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Article
Published: 2022-08-04

Morphological and molecular notes of Gypsophila pilulifera

Department of Biology, Faculty of Science, Akdeniz University, Antalya, Turkey
Department of Biology, Faculty of Science, Akdeniz University, Antalya, Turkey
Department of Biology, Faculty of Science, Akdeniz University, Antalya, Turkey
Department of Molecular Biology and Genetic, Faculty of Science, Muğla Sıtkı Koçman University, Muğla, Turkey
Department of Molecular Biology and Genetic, Faculty of Science, Muğla Sıtkı Koçman University, Muğla, Turkey
Gypsophila molecular analyses morphology statistical analyses Eudicots

Abstract

A detailed study of the Turkish endemic Gypsophila pilulifera were presented by both morphological and molecular investigations of several sites (each one divided in four biotopes). The characteristics were investigated using statistic analyses, i.e. t-Test and One-way ANOVA tests, as well as Discriminant analysis (á=0.05). Some differences were detected among the individuals in the locations stufied where the species was distributed in terms of morphological characteristics and obvious similarities/differences were determined among the biotopes as a result of the discriminant analysis. Morphological characterization was supported by molecular data. Four individuals from two different locations were analyzed by PCR using twenty-four ISSR primers. Analysis of PCR products on agarose gel yielded three primary reproductive band profiles, and the similarity frequencies calculated with the Jaccard coefficient were found to be among 0.229 and 0.769. In this context, according to the results, there was no difference in species, subspecies or variety level between individuals between the two locations. But the obtained molecular differences support the morphological differences. It is predicted that this may change towards speciation as ecological changes increase in the locations where the species is distributed.

References

  1. Ángeles Alonso, M., Crespo, M.B., Martinez-Azorin, M. & Mucina, L. (2021) Morphological and molecular data support recognition of Spergularia quartzicola (Caryophyllaceae ) as a new species endemic to South Africa. Plant Biosystems 156 (2): 506–514.  https://doi.org/10.1080/11263504.2021.1887955

  2. Arslan, I., Çelik, A. & Chol, J.H.A. (2012) Cytotoxic triterpenoid saponin from under-ground parts of Gypsophila pilulifera Boiss. & Heldr. Fitoterapia 83: 699–703.  https://doi.org/10.1016/j.fitote.2012.02.005

  3. Ataşlar, E. (2000) Gypsophila L. In: Güner, A., Özhatay, N., Ekim, T. & Başer, K.H.C. (Eds.) Flora of Turkey and the East Aegean Islands, vol. 11 (Supplement). Edinburgh Univ. Press, Edinburgh, pp. 49–50.

  4. Barkoudah, Y.I. (1962) A Revision of Gypsophila, Bolanthus, Ankyropetalum and Phryna. Wentia, Amsterdam, North-Holland, 203 pp.  https://doi.org/10.1111/j.1438-8677.1962.tb00012.x

  5. Boissier, E. (1842) Diagnoses Plantarum Orientalium Novarum, vol. 2(8). A.Ascher & Co., Leipzig, 128 pp.

  6. Boissier, E. (1867) Flora Orientalis: sive Enumeratio Plantarum in Oriente a Graecia et Aegypto ad Indiae fines hucusque observatarum, vol. 1. Primum, A.Ascher & Co., Genevӕ, 548 pp.  https://doi.org/10.5962/bhl.title.20323

  7. Cain, M., Milligan, B. & Strand, A. (2000) Long-distance seed dispersal in plant populations. American Journal of Botany 87: 1217–1227.  https://doi.org/10.2307/2656714

  8. Chima, N.K., Nahar, L., Majinda, R.R.T., Çelik, S. & Sarker, S.D. (2014) Assessment of free-radical scavenging activity of Gypsophila pilulifera: Assay-Guided isolation of verbascoside as the main active component. Revista Brasileira de Farmacognosia 24 (1): 38–43.  https://doi.org/10.1590/0102-695X20142413391

  9. Davis, P.H. (1965) Flora of Turkey and the East Aegean Islands, vol. 1. Edinburgh University Press, Edinburgh, 567 pp.

  10. Davis, P.H., Mill, R.R. & Tan, K. (Eds.) (1988) Gypsophila L. Flora of Turkey and the East Aegean Islands, vol. 10 (Supplement). Edinburgh University Press, Edinburgh, pp. 73–75.

