Abstract
A new species Macrobrachium ramae is described from Rupnarayana river, West Bengal, India along with its molecular characterization and Scanning electron microscopy. The species shares certain characters with M.gurudeve, M.jayasreei, M.kunjuramani and M.saengphani but differs remarkably from these species in the structure and shape of rostrum, telson, appendix masculina and in the size of the proximal segment of the antennular peduncle. Molecular characterization and phylogenetic analysis of M.ramae with mitochondrial COI and 16S rRNA genes reinforce the morphological conclusion and supports the view that it is a new species.
References
Cai, Y. & Vidthayanon, C. (2016) Macrobrachium spelaeus, a new species of stygobitic freshwater prawn from Thailand (Decapoda:Palaemonidae). Raffles Bulletin of Zoology, 64, 117–122.
Chace, F.A. & Bruce, A.J. (1993) The caridean shrimps (Crustacea: Decapoda) of the ‘Albatross’ Philippine Expedition 1907–1910, part 6: Superfamily Palaemonoidea. Smithsonian Contributions to Zoology, 543(543), 1–152.
https://doi.org/10.5479/si.00810282.543
De Grave, S. & Fransen, C.H.J.M. (2011) Carideorum catalogus: the recent species of the dendrobranchiate, stenopodidean, procarididean and caridean shrimps (Crustacea: Decapoda). Zoologische Mededelingen, 85 (9), 195–589.
Holthuis, L.B. (1950a) The decapod of the siboge expedition. Part X. the Palaemonidae collected by the Siboga and Snellius expeditions, with remarks on other species, part I: Sub family Palaemoninae. Siboga Expedition Leiden, 39, 1–268.
Holthuis, L.B. (1950b) Preliminary descriptions of twelve new Species of Palaemonid prawns from American waters (Crustacea: Decapoda). Proceedings van de Koninklijke Nederlandsche Akademie van Wetenschappen, C, 53, 93–99.
Jayachandran, K.V. (2001) Palaemonid Prawns- Biodiversity, Taxonomy, Biology and Management. Oxford and IBH Publ. Company Pvt. Ltd., New Delhi, Calcutta, 624 pp.
Jayachandran, K.V. & Raji, A.V. (2004) Three new species of Macrobrachium Bate, 1868 (Decapoda, Palaemonidae) from the Western Ghats of Kerala State, India. Crustaceana, 77 (10), 1179–1192.
https://doi.org/10.1163/1568540043166100
Jose, D. & Harikrishnan, M. (2019) Evolutionary history of genus Macrobrachium inferred from mitochondrial markers: a molecular clock approach. Mitochondrial DNA Part A 30, 92– 100.
https://doi.org/10.1080/24701394.2018.1462347
Kemp, S. (1917) Leander styliferus Milne Edwards, and related Forms, Notes on Crustacea, Decapoda in the Indian Museum, IX. Records of the Indian Museum, 13, 203–231.
https://doi.org/10.5962/bhl.part.5860
Liu, M.Y., Cai, Y.X. & Tzeng, C.S. (2007) Molecular systematics of the freshwater prawn genus Macrobrachium Bate, 1868 (Crustacea, Decapoda, Palaemonidae) inferred from mtDNA sequences, with emphasis on East Asian species. Zoological Studies, 46 (3), 279–28.
Mandal, A., Varkey, M., Sobhanan, S.P., Mani, A.K., Gopalakrishnan. A., Ganesh, K., Arulraj, S., Pandiarajan, S. & Thampisamraj, Y.C. (2014) Molecular markers reveal only two mud crab species of Genus Scylla (Brachyura: Portunidae) in Indian coastal waters. Biochemical Genetics, 52, 338–354.
https://doi.org/10.1007/s10528-014-9651-z
Pahari, P.R., Das. M. & Bhattacharya, T. (2020) Palaemonid prawns of Purba Medinipur with two new records from West Bengal. Ecology Environment & Conservation, 26 (2), 348–354.
