Abstract
This study successfully documents, for the first time, the entire life cycle of Aenetus djernaesae Simonsen, 2018 and confirms the efficacy of using supplemental sources of fungi to feed the early instar larvae. Fresh cut pieces of the commercial mushroom Agaricus bisporus (J.E. Lange) and sections of Eucalyptus L’Her. bark were placed around the base of potted host plants –Myoporum insulare R.Br. (Scrophulariaceae) and the potential host plant Dodonea hackettiana W.Fitz. (Sapindaceae). First instar larvae were added to this matrix where they fed on the mushroom and bark. The life cycle comprised egg development of 20 days, fungal feeding of ~36 days, and host plant development (including pupal) of ~300 days. Adult emergence of reared and field collected samples occurred within a 22 day period. Larvae transferring from fungi to host plants transitioned during the night by constructing a web of silk and plant tissues within two hours and proceeding to excavate a tunnel from within. The mature larval tunnel is relatively short, up to 220 mm in length and usually extending below the entrance around which the larvae grazes on callus tissue forming after bark removal. Most adults emerged within an hour of dusk with the pupa protruding from the top of the vestibule. The rearing method described here demonstrates the feasibility of laboratory based studies of larval development in Aenetus Herrrich-Schäffer and other callus-feeding stem boring Hepialidae.
Key words: Hepialidae, Aenetus, life cycle, artificial diet, Myoporum, Dodonea, larval foodplant
References
Beaver E. & Grehan J. R. 2018. A simple rearing technique for larvae and observations on the larval biology of Australian ‘splendid ghost moths’, Aenetus (Herrich-Schäffer, 1855) (Lepidoptera: Hepialidae). Journal of Insect Biodiversity 8: 43–51.
https://doi.org/10.12976/jib/2018.08.3.1
Beaver E. 2019. A new species of Aenetus Herrich-Schäffer (Lepidoptera: Hepialidae) from Tasmania, with notes on the splendens group of species in South Australia. Australian Entomologist 46: 125–138.
Boudinot J. 1991. Biologie d’Aenetus cohici Viette (Lepidoptera: Hepialidae). Mémoir Museum National Histoire Naturelle 149: 167–175.
Bruch J. & Freeman M. 2017. Migration of limesand dunes in Western Australia and their impacts. Department of Mines and Petroleum, Perth, Western Australia, report, 2017.
Common I. F. B. 1970. Lepidoptera (moths and butterflies), pp. 765–866. In: The Insects of Australia. A Textbook for Students and Research Workers, Melbourne: Melbourne University Press.
Common I. F. B. 1990. Hepialoidea, pp.140–150. In: Moths of Australia. Melbourne: Melbourne University Press.
FloraBase. 2019. https://florabase.dpaw.wa.gov.au/
Froggatt W. W. 1907. Australian Insects. William Brooks Co. Ltd., Sydney, 449 pp.
Gosset C. H. 1878. Notes on the life history of Charagia virescens. Transactions and Proceedings of the New Zealand Institute 11: 345–346.
Grehan J. R. 1979. Larvae of Aenetus virescens (Lepidoptera: Hepialidae) in decaying wood. New Zealand Journal of Zoology 8: 505–514.
https://doi.org/10.1080/03014223.1979.10428400Grehan J. R. 1981. Morphological changes in the three-phase development of Aenetus virescens larvae (Lepidoptera: Hepialidae). New Zealand Journal of Zoology 6: 583–586.
https://doi.org/10.1080/03014223.1981.10427975
Grehan J. R. 1984. The host range of Aenetus virescens (Lepidoptera: Hepialidae) and its evolution. New Zealand Entomologist 8: 51–61.
Grehan J. R. 1988a. Evolution of arboreal tunneling by the larvae of Aenetus (Lepidoptera: Hepialidae). New Zealand Journal of Zoology 14: 441–462.
https://doi.org/10.1080/03014223.1987.10423019
Grehan J. R. 1988b. Life cycle of the wood-borer Aenetus virescens (Lepidoptera: Hepialidae). New Zealand Journal of Zoology 14: 209–217.
https://doi.org/10.1080/03014223.1987.10422991
Grehan J. R. 1989. Larval feeding habits of the Hepialidae. Journal of Natural History 33: 469–485.
Grehan J. R. & Care C. 2018. Recent observations on the entry of trees by larvae of the puriri moth, Aenetus virescens (Lepidoptera: Hepialidae). The Weta 52: 61–64.
