Skip to main content Skip to main navigation menu Skip to site footer
Article
Published: 2022-01-13

Aspergillus sibiricus (Aspergillaceae, Eurotiales), a novel acid-tolerant species in Aspergillus section Fumigati

Komarov Botanical Institute of the Russian Academy of Sciences, St. Petersburg, Russia
Aspergillus acid tolerant fungi coal mine section Fumigati series Unilaterales soil fungi Fungi

Abstract

A novel species in Aspergillus section Fumigati is described as Aspergillus sibiricus. The new species was isolated from an open coal mine in Altai, Siberia, Russia and is represented by ex-type strain CBS 143307. We used a polyphasic taxonomic approach to characterise the species. A description based on morphological features is provided and shows that the new species differs morphologically from closely related species (A. assulatus, A. nishimurae and A. waksmanii). Partial sequences of the loci encoding β-tubulin (BenA), calmodulin (CaM), ribosomal polymerase II second largest subunit (RPB2) and internal transcribed spacer rDNA region (ITS1-5.8S-ITS2) were analyzed as well. Sequences data, macro- and micromorphological and physiological characteristics distinguish A. sibiricus from all known species in section Fumigati, series Unilaterales. A. sibiricus, isolated from an acidic habitat (pH 3.2–3.4), is able to grow at pH 2 and is considered an acid-resistant species.

References

  1. Aguilera, A. (2013) Eukaryotic organisms in extreme acidic environments: the Río Tinto case. Life 3: 363–374.  https://doi.org/10.3390/life3030363

  2. Ajello, L. (1968) A taxonomic review of the dermatophytes and related species. Sabouraudia 6: 147–149.

  3. Alcazar-Fuoli, L., Mellado, E., Alastruey-Izquierdo, A., Cuenca-Estrella, M. & Rodriguez-Tudela, J.L. (2008) Aspergillus section Fumigati: antifungal susceptibility patterns and sequence-based identification. Antimicrobial Agents and Chemotherapy 52: 1244–1251.  https://doi.org/10.1128/AAC.00942-07

  4. Amaral Zettler, L.A., Gómez, F., Zettler, E., Keenan, B.G., Amils, R. & Sogin, M.L. (2002) Microbiology: eukaryotic diversity in Spain’s river of fire. Nature 417: 137.

  5. Baker, B.J., Lutz, M.A., Dawson, S.C., Bond, P.L. & Banfield, J.F. (2004) Metabolically active eukaryotic communities in extremely acidic mine drainage. Applied and Environmental Microbiology 70: 6264–6271.  https://doi.org/10.1128/AEM.70.10.6264-6271.2004

  6. Balajee, S.A., Nickle, D., Varga, J. & Marr, K.A. (2006) Molecular studies reveal frequent misidentification of Aspergillus fumigatus by morphotyping. Eukaryotic Cell 5: 1705–1712.  https://doi.org/10.1128/EC.00162-06

  7. Banks, D., Burke, S.P. & Gray, C.G. (1997) Hydrogeochemistry of coal mine drainage and other ferruginous waters in north Derbyshire and south Yorkshire, UK. Quarterly Journal of Engineering Geology & Hydrogeology 30: 257–280.  http://dx.doi.org/10.1144/GSL.QJEG.1997.030.P3.07

  8. Berkeley, M.J. (1860) Outlines of British Fungology. 442 pp.

  9. Fresenius, G. (1863) Beiträge zur Mykologie 3.

  10. Glass, N.L. & Donaldson, G.C. (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology 61: 1323–1330.

  11. Gross, S. & Robbins, E.I. (2000) Acidophilic and acid-tolerant fungi and yeasts. Hydrobiologia 433: 91–109.  https://doi.org/10.1023/A:1004014603333

  12. Hong, S.-B., Go, S.-J., Shin, H.-D., Frisvad, J.C. & Samson, R.A. (2005) Polyphasic taxonomy of Aspergillus fumigatus and related species. Mycologia 97: 1316–1329.

  13. Hong, S.-B., Shin, H.-D., Hong, J., Frisvad, J.C., Nielsen, P.V., Varga, J. & Samson, R.A. (2008) New taxa of Neosartorya and Aspergillus in Aspergillus section Fumigati. Antonie van Leeuwenhoek 93: 87–98.  https://doi.org/10.1007/s10482-007-9183-1

  14. Houbraken, J. & Samson, R.A. (2011) Phylogeny of Penicillium and the segregation of Trichocomaceae into three families. Studies in Mycology 70: 1–51. https://doi.org/10.3114/sim.2011.70.01

  15. Houbraken, J., Weig, M., Gro, U., Meijer, M. & Bader, O. (2016) Aspergillus oerlinghausenensis, a new mould species closely related to A. fumigatus. FEMS Microbiology Letters 363: fnv236.  https://doi.org/10.1093/femsle/fnv236

