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Issue: Vol. 4237 No. 3: 1 Mar. 2017
Type: Article
Published: 2017-03-01
DOI: 10.11646/zootaxa.4237.3.4
Page range: 517–544
Abstract views: 160
PDF downloaded: 2

Morphology, songs and genetics identify two new cicada species from Morocco: Tettigettalna afroamissa sp. nov. and Berberigetta dimelodica gen. nov. & sp. nov. (Hemiptera: Cicadettini)

Computational Biology and Population Genomics Group, Centre for Ecology, Evolution and Environmental Changes, Departamento de Biologia Animal, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal.
Computational Biology and Population Genomics Group, Centre for Ecology, Evolution and Environmental Changes, Departamento de Biologia Animal, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal.
Computational Biology and Population Genomics Group, Centre for Ecology, Evolution and Environmental Changes, Departamento de Biologia Animal, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal.
Computational Biology and Population Genomics Group, Centre for Ecology, Evolution and Environmental Changes, Departamento de Biologia Animal, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal.
Computational Biology and Population Genomics Group, Centre for Ecology, Evolution and Environmental Changes, Departamento de Biologia Animal, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal.
Computational Biology and Population Genomics Group, Centre for Ecology, Evolution and Environmental Changes, Departamento de Biologia Animal, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal.
Computational Biology and Population Genomics Group, Centre for Ecology, Evolution and Environmental Changes, Departamento de Biologia Animal, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal.
Computational Biology and Population Genomics Group, Centre for Ecology, Evolution and Environmental Changes, Departamento de Biologia Animal, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, 1749-016 Lisboa, Portugal.
Hemiptera Cicada new genus new species Morocco

Abstract

Morocco has been the subject of very few expeditions on the last century with the objective of studying small cicadas. In the summer of 2014 an expedition was carried out to Morocco to update our knowledge with acoustic recordings and genetic data of these poorly known species. We describe here two new small-sized cicadas that could not be directly assigned to any species of North African cicadas: Tettigettalna afroamissa sp. nov. and Berberigetta dimelodica gen. nov. & sp. nov. In respect to T. afroamissa it is the first species of the genus to be found outside Europe and we frame this taxon within the evolutionary history of the genus. Acoustic analysis of this species allows us to confidently separate T. afroamissa from its congeners. With B. dimelodica, a small species showing a remarkable calling song characterized by an abrupt frequency modulation, a new genus had to be erected. Bayesian inference and maximum likelihood phylogenetic analyses with DNA-barcode sequences of Cytochrome C Oxidase 1 support the monophyly of both species, their distinctness and revealed genetic structure within B. dimelodica. Alongside the descriptions we also provide GPS coordinates of collection points, distributions and habitat preferences.

 

References

  1. Boulard, M. (1980) Cigales nouvelles ou mal connues d’Afrique du Nord (Homoptera Cicadoidea). Nouvelle Revue Entomologie, 10 (42), 313–324.

    Boulard, M. (1981) Homoptères Cicadoidea récoltés en algérie par M. J.-M. Maldès. Revue Française d’Entomologie, New Series, 3 (2), 37–45.

    Boulard, M. (1987) Cigales nouvellles d’Afrique du Nord (hom. Cicadoidea). L’ Entomologiste, 43 (4), 215–218.

    Boulard, M. (2006) Acoustic signals, diversity and behaviour of cicadas. In: Drosopoulos, S. & Claridge M. (Eds.), Insect Sounds and Communication: Physiology, Behaviour, Ecology, and Evolution. CRC Press, Florida, pp. 331–349.

    Claridge, M. (1985) Acoustic signals in the Homoptera: Behavior, taxonomy, and evolution. Annual Review of Entomology, 30 (1), 297–317.
    https://doi.org/10.1146/annurev.ento.30.1.297

    Gogala, M. (1995) Songs of four cicada species from Thailand. Bioacoustics, 6 (2), 101–116.
    https://doi.org/10.1080/09524622.1995.9753278

    Gogala, M. & Trilar, T. (2004) Biodiversity of cicadas in Malaysia—A bioacoustic approach. Serangga, 9 (1–2), 63–81.

    Gogala, M., Trilar, T., Kozina, U. & Duffels, H. (2004) Frequency modulated song of the cicada Maua albigutta (Walker, 1856) (Auchenorrhyncha: Cicadoidea) from Southeast Asia. Scopolia, 54, 1–15.