Grehan J. R. & Beaver E. 2019. Novel approach to rearing callus feeding larvae of Aenetus Herrich-Schaffer, 1855 in Australia (Lepidoptera: Hepialidae). News of the Lepidoperists’ Society 1: 82–83, 85.
Grehan J. R., Mielke C. G. C., Nielsen J. & Turner J. R. G. in press. World catalog of Hepialidae. Lepidopterorum Catalogus, New Series.
Grehan J. R., Witt T. J. & Ignatyev N. 2018. New Species of Aenetus from Sumatra, Indonesia (Lepidoptera: Hepialidae) and a 5,000 km biogeographic disjunction. Entomofauna 39: 849–862.
Hudson G. V. 1885. Life history of Charagia virescens. Entomologist 18: 30–36.
Hudson G. V. 1892. An Elementary Manual of New Zealand Entomology. West, Newman & Co., London, 128 pp.
Hudson G. V. 1894. Notes on Hepialus virescens and other early insects in New Zealand. Entomologist’s Monthly Magazine 30: 11.
Hudson G. V. 1898. New Zealand Moths and Butterflies. West, Newman & Co. Ltd., London. 144 pp.
Hudson G. V. 1900. Entomology in New Zealand. The Entomologist 33: 186–189.
Hudson G. V. 1928. The Butterflies and Moths of New Zealand. Ferguson & Osborn, Wellington. 144 pp.
Illidge R. & Quail A. 1901. Australasian wood boring Hepialidae. Proceedings of the Royal Society of Queensland 16: 65–72.
Kallies A., Marriott P. & Hewish M. 2015. Moths of Victoria 6. Ghost moths, Hepialidae and Allies. Melbourne, Entomological Society of Victoria, 36 pp & CD.
King C. 1900. Life history notes on moths of the genus Aenetus (Lepidoptera, Hepialidae). Queensland Naturalist 16: 102–103.
Moeseneder C. & Hutchinson P. M. 2016. Navigator, a new endemic genus of Cetoniinae (Coleoptera: Scarabaeidae) from Australia, with descriptions of two new species and behavioural studies. Zootaxa 4173: 530–556.
https://doi.org/10.11646/zootaxa.4173.6.2
Quail A. 1899. A fragmentary paper on the larval structure etc. of Hepialus? virescens (D’bld) of New Zealand. Proceedings of the Royal Society of Queensland 15: 89–93.
Quail A. 1900a. Entomology in New Zealand. The Entomologist 33: 5–9.
Quail A. 1900b. Life histories in the hepialid group of Lepidoptera with descriptions of one new species and notes on imaginal structures. Transactions of the Entomological Society of London 1900: 411–432.
https://doi.org/10.1111/j.1365-2311.1900.tb01001.x
Quail A. 1902. On Charagia virescens Dbld. Transactions and Proceedings of the New Zealand Institute 35: 249–255.
Salesne T. 2010. À la recherche de l’Hépiale calédonienne. Insectes 159: 9–13.
Scott A. W. 1869. On the genus Charagia of Walker. Transactions of the Entomological Society of New South Wales 2: 25–35.
Scott A. W. 1864. Australian Lepidoptera and their transformations, drawn from the life by Harriet and Helena Scott. Vol. 1. London: John van Voorst, 30 pp.
https://doi.org/10.5962/bhl.title.51826
Scott A. W. 1890. Australian Lepidoptera and their transformations. Vol 2. Sydney: Australian Museum. 36 pp.
Simonsen T. J. 2018. Splendid ghost moths and their allies. A revision of Australian Abantiades, Oncopera, Aenetus and Zelotypia. Monographs of Australian Lepidoptera 12: 1–312.
https://doi.org/10.1071/9781486307487
Slashchevskiy P. [I] 1929. Zur Biologie von Phassus shamyl Chr. (Lepidoptera, Hepialidae). Revue Russe d’Entomologie 23: 189–199.
Yule K. J. & Burns K. C. 2013. Drivers of aggregation in a novel arboreal parasite: the influence of host size and infra-populations. International Journal of Parasitology 45: 195–202.
https://doi.org/10.1016/j.ijpara.2014.10.007
Yule K. J. & Burns K. C. 2017a. Adaptive advantages of appearance: predation, thermoregulation, and color of webbing built by New Zealand’s largest moth. Ecology 98: 1324–1333.
https://doi.org/10.1002/ecy.1787
Yule K. J. & Burns K. C. 2017b. Host defense predicts host specificity in a long-lived arboreal parasite. Evolutionary Ecology 31: 37–50.