Two novel Oidiodendron species isolated from roots of Vaccinium boninense in the Bonin Islands, Japan

DAI HIROSE; TOSHIYUKI TOKIWA; TAKASHI YAGUCHI

doi:10.11646/phytotaxa.566.1.5

Issue: Vol. 566 No. 1: 22 September 2022

Type: Article

Published: 2022-09-22

DOI: 10.11646/phytotaxa.566.1.5

Page range: 89-104

Abstract views: 118

PDF downloaded: 6

**Two novel Oidiodendron species isolated from roots of Vaccinium boninense in the Bonin Islands, Japan**

DAI HIROSE⁺⁻
TOSHIYUKI TOKIWA⁺⁻
TAKASHI YAGUCHI⁺⁻

School of Pharmacy, Nihon University, 7–7–1 Narashinodai, Funabashi, Chiba 274-8555, Japan

Medical Mycology Research Center, Chiba University, 1‒8‒1 Inohana, Chuo, Chiba, 260-8673 Japan

ericoid mycorrhiza Helotiales morphology Myxotrichaceae phylogeny Fungi

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Abstract

Four strains of the fungal genus Oidiodendron were isolated from the fine roots of Vaccinium boniense which is distributed only on the Bonin Islands, Japan. Two new species, Oidiodendron tokumasui (NBRC 115411^T) and Oidiodendron boninense (NBRC 115392^T), are described in this paper. Molecular phylogenetic analyses based on multigene sequence-data showed that each novel species formed a unique clade. Oidiodendron tokumasui is phylogenetically related to O. maius but can be distinguished from O. maius by good growth at 25‒30 ˚C and pH 7‒9, colony diameter after 28 days, and length of the conidiophores. Oidiodendron boninense is phylogenetically related to O. setiferum, O. rammosum, O. muniellense, and O. fuscum but differs from those four species in the following aspects: good growth at 25 ˚C and pH 7, absence of melanized appendages, spinulate and thick-walled conidia, and length of the conidiophores. Both new species formed ericoid mycorrhizal-like hyphal coils in the rhizodermal cells of sterile blueberry seedlings in vitro.

