Abstract
Two new fossil hard ticks (Arachnida: Ixodida: Ixodidae) from Dominican amber (Miocene, probably Burdigalian, ca. 16 Ma) are described. An almost fully engorged female and a partly engorged larva were documented by combining light microscopy with high-resolution µCT-scans at the Deutsches Elektronen-Synchrotron (DESY) and 3D modelling of the data. Images of the fossils created from these 3D models facilitated comparisons with living ticks and the new material can be assigned to the Recent genus Amblyomma C.L. Koch, 1844 as Amblyomma arawakan Chitimia-Dobler & Martins sp. nov. The new amber species appears to be most closely related to the extant New World tick Amblyomma auricularium Conil, 1878. Possible hosts for the fossils are discussed within the wider context of their historical biogeography.
References
- Amorim, M. & Serra-Freire, N.M. (2000) Morphological description of tick larval stage (Acari: Ixodidae). 7. Amblyomma auricularium (Conil, 1878). Entomología y Vectores, 3, 67–81.
- Barros-Battesti, D.M., Arzua M. & Bechara, G.H. (2006) Carrapatos de importância medico-veterinária da região neotropical: um guia ilustrado para identificação de espécies. Vox/ICTTD-3/Butantan, São Paulo, 223 pp.
- Barros-Battesti, D.M., Onofrio, V.C., Dantas-Torres, F. & Guglielmone. A.A. (2024) Família Ixodidae Koch, 1844. In: Barros-Battesti, D.M., Machado, R.Z. & André, M.R. (Eds), Ectoparasitofauna Brasileira de Importância Veterinária—Volume 3. Acarofauna de Importância Veterinária: Parasitiformes—Ixodida, Parte I. Colégio Brasileiro de Parasitologia Veterinária, Jaboticabal, 537 pp. https://doi.org/10.7476/9786583181664
- Beati, L. & Klompen H. (2019) Phylogeography of ticks (Acari: Ixodida). Annual Review of Entomology, 64, 379–397. https://doi.org/10.1146/annurev-ento-020117-043027
- Bermúdez, S., Apanaskevich, D. & Domínguez, L. (2018) Garrapatas Ixodidae de Panamá. SENACYT, Panama City, 129 pp.
- Camicas, J.L., Hervy, J.P., Adam, F. & Morel, P.C. (1998) Les tiques du monde (Acarida, Ixodida). Nomenclature, stades décrits, hôtes, répartition. ORSTOM, Paris, 233 pp.
- Chitimia-Dobler, L., De Araujo, B.C., Ruthensteiner, B., Pfeffer, T. & Dunlop, J.A. (2017) Amblyomma birmitum a new species of hard tick in Burmese amber. Parasitology, 144, 1441–1448. https://doi.org/10.1017/S0031182017000853
- Chitimia-Dobler, L., Mans, B.J., Handschuh, S. & Dunlop, J.A. (2022) A remarkable assemblage of ticks from mid-Cretaceous Burmese amber. Parasitology, 149, 820–830. https://doi.org/10.1017/S0031182022000269
- Chitimia-Dobler, L., Handschuh, S., Dunlop, J.A., Pienaar, R. & Mans, B.J. (2024) Nuttalliellidae in Burmese amber: implications for tick evolution. Parasitology, 151, 146–152. https://doi.org/10.1017/S0031182024000477
- de la Fuente, J., Estrada-Peña, A., Labruna, M.B. & Szabó, M.P (2024) Interaction between spiders and ticks—ancient arthropod predatory behavior? Parasitology Research, 123, 264. https://doi.org/10.1007/s00436-024-08282-2
- Domínguez, A.L. & Bermúdez, C.S. (2020) First records of abnormalities and gynandromorphism in hard ticks (Ixodida: Ixodidae) from Panama. Systematic and Applied Acarology, 25, 1199–1208. https://doi.org/10.11158/saa.25.7.4
- Dunlop, J.A., Apanaskevich, D.A., Lehmann, J., Hoffmann, R., Fusseis, F., Ehlke, M., Zachow, S. & Xiao, X.H. (2016) Microtomography of the Baltic amber tick Ixodes succineus reveals affinities with the modern Asian disease vector Ixodes ovatus. BMC Evolutionary Biology, 16, 203. https://doi.org/10.1186/s12862-016-0777-y