  11. Dittbrenner, A., Hensen, I. & Wesche, K. (2005) Genetic structure and random amplified polymorphic DNA diversity of the rapidly declining Angelica palustris (Apiaceae) in Eastern Germany in relation to population size and seed production. Plant Species Biology 20: 191–200.  https://doi.org/10.1111/j.1442-1984.2005.00138.x

  12. Dole, J.M. & Wilkins, H.F. (2005) Floriculture: Principles and species. 2nd Edition. Pearson/Prentice Hall, Upper Saddle River, 1023 pp.

  13. Doyle, J.J. & Doyle, J.L. (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin 19: 11–15.

  14. Düşen, O., Göktürk, R.S., Kaya, E., Sarpkaya, U. & Gürcan, B. (2018) Morphological and molecular determination of a new Viola species (Violaceae) from Turkey. Phytotaxa 369 (1): 37–46.  https://doi.org/10.11646/phytotaxa.369.1.4

  15. Ekim, T., Koyuncu, M., Vural, M., Duman, H., Aytaç, Z. & Adıgüzel, N. (2000) Red Data Book of Turkish Plants. Barışcan Press, Ankara, 246 pp.

  16. Göktürk, R.S., Düşen, O., Kaya, E., Gürcan, B. & Sarpkaya, U. (2019) A new variety of Plocama calabrica (Rubiaceae) from Denizli (Turkey) confirmed by morphological and molecular ISSR markers. Acta Botanica Croatica 78 (2): 142–146.  https://doi.org/10.2478/botcro-2019-0016

  17. Hernández-Ledesma, P., Berendsohn, W.G., Borsch, T., Mering, S.V., Akhani, H., Arias, S., CastañedaNoa, I., Eggli, U., Eriksson, R., Flores-Olvera, H., Fuentes-Bazán, S., Kadereit, G., Klak, C., Korotkova, N., Nyffeler, R., Ocampo, G., Ochoterena, H., Oxelman, B., Rabeler, R.K., Sanchez, A., Schlumpberger, B.O. & Uotila, P. (2015) A taxonomic backbone for the global synthesis of species diversity in the angiosperm order Caryophyllales. Willdenowia 45: 281–383.  https://doi.org/10.3372/wi.45.45301

  18. Huber-Morath, A. (1967) Gypsophila L. In: Davis, P.H. (Ed.) Flora of Turkey and the East Aegean Islands, vol. 2. Edinburgh University Press, Edinburgh, pp. 149–171.

  19. IUCN Standards and Petitions Committee (2019) Guidelines for Using the IUCN Red List Categories and Criteria. Version 14. Prepared by the Standards and Petitions Committee. Gland, Switzerland and Cambridge, UK,16 pp.

  20. Kaya, E. (2015) ISSR Analysis for determination of genetic diversity and relationship in some Turkish olive (Olea europaea L.) Cultivars. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 43 (1): 96–99.  https://doi.org/10.15835/nbha4319818

  21. Kaya, E. & Souza, F.V.D. (2017) Comparison of two PVS2-based procedures for cryopreservation of commercial sugarcane (Saccharum spp.) germplasm and confirmation of genetic stability after cryopreservation using ISSR markers. In Vitro Cellular and Developmental Biology – Plant 53: 410–417.  https://doi.org/10.1007/s11627-017-9837-2

  22. Kaya, E. & Yılmaz Gökdoğan, E. (2016) Using two retrotransposon based marker systems (IRAP and REMAP) for Molecular Characterization of olive (Olea europaea L.) cultivars. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 44 (1): 167–174.  https://doi.org/10.15835/nbha44110158

  23. Kołodziej, B., Okoñ, S., Nucia, A., Ociepa, T., Luchowska, K., Sugier, D., Gevrenova, R. & Henry, M. (2018) Morphological, chemical, and genetic diversity of Gypsophila L. (Caryophyllaceae) species and their potential use in the pharmaceutical industry. Turkish Journal of Botany 42 (3): 257–270.  https://doi.org/10.3906/bot-1707-13

  24. Korkmaz, M. (2007) Türkiye’de yetişen tek yıllık Gypsophila L. (Caryophyllaceae) taksonları üzerinde biyosistematik çalışmalar (in Turkish). PhD thesis. Süleyman Demirel University, Isparta, 260 pp.

