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Type: Articles
Published: 2010-08-30
Page range: 30–44
Abstract views: 89
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Mitochondrial DNA and morphometrical identification of a new species of Hylomyscus (Rodentia: Muridae) from West Africa

Muséum National d’Histoire Naturelle, Département de Systématique et Evolution, UMR 7205, Laboratoire Mammifères et Oiseaux, 57 rue Cuvier, CP 51, 75005 Paris, France
Natural History Museum, Obafemi Awolowo University, Ile-Ife, Osun, Nigeria
Royal Museum for Central Africa, Zoology Department, Vertebrate section, Leuvensesteenweg 13, Tervuren, Belgique
UMR CNRS 6553 Ecobio, Université de Rennes 1, Station Biologique, 35380 Paimpont, France
Mammalia 16S rDNA Benin Cytochrome b Nigeria sibling species taxonomy traditional morphometrics tropical African forests woodmice

Abstract

In this paper we describe a new species of Hylomyscus based on molecular (Cyt b and 16S rDNA gene sequencing) and morphometrical analyses. This new species occurs in Benin and Nigeria, and probably also in Togo. It differs by 8.29 to 10.40 % of sequence divergence (K2P distance for the Cyt b gene) from all other species of the H. alleni species complex, and can be distinguished from these species through morphometrical multivariate analyses. It differs significantly from its closest relative, H. simus, by four external and nine cranio-dental measurements. The role of rivers and Pleistocene forest refugia in the diversification of the H. alleni species complex is discussed.

References

  1. Avise, J.C. (2000) Phylogeography: the history and formation of species. Harvard University press, Cambridge, 447 pp.

    Avise, J.C., Shapiro, J.F., Daniel, S.W., Aquadro, C.F. & Lansman, R.A. (1983) Mitochondrial DNA differentiation during the speciation process in Peromyscus. Molecular Biology and Evolution, 1, 38–56.

    Barome, P.O., Monnerot, M. & Gautun, J.C. (1998) Intrageneric phylogeny of Acomys (rodentia, muridae) using mitochondrial gene cytochrome b. Molecular Phylogenetics and Evolution, 9, 560–6.

    Bradley, R.D. & Baker, A. (2001) A test of the genetic species concept: cytochrome-b sequences and mammals. Journal of Mammalogy, 82, 960–973.

    Burk, A., Douzery, E.J.p. & Springer, M.S. (2002) The secondary structure of mammalian mitochondrial 16S rRNA molecules: refinements based on a comparative phylogenetic approach. Journal of Mammalian Evolution, 9, 225–252.

    Carleton, M.D., Kerbis Peterhans, J.C. & Stanley, W.T. (2006) Review of the Hylomyscus denniae group (Rodentia: Muridae) in Eastern Africa, with comments on the generic allocation of Epimys endorobae Heller. Proceedings of the Biological Society of Washington, 119, 293.

    Carleton, M.D. & Stanley, W.T. (2005) Review of the Hylomyscus denniae complex (Rodentia: Muridae) in Tanzania, with a description of a new species. Proceedings of the Biological Society of Washington, 118, 619–646.

    Colyn, M. & Deleporte, P. (2002) Biogeographic analysis of Central African forest guenons. In: Colyn, M. & Deleporte, P. (Eds.)

    Colyn, M., Gautier-Hion, A. & Verheyen, W. (1991) A re-appraisal of palaeoenvironmental history in Central Africa: evidence for a major fluvial refuge in the Zaire Basin. Journal of Biogeography, 18, 403–407.

    Costa, L.P. (2003) The historical bridge between the Amazon and the Atlantic Forest of Brazil: a study of molecular phylogeography with small mammals. Journal of Biogeography, 30, 71–86.

    de Queiroz, K. (1998) The general lineage concept of species, species criteria and the process of speciation. A conceptual unification and terminological recommendations. In: Howard, D.J. & Berlocher, S.H. (Eds.) Endless forms: species and speciation. Oxford University Press, Oxford, pp. 57–75.

    Deleporte, P. & Colyn, M. (1999) Biogéographie et dynamique de la biodiversité: application de la PAE aux forêts planitiaires d’Afrique centrale. Biosystema, 17, 37–43.

    Ducroz, J.F., Volobouev, V. & Granjon, L. (2001) An assessment of the systematics of Arvicanthine rodents using mitochondrial DNA sequences: evolutionary and biogeographical implications. Journal of Mammalian Evolution, 8, 173–206.

