Abstract
The taxonomic position of Hetrodes pupus (Tettigoniidae, Hetrodinae) was clarified by a cytogenetic study of mitotic and meiotic chromosomes, including the localization of heterochromatin by C-banding. The karyotype of this species consists of 26 acrocentric autosomes and an acrocentric X chromosome, the largest element of the karyotype (2n male=27, FN=27; sex determination system is X0 male/XX female). A cytogenetic comparative analysis of the Hetrodinae and other katydids revealed (1) the similarity of the karyotypic features of Hetrodinae and Microtettigoninae, indicating monophyly; (2) that the morphology of the Hetrodinae karyotype is the result of parallel chromosome evolution via a decrease in the number of chromosome arms (FN).References
Asana, J.J., Makino, S. & Niyama, H. (1938) A chromosomal survey of some Indian insects. I. Morphology of the chromosomes in eight species of the Locustidae. Journal of the Faculty of Science, Hokkaido Imperial University, Series 6, Zoology, 6, 221–234.
Aswathanarayana, N.V. & Aswath, S.K. (1994) Karyotypes of two Indian grasshoppers of Mecopodinae (Orthoptera-Tettigoniidae). Cytologia, 59, 285–287.
Bailey, W.J. & Rentz, D.C.F. (1990) The Tettigoniidae: their diversity and importance in biological research. In: Bailey, W.J. & Rentz, D.C.F. (Eds), The Tettigoniidae – Biology, Systematics, and Evolution. Springer-Verlag. Hong Kong, 395 pp.
Bianchi, A.P. & Montalenti, S.G. (1966) Note sulla cariologia di alcuni Efippigeridi (Insecta -Orthoptera). Accademia Nazionale dei Lincei, Rendiconti della Classe di Scienze fisiche, matematiche e naturali. Serie 8, 41, 553–557.
Bugrov, A.G., Warchalowska-Sliwa, E., Ito, G., Tchernykh, A. & Maryati, M. (2004) Karyotype and C-banding patterns of the katydid Mecopoda elongata (L.) (Orthoptera, Tettigoniidae, Mecopodina) from Amami Is. (Japan) and Borneo (Malaysia). Caryologia, 57, 25–29.
Bullini, L. & Bianchi-Bullini, A.P. (1969) Nuoi dati sul corredo cromosomico degli Efippigeridi italiani (Orthoptera – Tettigonioidea). Accademia Nazionale dei Lincei, Rendiconti della Classe di Scienze fisiche, matematiche e naturali. Serie 8, 46, 213–217.
Darlington, C.D. & La Cour, L.F. (1976) The Handling of Chromosomes. London: Alle & Unwin. 6th ed. 201pp.
Eades, D.C. & D. Otte (2007) Orthoptera Species File Online. Version 2.0/3.4. [retrieval date]. <http://Orthoptera.SpeciesFile.org
Favrelle, M. (1936) Contribuion a l’etude de la garniture chromosomique des orthopteres (Gryllidae et Tettigoniidae). Annals D’Musée Royale D’ Histoire Naturelle de Belgique, Bruxelles Ser.2, 3, 53–60.
Fernandez-Piqueras, J., Rodriquez A., Sentis C. & Rojo E. (1984). C-heterochromatin variation in the monospecific genus Baetica (Orthoptera: Tettigoniidae). Caryologia, 37, 69–76.
Gorochov, A.V. (1988) The classification and phylogeny of grasshoppers (Gryllidae-Orthoptera, Tettigoniidae). In: Ponomarenko, A. (Ed.), The Cretaceous Biocoenotic Crisis and the Evolution of Insects. Nauka, Moscow, 145–190 pp. (In Russian).
Gorochov, A.V. (1995) System and evolution of the suborder Ensifera (Orthoptera). Russian Academy of Sciences, Proceedings of the Zoological Institute, St. Petersburg, 260, Part 1, pp. 224 (In Russian).
Gwynne, D.T. (1995) Phylogeny of the Ensifera (Orthoptera): a hypothesis supporting multiple origins of acoustical signaling, complex spermatophores and maternal care in crickets, katydids, and weta. Journal of Orthoptera Research, 203–218.
Irish, J. (1992) The Hetrodinae (Orthoptera: Ensifera: Bradyporidae) of southern Africa: systematics and phylogeny. Navorsinge van die Nasionale Museum Bloemfontein, 8, 393–434.
Ju-Chi, Li (1930–31) Spermatogenesis and chromosomes of Callimenus onos Pallas (Tettigoniidae, Orthoptera). Peking Natural History Bulletin, 5, 1–17.
Kevan, D.K.M. (1977) The higher classification of Orthopteroids insects: A general view. Memoirs of the Lyman Entomological Museum and Research Laboratory, 4, 1–31.