  25. Korkmaz, M., Özçelik, H. & İlhan, V. (2012) Türkiye’nin bazı Gypsophila L. (Caryophyllaceae) taksonlarının habitat özellikleri (in Turkish). Biyoloji Bilimleri Araştırma Dergisi 5 (2): 111–125.

  26. Linnaeus, C. (1753) Species Plantarum, vol. 1. Laurentii Salvii, Stockholm, 560 pp.

  27. Mabberley, D.J. (2018) Mabberley’s Plant-Book: A portable dictionary of plants, their classification and uses, fourth edition. Cambridge University Press, UK, 1102 pp.  https://doi.org/10.1017/9781316335581

  28. Markus, S.D. & Joachim W.K. (2022) The distinction between Sagina apetala and S. micropetala (Caryophyllaceae: Sagineae), their phylogenetic relationships, and a note on the coastal origin of some widespread ruderals. Willdenowia 52 (1): 5–23.  https://doi.org/10.3372/wi.52.52101

  29. Muca, B. (2017) Türkiye Gypsophila L. (Caryophyllaceae) cinsine ait Capituliformes Williams seksiyonunun revizyonu (in Turkish). PhD thesis. Süleyman Demirel University, Isparta, 338 pp.

  30. Özbek Yazıcı, S. & Özmen, İ. (2018) Effect of the crude saponin extract from Gypsophila pilulifera Boiss. & Heldr. on protease from Bacillus subtilis ATCC 6633 and antioxidant properties of the extract. Iranian Journal of Science and Technology 42 (4): 1707–1713.  https://doi.org/10.1007/s40995-017-0366-y

  31. Özçelik, H. & Muca, B. (2010) Ankyropetalum Fenzl (Caryophyllaceae) cinsine ait türlerin Türkiye’deki yayılışı ve habitat özellikleri (in Turkish). BIBAD (Research Journal of Biology Sciences) 3 (2): 47–56.

  32. Özçelik, H. & Özgökçe, F. (2021) Taxonomic contributions to genus Gypsophila in Turkey and a new taxon from Erzurum: G. venusta subsp. staminea. Anatolian Journal of Botany 5 (1): 6–18.  https://doi.org/10.30616/ajb.791872

  33. Özhatay, N. & Kültür, Ş. (2006) Check-list of additional taxa to the supplement Flora of Turkey III. Turkish Journal of Botany 30: 281–316.

  34. Özüdoğru, E.A., Kaya, E., Kırdök, E. & İşsever-Öztürk, S. (2011) In vitro propagation from young and mature explants of thyme (Thymus vulgaris and Thymus longicaulis) resulting in genetically stable shoots. In Vitro Cell Dev Biol-Plant 47: 309–320.  https://doi.org/10.1007/s11627-011-9347-6

  35. POWO (2020) International Plant Names Index, Available from: https://powo.science.kew.org/results?q=Gypsophila%20pilulifera (accessed 1 January 2022)

  36. Rohlf, F.J. (1998) NTSYS-pc numerical taxonomy and multivariate analysis system version 2.0. Exeter Publications, New York, 43 pp.

  37. Stenoien, H., Fenster, C., Tonteri, A. & Savolainen, O. (2005) Genetic variability in natural populations of Arabidopsis thaliana in northern Europe. Molecular Ecology 14: 137–148.  https://doi.org/10.1111/j.1365-294X.2004.02359.x

  38. Sumaira, S., Mir, A.K., Akbar, A.M. & Asma, J. (2008) Polen Morphology of the Genus Silene (Silene-Caryopyllaceae) from Pakistan. Biological Diversity and Conservation (BioDiCon) 1 (2): 74–85.

  39. Washitani, I., Ishikama, F., Matsumura, C., Nagal, M. & Nishihiro, M.A. (2005) Conservation ecology of Primula sieboldii: syntesisis of information toward the prediction of the genetic /demographic fate of a population. Plant Species Biology 20: 3–15.  https://doi.org/10.1111/j.1442-1984.2005.00127.x

  40. Williams, F.N. (1989) Revision of the forms of the genus Gypsophila L. Jown Botanica of London 27: 321–329.

  41. Zhang, M.L., Zeng, X.Q., Li, C., Sanderson, S.C., Byalt, V.V. & Lei, Y. (2017) Molecular phylogenetic analysis and character evolution in Pseudostellaria (Caryophyllaceae) and description of a new genus, Hartmaniella, in North America, Botanical Journal of the Linnean Society 184 (4): 444–456.  https://doi.org/10.1093/botlinnean/box036