    Hajibabaei, M., Smith, M.A., Janzen, D.H., Rodriguez, J.J., Whitfield, J.B. & Hebert, P.D.N. (2006) A minimalist barcode can identify a specimen whose DNA is degraded. Molecular Ecology Notes, 6 (4), 959–964.
    https://doi.org/10.1111/j.1471-8286.2006.01470.x

    Hall, T.A. (1999) Bioedit: A user-friendly biological sequence alignment editor and analysis program for windows 95/98/nt. Nucleic Acids Symposium Series, 41, 95–98.
    https://doi.org/citeulike-article-id:691774

    Hausdorf, B. (2011) Progress toward a general species concept. Evolution, 65 (4), 923–931.
    https://doi.org/10.1111/j.1558-5646.2011.01231.x

    Hebert, P.D.N., Penton, E.H., Burns, J.M., Janzen, D.H. & Hallwachs, W. (2004) Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences of the United States of America, 101 (41), 14812–14817.
    https://doi.org/10.1073/pnas.0406166101

    Hebert, P.D.N., Ratnasingham, S. & de Waard, J.R. (2003) Barcoding animal life: Cytochrome C Oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society B: Biological Sciences, 270 (Supplement 1), S96–S99.
    https://doi.org/10.1098/rsbl.2003.0025

    Hertach, T., Puissant, S., Gogala, M., Trilar, T., Hagmann, R., Baur, H., Kunz, G., Wade, E.J., Loader, S.P., Simon, C. & Nagel, P. (2016) Complex within a complex: integrative taxonomy reveals hidden diversity in Cicadetta brevipennis (Hemiptera: Cicadidae) and unexpected relationships with a song divergent relative. PLOS ONE, 11 (11), e0165562.
    https://doi.org/10.1371/journal.pone.0165562

    Hertach, T., Trilar, T., Wade, E.J., Simon, C. & Nagel, P. (2015) Songs, genetics, and morphology: revealing the taxonomic units in the European Cicadetta cerdaniensis cicada group, with a description of new taxa (Hemiptera: Cicadidae). Zoological Journal of the Linnean Society, 173 (2), 320–351.
    https://doi.org/10.1111/zoj.12212

    Katoh, K. & Standley, D.M. (2013) MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution, 30 (4), 772–780.
    https://doi.org/10.1093/molbev/mst010

    Linares, M.C., Soto-Calderón, I.D., Lees, D.C. & Anthony, N.M. (2009) High mitochondrial diversity in geographically widespread butterflies of Madagascar: a test of the DNA barcoding approach. Molecular Phylogenetics and Evolution, 50 (3), 485–495.
    https://doi.org/10.1016/j.ympev.2008.11.008

    Marshall, D.C., Hill, K.B.R., Moulds, M., Vanderpool, D., Cooley, J.R., Mohagan, A.B. & Simon, C. (2015) Inflation of molecular clock rates and dates: molecular phylogenetics, biogeography, and diversification of a global cicada radiation from Australasia (Hemiptera: Cicadidae: Cicadettini). Systematic Biology, 65 (1), 16–34.
    https://doi.org/10.1093/sysbio/syv069

    Mayr, E. (1963) Animal Species and Evolution. Belknap Press of Harvard University, Cambridge, 797 pp.

    Mendes, R., Nunes, V.L., Quartau, J. & Simões, P.C. (2014) Patterns of acoustic and morphometric variation in species of genus Tettigettalna (Hemiptera: Cicadidae): sympatric populations show unexpected differences. European Journal of Entomology, 111 (3), 429–441.
    https://doi.org/10.14411/eje.2014.054

    Moulds, M.S. (2005) An appraisal of the higher classification of cicadas (Hemiptera: Cicadoidea) with special reference to the Australian fauna. Records of the Australian Museum, 57 (3), 375–446.
    https://doi.org/10.3853/j.0067-1975.57.2005.1447

    Moulds, M.S. (2012) A Review of the Genera of Australian Cicadas (Hemiptera: Cicadoidea). Zootaxa, 3287, 1–262.

    Nosil, P. (2008) Speciation with gene flow could be common. Molecular Ecology, 17 (9), 2103–2106.
    https://doi.org/10.1111/j.1365-294X.2008.03715.x

    Nunes, V.L., Mendes, R., Marabuto, E., Novais, B.M., Hertach, T., Quartau, J., Seabra, S.G., Paulo, O.S. & Simões, P.C. (2014a) Conflicting patterns of DNA barcoding and taxonomy in the cicada genus Tettigettalna from southern Europe (Hemiptera: Cicadidae). Molecular Ecology Resources, 14, 27–38.
    https://doi.org/10.1111/1755-0998.12158

    Nunes, V.L., Mendes, R., Quartau, J. & Simões, P.C. (2014)b) Current distribution raises concerns on the conservation of Tettigettalna mariae (Quartau & Boulard, 1995) (Hemiptera: Cicadoidea) in portugal. Ecologi@, 7, 50–57.

