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Article
Published: 2022-01-06

New species, Parahelicomyces yunnanensis sp. nov. and Tubeufia nigroseptum sp. nov. from freshwater habitats in Yunnan, China

College of Agriculture and Biological Sciences, Dali University, Dali 671003, Yunnan, P.R. China
College of Agriculture and Biological Sciences, Dali University, Dali 671003, Yunnan, P.R. China; Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand; School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand
College of Agriculture and Biological Sciences, Dali University, Dali 671003, Yunnan, P.R. China; Center of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai 57100, Thailand; Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand
School of Food and Pharmaceutical Engineering, Guizhou Institute of Technology, Guiyang 550025, Guizhou, P.R. China
College of Agriculture and Biological Sciences, Dali University, Dali 671003, Yunnan, P.R. China
College of Agriculture and Biological Sciences, Dali University, Dali 671003, Yunnan, P.R. China
2 new species Freshwater fungi Helicosporous hyphomycetes Phylogeny Taxonomy Fungi

Abstract

Diversity of lignicolous freshwater fungi in northwestern Yunnan, China is currently being studied. Four fresh collections of tubeufiaceous taxa were collected and identified. Among of them, Parahelicomyces yunnanensis sp. nov and Tubeufia nigroseptum sp. nov. are introduced as new species based on morphology and molecular phylogenetic analysis of combined ITS, SSU, TEF1-α and RPB2 sequence data. The detailed descriptions and illustrations of the new species are provided, as well as the morphological comparison with similar taxa are discussed. Two strains of Neohelicomyces aquaticus and Tubeufia cylindrothecia are provided.

References

  1. Barr, M.E. (1979) A classification of Loculoascomycetes. Mycologia 71: 935–957. https://doi.org/10.1080/00275514.1979.12021099

  2. Barr, M.E. (1980) On the family Tubeufiaceae (Pleosporales). Mycotaxon 12: 137–167.

  3. Boonmee, S., Zhang, Y., Chomnunti, P., Chukeatirote, E., Tsui, C.K.M., Bahkali, A.H. & Hyde, K.D. (2011) Revision of lignicolous Tubeufiaceae based on morphological reexamination and phylogenetic analysis. Fungal Diversity 51: 63–102. https://doi.org/10.1007/s13225-011-0147-4

  4. Boonmee, S., Rossman, A.Y., Liu, J.K., Li, W.J., Dai, D.Q., Bhat, D.J., Jones, E.B.G., McKenzie, E.H.C., Xu, J.C. & Hyde, K.D. (2014) Tubeufiales, ord. nov., integrating sexual and asexual generic names. Fungal Diversity 68: 239–298.  https://doi.org/10.1007/s13225-014-0304-7

  5. Brahamanage, R.S., Lu, Y.Z., Bhat, D.J., Wanasinghe, D.N., Yan, J.Y., Hyde, K.D. & Boonmee, S. (2017) Phylogenetic investigations on freshwater fungi in Tubeufiaceae (Tubeufiales) reveals the new genus Dictyospora and new species Chlamydotubeufia aquatica and Helicosporium flavum. Mycosphere 8: 917–933. https://doi.org/10.5943/mycosphere/8/7/8

  6. Cai, L., Tsui, C.K.M., Zhang, K.Q. & Hyde, K.D. (2002) Aquatic fungi from Lake Fuxian, Yunnan, China. Fungal Diversity 9: 57–70. https://doi.org/10.1017/S0953756202005658

  7. Cai, L., Zhang, K., McKenzie, E.H.C. & Hyde, K.D. (2003) Freshwater fungi from bamboo and wood submerged in the Liput River in the Philippines. Fungal Diversity 13: 1–12. https://doi.org/10.1016/S1567-1356(03)00107-7

  8. Cai, L., Jeewon, R. & Hyde, K.D. (2006) Phylogenetic investigations of Sordariaceae based on multiple gene sequences and morphology. Mycological Research 110: 137–150.  https://doi.org/10.1016/j.mycres.2005.09.014

  9. Chaiwan, N., Lu, Y.Z., Tibpromma, S., Bhat, D.J., Hyde, K.D. & Boonmee, S. (2017) Neotubeufa gen. nov. and Tubeufa guangxiensis sp. nov. (Tubeufiaceae) from freshwater habitats. Mycosphere 8: 1443–1456. https://doi.org/10.5943/mycosphere/8/9/9

