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Type: Monograph
Published: 2018-12-21
Page range: 1–75
Abstract views: 386
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Review of the genus Cobitis in the Middle East, with the description of eight new species (Teleostei: Cobitidae)

Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), 12587 Berlin, Germany.
Recep Tayyip Erdogan University, Faculty of Fisheries and Aquatic Sciences, 53100 Rize, Turkey.
Zoological Research Museum Alexander Koenig, Leibniz Institute for Animal Biodiversity, Adenauerallee 160, 53113 Bonn, Germany.
Pisces Freshwater fish Taxonomy Cytochrome oxidase I Middle East

Abstract

The diversity of Cobitis in the Middle East is reviewed, resulting in the recognition of 30 species, of which eight are described herein as new. Two species, C. amphilekta and C. kellei, seem to be extinct. Hypotheses on species-level diversity derived from distance and Poisson tree process analyses of DNA barcode data are tested against morphometric and morphological characters including colour patterns. For species pairs separated by small K2P distances in COI sequence data we follow a practitioner-oriented diagnostic species concept, in which we recognise species only if differentiated morphologically (including by colour pattern). For all 30 species we provide diagnoses and identification keys. Cobitis afifeae, new species, from the Büyük Menderes River drainage in the Aegean Sea basin, is distinguished by having two laminae circularis in the male, a row of blotches below Z4, a small, roundish or comma-shaped black spot at the upper caudal-fin base, and elevated mental lobes. Cobitis aliyeae, new species, from the lower Seyhan and Ceyhan River drainages, is distinguished by having two laminae circularis in the male, the blotches in Z2 and Z4 anterior to the dorsal-fin origin usually well separated from each other, and the pigmentation in Z1 well distinguished from the pigmentation in Z2. Cobitis anabelae, new species, from the lower Orontes River drainage, is distinguished by having two laminae circularis in the male, the pigmentation in Z2 formed by small, brown spots, always much smaller than blotches in Z3, much smaller than the pupil diameter, Z2 and Z3 well separated, and no pigmentation below Z4. Cobitis erkakanae, new species, from the Gölbasi Lakes, adjacent to the Ceyhan River drainage, is distinguished by having two laminae circularis in the male, no blotches below Z4, the blotches in Z2 and Z4 being horizontally elongated and often fused with adjacent ones, and the caudal fin with 46 wide, regularly-shaped, brown bands. Cobitis emrei, new species, from the Lake Sapanca basin is distinguished by having one lamina circularis in the male, a large black spot at the upper caudal-fin base, and Z3 fully covered by very small spots forming a sand-like pattern. Cobitis joergbohleni, new species, from the Sultan marshes in Central Anatolia is distinguished by having two laminae circularis in the male, and the flank colour pattern being completely disorganised, not following the Gambetta zones. Cobitis pirii, new species, from the endorheic Lake Eğirdir basin and the Mediterranean Aksu and Köprü Rivers, is distinguished by having two laminae circularis in the male, a simple external part of the suborbital spine and two distinct rows of small blotches in Z4, one along the lateral midline and one distinctly below. Cobitis troasensis, new species, from the Tuzla River drainage, is distinguished by having one lamina circularis in the male and 25–36 small, comma-shaped brown blotches in Z4. A lectotype is designated for Cobitis battalgilae. As First Revisers, priority is given to Cobitis fahireae over C. kurui. Cobitis damlae and C. kurui are treated as synonyms of C. fahireae. Cobitis strumicae and C. taenia are recorded for the first time from Anatolia and C. saniae is newly documented from the Black Sea basin in Georgia. The Poisson tree process analysis of COI data proposed 31 groups, most of which could be distinguished by morphological characters. Cobitis troasensis is described based on morphological data alone.

 

 

References

  1. Băcescu, M. (1962) Contribution à la systématique du genre Cobitis. Description d’une espèce nouvelle, Cobitis calderoni, provenant de l’Espagne. Revue de Biologie, Académie de la République Populaire Roumaine, Bucarest,6 (4), 435–448.

    Coad, B.W. (2010) Freshwater Fishes of Iraq. Pensoſt Publishers, Sofia-Moscow, 294 pp.

    Coad, B.W. & Sarieyyüpoğlu, M. (1988) Cobitis elazigensis, a new species of cobitidid fish from Anatolia, Turkey. Japanese Journal of Ichthyology, 34 (4), 426–430.

    https://doi.org/10.1007/BF02905648

    Biomatters (2013) Geneious Pro. Available from: http://www.geneious.com (accessed 15 August 2018)

    Bohlen, J., Perdices, A., Doadrio, I. & Economidis, P.S. (2006) Vicariance, colonisation, and fast local speciation in Asia Minor and the Balkans as revealed from the phylogeny of spined loaches (Osteichthyes; Cobitidae). Molecular Phylogenetics and Evolution, 39, 552–561.

    https://doi.org/10.1016/j.ympev.2005.12.007

    Eagderi, S., Jouladeh-Roudbar, A., Jalili, P. , Sayyadzadeh G. &. Esmaeili, H.R. (2017a) Taxonomic status of the genus Cobitis Linnaeus, 1758 (Teleostei: Cobitidae) in the southern Caspian Sea basin, Iran with description of a new species. FishTaxa, 2 (1), 48–61.