References

Barron, G.L. (1962) New species and new records of Oidiodendron. Canadian Journal of Botany 40: 589–607. 
Baba, T. & Hirose, D. (2020) Morphological characteristics of rhizodermal colonization by Leohumicola species in an ericaceous host. Plant Roots 14: 1–10. https://doi.org/10.3117/plantroot.14.1
Baba, T. & Hirose, D. (2021) Slow-growing fungi belonging to the unnamed lineage in Chaetothyriomycetidae form hyphal coils in vital ericaceous rhizodermal cells in vitro. Fungal Biology 125: 1026–1035. https://doi.org/10.1016/j.funbio.2021.07.003
Baba, T., Hirose, D., Noma, S. & Ban, T. (2021) Inoculation with two Oidiodendron maius strains differentially alters the morphological characteristics of fibrous and pioneer roots of Vaccinium virgatum ‘Tifblue’ cuttings. Scientia Horticulturae 281: 109948. https://doi.org/10.1016/j.scienta.2021.109948
Couture, M., Fortin, J. & Dalpé, Y. (1983) Oidiodendron griseum Robak: an endophyte of ericoid mycorrhiza in Vaccinium spp. New Phytologist 95: 375–380.  https://doi.org/10.1111/j.1469-8137.1983.tb03505.x
Crous, P.W., Wingfield, M.J., Burgess, T.I., Hardy, G.E.S.J., Gené, J., Guarro, J., Baseia, I.G., Garcia, D., Gusmão, L.F.P., Souza-Motta, C.M., Thangavel, R., Adamik, S., Barili, A., Barnes, C.W., Bezerra, J.D.P., Bordallo, J.J., Cano-Lira, J.F., de Oliveira, R.J.V., Ercole, E., ... & Groenewald, J.Z. (2018) Fungal Planet description sheets: 716–784. Persoonia 40: 240–393. https://doi.org/10.3767/persoonia.2018.40.10
Currah, R., Tsuneda, A. & Murakami, S. (1993) Conidiogenesis in Oidiodendron periconioides and ultrastructure of ericoid mycorrhizas formed with Rhododendron brachycarpum. Canadian Journal of Botany 71: 1481–1485.  https://doi.org/10.1139/b93-179
Dalpé, Y. (1986) Axenic synthesis of ericoid mycorrhiza in Vaccinium angustifolium Ait. by Oidiodendron species. New Phytologist 103: 391–396.  https://doi.org/10.1111/j.1469-8137.1986.tb00624.x
Dalpé, Y. (1991) Statut endomycorhizien du genre Oidiodendron. Canadian Journal of Botany 69: 1712–1714.  https://doi.org/10.1139/b91-217
Darriba, D., Posada, D., Kozlov, A.M., Stamatakis, A., Morel, B. & Flouri, T. (2020) ModelTest-NG: A new and scalable tool for the selection of DNA and protein evolutionary models. Molecular Biology and Evolution 37: 291–294. https://doi.org/10.1093/molbev/msz189
Domsch, K.H., Grams, W. & Anderson, T.H. (2007) Compendium of soil fungi second edition. IHW-Verlag, Eching.
Douglas, G., Heslin, M. & Reid, C. (1989) Isolation of Oidiodendron maius from Rhododendron and ultrastructural characterization of synthesized mycorrhizas. Canadian Journal of Botany 67: 2206–2212.  https://doi.org/10.1139/b89-280
Edler, D., Klein, J., Antonelli, A. & Silvestro, D. (2021) raxmlGUI 2.0: A graphical interface and toolkit for phylogenetic analyses using RAxML. Methods in Ecology and Evolution 12: 373–377. http://dx.doi.org/10.1111/2041-210X.13512
Hambleton, S., Egger, K. & Currah, R. (1998) The genus Oidiodendron: species delimitation and phylogenetic relationships based on nuclear ribosomal DNA Analysis. Mycologia 90: 854–68. https://doi.org/10.2307/3761327
Hiradate, S. (2019) Properties of soils of the Ogasawara Islands: keys to understand past nature and find adequate management for future. Global Environmental Research 23: 29–36.
Johnston, P.R., Quijada, L., Smith, C.A., Baral, H.O., Hosoya, T., Baschien, C., Pärtel, K., Zhuang, W.Y., Haelewaters, D., Park, D., Carl, S., López-Giráldez, F., Wang, Z. & Townsend, J.P. (2019) A multigene phylogeny toward a new phylogenetic classification of Leotiomycetes. IMA Fungus 10: 1. https://doi.org/10.1186/s43008-019-0002-x
Katoh, K., Rozewicki, J. & Yamada, K.D. (2019) MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20: 1160–1166. https://doi.org/10.1093/bib/bbx108
Kobae, Y. & Ohtomo, R. (2016) An improved method for bright-field imaging of arbuscular mycorrhizal fungi in plant roots. Soil Science and Plant Nutrition 62: 27–30. https://doi.org/10.1080/00380768.2015.1106923
Kozlov, A.M., Darriba, D., Flouri, T., Morel, B. & Stamatakis, A. (2019) RAxML-NG: a fast, scalable and user-friendly tool for maximum likelihood phylogenetic inference. Bioinformatics 35: 4453–4455. https://doi.org/10.1093/bioinformatics/btz305
Lemoine, F., Domelevo Entfellner, J.B., Wilkinson, E., Correia, D., Dávila Felipe, M., De Oliveira, T. & Gascuel, O. (2018) Renewing Felsenstein’s phylogenetic bootstrap in the era of big data. Nature 556: 452–456. https://doi.org/10.1038/s41586-018-0043-0