    Nylander, J., Ronquist, F., Huelsenbeck, J. & Nieves-Aldrey, J.L. (2004) Bayesian phylogenetic analysis of combined data. Systematic Biology, 53 (1), 47–67.
    https://doi.org/10.1080/10635150490264699

    Pina-Martins, F. & Paulo, O.S. (2008) Concatenator: sequence data matrices handling made easy. Molecular Ecology Resources, 8 (6), 1254–1255.

    https://doi.org/10.1111/j.1755-0998.2008.02164.x

    Pinto-Juma, G., Simões, P.C., Seabra, S.G. & Quartau, J. (2005) Calling song structure and geographic variation in Cicada orni linnaeus (Hemiptera: Cicadidae). Zoological Studies, 44 (1), 81–94.

    Puissant, S. & Sueur, J. (2010) A hotspot for mediterranean cicadas (Insecta: Hemiptera: Cicadidae): new genera, species and songs from southern Spain. Systematics and Biodiversity, 8 (4), 555–574.

    https://doi.org/10.1080/14772000.2010.532832

    Quartau, J. & Simões, P. (2006) Acoustic evolutionary divergence in cicadas: the species of Cicada L. in southern Europe. Insect Sounds and Communication:Physiology, Behaviour, Ecology, and Evolution, Chapter 7, 227–237.

    De Queiroz, K. (2007) Species concepts and species delimitation. Systematic Biology, 56 (6), 879–886.
    https://doi.org/10.1080/10635150701701083

    De Queiroz, K. (2016) Ernst Mayr and the modern concept of species. Proceedings of the National Academy of Sciences, 102, 6600–6607.
    https://doi.org/10.1073/pnas.0502030102

    Ronquist, F., Teslenko, M., Van Der Mark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61 (3), 539–542.
    https://doi.org/10.1093/sysbio/sys029

    Sanborn, A.F. (2014) Catalogue of the Cicadoidea (Hemiptera: Auchenorrhyncha). Academic Press, San Diego, California, 1010 pp.

    Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Rueden, C., Saalfeld, S., Schmid, B., Tinevez, J.-Y., White, D.J., Hartenstein, V., Eliceiri, K., Tomancak, P. & Cardona, A. (2012) Fiji: an open-source platform for biological-image analysis. Nature Methods, 9 (7), 676–682.
    https://doi.org/10.1038/nmeth.2019

    Schmitt, T. (2007) Molecular biogeography of Europe: Pleistocene cycles and postglacial trends. Frontiers in Zoology, 4 (11), 1–13.
    https://doi.org/10.1186/1742-9994-4-11

    Simões, P., Nunes, V., Mendes, R. & Quartau, J. (2013) First record of tettigettalna mariae quartau & boulard, 1995 (Insecta: Hemiptera: Cicadoidea) in Spain. Biodiversity Data Journal, 1, e978.
    https://doi.org/10.3897/BDJ.1.e978

    Simões, P. & Quartau, J. (2006) Selective responsiveness in males of Cicada orni to conspecific and allospecific calling songs (Hemiptera: Cicadidae). Entomologia generalis, 29 (1), 047–060.

    Simões, P.C., Boulard, M., Rebelo, M.T., Drosopoulos, S., Claridge, M.F., Morgan, J.C. & Quartau, J. (2000) Differences in the male calling songs of two sibling species of Cicada (Hemiptera: Cicadoidea) in Greece. European Journal of Entomology, 97 (3), 437–440.
    https://doi.org/10.14411/eje.2000.067

    Simões, P.C., Nunes, V.L., Mendes, R., Seabra, S.G. & Paulo, O.S. (2014) Tettigettalna josei (Boulard, 1982) (Hemiptera: Cicadoidea): first record in Spain, with notes on the distribution, genetic variation and behaviour of the species. Biodiversity Data Journal, 2, e1045.
    https://doi.org/10.3897/BDJ.2.e1045

    Specht, R. (2004) Avisoft -SASLab Pro. Sound analysis and synthesis laboratory.

    Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30 (9), 1312–1313.
    https://doi.org/10.1093/bioinformatics/btu033

    Sueur, J. & Puissant, S. (2007) Similar look but different song: a new Cicadetta species in the montana complex (Insecta, Hemiptera, Cicadidae). Zootaxa, 68, 55–68.

    Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) Mega6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30 (12), 2725–2729.
    https://doi.org/10.1093/molbev/mst197

    Trilar, T. (2006) Frequency modulated song of the cicada Kalabita operculata (Auchenorrhyncha: Cicadoidea) from Borneo. Russian Entomological Journal, 15 (3), 341–346.

    Villiers, A. (1943) Révision des adeniana du nord de l’Afrique (Hemiptera: Cicadidae). Bulletin du Muséum national d’Histoire Naturelle, 6, 424–428.

    Wiemers, M. & Fiedler, K. (2007) Does the DNA barcoding gap exist?—a case study in blue butterflies (Lepidoptera: Lycaenidae). Frontiers in Zoology, 4 (8), 1–16.
    https://doi.org/10.1186/1742-9994-4-8