9 (8): 772. https://doi.org/10.1038/nmeth.2109

  15. Dima, B. & Albert, L. (2011) A Cortinarius humolens Magyarorszàgon-Mikol. Kozelm. Clusiana 50 (2): 141–148.

  16. Eyssartier, G. (2011) Cortinarius lavandulochlorus sp. nov., un nouveau cortinaire proche de C. olivellus Rob. Henry. Journal des J.E.C. 13: 52–57.

  17. Felsenstein, J. (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39 (4): 783–791. https://doi.org/10.1111/j.1558-5646.1985.tb00420.x

  18. Frøslev, T.G., Matheny, P.B. & Hibbett, D.S. (2005) Lower level relationships in the mushroom genus Cortinarius (Basidiomycota, Agaricales): A comparison of rpb1, rpb2, and ITS phylogenies. Molecular Phylogenetics and Evolution 37 (2): 602–618.  https://doi.org/10.1016/j.ympev.2005.06.016

  19. Frøslev, T.G., Brandrud, T.E. & Jeppesen, T.S. (2006) New species and combinations in Cortinarius subgenus Phlegmacium section Calochroi. Mycotaxon 97: 367–377. https://doi.org/10.1016/j.mycres.2006.05.012

  20. Frøslev, T.G., Jeppesen, T.S., Læssøe, T. & Kjøller, R. (2007) Molecular phylogenetics and delimitation of species in Cortinarius section Calochroi (Basidiomycota, Agaricales) in Europe. Molecular Phylogenetics and Evolution 44 (1): 217–227.  https://doi.org/10.1016/j.ympev.2006.11.013

  21. Frøslev, T.G., Jeppesen, T.S. & Dima, B. (2015) Cortinarius koldingensis a new species of Cortinarius, subgenus Phlegmacium related to Cortinarius sulfurinus. Mycological Progress 14: 73. https://doi.org/10.1007/s11557-015-1098-z

  22. Gardes, M. & Bruns, T.D. (1993) ITS primers with enhanced specificity for Basidiomycetes - application to the identification of mycorrhizae and rusts. Molecular Ecology 2 (2): 113–118.  https://doi.org/10.1111/j.1365-294X.1993.tb00005.x

  23. Garnica, S., Weiß, M., Oertel, B. & Oberwinkler, F. (2003) Phylogenetic relationships of European Phlegmacium species (Cortinarius, Agaricales). Mycologia 95: 1155–1170.  https://doi.org/10.1080/15572536.2004.11833025

  24. Garnica, S., Weiß, M., Oertel, B. & Oberwinkler, F. (2005) A framework for a phylogenetic classification in the genus Cortinarius (Basidiomycota, Agaricales) derived from morphological and molecular data. Canadian Journal of Botany 83 (11): 1457–1477. https://doi.org/10.1139/b05-107

  25. Garnica, S., Weiß, M., Oertel, B., Ammirati, J.F. & Oberwinkler, F. (2009) Phylogenetic relationships in Cortinarius, section Calochroi, inferred from nuclear DNA sequences. BMC Evolutionary Biology 9 (1): 1. https://doi.org/10.1186/1471-2148-9-1

  26. Garnica, S., Spahn, P., Oertel, B., Ammirati, J. & Oberwinkler, F. (2011) Tracking the evolutionary history of Cortinarius species in section Calochroi, with transoceanic disjunct distributions. BMC Evolutionary Biology 11 (1): 213.  https://doi.org/10.1186/1471-2148-11-213

  27. Garnica, S., Schön, M.E., Abarenkov, K., Riess, K., Liimatainen, K., Niskanen, T., Dima, B., Soop, K., Frøslev, T.G., Jeppesen, T.S., Peintner, U., Kühnert-Finkernagel, R., Brandrud, T.E., Saar, G., Oertel, B. & Ammirati, J.F. (2016) Determining threshold values for barcoding fungi: lessons from Cortinarius (Basidiomycota), a highly diverse and widespread ectomycorrhizal genus. FEMS Microbiology Ecology 92 (4): fiw045. https://doi.org/10.1093/femsec/fiw045

  28. Garrido-Benavent, I., Ballarà, J. & Mahiques, R. (2015) New insights into subg. Phlegmacium sect. Calochroi: adding morphological and molecular data from Mediterranean representatives, with special regard to Cortinarius prasinus, C. natalis and C. murellensis species complexes. Journal des J.E.C. 17: 38–78.

  29. Harrower, E., Ammirati, J.F., Cappuccino, A.A., Ceska, O., Kranabetter, K.M., Kroeger, P., SeaRa, L., Taylor, T. & Berbee, M.L. (2011) Cortinarius species diversity in British Columbia and molecular phylogenetic comparison with European specimen sequences. Botany 89: 799–810.  https://doi.org/10.1139/b11-065

  30. Henry, R. (1952) Les Scauri. Bulletin de la Société Mycologique de France 67 (3): 225–322.

  31. Henry, R (1992) Les cortinaires «Scauri» à lames jaunes (= Section Fulvi Moser et Horak p.p.). Documents mycologiques XXII (87): 17–41.

