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Published: 2021-09-21

Trichoderma dumbbelliforme sp. nov. an undescribed fungus of order Hypocreales from India

Division of Plant Pathology, ICAR- Indian Agricultural Research Institute, New Delhi, India-110012
Division of Plant Pathology, ICAR- Indian Agricultural Research Institute, New Delhi, India-110012
Division of Plant Pathology, ICAR- Indian Agricultural Research Institute, New Delhi, India-110012
Division of Plant Pathology, ICAR- Indian Agricultural Research Institute, New Delhi, India-110012
Ascomycetes Dumbbell shape Hypocreaceae Pleosporales Taxonomy Trichoderma dumbbelliforme Fungi


Trichoderma is an important genus of family Hypocreaceae, which contain nearly 375 known species. In current study, a new species Trichoderma dumbbelliforme collected from virgin forest soil of Nagaland and confirmed based on morphological and phylogenetic analyses. Morphologically, T. dumbbelliforme sp. nov. distinguished from other species of Trichoderma by producing nodules on sterile part of the conidiophores and pyriform to dumbbell shaped conidia with the size of 4.0–6.0×2.5–3.0µm and having two big guttulae at each end. Furthermore, the fungus was confirmed by molecular characterization using Translation elongation factor 1-α (tef-1) gene sequencing.  The phylogenetic relationship was established for generated sequence and many related ex-type sequences downloaded from NCBI GenBank by maximum-parsimony analysis. Tef-1 sequence data clarified the phylogenetic affinity to clade-1 of Trichoderma as per new authoritative guide line on molecular identification of Trichoderma. Phylogenetically, this new species of Trichoderma closely related to T. crassum, T. spirale, T. sulawesense, T. longisporum, T. virens, T. gelatinosum and T. gliocladium species. Therefore, the morphological comparisons were done with closely related species viz., T. crassum and T. spirale which lead to the conclusion that the collected taxon is new to science.


  1. Bae, H., Roberts, D.P., Lim, H.S., Strem, M.D., Park, S.C., Ryu, C.M., Melnick, R.L. & Bailey, B.A. (2011) Endophytic Trichoderma isolates from tropical environments delay disease onset and induce resistance against Phytophthora capsici in hot pepper using multiple mechanisms. Molecular Plant Microbe Interactions 24: 336–351.

  2. Bisset, J. (1991a) A revision of the genus Trichoderma II. Infrageneric classification. Canadian Journal of Botany 69: 2357–2372.

  3. Bisset, J. (1991b) A revision of the genus Trichoderma III.sect. pachybasium. Canadian Journal of Botany 69: 2373–2417.

  4. Bisset, J. (1991c) A revision of genus Trichoderma IV. Additional notes on section Longibrachiatum. Canadian Journal of Botany 69: 2418–2420.

  5. Bisset, J. (1992) Trichoderma atroviride. Canadian Journal of Botany 70: 639–641.

  6. Bissett, J., Gams, W., Jaklitsch, W. & Samuels, G.J. (2015) Accepted Trichoderma names in the year 2015. IMA Fungus 6 (2): 263–95.

  7. Cai, F. & Druzhinina, I.S. (2020) In honor of John Bissett: authoritative guidelines on molecular identification of Trichoderma.

  8. Carbone, I. & Kohn, L.M. (1999) A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91: 553–556.

  9. Chaverri, P., Castlebury, L.A., Samuels, G.J. & Geiser, D.M. (2003) Multilocus phylogenetic structure within the Trichoderma harzianum/Hypocrealixii complex. Molecular Phylogenetics and Evolution 27 (2): 302–313.

  10. Chaverri, P., Fabiano, B.R., Walter, J., Romina, G., Thomas, D. & Gary, J.S. (2015) Systematics of the Trichoderma harzianum species complex and the re-identification of commercial biocontrol strains. Mycologia 107 (3): 558–590.

  11. Choi, I. & Nisbett, R.E. (1998) Situational salience and cultural differences in the correspondence bias and the actor-observer bias. Personality and Social Psychology Bulletin 24: 949–960.

  12. Danielson, R.M. & Davey, C.B. (1973) The abundance of Trichoderma propagules and the distribution of species in forest soils. Soil Biology and Biochemistry 5: 486–494.

  13. Druzhinina, I., Koptchinski, A., Komon, M., Bissett, J., Szakacs, G. & Kubicek, C.P. (2005) An oligonucleotide barcode for species identification in Trichoderma and Hypocrea. Fungal Genetics and Biology 42: 813–828.