    Dupont, L.M., Jahns, S., Marret, F. & Ning, S. (2000) Vegetation change in equatorial West Africa: time-slices for the last 150 ka. Palaeogeography, Palaeoclimatology, Palaeoecology, 155, 95–122.

    Dupont, L.M. & Weinelt, M. (1996) Vegetation history of the savanna corridor between the Guinean and the Congolian rain forest during the last 150,000 years. Vegetation History and Archaeobotany, 5, 273–292.

    Ern, H. (1988) Flora and vegetation of the Dahomey-Gap - A contribution to the plant geography of West tropical Africa. Monographs in Systematic Botany from the Missouri Botanical Garden, 25, 517–520.

    Ferguson, J.W. (2002) On the use of genetic divergence for identifying species. Biological Journal of the Linnean Society, 75, 509–516.

    Grubb, P. (1990) Primate geography in the Afro-tropical forest biome. In: Peters, G. & Hutterer, R. (Eds.) Vertebrates in the tropics. Museum Alexander Koenig, Bonn, pp. 187–214.

    Guindon, S. & Gascuel, O. (2003) A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Systematic Biology, 52, 696–704.

    Guindon, S., Lethiec, F., Duroux, P. & Gascuel, O. (2005) PHYML Online- a web server for fast maximum likelihood-based phylogenetic inference. Nucleic Acids Research, 33, W557–9.

    Hall, J.B. & Swaine, M.D. (1981) Distribution and Ecology of vascular plants in a tropical rain forest, Forest Vegetation in Ghana. Dr W. Junk Publishers, The Hague-Boston-London, 392 pp.

    Happold, D.C.D. (1996) Mammals of the Guinea-Congo rainforest. In: Alexander, I.J., Swaine, M.D. & Watling, R. (Eds.) Essays on the ecology of the Guinea-Congo rain forest, 243–284.

    Harris, D., Rogers, D.S. & Sullivan, J. (2000) Phylogeography of Peromyscus furvus (Rodentia; muridae) based on cytochrome b sequence data. Molecular ecology, 9, 2129–35.

    Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics, 17, 754–5.

    Johns, G.C. & Avise, J.C. (1998) A comparative summary of genetic distances in the vertebrates from the mitochondrial cytochrome b gene. Molecular Biology and Evolution, 15, 1481–90.

    Klecka, W.R. (1980) Discriminant analysis. Sage Publications, London,pp.

    Kocher, T.D., Thomas, W.K., Meyer, A., Edwards, S.V., Paabo, S., Villablanca, F.X. & Wilson, A.C. (1989) Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proceedings of the National Academy of Sciences of the United States of America, 86, 6196–200.

    Lecompte, E., Granjon, L., Peterhans, J.K. & Denys, C. (2002) Cytochrome b-based phylogeny of the Praomys group (Rodentia, Murinae): a new African radiation? Comptes Rendus Biologies, 325, 827–40.

    Maddison, W.P. (1997) Gene trees in species trees. Systematic Biology 46, 523–536.

    Maley, J. (1991) The African rain forest vegetation and palaeoenvironments during late quaternary. Climatic Change, 19, 79–98.

    Maley, J. (1996) The African rain forest - main characteristics of changes in vegetation and climate from the Upper Cretaceous to the Quaternary. Proceedings of the Royal Society of Edinburgh, 104B, 31–73.

    Maley, J. (2001) The impact of arid phases on the African rain forest through geological history. In: Weber, W., White, L., Vedder, A. & Naughton-Treves, L. (Eds.) African rain forest ecology and conservation. Yale University Press68–87.

    Mayaux, P., Bartholome, E., Fritz, S. & Belward, A. (2004) A new land-cover map of Africa for the year 2000. Journal of Biogeography, 31, 861.

    Missoup, A.D., Nicolas, V., Wendelen, W., Bilong Bilong, C.F., Cruaud, C. & Denys, C. (2009) First record of Hylomyscus walterverheyeni on the north-western side of the Sanaga River (western Cameroon). Zootaxa, 2004, 46–60.

    Moore, W.S. (1995) Inferring phylogenies from mtDNA variation: mitochondrial-gene trees versus nuclear-gene trees. Evolution, 49, 718–726.

