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Issue: Vol. 4085 No. 1: 1 Mar. 2016
Type: Article
Published: 2016-03-01
DOI: 10.11646/zootaxa.4085.1.3
Page range: 63–102
Abstract views: 102
PDF downloaded: 2

Five new, microendemic Asian Leaf-litter Frogs (Leptolalax) from the southern Annamite mountains, Vietnam

Australian Museum Research Institute, Australian Museum, 6 College St, Sydney NSW 2010, Australia.
Faculty of Biology - Biotechnology, Vietnam National University Ho Chi Minh city-University of Science, 227 Nguyen Van Cu, District 5, Ho Chi Minh City, Vietnam
Faculty of Biology - Biotechnology, Vietnam National University Ho Chi Minh city-University of Science, 227 Nguyen Van Cu, District 5, Ho Chi Minh City, Vietnam
Faculty of Natural Sciences Hong Duc University, No 565 Quang Trung Street- Dong Ve Ward - Thanh Hoa city, Vietnam.
Museu Paraense Emíllio Goeldi, CZO. Avenida Perimetral, 1.901, Terra Firme, CEP 66077-530, Belelém, Pará, Brasil
Faculty of Natural Sciences Hong Duc University, No 565 Quang Trung Street- Dong Ve Ward - Thanh Hoa city, Vietnam.
Faculty of Biology - Biotechnology, Vietnam National University Ho Chi Minh city-University of Science, 227 Nguyen Van Cu, District 5, Ho Chi Minh City, Vietnam
Vietnam National Museum of Nature, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet, Hanoi, Vietnam.
AG Zoologischer Garten Köln, Riehler Straße 173, D-50735 Köln, Germany
Amphibia Bioacoustics Leptolalax ardens sp. nov. Leptolalax kalonensis sp. nov. Leptolalax pallidus sp. nov. Leptolalax maculosus sp. nov. and Leptolalax tadungensis sp. nov. microendemism Southeast Asia

Abstract

The Leptolalax applebyi group of Asian leaf-litter frogs currently comprises four species of particularly small-bodied (<40 mm SVL) species distributed in the Central Highlands of Vietnam and northeastern Cambodia. In addition to their small size, the group is characterized by their morphological and genetic similarities, as well as their breeding habitat at headwaters of small mountain streams and seeps. A recent study suggested that at least two-thirds of the diversity of the group remained hidden within morphologically cryptic lineages. We expand upon the molecular, morphometric, and acoustic data and formally delineate and describe five of these lineages as distinct species: Leptolalax ardens sp. nov., Leptolalax kalonensis sp. nov., Leptolalax pallidus sp. nov., Leptolalax maculosus sp. nov., and Leptolalax tadungensis sp. nov. Due to habitat loss, the current ranges of these species are likely to be a fraction of their historical extent and under continued threat from deforestation.

 

References

  1. Boulenger, G.A. (1900) Descriptions of new batrachians and reptiles from the Larut Hills, Perak. Annals and Magazine of Natural History, Series 7, 6, 186–194.
    http://dx.doi.org/10.1080/00222930008678356

    Boulenger, G.A. (1893) Concluding report on the reptiles and batrachians obtained in Burma by Signor L. Fea, dealing with the collection made in Pegu and the Karin Hills in 1887-88. Annali del Museuo Civico di Genoa, 13, 304–347.

    Boulenger, G.A. (1908) A revision of the Oriental pelobatid batrachians (genus Megalophrys). Proceedings of the Zoological Society of London, 1908, 407–430.
    http://dx.doi.org/10.1111/j.1096-3642.1908.tb01852.x

    Cocroft, R.B. & Ryan, M.J. (1995) Patterns of advertisement call evolution in toads and chorus frogs. Animal Behaviour, 49, 283–303.
    http://dx.doi.org/10.1006/anbe.1995.0043

    Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: more models, new heuristics and parallel computing. Nature Methods, 9 (8), 772–772.

    Dehling, J.M. (2012) Eine neue Art der Gattung Leptolalax (Anura: Megophryidae) vom Gunung Benom, Westmalaysia/A new species of the genus Leptolalax (Anura: Megophryidae) from Gunung Benom, Peninsular Malaysia. Sauria, 34, 9–21.

    Dubois, A. (1983) Note preliminaire sur le genre Leptolalax Dubois, 1980 (Amphibiens, Anoures), avec diagnose d’une espece novelle du Vietnam. Alytes, 2, 147–153.

    Duellman, W.E. (1970) The Hylid Frogs of Middle America. Monograph of the Museum of Natural History, University of Kansas, 1, 1–427.