  16. Houbraken, J., Kocsubé, S., Visagie, C.M., Yilmaz, N. Wang, X.-C., Meijer, M., Kraak, B., Hubka, V., Bensch, K., Samson, R.A. & Frisvad, J.C. (2020) Classification of Aspergillus, Penicillium, Talaromyces and related genera (Eurotiales): an overview of families, genera, subgenera, sections, series and species. Studies in Mycology 95: 5–169. https://doi.org/10.1016/j.simyco.2020.05.002

  17. Hubka, V., Peterson, S.W., Frisvad, J.C., Yaguchi, T., Kubatova, A. & Kolarýk, M. (2013) Aspergillus waksmanii sp. nov. and Aspergillus marvanovae sp. nov., two closely related species in section Fumigati. International Journal of Systematic and Evolutionary Microbiology 63: 783–789.  https://doi.org/10.1099/ijs.0.047076-0

  18. Hubka, V., Dudová, Z., Kubátová, A., Frisvad, J.C., Yaguchi, T., Horie, Y., Jurjević, Ž., Hong, S.-B. & Kolařík, M. (2017) Taxonomic novelties in Aspergillus section Fumigati: A. tasmanicus sp. nov., induction of sexual state in A. turcosus and overview of related species. Plant Systematics and Evolution 303: 787–806.  https://doi.org/10.1007/s00606-017-1400-4

  19. Hujslová, M., Kubátová, A., Kostovčík, M. & Kolařík, M. (2012) Acidiella bohemica gen. et sp. nov. and Acidomyces spp. (Teratosphaeriaceae), the indigenous inhabitants of extremely acidic soils in Europe. Fungal Diversity 58: 33–45.  http://dx.doi.org/10.1007/s13225-012-0176-7

  20. Hujslová, M., Kubátová, A., Kostovčík, M., Blanchette, R.A., de Beer, Z.W., Chudíčková, M. & Kolařík, M. (2014) Three new genera of fungi from extremely acidic soils. Mycological Progress 13: 819–831.  http://dx.doi.org/10.1007/s11557-014-0965-3

  21. Hujslová, M., Alena, K., Petra, B., Milada, C. & Miroslav, K. (2017) Extremely acidic soils are dominated by species-poor and highly specific fungal communities. Microbial Ecology 73: 321–337.  http://dx.doi.org/10.1007/s00248-016-0860-3

  22. Kelly, K.L. (1964) Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Government Printing Office, Washington, DC: US.

  23. Kolařík, M., Hujslová, M. & Vazquéz-Campos, X. (2015) Acidotolerant genus Fodinomyces (Ascomycota: Capnodiales) is a synonym of Acidiella. Czech Mycology 67: 37–38.

  24. Kontoyiannis, D.P. & Bodey, G.P. (2002) Invasive aspergillosis in 2002: an update. European Journal of Clinical Microbiology & Infectious Diseases 21: 161–172.  https://doi.org/10.1007/s10096-002-0699-z

  25. Kumar, S., Stecher, G., Li, M., Knyaz, C. & Tamura, K. (2018) MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution 35: 1547–1549. https://doi.org/10.1093/molbev/msy096

  26. Larkin, M.A., Blackshields, G., Brown, N.P., Chenna, R., McGettigan, P.A., McWilliam, H., Valentin, F., Wallace, I.M., Wilm, A., Lopez, R., Thompson, J.D., Gibson, T.J. & Higgins, D.G. (2007) Clustal W and Clustal X version 2.0. Bioinformatics 23: 2947–2948.

  27. Matsuzawa, T., Takaki, G.M.C., Yaguchi, T., Okada, K., Gonoi, T. & Horie, Y. (2014) Two new species of Aspergillus section Fumigati isolated from caatinga soil in the state of Pernambuco, Brazil. Mycoscience 55: 79–88.  https://doi.org/10.1016/j.myc.2013.04.001

  28. McLennan, E.I., Ducker, S.C. & Thrower, L.B. (1954) New soil fungi from Australian heathland: Aspergillus, Penicillium, Spegazzinia. Australian Journal of Botany 2 (3): 355–364.

  29. McNeill, J., Barrie, F.R., Buck, W.R., Demoulin, V., Greuter, W., Hawksworth, D., Herendeen, P.S., Knapp, S., Marhold, K., Prado, J., Prud’Homme van Reine, W.F., Smith, G.F., Wiersema, J.H. & Turland, N.J. (2012) International Code of Nomenclature for algae, fungi, and plants (Melbourne Code). Koeltz Scientific Books, Königstein, 208 pp.