Leopold, D.R. (2016) Ericoid fungal diversity: challenges and opportunities for mycorrhizal research. Fungal Ecology 24: 114–123. https://doi.org/10.1016/j. funeco.2016.07.004
Morita, S., Kato, H., Iwasaki, N., Kusumoto, Y., Yoshida, K. & Hiradate, S. (2010) Unusually high levels of bio-available phosphate in the soils of Ogasawara Islands, Japan: Putative influence of seabirds. Geoderma 160: 155–164. https://doi.org/10.1016/j.geoderma.2010.09.008
Murray, M.G. & Thompson, W.F. (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research 8: 4321–4326. https://doi.org/10.1093/nar/8.19.4321
O’Donnell, K. & Cigelink, E. (1997) Two divergent intragenomic rRNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Molecular Phylogenetics and Evolution 7: 103–116. https://doi.org/10.1006/mpev.1996.0376
Ogawa, Y., Suda, A., Kusama-Eguchi, K., Watanabe, K. & Tokumasu, S. (2005) Intraspecific groups of Umbelopsis ramanniana inferred from nucleotide sequences of nuclear rDNA internal transcribed spacer regions and sporangiospore morphology. Mycoscience 46: 343–351. https://dx.doi.org/10.1007/s10267-005-0257-5
Raja, H., Schoch, C.L., Hustad, V., Shearer, C. & Miller, A. (2011) Testing the phylogenetic utility of MCM7 in the Ascomycota. MycoKeys 1: 63–94. https://doi.org/10.3897/mycokeys.1.1966
Rayner, R. (1970) A mycological colour chart. Commonwealth Mycological Institute, Surrey & British Mycological Society, Kew.
Rice, A. & Currah, R. (2005) Oidiodendron: A survey of the named species and related anamorphs of Myxotrichum. Studies in Mycology 53: 83–120. https://doi.org/10.3114/sim.53.1.83
Rice, A. & Currah, R. (2006) Oidiodendron maius: saprobe in Sphagnum peat, mutualist in ericaceous roots? In: Schulz, B.J.E., Boyle, C.J.C. & Sieber, T.N. (eds.) Microbial root endophytes. Springer Berlin / Heidelberg, Berlin, pp. 227–246. 
Robak, H. (1932) Investigations regarding fungi on Norwegian ground wood pulp and fungal infections at wood pulp mills. Saertrykk Nyt Mag. for Naturvidenskaberne 71: 185–330.
Rodríguez-Andrade, E., Stchigel, A.M., Terrab, A., Guarro, J. & Cano-Lira, J.F. (2019) Diversity of xerotolerant and xerophilic fungi in honey. IMA Fungus 10: 20. https://doi.org/10.1186/s43008-019-0021-7
Sato, T., Uzuhashi, S., Hosoya, T. & Hosaka, K. (2010) A list of fungi found in the Bonin (Ogasawara) Islands. Ogasawara Research 35: 59–160.
Schmitt, I., Crespo, A., Divakar, P.K., Fankhauser, J.D., Herman-Sackett, E., Kalb, K., Nelsen, M.P., Nelson, N.A., Rivas-Plata, E., Shimp, A.D., Widhelm, T. & Lumbsch, H.T. (2009) New primers for promising single-copy genes in fungal phylogenetics and systematics. Persoonia 23: 35–40. https://doi.org/10.3767/003158509X470602
Seifert, K.A., Hughes, S.J., Boulay, H. & Louis-Seize, G. (2007) Taxonomy, nomenclature and phylogeny of three cladosporium-like hyphomycetes, Sorocybe resinae, Seifertia azaleae and the Hormoconis anamorph of Amorphotheca resinae. Studies in Mycology 58: 235–245. https://doi.org/10.3114/sim.2007.58.09
Tian, W., Zhang, C.Q., Qiao, P. & Milne, R. (2011) Diversity of culturable ericoid mycorrhizal fungi of Rhododendron decorum in Yunnan, China. Mycologia 103: 703–709. https://doi.org/10.3852/10-296
Tokumasu, S. (1973) Notes on Japanese Oidiodendron. Transactions of the Mycological Society of Japan 14: 246–255
Tokumasu, S. (1996) Mycofloral succession on Pinus densiflora needles on a modern site. Mycoscience 37: 313–321. https://doi.org/10.1007/BF02461303
Toyoda, T. (2003) Flora of Bonin Islands, 2nd Ed. Enlarged & Revised, Aboc & Co., Ltd., Kamakura, Japan. [in Japanese]
Vilgalys, R. & Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
Wei, X., Chen, J., Zhang, C. & Pan, D. (2016) A new Oidiodendron maius strain isolated from Rhododendron fortunei and its effects on nitrogen uptake and plant growth. Frontiers in Microbiology 7: 1327. https://doi.org/10.3389/fmicb.2016.01327
White, T.J., Bruns, T., Lee, S. & Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis, M.A., Gelfand, D.H., Sninsky, J.J. & White, Y.J. (eds.) PCR Protocols: A Guide to Methods and Application. Academic Press, San Diego, pp 315–322.
Xiao, G. & Berch, S. (1995) The ability of known ericoid mycorrhizal fungi to form mycorrhizae with Gaultheria shallon. Mycologia 87: 467–70. https://doi.org/10.2307/3760763