  10. Chomnunti, P., Hongsanan, S., Aguirre-Hudson, B., Tian, Q., Peršoh, D., Dhami, M.K., Alias, A.S., Xu, J.C., Liu, X.Z., Stadler, M. & Hyde, K.D. (2014) The sooty moulds. Fungal Diversity 66: 1–36. https://doi.org/10.1007/s13225-014-0278-5

  11. Crous, P.W., Schumacher, R.K., Akulov, A., Thangavel, R., Hernández-Restrepo, M., Carnegie, A.J., Cheewangkoon, R. & Wingfield, M.J. (2019) New and Interesting Fungi. 2. Fungal Systematics and Evolution 3: 57–134. https://doi.org/10.3114/fuse.2019.03.06

  12. Dai, D.Q., Phookamsak, R., Wijayawardene, N.N., Li, W.J., Bhat, D.J. & Xu, J.C. (2017) Bambusicolous fungi. Fungal Diversity 82 (1): 1–105. https://doi.org/10.1007/s13225-016-0367-8

  13. Doilom, M., Dissanayake, A.J., Wanasinghe, D.N., Boonmee, S., Liu, J.K., Bhat, D.J., Taylor, J.E., Bahkali, A.H., McKenzie, E.H.C. & Hyde, K.D. (2017) Microfungi on Tectona grandis (teak) in Northern Thailand. Fungal Diversity 82: 107–182. https://doi.org/10.1007/s13225-016-0368-7

  14. Dong, W., Wang, B., Hyde, K.D., McKenzie, E.H.C., Raja, H.A., Tanaka, K., Abdel‑Wahab, M.A., Abdel‑Aziz, F.A., Doilom, M., Phookamsak, R., Hongsanan, S., Wanasinghe, D.N., Yu, X.D., Wang, G.N., Yang, Hao., Yang, J., Thambugala, K.M., Tian, Q., Luo, Z.L., Yang, J.B., Miller, A.N., Fournier, J., Boonmee, S., Hu, D.M., Nalumpang, S. & Zhang, H. (2020) Freshwater Dothideomycetes. Fungal Diversity 105: 319–575. https://doi.org/10.1007/s13225-020-00463-5

  15. Fang, C., Lu, Y.Z., Kang, J.C., Wang, L., Lei, B.X. & Chen, L.Z. (2019) Evaluation of bioactivity of secondary metabolites from fungi of Tubeufiaceae. Mycosystema 38 (4): 560–574. https://doi.org/10.1007/s13205-016-0518-3

  16. Hall, T.A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98. https://doi.org/10.12691/ajmr-6-4-3

  17. Hanada, T., Sato, T., Arioka, M., Uramoto, M. & Yamasaki, M. (1996) Purification and characterization of a 15kDa protein (p15) produced by Helicosporium that exhibits distinct effects on neurite outgrowth from cortical neurons and PC12 cells. Biochemical and Biophysical Research Communications 228 (1): 209–21. https://doi.org/10.1006/bbrc.1996.1641

  18. Hyde, K.D. & Goh, T.K. (1998) Fungi on submerged wood in Lake Barrine, north Queensland, Australia. Mycological Research 102: 739–749. https://doi.org/10.1017/S0953756297005868

  19. Hyde, K.D., Fryar, S., Tian, Q., Bahkali, A.H. & Xu, J.C. (2016a) Lignicolous freshwater fungi along a northsouth latitudinal gradient in the Asian/Australian region; can we predict the impact of global warming on biodiversity and function? Fungal Ecology 19: 190–200. https://doi.org/10.1016/j.funeco.2015.07.002