  30. Nováková, A., Hubka, V., Saiz-Jimenez, C. & Kolarik, M. (2012) Aspergillus baeticus sp. nov. and Aspergillus thesauricus sp. nov., two species in section Usti from Spanish caves. International Journal of Systematic and Evolutionary Microbiology 62: 2778–2785.  https://doi.org/10.1099/ijs.0.041004-0

  31. Qureshi, A., Maurice, C. & Öhlander, B. (2016) Potential of coal mine waste rock for generating acid mine drainage. Journal of Geochemical Exploration 160: 44–54. https://doi.org/10.1016/j.gexplo.2015.10.014

  32. Raper, K.B. & Fennell, D.I. (1965) The genus Aspergillus. Williams & Wilkins, Baltimore, 686 pp.

  33. Saluja, P. & Prasad, G.S. (2007) Debaryomyces singareniensis sp. nov., a novel yeast species isolated from a coalmine soil in India. FEMS Yeast Research 7: 482–488. https://doi.org/10.1111/j.1567-1364.2006.00182.x

  34. Saluja, P., Yelchuri, R.K., Sohal, S.K., Bhagat, G., Paramjit & Prasad, G.S. (2012) Torulaspora indica a novel yeast species isolated from coal mine soils. Antonie van Leeuwenhoek 101: 733–742.  https://doi.org/10.1007/s10482-011-9687-6

  35. Samson, R.A., Hong, S.-B., Peterson, S.W., Frisvad, J.C. & Varga, J. (2007) Polyphasic taxonomy of Aspergillus section Fumigati and its teleomorph Neosartorya. Studies in Mycology 59: 147–207.  https://doi.org/10.3114/sim.2007.59.14

  36. Samson, R.A., Houbraken, J., Thrane, U., Frisvad, J.C. & Andersen, B. (2010) Food and indoor fungi, CBS Laboratory Manual Series 2. CBS-Fungal Biodiversity Centre, Utrecht, 390 pp.

  37. Samson, R.A., Visagie, C.M., Houbraken, J., Hong, S.B., Hubka, V., Klaassen, C.H.W., Perrone, G., Seifert, K.A., Susca, A., Tanney, J.B., Varga, J., Kocsubé, S., Szigeti, G., Yaguchi, T. & Frisvad, J.C. (2014) Phylogeny, identification and nomenclature of the genus Aspergillus. Studies in Mycology 78: 141–173. https://doi.org/10.1016/j.simyco.2014.07.004

  38. Schoch, C.L., Seifert, K.A., Huhndorf, S., Robert, V., Spogue, J.L., Levesque, C.A. & Chen, W. (2012) Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi. Proceedings of the National Academy of Sciences 109: 6241–6246.  https://doi.org/10.1073/pnas.1117018109

  39. Selbmann, L., de Hoog, G.S., Zucconi, L., Isola, D., Ruisi, S., Gerrits, A.H.G., Ende, V.A., Ruibal, C., De Leo, F., Urzì, C. & Onofri, S. (2008) Drought meets acid: three new genera in a dothidealean clade of extremotolerant fungi. Studies in Mycology 61: 1–20. https://doi.org/10.3114/sim.2008.61.01

  40. Sugui, J.A., Peterson, S.W., Figat, A., Hansen, B., Samson, R.A., Mellado, E., Cuenca-Estrella, M. & Kwon-Chung, K.J. (2014) Genetic relatedness versus biological compatibility between Aspergillus fumigatus and related species. Journal of Clinical Microbiology 52: 3707–3721. https://doi.org/10.1128/JCM.01704-14

  41. Takada, M., Horie, Y. & Abliz, P. (2001) Two new heterothallic Neosartorya from African soil. Mycoscience 42: 361–367

  42. Tamura, K. & Nei, M. (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution 10: 512–526.  https://doi.org/10.1093/oxfordjournals.molbev.a040023

  43. Udagawa, S., Tsubouchi, H. & Horie, Y. (1991) Neosartorya hiratsukae, a new species of food-borne Ascomycetes. Transactions of the Mycological Society of Japan 32: 23–29.

  44. Ulfig, K. & Korcz, M. (1995) Isolation of keratinolytic fungi from a coal mine dump. Mycopathologia 129: 83–86.

  45. White, T.J., Bruns, T.D., Lee, S.B. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, T.J. (Eds.) PCR protocols: a guide to methods and applications. Academic Press, London, UK, pp. 315–322.

  46. Younger, P.L. (2004) Environmental impacts of coal mining and associated wastes: a geochemical perspective. Geological Society London Special Publications 236: 169–209. https://doi.org/10.1144/GSL.SP.2004.236.01.12

  47. Zak, J.C. & Wildman, H.G. (2004) Fungi in stressful environments. In: Mueller, G.M., Bills, G.F. & Foster, M.S. (Eds.) Biodiversity of fungi, inventory and monitoring methods. Elsevier Academic Press, Amsterdam etc., pp. 303–331.

  48. Zirnstein, I., Arnold, T., Krawczyk-Bärsch, E., Jenk, U., Bernhard, G. & Röske, I. (2012) Eukaryotic life in biofilms formed in a uranium mine. The Open Microbiology Journal 1: 83–94.  https://doi.org/10.1002/mbo3.17