  14. Druzhinina, I.S., Komon´-Zelazowska, M., Ismaiel, A., Jaklitsch, W., Mullaw, T., Samuels, G.J. & Kubicek, C.P. (2012) Molecular phylogeny and species delimitation in the section Longibrachiatum of Trichoderma. Fungal Genetics and Biology 49 (5): 358–368.

  15. Harman, G.E., Howell, C.R., Viterbo, A., Chet, I. & Lorito, M. (2004) Trichoderma species opportunistic, avirulent plant symbionts. Nature Reviews Microbiology 2 (1): 43–56.

  16. Jaklitsch, W.M., Samuels, G., Ismaiel, A. & Voglmayr, H. (2013) Disentangling the Trichoderma viridescens complex. Persoonia 31 (1): 112–146.

  17. Kiffer, E. & Morelet, E. (2000) The Deuteromycetes Mitosporic Fungi Classification and Generic Keys. Science Publications Inc., USA, 1152. https://doi.10.1201/9781482294194

  18. Klein, D. & Eveleigh, D.E. (1998) Ecology of Trichoderma. In: Kubicek CP, Harman GE, editors. Trichodermaand Gliocladium. Vol. 1. Basic Biology, Taxonomy and Genetics. London: Taylor and Francis Ltd. pp. 57–74.

  19. Nagamani, A., Mahoharachary, C. & Agrawal, D. (2002) Monographic contribution on Trichoderma pers. ex Fr., Today and Tomorrow publications, New Delhi. pp.49.

  20. Novakova, A., Kubátová, A., Valinová, Š., Hubka, V. & Kolařík, M. (2015) Trichoderma fassatiae, a new species from the section Pachybasium isolated from soil in the Czech Republic. Czech Mycology 67 (2): 227–231.

  21. Ospina-Giraldo, M., Royse, D., Thon, M., Chen, X. & Romaine, C. (1998) Phylogenetic relationships of Trichoderma harzianum causing mushroom green mold in Europe and North America to other species of Trichoderma from world-wide sources. Mycologia 90 (1): 76–81.

  22. Persoon, C.H. (1794) Disposita methodical fungorum. Römer’sNeues Mag Bot. 1, 81–128.

  23. Prameeladevi, T., Prabhakaran, N., Kamil, D., Pandey, P. & Borah, J.L. (2012) Characterization of Indian native isolates of Trichoderma spp. and assessment of their bio-control efficiency against plant pathogens. African Journal of Biotechnology 1185: 15150–15160. https://doi:10.5897/AJB12.2007

  24. Ridgway, R. (1912) Colorstandards and color nomenclature. Washington, D.C. 44 pp. 53.

  25. Rifai, M.A. (1969) A revision of the genus Trichoderma. Mycological Papers 116: 1–56.

  26. Rogers, S.O. &Bendich, A.J. (1994) Extraction of total cellular DNA from plants, algae and fungi. In: Gelvin SB, Schilperoort RA (eds.) Plant molecular biology manual. Kluwer Academic, Boston, pp D1: 1–8

  27. Samuels, G.J., Dodd, S.L., Gams, W., Castlebury, L.A. & Petrini, O. (2002) Trichoderma species associated with the green mold epidemic of commercially grown Agaricusbisporus. Mycologia 94 (1): 146–170.

  28. Samuels, G.J., Dodd, S.L., Lu, B.S., Petrini, O., Schroers, H.J. & Druzhinina, I.S. (2006) The Trichoderma koningii aggregate species. Studies in Mycology 56: 67–133.

  29. Samuels, G.J., Ismaiel, A., Temesgen, B.M., Szakacs, G., Irina, S.D., Christian, P.K. & Walter, M.J. (2012) The Longibrachiatum Clade of Trichoderma: a revision with new species. Fungal Diversity 55: 77–108.

  30. Samuels, G.J., Ismaiel, A., Mulaw, T.B., Szakacs, G., Druzhinina, I.S., Kubicek, C.P., Jaklitsch, W.M. & Samuels, G.J. (1996) Trichoderma: A review of biology and systematics of the genus. Mycologycal Research 100: 923–935.

  31. Tamura, K. & Nei, M. (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution 10 (3): 512–526.

  32. Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology & Evolution 30: 2725–2729.  https://doi:10.1093/molbev/mst197

  33. Taylor, J.W., Jacobson, D.J., Kroken, S., Kasuga, T., Geiser, D.M., Hibbett, D.S. & Fisher, M.C. (2000) Phylogenetic species recognition and species concepts in fungi. Fungal Genetics and Biology 31 (1): 21–32. https://doi:10.1006/fgbi.2000.1228

  34. Thakur, A.K. & Norris, R.V. (1928) A biochemical study of some soil-fungi with special reference to ammonia production. Journal of the Indian Institute of Science 11A: 141–147.