  20. Hyde, K.D., Hongsanan, S., Jeewon, R., Bhat, D.J., McKenzie, E.H.C., Jones, E.B.G., Phookamsak, R., Ariyawansa, H.A., Boonmee, S., Zhao, Q., Abdel-Aziz, F.A., Abdel-Wahab, M.A., Banmai, S., Chomnunti, P., Cui, B.K., Daranagama, D.A., Das, K., Dayarathne, M.C., de Silva, N.I., Dissanayake, A.J., Doilom, M., Ehanayaka, A.H., Gibertoni, T.B., Góes-Neto, A., Huang, S.K., Jayasiri, S.C., Jayawardena, R.S., Konta, S., Lee, H.B., Li, W.J., Lin, C.G., Liu, J.K., Lu, Y.Z., Luo, Z.L., Manawasinghe, I.S., Manimohan, P., Mapook, A., Niskanen, T., Norphanphoun, C., Papizadeh, M., Perera, R.H., Phukhamsakda, C., Richter, C., de Santiago, A.L.C.M., Drechsler-Santos, E.R., Senanayake, I.C., Tanaka, K., Tennakoon, T.M.D.S., Thambugala, K.M., Tian, Q., Tibpromma, S., Thongbai, B., Vizzini, A., Wanasinghe, D.N., Wijayawardene, N.N., Wu, H.X., Yang, J., Zeng, X.Y., Zhang, H., Zhang, J.F., Bulgakov, T.S., Camporesi, E., Bahkali, A.H., Amoozegar, M.A., Araujo-Neta, L.S., Ammirati, J.F., Baghela, A., Bhatt, R.P., Bojantchev, D., Buyck, B., de Silva, G.A., de Lima, C.L.F., de Oliveira, R.J.V., de Souza, C.A.F., Dai, Y.C., Dima, B., Duong, T.T., Ercole, E., Mafalda-Freire, F., Ghosh, A., Hashimoto, A., Kamolhan, S., Kang, J.C., Karunarathna, S.C., Kirk, P.M., Kytovuori, I, Lantieri, A., Liimatainen, K., Liu, Z.Y., Liu, X.Z, Lücking, R., Medardi, G., Mortimer, P.E., Nguyen, T.T.T., Promputtha, I., Raj, K.N.A., Reck, M.A., Lumyong, S., Shahzadeh-Fazeli, S.A., Stadler, M., Soudi, M.R., Su, H.Y., Takahashi, T., Tangthirasunun, N., Uniyal, P., Wang, Y., Wen, T.C., Xu, J.C., Zhang, Z.K., Zhao, Y.C., Zhou, J.L. & Zhu, L. (2016b) Fungal diversity notes 367-490: taxonomic and phylogenetic contributions to fungal taxa. Fungal Diversity 80: 1–270. https://doi.org/10.1007/s13225-016-0373-x

  21. Hyde, K.D., Norphanphoun, C., Abreu, V.P., Bazzicalupo, A., Chethana, K.W.T., Clericuzio, M., Dayarathne, M.C., Dissanayake, A.J., Ekanayaka, A.H., He, M.Q., Hongsanan, S., Huang, S.K., Jayasiri, S.C., Jayawardena, R.S., Karunarathna, A., Konta, S., Kušan, I., Lee, H., Li, J., Lin, C.G., Liu, N.G., Lu, Y.Z., Luo, Z.L., Manawasinghe, I.S., Mapook, A., Perera, R.H., Phookamsak, R., Phukhamsakda, C., Siedlecki, I., Soares, A.M., Tennakoon, D.S., Tian, Q., Tibpromma, S., Wanasinghe, D.N., Xiao, Y.P., Yang, J., Zeng, X.Y., Abdel-Aziz, F.A., Li, W.J., Senanayake, I.C., Shang, Q.J., Daranagama, D.A., De Silva, N.I., Thambugala, K.M., Abdel-Wahab, M.A., Bahkali, A.H., Berbee, M.L., Boonmee, S., Bhat, D.J., Bulgakov, T.S., Buyck, B., Camporesi, E., Castañeda-Ruiz, R.F., Chomnunti, P., Doilom, M., Dovana, F., Gibertoni, T.B., Jadan, M., Jeewon, R., Jones, G.E.B., Kang, J.C., Karunarathna, S.C., Lim, Y.W., Liu, J.K., Liu, Z.Y., Plautz, H.L. Jr., Lumyong, S., Maharachchikumbura, S.S.N., Matočec, N., Mckenzie, E.H.C., Mešić, A., Miller, D., Pawłowska, J., Pereira, O.L., Promputtha, I., Romero, A.I., Ryvarden, L., Su, H.Y., Suetrong, S., Tkalčec, Z., Vizzini, A., Wen, T.C., Wisitrassameewong, K., Wrzosek, M., Xu, J.C., Zhao, Q., Zhao, R.L. & Mortimer, P.E. (2017) Fungal diversity notes 603-708: taxonomic and phylogenetic notes on genera and species. Fungal Diversity 87: 1–235. https://doi.org/10.1007/s13225-017-0391-3

  22. Ho, W.H., Hyde, K.D. & Hodgkiss, I.J. (2001) Fungal communities on submerged wood from streams in Brunei, Hong Kong, and Malaysia. Mycological Research 105: 1492–1501. https://doi.org/10.1017/S095375620100507X