    Eagderi, S., Jouladeh-Roudbar, A., Nasri, M., Sayyadzadeh, G. & Esmaeili, H.R. (2017b) Taxonomic status of the genus Cobitis (Teleostei: Cobitidae) in the Namak Lake basin, Iran. Iranian Journal of Ichthyology, 4, 131–139.

    Economidis, P.S. & Nalbant, T.T. (1996) A study of the loaches of the genera Cobitis and Sabanejewia (Pisces, Cobitidae) of Greece, with description of six new taxa. Travaux du Muséum d'Histoire Naturelle "Grigore Antipa", 36, 295–347

    Edgar, R.C. (2004) MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic acids research, 32, 1792–1797.

    https://doi.org/10.1093/nar/gkh340

    Erk'akan, F., Atalay-Ekmekçi, F.G. &Nalbant, T.T. (1998) Four new species and one new subspecies of the genus Cobitis (Pisces: Ostariophysi: Cobitidae) from Turkey. Turkish Journal of Zoology, 22, 9–15.

    Erk'akan, F., Atalay-Ekmekçi, F.G. & Nalbant, T.T. (1999) A review of the genus Cobitis in Turkey (Pisces: Ostariophysi: Cobitidae). Hydrobiologia, 403, 13–26.

    https://doi.org/10.1023/A:1003794726444

    Erk'akan, F., Özeren, S.C. & Nalbant, T.T. (2008) Cobitis evreni sp. Nova-A new spined loach species (Cobitidae) from southern Turkey. Journal of Fisheries International, 3 (4), 112–114. [electronic journal]

    Erk'akan, F. & Özdemir, F. (2014) The first new cave fish species, Cobitis damlae (Teleostei: Cobitidae) from Turkey. Hacettepe Journal of Biology and Chemistry, 42 (2), 275–279.

    https://doi.org/10.15671/HJBC.20144210863

    Erk'akan, F., Özdemir, F. & Özeren, S.C. (2017) Two new species of the genus Cobitis Linnaeus (Teleostei: Cobitidae) from Turkey. FishTaxa, 2 (2), 82–89.

    Freyhof, J., Ekmekçi, F.G., Ali, A., Khamees, N.R., Özuluğ, M., Hamidan, N., Küçük, F. & Smith, K.G. (2014) Chapter 3. Freshwater fishes. In: Smith, K.G., Barrios, V., Darwall, W.R.T. & Numa, C. (Eds.), The status and distribution of freshwater biodiversity in the Eastern Mediterranean. IUCN, Cambridge, Malaga and Gland, pp. 19–42.

    https://doi.org/10.2305/IUCN.CH.2014.01.en

    Freyhof, J., Kaya, C., Bayçelebi, E., Geiger, M. & Turan, D. (2018) Generic assignment of Leuciscus kurui Bogutskaya from the upper Tigris drainage, and a replacement name for Alburnus kurui Mangit & Yerli (Teleostei: Leuciscidae). Zootaxa, 4410 (1), 113–135.

    https://doi.org/10.11646/zootaxa.4410.1.6

    Freyhof, J., Stelbrink, B., Özuluğ, M. & Economidis, P.S. (2008) The first record of Cobitis puncticulata from Europe with comments on its conservation status (Teleostei: Cobitidae). Folia Zoologica, 57, 16–19.

    Gambetta, L. (1934). Sulla variabilita del cobite fluviale (Cobitis taenia L.) e sul rapporto numerico dei sessi. Bollettino dei Musei di Zoologia e di Anatomia Comparata della Reale Università di Torino, 44, 297–324.