  32. Katoh, K., Misawa, K., Kuma, K., Miyata, T. (2002) MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic Acids Research 30 (14): 3059–3066.  https://doi.org/10.1093/nar/gkf436

  33. Kozlov, A.M., Darriba, D., Flouri, T., Morel, B. & Stamatakis, A. (2019) RA × ML-NG: A fast, scalable, and user-friendly tool for maximum likelihood phylogenetic inference. Bioinformatics 35 (21): 4453–4455.  https://doi.org/10.1093/bioinformatics/btz305

  34. Liimatainen, K., Niskanen, T., Dima, B., Kytövuori, I., Ammirati, J.F. & Frøslev, T.G. (2014) The largest type study of Agaricales species to date: bringing identification and nomenclature of Phlegmacium (Cortinarius) into the DNA era. Persoonia 33: 98–140.  https://doi.org/10.3767/003158514X684681

  35. Matheny, P.B., Liu, Y.J., Ammirati, J.F. & Hall, B.D. (2002) Using rpb1 sequences to improve phylogenetic inference among mushrooms (Inocybe, Agaricales). American Journal of Botany 89 (4): 688–698. https://doi.org/10.3732/ajb.89.4.688

  36. Matheny, P.B., Wang, Z., Binder, M., Curtis, J.M., Lim, Y.W., Nilsson, R.H., Hughes, K.W., Hofstetter, V., Ammirati, J.F., Schoch, C.L., Langer, E., Langer, G., McLaughlin, D.J., Wilson, A.W., Frøslev, T., Ge, Z.W., Kerrigan, R.W., Slot, J.C., Yang, Z.L., Baroni, T.J., Fischer, M., Hosaka, K., Matsuura, K., Seidl, M.T., Vauras, J. & Hibbett, D.S. (2007) Contributions of rpb2 and tef1 to the phylogeny of mushrooms and allies (Basidiomycota, Fungi). Molecular Phylogenetics and Evolution 43 (2): 430–451. https://doi.org/10.1016/j.ympev.2006.08.024

  37. Melot, J. (1990) Une classification du genre Cortinarius (Pers.) S. F. Gray. Documents Mycologiques 20 (80): 43–59.

  38. Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees In: Proceedings of the Gateway Computing Environments Workshop (GCE), 14 Nov. 2010, New Orleans, LA. pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129

  39. Moser, M. (1960) Die Gattung Phlegmacium (Sleimköpfe) Die Pilze Mitteleuropas, Band IV. Julius Klinkhardt, Bad Heilbrunn, 441 pp.

  40. Murray, M.G. & Thompson, W.F. (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8 (19): 4321–4325. https://doi.org/10.1093/nar/8.19.4321

  41. Ortega, A., Suárez-Santiago, V.N. & Reyes, J.D. (2008) Morphological and ITS identification of Cortinarius species (section Calochroi) collected in Mediterranean Quercus woodlands. Fungal Diversity 29: 73–88.

  42. Peintner, U, Bougher, N.L, Castellano, M.A, Moncalvo, J.M, Moser, M.M, Trappe, J.M. & Vilgalys, R. (2001) Multiple origins of sequestrate fungi related to Cortinarius (Cortinariaceae). American Journal of Botany 88 (12): 2168–2179. https://doi.org/10.2307/3558378

  43. Peintner, U., Moncalvo, J.M. & Vilgalys, R. (2004) Toward a better understanding of the infrageneric relationships in Cortinarius. Mycologia 96 (5): 1042–1058.  https://doi.org/10.1080/15572536.2005.11832904

  44. Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542.  https://doi.org/10.1093/sysbio/sys029

  45. Schmidt-Stohn, G., Brandrud, T.E. & Dima, B. (2017) Interessante Cortinarius-Funde der Journées européennes du Cortinaire 2016 in Borgsjö, Schweden. Journal des J.E.C. 19: 28–52.

  46. Simmons, M.P., Ochoterena, H. & Carr, T.G. (2001) Incorporation, relative homoplasy, and effect of gap characters in sequence-based phylogenetic analysis. Systematic Biology 50 (3): 454–462.  http://dx.doi.org/10.1080/106351501300318049

  47. Soop, K., Dima, B., Cooper, J.A., Duckchul, P. & Oertel, B. (2019) A phylogenetic approach to a global supraspecific taxonomy of Cortinarius (Agaricales) with an emphasis on the southern mycota. Persoonia 42: 261–290. https://doi.org/10.3767/persoonia.2019.42.10

  48. Stiller, J.W. & Hall, B.D. (1997) The origin of red algae: Implications for plastid evolution. PNAS 94 (9): 4520–4525.  https://doi.org/10.1073/pnas.94.9.4520

  49. Thiers, B. (2021) Index Herbariorum: a global directory of public herbaria and associated staff. USA: New York Botanical Garden’s Virtual Herbarium. Available at http://sweetgum.nybg.org/ih (last Accessed 24 February 2021)

  50. Trendel, J.M. (2020) Trois Cortinaires du clade Humolentes (section Calochroi s.l.) présents dans les forêts de feuillus du Nord de l’Alsace. Bulletin de la Société mycologique de Strasbourg 119: 13–23.

  51. Trimaille, G., Bidaud, A. & Valade, F. (2019) Quelques cortinaires rares récoltés lors de la session FMBDS à Évian-les-Bains (Haute-Savoie) en septembre 2017. Bulletin mycologique et botanique Dauphiné-Savoie 233: 5–21.

  52. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically ampliWed ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172 (8): 4238–4246. https:// 10.1128 / jb.172.8.4238-4246.1990

  53. Vizzini, A., Clericuzio, M., Boccardo, F. & Ercole, E. (2012) A new Cortinarius of section Calochroi (Basidiomycota, Agaricomycetes) from Mediterranean Quercus woodlands (Italy). Mycologia 104 (6): 1502–1509. https://doi.org/10.3852/12-072

  54. White, T.J., Bruns, T., Lee, S.S. & Taylor, J. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR Protocols: A Guide to Methods and Applications. Academic Press, New York, pp. 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1