  23. Hongsanan, S., Hyde, K.D., Phookamsak, R., Wanasinghe, D.N., McKenzie, E.H.C., Sarma, V.V., Lücking, R., Boonmee, S., Bhat, D.J., Liu, N.J., Tennakoon, D.S., Pem, D., Karunarathna, N., Jiang, S.H., Jones, G.E.B., Phillips, A.J.L., Manawasinghe, I.S., Tibpromma, S., Jayasiri, S.C., Sandamali, D., Jayawardena, R.S., Wijayawardene, N.N., Ekanayaka, A.H., Jeewon, R., Lu, Y.Z., Phukhamsakda, C., Dissanayake, A.J., Zeng, X.Y., Luo, Z.L., Tian, Q., Thambugala, K.M., Dai, D., Samarakoon, M.C., Chethana, K.W.T., Ertz, D., Doilom, M., Liu, J.K., Pérez‑Ortega, S., Suija, A., Senwanna, C., Wijesinghe, S.N., Niranjan, M., Zhang, S.N., Ariyawansa, H.A., Jiang, H.B., Zhang, J.F., Norphanphoun, C., de Silva, N.I., Thiyagaraja, V., Zhang, H., Bezerra, J.D.P., Miranda‑González, R., Aptroot, A., Kashiwadani, H., Harishchandra, D., Sérusiaux, E., Abeywickrama, P.D., Bao, D.F., Devadatha, B., Wu, H.X., Moon, K.H., Gueidan, C., Schumm, F., Bundhun, D., Mapook, A., Monkai, J., Bhunjun, C.S., Chomnunti, P., Suetrong, S., Chaiwan, N., Dayarathne, M.C., Yang, J., Rathnayaka, A.R., Xu, J.C., Zheng, J., Liu, G., Feng, Y. & Xie, N. (2020) Refned families of Dothideomycetes: orders and families incertae sedis in Dothideomycetes. Fungal Diversity 105: 17–318. https://doi.org/10.1007/s13225-020-00462-6

  24. Hsieh, S.Y., Goh, T.K. & Kuo, C.H. (2021) New species and records of Helicosporium sensu lato from Taiwan, with a reflection on current generic circumscription. Mycological Progress 20: 169–190. https://doi.org/10.1007/s11557-020-01663-8

  25. Itazaki, H., Nagashima, K., Sugita, K., Yoshida, H., Kawamura, Y., Yasuda, Y., Matsumoto, K., Ishii, K., Uotani, N., Nakai, H., Terui, A., Yoshimatsu, S., Ikenishi, Y. & Nakagawa, Y. (1990) Solation and structural elucidation of new cyclotetrapeptides, trapoxins A and B, having detransformation activities as antitumor agents. The Journal of Antibiotics 43: 1524–1532.

  26. Jayasiri, S.C., Hyde, K.D., Ariyawansa, H.A., Bhat, D.J., Buyck, B., Cai, L., Dai, Y.C., Abd-Elsalam, K.A., Ertz, D., Hidayat, I., Jeewon, R., Gareth Jones, E.B., Bahkali, A.H., Karunarathna, S.C., Liu, J.K., Luangsa-ard, J., Thorsten Lumbsch, H., Maharachchikumbura, S.S.N., McKenzie, E.H.C., Moncalvo, J.M., Ghobad-Nejhad, M., Nilsson, H., Pang, K.L., Pereira, O.L., Phillips, A.J.L., Raspé, O., Rollins, A.W., Romero, A.I., Etayo, J., Selçuk, F., Stephenson, S.L., Suetrong, S., Taylor, J.E., Tsui, C.K.M., Vizzini, A., Abdel-Wahab, M.A., Wen, T.C., Boonmee, S., Dai, D.Q., Daranagama, D.A., Dissanayake, A.J., Ekanayaka, A.H., Fryar, S.C., Hongsanan, S., Jayawardena, R.S., Li, W.J., Perera, R.H., Phookamsak, R., de Silva, N.I., Thambugala, K.M., Tian, Q., Wijayawardene, N.N., Zhao, R.L., Zhao, Q., Kang, J.C. & Promputtha, I. (2015) The faces of fungi database: fungal names linked with morphology, phylogeny and human impacts. Fungal Diversity 74: 3–18. https://doi.org/10.1007/s13225-015-0351-8