    Fei, L., Hu, S., Ye, C. & Huang, Y. (2009) Fauna Sinica. Amphibia. Vol. 2. Anura. Science Press, Beijing, 957 pp.

    Fei, L., Ye, C. & Huang, Y. (1991 “1990”) Key to Chinese Amphibia. Chongqing Branch Science and Technology Literature Press, Chongquing, 364 pp.-

    Fei, L., Ye, C. & Jiang, J. (2010) Colored Atlas of Chinese Amphibians. Sichuan Publishing Group, Sichuan Publishing House of Science & Technology, Sichuan, 519 pp.

    Frost, D.R. (2015) Amphibian species of the world: An Online Reference, version 6.0. American Museum of Natural History, New York. Available from: http://research.amnh.org/herpetology/amphibia/index.php/ (accessed 2 February 2016)

    Hillis, D.M., Frost, J.S. & Wright, D.A. (1983) Phylogeny and biogeography of the Rana pipiens complex: a biochemical evaluation. Systematic Biology, 32, 132–143.
    http://dx.doi.org/10.1093/sysbio/32.2.132

    Inger, R.F., Orlov, N. & Darevsky, I. (1999) Frogs of Vietnam: a report on new collections. Fieldiana Zoology, 92, 1–46.

    Jiang, K., Yan, F., Suwannapoom, C., Chomdej, S. & Che, J. (2013) A new species of the genus Leptolalax (Anura: Megophryidae) from northern Thailand. Asian Herpetological Research, 4, 100–108.
    http://dx.doi.org/10.3724/SP.J.1245.2013.00100

    Lathrop, A., Murphy, R.W., Orlov, N. & Ho, C.T. (1998) Two new species of Leptolalax (Anura: Megophryidae) from northern Vietnam. Amphibia-Reptilia, 19, 253–267.
    http://dx.doi.org/10.1163/156853898X00160

    Matsui, M. (1997) Call characteristics of Malaysian Leptolalax with a description of two new species (Anura: Pelobatidae). Copeia, 1997, 158–165.
    http://dx.doi.org/10.2307/1447851

    Matsui, M. (2006) Three new species of Leptolalax from Thailand (Amphibia, Anura, Megophryidae). Zoological Science, 23, 821–830.
    http://dx.doi.org/10.2108/zsj.23.821

    Matsui, M., Belabut, D.M., Ahmad, N. & Yong, H.-S. (2009) A new species of Leptolalax (Amphibia, Anura, Megophryidae) from Peninsular Malaysia. Zoological Science, 26, 243–247.
    http://dx.doi.org/10.2108/zsj.26.243

    Meyfroidt, P. & Lambin, E.F. (2008) The causes of the reforestation in Vietnam. Land Use Policy, 25 (2), 182–197.
    http://dx.doi.org/10.1016/j.landusepol.2007.06.001

    Moran, M.D. (2003) Arguments for rejecting the sequential Bonferroni in ecological studies. Oikos, 2003 403–405.
    http://dx.doi.org/10.1034/j.1600-0706.2003.12010.x

    Nakagawa, S. (2004) A farewell to Bonferroni: the problems of low statistical power and publication bias. Behavioral Ecology, 15 (6), 1044–1045.
    http://dx.doi.org/10.1093/beheco/arh107

    Ohler, A., Marquis, O., Swan, S. & Grosjean, S. (2000) Amphibian biodiversity of Hoang Lien Nature Reserve (Lao Cai Province, northern Vietnam) with description of two new species. Herpetozoa, 13, 71–87.

    Ohler, A., Wollenberg, K.C., Grosjean, S., Hendrix, R., Vences, M., Ziegler, T. & Dubois, A. (2011) Sorting out Lalos: description of new species and additional taxonomic data on megophryid frogs from northern Indochina (genus Leptolalax, Megophryidae, Anura). Zootaxa, 3147, 1–83.

    Palumbi, S. (1991) Simple fool's guide to PCR. Department of Zoology, University of Hawaii, Honolulu, HI, 45 pp.