- Greving, I., Wilde, F., Ogurrek, M., Herzen, J., Hammel, J.U., Hipp, A., Friedrich, F., Lottermoser, L., Dose, T., Burmeseter, H., Müller, M. & Beckman, F. (2014) P05 imaging beamtime at PETRA III: first results. Proceedings of SPIE 9212, Developments in X-Ray Tomography, IX, 921200. https://doi.org/10.1117/12.2061768
- Guglielmone, A.A., Nava, S. & Robbins, R.G. (2021) Neotropical hard ticks (Acari: Ixodida: Ixodidae). Springer International Publishing, Berlin, 512 pp. https://doi.org/10.1007/978-3-030-72353-8
- Guglielmone, A.A., Nava, S. & Robbins, R.G. (2023) Geographic distribution of the hard ticks (Acari: Ixodida: Ixodidae) of the world by countries and territories. Zootaxa, 5251 (1), 1–274. https://doi.org/10.11646/zootaxa.5251.1.1
- Guglielmone, A.A., Petney, T.N. & Robbins, R.G. (2020) Ixodidae (Acari: Ixodoidea): descriptions and redescriptions of all known species from 1758 to December 31, 2019. Zootaxa, 4871 (1), 1–322. https://doi.org/10.11646/zootaxa.4871.1.1
- Guglielmone, A.A., Estrada-Peña, A., Luciani, C.A., Mangold, A.J. & Keirans, J.E. (2003) Hosts and distribution of Amblyomma auricularium (Conil, 1878) and Amblyomma pseudoconcolor Aragão, 1908 (Acari: Ixodidae). Experimental and Applied Acarology, 29, 131–139. https://doi.org/10.1023/A:1024251020035
- Guglielmone, A.A., Robbins, R.G., Apanaskevich, D.A., Petney, T.N., Estrada-Peña, A. & Horak, I.G. (2014) The hard ticks of the world (Acari: Ixodida: Ixodidae). Springer, Dordrecht, xiii + 738 pp. https://doi.org/10.1007/978-94-007-7497-1
- Guglielmone, A.A., Robbins, R.G., Apanaskevich, D.A., Petney, T.N., Estrada-Peña, A., Horak, I.G., Shao, R. & Barker, S.C. (2010) The Argasidae, Ixodidae and Nutalliellidae (Acari: Ixodida) of the world: a list of valid species names. Zootaxa, 2528 (1), 1–28. https://doi.org/10.11646/zootaxa.2528.1.1
- Hoogstraal, H. & Aeschlimann, A. (1982) Tick-host specificity. Bulletin de la Société Entomologique Suisse, 55, 5–32.
- Iturralde-Vinent, M.A. & MacPhee, R.D. (2019) Remarks on the age of Dominican amber. Palaeoentomology, 2 (3), 236–240. https://doi.org/10.11646/palaeoentomology.2.3.7
- Keirans, J.E., Lane, R.S. & Cauble, R. (2002) A series of larval Amblyomma species (Acari: Ixodidae) from amber deposits in the Dominican Republic. International Journal of Acarology, 28, 61–66. https://doi.org/10.1080/01647950208684282
- Keirans, J.E. (2009) Order Ixodida. In: Krantz, G.W. & Walter, D.E. (Eds), A Manual of Acarology. Third edition. Texas Tech University Press, Lubbock, pp. 111–123.
- Klompen, H. & Grimaldi, D. (2001) First Mesozoic record of a parasitiform mite: a larval argasid tick in Cretaceous amber (Acari: Ixodida: Argasidae). Annals of the Entomological Society of America, 94, 10–15. https://doi.org/10.1603/0013-8746(2001)094[0010:FMROAP]2.0.CO;2
- Kluyber, D., Martins, T.F., Desbiez, A.J.L., Amorim, M., Massocato, G. & Labruna, M.B., (2016) Ticks (Acari: Ixodidae) infesting armadillos (Cingulata: Dasypodidae) in the Pantanal wetland, Mato Grosso do Sul, Brazil. Systematic and Applied Acarology, 21, 1087–1091. https://doi.org/10.11158/saa.21.8.9
- Koch, C.L. (1844) Systematische Übersicht über die Ordnung der Zecken. Archiv für Naturgeschichte, 10, 217–239. https://doi.org/10.5962/bhl.part.29560
- Lambert, J. B., Frye, J.S. & Poinar, Jr. G.O. (1985) Amber from the Dominican Republic: analysis by nuclear magnetic resonance spectroscopy. Archaeometry, 27, 43–51. https://doi.org/10.1111/j.1475-4754.1985.tb00345.x
- Lane, R.S. & Poinar, Jr. G.O. (1986) First fossil tick (Acari: Ixodidae) in New World amber. International Journal of Acarology, 12, 75–78. https://doi.org/10.1080/01647958608683444