    Geiger, M.F., Herder, F., Monaghan, M.T., Almada, V., Barbieri, R., Bariche, M., Berrebi, P., Bohlen, J., Casal-Lopez, M., Delmastro, G.B., Denys, G.P.J., Dettai, A., Doadrio, I., Kalogianni, E., Kärst, H., Kottelat, M., Kovačić, M., Laporte, M., Lorenzoni, M., Marčić, Z., Özuluğ, M., Perdices, A., Perea, S., Persat, H., Porcelotti, S., Puzzi, C., Robalo, J., Šanda, R., Schneider, M., Šlechtová, V., Stoumboudi, M., Walter, S. & Freyhof, J. (2014) Spatial heterogeneity in the Mediterranean Biodiversity Hotspot affects barcoding accuracy of its freshwater fishes. Molecular Ecology Resources, 14, 1210–1221.

    https://doi.org/10.1111/1755-0998.12257

    Güçlü, S.S. & Küçük, F. (2015) The Ichthyofauna of Gediz River (Turkey): Taxonomic and zoogeographic features. Annual Research & Review in Biology, 6 (3), 202–214.

    https://doi.org/10.9734/ARRB/2015/14889

    Hankó, B. (1924) Fische aus Klein-Asien. Annales Historico-Naturales Musei Nationalis Hungarici, 21,137–158, pl. 3.

    ICZN (International Code of Zoological Nomenclature) (1999) International Code of Zoological Nomenclature. 4th Edition. International Trust for Zoological Nomeclature, London, 306 pp.

    İlhan, A. (2006) Batı Karadeniz Bölgesi tatlisu baliklarinin taksonomik ve ekolojik özelliklerinin araştırılması. Doktora tezi, Ege Üniversitesi Fen Bilimleri Enstitüsü, İzmir, 164 pp. [in Turkish]

    Ivanova, N.V., Zemlak, T.S., Hanner, R.H. & Hebert, P.D.N. (2007) Universal primer cocktails for fish DNA barcoding. Molecular Ecology Notes, 7, 544–548.

    https://doi.org/10.1111/j.1471-8286.2007.01748.x

    Janko, K., Bohlen, J., Lamatsch, D., Flajšhans, M., Epplen, J.T., Ráb, P., Kotlík, P. & Šlechtová, V. (2007a) The gynogenetic reproduction of diploid and triploid hybrid spined loaches (Cobitis: Teleostei), and their ability to establish successful clonal lineages—on the evolution of polyploidy in asexual vertebrates. Genetica, 131, 185–194.

    https://doi.org/10.1007/s10709-006-9130-5

    Janko, K., Flajšhans, M., Choleva, L., Bohlen, J., Šlechtová, V.,Rábová, M., Lajbner, Z., Šlechta, V., Ivanova, P., Dobrovolov, I., Culling, M., Persat, H., Kotusz, J. & Ráb, P. (2007b) Diversity of European spined loaches (genus Cobitis L.): an update of the geographic distribution of the Cobitis taenia hybrid complex with a description of new molecular tools for species and hybrid determination. Journal of Fish Biology, 71 (Supplement C), 387–408.

    https://doi.org/10.1111/j.1095-8649.2007.01663.x

    Janko, K., Vasil'ev, V.P., Ráb, P., Rábová, M., Šlechtová, V. & Vasil'eva, E.D. (2005) Genetic and morphological analyses of 50-chromosome spined loaches (Cobitis, Cobitidae, Pisces) from the Black Sea basin that are morphologically similar to C. taenia, with the description of a new species. Folia Zoologica, 54, 405–420.

    Jouladeh-Roudbar, A., Eagderi, S., Sayyadzadeh, G. & Esmaeili, H.R. (2017) Cobitis keyvani, a junior synonym of Cobitis faridpaki (Teleostei: Cobitidae). Zootaxa, 4244 (1), 118 –126.

    https://doi.org/10.11646/zootaxa.4244.1.6

    Kapli, P., Lutteropp, S., Zhang, J., Kobert, K., Pavlidis, P., Stamatakis A. & Flouri, T. (2017) Multi-rate Poisson Tree Processes for single-locus species delimitation under Maximum Likelihood and Markov Chain Monte Carlo. Bioinformatics, 33 (11), 1630–1638. https://doi.org/10.1093/bioinformatics/btx025

    Kottelat, M. & Freyhof, J. (2007) Handbook of European freshwater fishes. Kottelat, Cornol and Freyhof, Berlin, xiv + 646 pp.

    Kottelat, M. (2012) Conspectus Cobitidum: an inventory of the loaches of the world (Teleostei: Cypriniformes: Cobitoidei). Raffles Bulletin of Zoology, 26 (Supplement), 1–199.

    Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33, 1870–1874.

    https://doi.org/10.1093/molbev/msw054

    Krupp, F. (1985) Systematik und Zoogeographie der Süsswasserfische des levantinischen Grabenbruchsystems und der Ostküste des Mittelmeers. Dissertation, Johannes Gutenberg Universität, Mainz, 215 pp.

    Krupp, F. & Moubayed, J. (1992) A new species of Cobitis Linnaeus 1758 from the Orontes and Litani drainage basins of Syria and Lebanon (Pisces: Osteichthyes: Cobitidae). Senckenbergiana Biologica, 72, 13–18.