  27. Jayasiri, S.C., Hyde, K.D., Jones, E.B.G., McKenzie, E.H.C., Jeewon, R., Phillips, A.J.L., Bhat, D.J., Wanasinghe, D.N., Liu, J.K., Lu, Y.Z., Kang, J.C., Xu, J. & Karunarathna, S.C. (2019) Diversity, morphology and molecular phylogeny of Dothideomycetes on decaying wild seed pods and fruits. Mycosphere 10 (1): 1–186 https://doi 10.5943/mycosphere/10/1/1

  28. Jeewon, R., Hyde, K.D. (2016) Establishing species boundaries and new taxa among fungi: recommendations to resolve taxonomic ambiguities. Mycosphere 7: 1669–1677. https://doi: 10.5943/mycosphere/7/11/4

  29. Karunarathna, S.C., Udayanga, D., Maharachchikumbura, S.N., Pilkington, M. & Hyde, K.D. (2012) Current status of knowledge of Sri Lankan mycota. Current Research in Environmental & Applied Mycology 2: 18–29.  https://doi: 10.5943/cream/2/1/2

  30. Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30: 772–780. https://doi: 10.1093/molbev/mst010

  31. Kodsueb, R., Jeewon, R., Vijaykrishna, D., McKenzie, E.H.C., Lumyong, P., Lumyong, S. & Hyde, K.D. (2006) Systematic revision of Tubeufiaceae based on morphological and molecular data. Fungal Diversity 21: 105–130.

  32. Kuo, C.H. & Goh, T.K. (2018a) A new species and a new record of Helicomyces from Taiwan. Mycoscience 59 (5): 433–440. https://doi.org/10.1016/j.myc.2018.04.002

  33. Kuo, C.H. & Goh, T.K. (2018b) Two new species of helicosporous hyphomycetes from Taiwan. Mycological progress 17: 557–569.

  34. Liu, J.K., Phookamsak, R., Doilom, M., Wikee, S., Li, Y.M., Ariyawansha, H., Boonmee, S., Chomnunti, P., Dai, D.Q., Bhat, D.J., Romero, A.I., Zhuang, W.Y., Monkai, J., Jones, E.B.G., Chukeatirote, E., Thida, W.K., Zhao, Y.C., Wang, Y. & Hyde, K.D. (2012) Towards a natural classification of Botryosphaeriales. Fungal Diversity 57: 149–210. https://doi.org10.1007/s13225-012-0207-4

  35. Liu, J.K., Lu, Y.Z., Cheewangkoon, R. & To-Anun, C. (2018) Phylogeny and morphology of Helicotubeufia gen. nov, with three new species in Tubeufiaceae from aquatic habitats. Mycosphere 9: 495–509. https://doi.org/10.5943/MYCOSPHERE/9/3/4

  36. Liu, N.G., Lu, Y.Z., Bhat, D.J., McKenzie, E.H.C., Saisamorn, L., Jumpathong, J. & Liu, J.K. (2019) Kevinhydea brevistipitata gen. et sp. nov. and Helicoma hydei sp. nov. (Tubeufaceae) from decaying wood habitats. Mycological progress 18: 671–682. https://doi.org10.1007/s11557-019-01480-8

  37. Liu, Y.J., Whelen, S. & Hall, B.D. (1999) Phylogenetic relationships among ascomycetes: evidence from an RNA polymerase II subunit. Molecular Biology and Evolution 16: 1799–1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092

  38. Lu, Y.Z., Boonmee, S., Bhat, D.J., Hyde, K.D. & Kang, J.C. (2017a) Helicosporium luteosporum sp. nov. and Acanthohelicospora aurea (Tubeufiaceae, Tubeufiales) from terrestrial habitats. Phytotaxa 319: 241–253. https://doi.org10.11646/PHYTOTAXA.319.3.3

  39. Lu, Y.Z., Boonmee, S., Dai, D.Q., Liu, J.K., Hyde, K.D., Bhat, D.J. & Kang, J.C. (2017b) Four new species of Tubeufia (Tubeufiaceae, Tubeufiales) from Thailand. Mycological Progress 16: 403–417. https://doi.org/10.1007/s11557-017-1280-6

  40. Lu, Y.Z., Boonmee, S., Liu, J.K., Hyde, K.D., Bhat, D.J., Eungwanichayapant, P.D. & Kang, J.C. (2017c) Novel Neoacanthostigma species from aquatic habitats. Cryptogamie Mycologie 38: 169–190. https://doi.org/10.7872/crym/v38.iss2.2017.169