- Lamarck, J.B. (1801) Système des animaux sans vertèbres, ou tableau général des classes, des ordres et des genres de ces animaux. Published by the author and Deterville, Paris, viii + 432 pp. https://doi.org/10.5962/bhl.title.14255
- Lord, C.C. & Day, J.F. (2000) First record of Amblyomma auricularium (Acari: Ixodidae) in the United States. Journal of Medical Entomology, 37, 977–978. https://doi.org/10.1603/0022-2585-37.6.977
- Lösel, P.D., Monchanin, C., Lebrun, R., Jayme, A., Relle, J.J., Devaud, J.M., Heuveline, V. & Lihoreau, M. (2023) Natural variability in bee brain size and symmetry revealed by micro-CT imaging and deep learning. PLOS Computational Biology, 19 (10), e1011529. https://doi.org/10.1371/journal.pcbi.1011529
- Luz, H.R., Labruna, M.B., Pacheco, R.C., Gianizella, S.L., Nunes, P.H., Szabó, M.P.J., Gerardi, M., Teixeira, R.H.F., da Silva, S.C., Kmetiuk, L.B., Pesenato, I.P., Marcili, A., Faccini, J.L.H. & Martins, T.F. (2023) Morphological anomalies in hard ticks (Acari: Ixodidae) from Brazil. Ticks and Tick Borne Diseases, 14 (6), 102219. https://doi.org/10.1016/j.ttbdis.2023.102219
- Lytaev, P., Hipp, A., Lottermoser, L., Herzen, J., Greving, I., Khokhriakov, I., Meyer-Loges, S., Plewka, J., Burmester, J., Caselle, M., Vogelgesang, M., Chilingaryan, S., Kopmann, A., Balzer, M., Schreyer, A. & Beckmann, F. (2014) Characterization of the CCD and CMOS cameras for grating-based phase-contrast tomography. Proceedings of SPIE 9212, Developments in X-Ray Tomography, IX, 921218. https://doi.org/10.1117/12.2061389
- Martins, T.F., Onofrio, V.C., Labruna, M.B., Guglielmone, A.A. & Barros-Battesti, D.M. (2024) Família Ixodidae da Região Neotropical: Características Gerais, Distribuição Geográfica, Hospedeiros e Chaves de Identificação das Espécies que Ocorrem no Brasil. In: Barros-Battesti, D.M., Machado, R.Z. & André, M.R. (Eds), Ectoparasitofauna Brasileira de Importância Veterinária—Volume 3. Acarofauna de Importância Veterinária: Parasitiformes—Ixodida, Parte I. 1 ed. Colégio Brasileiro de Parasitologia Veterinária, Jaboticabal, 537 pp.
- Martins, T.F., Yogui, D.R., Alves, M.H., Kluyber, D., Massocato, G., Labruna, M. B. & Desbiez, A.L. (2023) Surveillance of ticks (Acari: Ixodidae) on monitored free-ranging giant anteaters and on road-killed anteaters and armadillos in the State of Mato Grosso do Sul, Midwestern Brazil. Systematic and Applied Acarology, 28, 641–646. https://doi.org/10.11158/saa.28.4.1
- Mertins, J.W., Vigil, S.L. & Corn, J.L. (2017) Amblyomma auricularium (Ixodida: Ixodidae) in Florida: new hosts and distributional records. Journal of Medical Entomology, 54, 132–141. https://doi.org/10.1093/jme/tjw159
- Moosmann, J., Ershov, A., Weinhardt, V., Baumbach, T., Prasad, M.S., LaBonne, C., Xiao, X., Kashef, J. & Hofmann, R. (2014) Time-lapse X-ray phase-contrast microtomography for in vivo imaging and analysis of morphogenesis. Nature Protocols, 9, 294–304. https://doi.org/10.1038/nprot.2014.033
- Murray, A. (1877) Economic entomology. Aptera. Chapman and Hall, London, xxiii + 433 pp. https://doi.org/10.5962/bhl.title.51877
- Nava, S., Venzal, J.M., González-Acuña, D., Martins, T.F. & Guglielmone, A.A. (2017) Ticks of the Southern Cone of America: diagnosis, distribution and hosts with taxonomy, ecology and sanitary importance. Academic Press, London, 348 pp.
- Palenstijn, W.J., Batenburg, K.J. & Sijbers, J. (2011) Performance improvements for iterative electron tomography reconstruction using graphics processing units (GPUs). Journal of Structural Biology, 176, 250–253. https://doi.org/10.1016/j.jsb.2011.07.017
- Penney, D. (2010) Dominican amber. In: Penney, D. (Ed.), Biodiversity of fossils in amber from the major world deposits. Siri Scientific Press, Manchester, pp. 22–41.