Kevan, D.K.M. (1982) Orthoptera. In: Parker, S.P. (Ed.), Synopsis and Classification of Living Organisms. McGraw-Hill, New York, pp. 352–383.
Matthey, R. (1948) Données nouvelles sur les chromosomes des Tettigonides et la parthénogénèse de Saga pedo Pallas. Revue Suisse de Zoology , 55, 45–56.
Matthey, R. (1950) Les chromosomes de Saga cappadocica Werner (Orthoptera: Tettigoniidae). Jahresbericht der Schweizerischen Geseltschaft für Vererbungsforschung, Societe Suisse de Genetique (S.S.G.), 9, 44–46.
Mbata, K.J. (2005) A contribution to the cytogenetics of the armoured ground cricket, Acanthoplus speiseri (Orthoptera: Tettigoniidae), a pest of grain crops in Zambia. International Journal of Tropical Insect Science, 25, 142–145.
Nickle, D.A., & Naskrecki P.A. (1997) Recent developments in the systematics of Tettigoniidae and Gryllidae. In: Gangwere, S.K., Muralirangan, M.C. & Muralirangan, M. (Eds.), The Bionomics of Grasshoppers, Katydids and Their Kin, CAB International, pp. 41–58.
Rentz, D.C.F (1979) Comments on the classiffication of the orthopteran family Tettigoniidae, with a key to subfamilies and description of two new subfamilies. Australian Journal of Zoology, 27, 991–1013.
Sentis, C., Santos, J. & Fernandez-Piqueras J. (1988) Primary and secondary nucleolar organizer regions during spermatogenesis in the genus Pycnogaster. Heredity, 60, 197–204.
Sumner, S.G. (1972) A simple technique for demonstrating centromere heterochromatin. Experimental Cell Research, 75, 304–306.
Ueshima, N. (1993) Karyotypes and meiosis of Phasmodinae, Zaprochilinae and Austrosaginae. In: Rentz, D.C.F. (Ed.), A Monograph of the Tettigoniidae of Australia, Vol. 2. The Phasmodinae, Zaprochilinae and Austrosaginae. SSIRO Publishing, East Melbourne, pp. 345–358.
Ueshima, N. & Rentz, D.C.F. (1990) Karyotypes and meiosis of the Australian Tettigoniinae. In: Bailey, W.J. & Rentz, D.C.F. (Eds.) The Tettigoniidae. Biology, Systematics and Evolution. Springer Verlag, Hong Kong, pp. 303–352.
Warchałowska-Śliwa, E. (1998) Karyotype characteristics of katydid orthopterans (Ensifera, Tettigoniidae), and remarks on their evolution at different taxonomic levels. Folia biologica (Kraków), 46, 143–176.
Warchałowska-Śliwa, E., & Bugrov, A.G. (1996) Karyotypes and C-banding patterns of Bradyporinae (Orthoptera, Tettigoniidae). Folia biologica (Kraków), 44, 95–98.
Warchałowska-Śliwa, E., & Bugrov, A.G. (1997). C-heterochromatin variation in Deracantha onos (Pall.) (Deracanthini, Bradyporinae, Tettigoniidae, Orthoptera). Cytologia, 62, 7–12.
Warchałowska-Śliwa, E.& Bugrov, A.G. (1998) Karyotypes and C-banding patterns of some Phaneropterinae katydids (Orthoptera, Tettigonioidea) with special attention to a post-reductional division of the neo-X and the neo-Y sex chromosomes in Isophya hemiptera. Folia biologica (Kraków), 46, 47–54.
Warchałowska-Śliwa, E., Heller, K-G. & Maryańska-Nadachowska, A. 2005. Cytogenetic variability of European Tettigoniinae (Orthoptera, Tettigoniidae): karyotypes, C-and Ag-NOR-banding. Folia biologica (Kraków) 53, 161–171.
Warchałowska-Śliwa, E., Maryańska-Nadachowska, A.& Heller, K-G. (2007) Cytogenetic variability of the genus Saga Charp. (Orthoptera, Tettigoniidae, Saginae): heterochromatin distribution. Caryologia, 60, 29–36.
Warchałowska-Śliwa, E., Grzywacz, B., Maryańska-Nadachowska, A., Karamysheva, T.V., Rubtsov, N.B. & Chobanov D.P. (2009) Chromosomal differentiation among bisexual European species of Saga Charp. (Orthoptera, Tettigoniidae, Saginae) detected by both classical and molecular methods. European Journal of Entomology, 106: 1–9.
White, M.J.D. (1973) Animal Cytology and Evolution. Cambridge University Press, London, 961 pp.
Zeuner, F.E. (1936) The subfamilies of Tettigoniidae (Orthoptera). Transactions of the Royal Society of London, Series B: Biological Sciences (London), 5, 103–109.