Pyemotes zhonghuajia is an ectoparasitoid natural enemy of a wide range of pests such as Lepidoptera, Coleoptera and Hymenoptera. Phthorimaea operculella (Zeller) (PTM) is a worldwide potato pest with serious damage of potato production. Our study showed that one P. zhonghuajia female could kill 1st to 4th instar larvae of PTM, and the lethal time of one P. zhonghuajia female on first to fourth PTM instar larvae were 5.48, 11.33, 23.47 and 2076 min, respectively. The parasitism of 1, 3, 5, 10, and 20 P. zhonghuajia mites could significantly affect the mortality rate of PTM 4th instar larvae and pupae. The parasitism durations (30 and 60 mins) of one P. zhonghuajia female on one PTM fourth instar larva could influence the life history of PTM parental generation but did not influence the life table of PTM offspring generation. Releasing 60, 80, and 100 mites or opisthosoma with mites could reduce the occurrence and consumption of PTM on potatoes the weight loss of the potato tuber. The number of insect dung piles in one potato tuber was significantly decreased by releasing 60, 80, and 100 P. zhonghuajia mites, and releasing 100 mites resulted in the lowest number of insect dung piles. Our results indicate that P. zhonghuajia can significantly affect the population growth of PTM. Future studies should explore the lethal mechanism of P. zhonghuajia and its performance in practical application in the warehouse and field condition.