Pillai, P.M. & Unnikrishnan, V. (2012) A new species of Macrobrachium (Decapoda, Palaemonidae) from Vamanapuram River, Southern Kerala, S. India. Zootaxa, 3528 (1), 63–68.
https://doi.org/10.11646/zootaxa.3528.1.5
Pillai, P.M. & Unnikrishnan, V. (2013a) Morphology and molecular phylogeny of Macrobrachium snpurii, a new species of the genus Macrobrachium Bate, 1868 from Kerala, India. Zootaxa, 3664 (4), 434–444.
https://doi.org/10.11646/zootaxa.3664.4.2
Pillai, P.M. & Unnikrishnan, V. (2013b) A replacement name for Macrobrachium aemulum madhusoodani, a junior homonym of Macrobrachium madhusoodani Unnikrishnan et al. 2011 (Decapoda: Palaemonidae). Zootaxa, 3741 (3), 400– 400.
https://doi.org/10.11646/zootaxa.3741.3.9
Pillai, P.M., Unnikrishnan, V. & Sureshkumar, U. (2014) Description, DNA barcode and phylogeny of a new species, Macrobrachium abrahami (Decapoda: Palaemonidae) from Kerala, India. Zootaxa, 3768 (5), 546– 556.
https://doi.org/10.11646/zootaxa.3768.5.2
Pillai, P.M., Unnikrishnan, V. & Prasannan, K. (2015) Macrobrachium indianum (Decapoda: Palaemonidae), a new species of hill stream prawn from Pambar River, Kerala, India. Zootaxa, 3936 (4), 559–566. https://doi.org/10.11646/zootaxa.3936.4.5
Radhakrishnan, E.V., Deshmukh, V.D., Maheswarudu, G., Josileen, J., Dineshbabu, A.P. Philipose, K.K., Sarada, P.T., Lakshmi Pillai, S., Saleela, K.N., Chakraborty, R., Dash, G., Sajeev, C.K., Thirumilu, P., Sridhara, B., Muniyappa, Y., Sawant, A.D., Vaidya, N.G. Dias Johny, R., Verma, J.B., Baby, P.K., Unnikrishnan, C., Ramachandran, N.P., Vairamani, A., Palanichamy, A., Radhakrishnan, M. & Raju, B. (2012) Prawn fauna (Crustacea: Decapoda) of India - An annotated checklist of the Penaeoid, Sergestoid, Stenopodid and Caridean prawns. Journal of the Marine Biological Association of India, 54 (1),50–72.
Rossi, N., Magalhães, M., Mesquita, E.R. & Mantelatto, F.L. (2020) Uncovering a hidden diversity: a new species of freshwater shrimp Macrobrachium (Decapoda: Caridea: Palaemonidae) from Neotropical region (Brazil) revealed by morphological review and mitochondrial genes analyses. Zootaxa, 4732 (1), 177–195.
https://doi.org/10.11646/zootaxa.4732.1.9
Sambrook, J., Fristisch, E.F. & Maniatis, T. (1989) Molecular cloning: A laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York, 1626 pp.
Saengphan, N., Panijpan, B., Senapin, S., Laosinchai, P., Ruenwongsa, P., Suksomnit, A. & Phiwsaiya, K. (2018) Morphology and molecular phylogeny of Macrobrachium suphanense sp. nov. (Decapoda: Palaemonidae) from Thailand. Zootaxa, 4482 (1), 151–163.
https://doi.org/10.11646/zootaxa.4482.1.7
Saengphan, N., Panijpan, B., Senapin, S., Laosinchai, P., Suksomnit, A. & Phiwsaiya, K. (2020) Morphology and molecular phylogeny of Macrobrachium saengphani sp. nov.(Decapoda: Palaemonidae) from Northern Thailand Zootaxa, 4868 (4), 531–542.
https://doi.org/10.11646/zootaxa.4868.4.4
Short, J.W. (2004) A revision of Australian river prawns, Macrobrachium (Crustacea: Decapoda: Palaemonidae). Hydrobiology, 525 (1–3), 1–100.
https://doi.org/10.1023/B:HYDR.0000038871.50730.95
Tamura, K., Stecher, G., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.
https://doi.org/10.1093/molbev/mst197
Xuan, N.V. (2012) Macrobrachium hungi, a new freshwater palaemonid prawn (Decapoda: Caridea: Palaemonidae) from the Tonle Sap Great Lake of Cambodia. Zootaxa, 3560 (1), 32–40.
https://doi.org/10.11646/zootaxa.3560.1.2
Zheng, X., Chen, W. & Guo, Z. (2019) The genus Macrobrachium (Crustacea, Caridea, Palaemonidae) with the description of a new species from the Zaomu Mountain Forest Park, Guangdong Province, China. ZooKeys, 866, 65–83.
https://doi.org/10.3897/zookeys.866.32708