  41. Lu, Y.Z., Liu, J.K., Hyde, K.D., Jeewon, R., Kang, J.C., Fan, C., Boonmee, S., Bhat, D.J., Luo, Z.L. & Lin, C.G. & Eungwanichayapant, P.D. (2018) A taxonomic reassessment of Tubeufales based on multi-locus phylogeny and morphology. Fungal Diversity 92: 131–344. https://doi.org/10.1007/s13225-018-0411-y

  42. Lu, Y.Z., Liu, J.K. & Hyde, K.D. (2020) (2744) Proposal to conserve Pseudohelicomyces Y.Z. Lu & al. (Tubeufiaceae) against Pseudohelicomyces Garnica & E. Valenz. (Hymenogastraceae). Taxon 69 (3): 615–616. https://doi.org/10.1002/tax.12268

  43. Luo, Z.L., Bhat, D.J., Jeewon, R., Boonmee, S., Bao, D.F., Zhao, Y.C., Chai, H.M., Su, H.Y., Su, X.J. & Hyde, K.D. (2017) Molecular phylogeny and morphological characterization of asexual fungi (Tubeufiaceae) from freshwater habitats in Yunnan, China. Cryptogamie Mycologie 38: 27–53. https://doi.org/10.7872/crym/v38.iss1.2017.27

  44. Luo, Z.L., Hyde, K.D., Liu, J.K., Bhat, D.J., Bao, D.F., Li, W.L., Su, X.J., Yang, X.Y. & Su, H.Y. (2018) Lignicolous freshwater fungi from China II: novel Distoseptispora (Distoseptisporaceae) species from northwestern Yunnan Province and a suggested unified method for studying lignicolous freshwater fungi. Mycosphere 9: 444–461.  https://doi: 10.5943/mycosphere/9/3/2

  45. Mao, Z.L., Sun, W.B., Fu, L.Y., Luo, H.Y., Lai, D.W. & Zhou, L.G. (2014) Natural dibenzo-α-pyrones and their bioactivities. Molecules, 19 (4): 5088–5108.  https://doi.org/10.3390/molecules19045088

  46. Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: 2010 Gateway Computing Environments Workshop (GCE). pp. 1–8. https://doi.org/10.1109/GCE.2010.5676129

  47. Nguanhom, J., Cheewangkoon, R., Groenewald, J.Z., Braun, U., To-Anun, C. & Crous, P.W. (2015) Taxonomy and phylogeny of Cercospora spp. from Northern Thailand. Phytotaxa 233: 27–48.  https://doi.org/:10.11646/phytotaxa.233.1.2

  48. Nylander, J.A.A. (2004) MrModeltest v2 Program distributed by the author. Evolutionary Biology Centre, Uppsala University: Uppsala, Sweden.

  49. Ohtsu, Y., Sasamura, H., Shibata, T., Nakajima, H., Hino, M. & Fujii, T. (2003b) The novel gluconeogenesis inhibitors FR225659 and related compounds that originate from Helicomyces sp. No. 19353. II. Biological profiles. The Journal of Antibiotics 56 (8): 689–693.  https://doi.org/:10.7164/ANTIBIOTICS.56.682

  50. Ohtsu, Y., Sasamura, H., Tsurumi, Y., Yoshimura, S., Takase, S., Hashimoto, M., Shibata, T., Hino, M. & Fujii, T. (2003a) The novel gluconeogenesis inhibitors FR225659 and related compounds that originate from Helicomyces sp. No. 19353. I. Taxonomy, fermentation, isolation and physico-chemical properties. The Journal of Antibiotics 56 (8): 682–688.  http://doi.org/10.7164/antibiotics.56.689