    Ludwig, A., Bohlen, J., Wolter, C. & Pitra, C. (2001) Phylogenetic relationships and historial biogeography of spined loaches (Cobitidae, Cobitis and Sabanejewia) as indicated by variability of mitochondrial DNA. Zoological Journal of the Linnean Society, 131, 381–392.

    https://doi.org/10.1111/j.1096-3642.2001.tb02242.x

    Masters, B.C., Fan, V. & Ross, H.A. (2011) Species delimitation–a geneious plugin for the exploration of species boundaries. Molecular Ecology Resources, 11, 154–157.

    https://doi.org/10.1111/j.1755-0998.2010.02896.x

    Mousavi-Sabet, H., Vasil'eva, E.D., Vatandoust, S. & Vasil'ev, V.P. (2011) Cobitis faridpaki sp. nova—a new spined loach species (Cobitidae) from the southern Caspian Sea basin. Journal of Ichthyology, 51 (10), 925–931.

    https://doi.org/10.1134/S0032945211100055

    Mousavi-Sabet, H., Yerli, S.V., Vatandoust, S., Özeren, S.C. & Moradkhani, Z. (2012) Cobitis keyvani sp. nova—a new species of spined-loach from south of the Caspian Sea (Teleostei: Cobitidae). Turkish Journal of Fisheries and Aquatic Sciences, 12, 7–13.

    Mousavi-Sabet, H., Vatandoust, S., Esmaeili, H.R., Geiger, M.F. & Freyhof, J. (2015) Cobitis avicennae, a new species of spined loach from the Tigris River drainage (Teleostei: Cobitidae). Zootaxa, 3914 (5), 558–568.

    https://doi.org/10.11646/zootaxa.3914.5.4

    Özuluğ, M. & Freyhof, J. (2011) Revision of the genus Squalius in western and central Anatolia, with description of four new species (Teleostei: Cyprinidae). Ichthyological Exploration of Freshwaters, 22, 107–148.

    Perdices, A., Özeren, C.S., Erk'akan, F. & Freyhof, J. (2018) Diversity of spined loaches from Asia Minor in a phylogenetic context (Teleostei: Cobitidae). PLoS ONE, 13 (10), e0205678.

    https://doi.org/10.1371/journal.pone.0205678

    Proudlove, G. (2003) Subterranean fishes of the world: An account of the subterranean (hypogean) fishes described up to 2003 with a bibliography 1541-2004. International Society for Subterranean Biology, Moulis, 300 pp.

    Saitou, N. & Nei, M. (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4 (4), 406–425.

    Swofford, D.L. (2002) PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4. Sinauer Associates, Sunderland. MA. [software]

    Tamura, K. & Nei, M. (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10, 512–526.

    https://doi.org/10.1093/oxfordjournals.molbev.a040023

    Van Neer, W., Wildekamp, R.H., Küçük, F. & Ünlüsayın, M. (2008) The 1997-1999 surveys of the Anatolian fish fauna and their relevance to the interpretation of trade at Sagalassos. In: Degryse, P. & Waelkens, M. (Eds.), Geo-and Bio- Archeology at Sagalassos and in its Territory. Leuven University Press, Leuven, pp. 299–323.

    Vasil'eva, E.D. & Vasil'ev, V.P. (2006). Cobitis pontica sp. nova - a new spined loach species (Cobitidae) from the Bulgarian Waters. Journal of Ichthyology, 46 (1),15–20.

    https://doi.org/10.1134/S003294520610002X

    Vasil'eva, E.D. & Vasil'ev, V.P. (2012) Cobitis amphilekta sp. nova, a new species of spined loaches (Cobitidae, Cypriniformes) from the Caspian Sea basin. Journal of Ichthyology, 52 (3), 200–206. [appeared in Russian in Voprosy Ikhtiologii, 52 (2),177–183 (2012)]

    https://doi.org/10.1134/S0032945212020154

    Wilkens, H. & Dohse, R. (1993) Die Typen der Ichthyologischen Sammlung des Zoologischen Instituts und Zoologischen Museums der Universität Hamburg (ZMH) Teil IV. Mitteilungen aus dem Hamburgischen Zoologischen Museum und Institut, 90, 401–426.

    Yoğurtçuoğlu, B. & Freyhof, J. (2018) Aphanius irregularis, a new killifish from south-western Anatolia (Cyprinodontiformes: Aphaniidae). Zootaxa, 4410 (2), 319–330.

    http://dx.doi.org/10.11646/zootaxa.4410.2.4

    Zhang, J., Kapli, P., Pavlidis, P. & Stamatakis, A. (2013) A general species delimitation method with applications to phylogenetic placements. Bioinformatics, 29 (22), 2869–2876.

    https://doi.org/10.1093/bioinformatics/btt499