    Mouline, K., Granjon, L., Galan, M., Tatard, C., Abdoullaye, D., Ag Atteyine, S., Duplantier, J.M. & Cosson, J.F. (2008) Phylogeography of a Sahelian rodent species Mastomys huberti: a Plio-Pleistocene story of emergence and colonization of humid habitats. Molecular Ecology, 17, 1036–53.

    Musser, G.G. & Carleton, M.D. (2005) Superfamily Muroidea. In: Wilson, D.E. & Reeder, D.M. (Eds.) Mammal species of the world: a taxonomic and geographic reference. The Johns Hopkins University Press, Baltimore, pp. 894–1531.

    Nicolas, V. & Colyn, M. (2003) Seasonal variations in population and community structure of small rodents in a tropical forest of Gabon. Canadian Journal of Zoology, 81, 1034–1046.

    Nicolas, V., Quérouil, S., Verheyen, E., Verheyen, W., Mboumba, J.F., Dillen, M. & Colyn, M. (2006) Mitochondrial phylogeny of African wood mice, genus Hylomyscus (Rodentia, Muridae): implications for their taxonomy and biogeography. Molecular Phylogenetics and Evolution, 38, 779–93.

    Nicolas, V., Verheyen, E., Verheyen, W., Hulselmans, J., Dillen, M., Akpatou, B., Dudu, A., Wendelen, W. & Colyn, M. (2005) Systematics of African lowland rainforest Praomys (Rodentia, Muridae) based on molecular and craniometrical data. Zoological Journal of the Linnean Society, 145, 539–553.

    Nicolas, V., Wendelen, W., Barrière, P., Dudu, A. & Colyn, M. (2008) Morphometrical variation in Hylomyscus alleni and Hylomyscus stella (Rodentia, Muridae), and description of a new species. Journal of Mammalogy, 89, 222–231.

    Palumbi, S.R., Martin, A., Romano, S., McMillan, W.O., Stice, L. & Grabowski, G. (1991) The Simple Fool’s Guide to PCR. University of Hawaii Press, Honolulu,pp.

    Pamilo, P. & Nei, M. (1988) Relationships between gene trees and species trees. Molecular Biology and Evolution 5, 568–583.

    Peppers, L.L. & Bradley, R.D. (2000) Cryptic species in Sigmodon hispidus: evidence from DNA sequences. Journal of Mammalogy, 81, 332–343.

    Posada, D. & Crandall, K.a. (1998) MODELTEST: testing the model of DNA substitution. Bioinformatics, 14, 817–818.

    Quérouil, S., Hutterer, R., Barrière, P., Colyn, M., Peterhans, J.C.K. & Verheyen, E. (2001) Phylogeny and evolution of African shrews (Mammalia: Soricidae) inferred from 16s rRNA sequences. Molecular Phylogenetics and Evolution, 20, 185–195.

    Robbins, L.W., Choate, J.R. & Robbins, R.L. (1980) Nongeographic and interspecific variation in four species of Hylomyscus (Rodentia: Muridae) in Southern Cameroon. Annals of Carnegie Museum, 49, 31–48.

    Robert, C. & Chamley, H. (1987) Cenozoic evolution of continental humidity and paleoenvironment, deduced from kaolinite content of oceanic sediments. Palaeogeography, Palaeoclimatology and Palaeoecology, 60, 171–187.

    Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–4.

    Ronquist, F., Huelsenbeck, J.P. & Van der Mark, P. (2005) MrBayes 3.1 Manual. In: http://mrbayes.csit.fsu.edu/

    Rosevear, D. (1969) The Rodents of West Africa. Trustees of the British Museum (Natural History), London, 418 pp.

    Rosevear, D.R. (1966) The subgenus Hylomyscus Thomas in West Africa. 30, 13–24. Salzmann, U. & Hoelzmann, P. (2005) The Dahomey Gap: an abrupt climatically induced rain forest fragmentation in West Africa during the late Holocene. The Holocene, 15, 190–199.

    Slatkin, M. & Maddison, W.P. (1989) A cladistic measure of gene flow inferred from the phylogenies of alleles. Genetics, 123, 603–13.

    Statsoft, I. (2001) STATISTICA (data analysis software system), version 6. www.statsoft.com

    Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position- speciWc gap penalties, and weight matrix choice. Nucleic Acids Research, 22, 4673–4680.

    Winnepenninckx, B., Backeljau, T. & De Wachter, R. (1993) Extraction of high molecular weight DNA from molluscs. Trends in Genetics, 9, 407.