  51. Phookamsak, R., Hyde, K.D., Jeewon, R., Bhat, D.J., Jones, E.B.G., Maharachchikumbura, S.S.N., Raspé, O., Karunarathna, S.C., Wanasinghe, D.N., Hongsanan, S., Doilom, M., Tennakoon, D.S., Machado, A.R., Firmino, A.L., Ghosh, A., Karunarathna, A., Mesic, A., Dutta, A.K., Thongbai, B., Devadatha, B., Norphanphoun, C., Senwanna, C., Wei, DP., Pem, D., Achah, F.K., Wang, G.N., Jiang, H.B., Madrid, H., Lee, H.B., Goonasekara, I.D., Manawasinghe, I.S., Kusan, I., Cano, J., Gene, J., Li, JF., Das, K., Acharya, K., Raj, K.N.A., Latha, K.P.D., Chethana, K.W.T., He, M.Q., Duenas, M., Jadan, M., Martin, M.P., Samarakoon, M.C., Dayaratne, M.C., Raza, M., Park, M.S., Tellaria, M.T., Chaiwan, N., Matocec, N., de Silva, N.I., Pereira, O.L., Singh, S.K., Vadthanarat, S., Oh, S.Y., Huang, S.K., Rana, S., Konta, S., Paloi, S., Jayasiri, S.C., Jeon, S.J., Mehmood, T., Gibertoni, T.B., Nguyenm, T.T.T., Singhe, U., Thiyagaraja, V., Sharma, V.V., Dong, W., Yu, X.D., Lu, Y.Z., Lim, Y.W., Chen, Y., Tkalcec, Z., Zhang, J.F., Luo, Z.L., Daranagama, D.A., Thambugala, K.M., Tibpromma, S., Camporesi, E., Bulgokov, T.B., Dissanayake, A.J., Senanayake, I.C., Dai, D.Q., Tang, L.Z., Khan, S., Zhang, H., Promputta, I., Cai, L., Chomnuti, P., Zhao, R.L., Lumyong, S., Boonmee, S., Wen, T.C., Mortimer, P.E. & Xu, J.C. (2019) Fungal diversity notes 929–1035: taxonomic and phylogenetic contributions on genera and species of fungi. Fungal Diversity 95: 1–273.  https://doi.org/10.1007/s13225-019-00421-w

  52. Penzig, O.A.J. & Saccardo, P.A. (1897) Diagnoses fungorum novorum in Insula Java collectorum. Series secunda. Malpighia 11: 491–530.

  53. Promputtha, I. & Miller, A.N. (2010) Three new species of Acanthostigma (Tubeufiaceae, Dothideomycetes) from Great Smoky Mountains National Park. Mycologia 102: 574–587. http://doi.org/10.3852/09-051

  54. Rambaut, A. (2014) FigTree 1.4.2. Avaialble from: http://tree.bio.ed.ac.uk/software/ figtree (accessed 9 July 2014)

  55. Ranala, B. & Yang, Z. (1996) Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference. Journal of Molecular Evolution 43: 304–311. https://doi.org/10.1007/BF02338839

  56. Rajeshkumar, K.C., Bhat, D.J., Lad, S.S., Wijayawardene, N.W., Singh, S.K., Pandkar, M.R., Maurya, D.K., Ashtekar, N.D. & Hyde, K.D. (2018) Morphology and phylogeny of Tamhinispora srinivasanii sp. nov. (Tubeufiaceae) from northern Western Ghats, India. Phytotaxa 346: 113–120.  https://doi.org/10.11646/PHYTOTAXA.346.1.7

  57. Rajeshkumar, K.C., Hyde, K.D., Wijayawardene, N.N., Madrid, H. & Fatima, S. (2019) Tubeufia sahyadriensis (Tubeufiaceae), a new dictyosporous anamorph from the Western Ghats, India. Phytotaxa 423 (3): 171–181. https://doi.org/10.11646/phytotaxa.423.3.5

  58. Ronquist, F., Huelsenbeck, J.P. (2003) MrBayes3: bayesian phylogenetic inference under mixed models. Bioinformatics 19: 1572–1574.  https://doi.org/10.1093/ bioinformatics/btg180

  59. Sánchez, R.M., Miller, A.N. & Bianchinotti, M.V. (2012) A new species of Acanthostigma (Tubeufiaceae, Dothideomycetes) from the southern hemisphere. Mycologia 104 (1): 223–231. https://doi.org/10.3852/11-105

  60. Schoch, C.L., Shoemaker, R.A., Seifert, K.A., Hambleton, S., Spatafora, J.W. & Crous, P.W. (2006) A multigene phylogeny of the Dothideomycetes using four nuclear loci. Mycologia 98 (6): 1041–1052. https://doi.org/10.1080/15572536.2006.11832632

  61. Slippers, B., Boissin, E., Phillips, A.J.L., Groenewald, J.Z., Lombard, L., Wingfield, M.J., Postma, A., Burgess,T. & Crous, P.W. (2013) Phylogenetic lineages in the Botryosphaeriales: a systematic and evolutionary framework. Study in Mycology 76 (1): 31–49. https://doi.org/10.3114/sim0020