- Perez-Gelabert, D.E. (2020) Checklist, bibliography and quantitative data of the arthropods of Hispaniola. Zootaxa, 4749 (1), 1–668. https://doi.org/10.11646/zootaxa.4749.1.1
- Poinar, Jr. G.O. (1995) First fossil soft tick, Ornithodoros antiquus n. sp. (Acari: Argasidae) in Dominican amber with evidence of their mammalian host. Experimentia, 51, 584–587. https://doi.org/10.1007/BF01928900
- Poinar, Jr. G.O. (2017) Fossilized mammalian erythrocytes associated with a tick reveal ancient piroplasms. Journal of Medical Entomology, 54, 895–900. https://doi.org/10.1093/jme/tjw247
- Poinar, Jr. G.O. & Ricci, C. (1992) Bdelloid rotifers in Dominican amber: Evidence for parthenogenetic continuity. Experientia, 48, 408–410. https://doi.org/10.1007/BF01923444
- Reuter, E. (1909) Zur Morphology und Ontogenie der Acariden: Mit besonderer Berücksichtigung von Pediculopsis Graminum (E. Reut). Acta Societatis Scientiarum Fennicae, 36 (4), i + iv + 1–288 + 6 pls. https://doi.org/10.5962/bhl.title.4705
- Robinson, L.E. (1926) Ticks. A monograph of the Ixodoidea, Part IV. The genus Amblyomma. Cambridge University Press, Cambridge, 302 pp. https://doi.org/10.5962/bhl.title.24074
- Sanchez, J.P., Nava, S., Lareschi, M., Ortiz, P.E. & Guglielmone, A.A. (2010) Finding of an ixodid tick inside a late Holocene owl pellet from northwestern Argentina. Journal of Parasitology, 96, 820–822. https://doi.org/10.1645/GE-2392.1
- Santos Dias, J.A.T. (1993) Nova contribucao para o estudo da sistemática do género Amblyomma Koch, 1844 (Acarina-Ixodoidea). Garcia de Orta, Série de Botânica, 9, 11–19
- Stach, P., Natkaniec-Nowak, L., Dumańska-Słowik, M., Kosakowski, P., Naglik, B., Drzewicz, P., Misiak, J., Pršek, J., George, C. & Ramírez Gómez, R.E. (2021) The study of Dominican amber-bearing sediments from Siete Cañadas and La Cumbre with a discussion on their origin. Scientific Reports, 11, 18556. https://doi.org/10.1038/s41598-021-96520-3
- Uribe, J.E., Kelava, S., Nava, S., Cotes-Perdomo, A.P., Castro, L.R., Rivera-Paéz, F.A., Perea, S., Mans, B.J., Gofton, A., Teo, E.J.M., Zardoya, R. & Barker, S.C. (2024) New insights into the molecular phylogeny, biogeographical history, and diversification of Amblyomma ticks (Acari: Ixodidae) based on mitogenomes and nuclear sequences. Parasites & Vectors, 17, 139. https://doi.org/10.1186/s13071-024-06131-w
- Van Aarle, W., Palenstijn, W.J., Cant, J., Janssens, E., Bleichrodt, F., Dabravolski, A., De Beenhouwer, J., Batenburg, K.J. & Sijbers, J. (2016) Fast and flexible X-ray tomography using the ASTRA toolbox. Optics Express, 24, 25129–25147. https://doi.org/10.1364/OE.24.025129
- Van Aarle, W., Palenstijn, W.J., De Beenhouwer, J., Altantzis, T., Bals, S., Batenburg, K.J. & Sijbers, J. (2015) The ASTRA Toolbox: a platform for advanced algorithm development in electron tomography. Ultramicroscopy, 157 (7), 35–47. https://doi.org/10.1016/j.ultramic.2015.05.002
- Vinent, M.A.I. & MacPhee, R.D. (2023) New evidence for late Eocene-early Oligocene uplift of Aves Ridge and paleogeography of GAARlandia. Geologica Acta, 21 (5), 1–10. https://doi.org/10.1344/GeologicaActa2023.21.5
- Viñola-Lopez, L.W., Suárez, E.E.C., Vélez-Juarbe, J., Milan, J.N.A. & Bloch, J.I. (2022) The oldest known record of a ground sloth (Mammalia, Xenarthra, Folivora) from Hispaniola: evolutionary and paleobiogeographical implications. Journal of Paleontology, 96, 684–691. https://doi.org/10.1017/jpa.2021.109
- Voltzit, O.V. (2007) A review of Neotropical Amblyomma species (Acari: Ixodidae). Acarina, 15, 3–134.
- Weidner, H. (1964) Eine Zecke, Ixodes succineus sp. n. im Batischen Bernstein. Veöffentlichungen aus dem Überseemuseum Bremen, 3, 143–151.
- Wilde, F., Ogurreck, M., Greving, I., Hammel, J.U., Beckmann, F., Hipp, A., Lottermoser, L., Khokhriakov, I., Lytaev, P., Dose, T., Burmester, H., Müller, M. & Schreyer, A. (2016) Micro-CT at the imaging beamline P05 at PETRA III. In: AIP Conference Proceedings, 1741, 030035. https://doi.org/10.1063/1.4952858
- Woods, C.A. (1981) Last endemic mammals in Hispaniola. Oryx, 16 (2), 146–152. https://doi.org/10.1017/S0030605300017105