    Perneger, T.V. (1998) What’s wrong with Bonferroni adjustments. BMJ: British Medical Journal, 316 (7139), 1236.
    http://dx.doi.org/10.1136/bmj.316.7139.1236

    Poyarkov, N.A. Jr., Rowley, J.J.L., Gogoleva, S.I., Vassilieva, A.B., Galoyan, E.A. & Orlov, N. L. (2015) A new species of Leptolalax (Anura: Megophryidae) from the western Langbian Plateau, southern Vietnam. Zootaxa, 3931 (2), 221–252.
    http://dx.doi.org/10.11646/zootaxa.3931.2.3

    Rambaut, A. & Drummond, A.J. (2007) Tracer v1. 4. Available from: http://beast.bio.ed.ac.uk/Tracer (accessed 19 January 2016)

    Rambaut, A. & Drummond, A.J. (2009) Figtree v1.3.1. Available from: http://tree.bio.ed.ac.uk/software/figtree/ (accessed 19 January 2016)

    Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539–542.
    http://dx.doi.org/10.1093/sysbio/sys029

    Rowley, J.J.L. & Cao, T.T. (2009) A new species of Leptolalax (Anura: Megophryidae) from central Vietnam. Zootaxa, 2198, 51–60.

    Rowley, J.J.L., Le, D.T.T., Tran, D.T.A. & Hoang, H.D. (2011) A new species of Leptolalax (Anura: Megophryidae) from southern Vietnam. Zootaxa, 2796, 15–28.

    Rowley, J.J.L., Stuart, B.L., Neang, T. & Emmett, D.A. (2010a) A new species of Leptolalax (Anura: Megophryidae) from northeastern Cambodia. Zootaxa, 2567, 57–68.

    Rowley, J.J.L., Stuart, B.L., Richards, S.J., Phimmachak, S. & Sivongxay, N. (2010b) A new species of Leptolalax (Anura: Megophryidae) from Laos. Zootaxa, 2681, 35–46.

    Rowley, J.J.L., Hoang, D.H., Le, T.T.D., Dau, Q.V. & Cao, T.T. (2010c) A new species of Leptolalax (Anura: Megophryidae) from Vietnam and further information on Leptolalax tuberosus. Zootaxa, 2660, 33–45.

    Rowley, J., Brown, R., Kusrini, M., Inger, R., Stuart, B., Wogan, G., Chan-ard, T., Cao, T.T., Diesmos, A., Iskandar, D.T., Lau, M., Ming, L.T., Makchai, S., Neang, T., Nguyen, Q.T. & Phimmachak, S. (2010d) Impending conservation crisis for Southeast Asian amphibians. Biology Letters, 6, 336–338
    http://dx.doi.org/10.1098/rsbl.2009.0793

    Rowley, J.J.L., Hoang, D.H., Dau, Q.V., Le, T.T.D. & Cao, T.T. (2012) A new species of Leptolalax (Anura: Megophryidae) from central Vietnam. Zootaxa, 3321, 56–68.

    Rowley, J.J.L., Dau, V.Q. & Nguyen, T.T. (2013) A new species of Leptolalax (Anura: Megophryidae) from the highest mountain in Indochina. Zootaxa, 3737 (4), 415–428.
    http://dx.doi.org/10.11646/zootaxa.3737.4.5

    Rowley, J.J.L., Stuart, B.L., Neang, T., Hoang, H.D., Dau, V.Q., Nguyen, T.T. & Emmett, D.A. (2015) A new species of Leptolalax (Anura: Megophryidae) from Vietnam and Cambodia . Zootaxa, 4039 (3), 401–417.
    http://dx.doi.org/10.11646/zootaxa.4039.3.1

    Rowley, J.J.L., Tran, D.T.A., Frankham, G.J., Dekker, A.H., Le, D.T.T., Nguyen, T.Q., Dau, V.Q. & Hoang, H.D. (2015) Undiagnosed cryptic diversity in small, microendemic frogs (Leptolalax) from the Central Highlands of Vietnam. PLoS ONE, 10 (5), e0128382.
    http://dx.doi.org/10.1371/journal.pone.0128382

    Sukumaran, J. & Holder, M.T. (2010) DendroPy: a Python library for phylogenetic computing. Bioinformatics, 26, 1569–1571.
    http://dx.doi.org/10.1093/bioinformatics/btq228

    Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA 5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28, 2731–2739.
    http://dx.doi.org/10.1093/molbev/msr121

    Wilcox, T.P., Zwickl, D.J., Heath, T.A. & Hillis, D.M. (2002) Phylogenetic relationships of the dwarf boas and a comparison of Bayesian and bootstrap measures of phylogenetic support. Molecular Phylogenetics and Evolution, 25, 361–371.
    http://dx.doi.org/10.1016/S1055-7903(02)00244-0

    Zwickl, D.J. (2006) Genetic algorithm approaches for the phylogenetic analysis of large biological sequence datasets under the maximum likelihood criterion. Ph.D. dissertation, The University of Texas at Austin. [unkown pagination]