  62. Stamatakis, A. (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22: 2688–2690.  https://doi.org/10.1093/bioinformatics/btl446

  63. Stamatakis, A., Hoover, P. & Rougemont, J. (2008) A rapid bootstrap algorithm for the RAxML web-servers. Systematic Biology 75: 758–771. https://doi.org/10.1080/10635150802429642

  64. Su, H.Y., Udayanga, D., Luo, Z.L., Manamgoda, D.S., Zhao, Y.C., Yang, J., Liu, X.Y., Mckenzie, E.H.C. & Hyde, K.D. (2015) Hyphomycetes from aquatic habitats in Southern China: Species of Curvularia (Pleosporaceae) and Phragmocephala (Melannomataceae). Phytotaxa 226 (3): 201–216.  https://doi.org/10.11646/PHYTOTAXA.226.3.1

  65. Thambugala, K.M., Daranagama, D.A., Phillips, A.J.L., Bulgakov, T.S., Bhat, D.J., Camporesi, E., Bahkali, A.H., Eungwanichayapant, P.D., Liu, Z.Y. & Hyde, K.D. (2017) Microfungi on Tamarix. Fungal Diversity 82: 239–306. https://doi.org/10.1007/s13225-016-0371-z

  66. Tibpromma, S., Hyde, K.D., McKenzie, E.H.C., Bhat, D.J., Phillips, A.J.L., Wanasinghe, D.N., Samarakoon, M.C., Jayawardena, R.S., Dissanayake, A.J., Tennakoon, D.S., Doilom, M., Phookamsak, R., Tang, A.M.C., Xu, J., Mortimer, P.E., Promputtha, I., Maharachchikumbura, S.S.N., Khan, S. & Karunarathna, S.C. (2018) Fungal diversity notes 840–928: micro-fungi associated with Pandanaceae. Fungal Diversity 93: 1–160. https://doi.org/10.1007/s13225-018-0408-6

  67. Tsui, C.K.M., Sivichai, S. & Berbee, M.L. (2006) Molecular systematics of Helicoma, Helicomyces and Helicosporium and their teleomorphs inferred from rDNA sequences. Mycologia 98: 94–104. https://doi.org/10.3852/mycologia.98.1.94

  68. Tsui, C.K.M., Sivichai, S., Rossman, A.Y. & Berbee, M.L. (2007) Tubeufia asiana, the teleomorph of Aquaphila albicans in the Tubeufiaceae, Pleosporales, based on cultural and molecular data. Mycologia 99: 884–894.  https://doi.org/10.1080/15572536.2007.11832520

  69. Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246.

  70. Wu, F., Zhou, L.W. & Dai, Y.C. (2016) Neomensularia duplicata gen. et sp. nov. (Hymenochaetales, Basidiomycota) and two new combinations. Mycologia 108, 891–898.

  71. Yoshimura, S., Zenkoh, T., Ohtsu, Y., Kanasaki, R., Shigematsu, N., Takase, S. & Neya, M. (2003) Isolation, structure determination and biological study of novel gluconeogenesis inhibitors, FR225659 family. Symposium on The Chemistry of Natural Products 45: 281–286.  https://doi.org/10.24496/tennenyuki.45.0_281

  72. Zhao, G.Z., Liu, X. & Wu, W. (2007) Helicosporous hyphomycetes from China. Fungal Diversity 26: 313–524.

  73. Zhou, L.W., Vlasák, J., Qin, W.M. & Dai, Y.C. (2016a) Global diversity and phylogeny of the Phellinus igniarius complex (Hymenochaetales, Basidiomycota) with the description of five new species. Mycologia 108: 192–204.

  74. Zhou, L.W., Vlasák, J. & Dai, Y.C. (2016b) Taxonomy and phylogeny of Phellinidium (Hymenochaetales, Basidiomycota): A redefinition and the segregation of Coniferiporia gen. nov. for forest pathogens. Fungal Biology 120: 988–1001.

  75. Zenkoh, T., Ohtu, Y., Yoshimura, S., Shigematsu, N., Takase, S. & Hino, M. (2003) The novel gluconeogenesis inhibitors FR225659 and FR225656 from Helicomyces sp. No. 19353. III. Structure determination. The Journal of Antibiotics 56 (8): 694–699.  https://doi.org/10.1002/chin.200405155