Abstract
A new species of genus Bothaella (Diptera: Culicidae) collected along with two other species of the genus during surveys for flavivirus isolations in the Cuc Phuong National Park in northern Vietnam is formally described and named as Bothaella manhi, sp. n. The adults, pupa and fourth-instar larva are characterized, the male genitalia and the two immature stages are illustrated and DNA sequence data are included for regions coding for sections of the COI and COII genes (mtDNA). The species is compared and distinguished from the other species of the genus, and sequence data are used to hypothesise its phylogenetic relationship with Bo. helenae and Bo. kleini, the other two species collected during the survey.References
Cook, S., Bennett, S.N., Holmes, E.C., De Chesse, R., Moureau, G. & de Lamballerie, X. (2006) Isolation of a new strain of the flavivirus cell fusing agent virus in a natural mosquito population from Puerto Rico. Journal of General Virology, 87, 735–748.
Cook, S., Diallo, M., Sall, A.A., Cooper, A. & Holmes, E.C. (2005) Mitochondrial markers for molecular identification of Aedes mosquitoes (Diptera: Culicidae) involved in transmission of arboviral disease in West Africa. Journal of Medical Entomology, 42, 19–28.
Dong, X., Zhou, H. & Dong, L. (1999) A new species and a new Chinese record of the subgenus Bothaella of genus Aedes (Diptera: Culicidae) [in Chinese]. Entomotaxonomia, 21, 133–137.
Drummond, A.J. & Rambaut, A. (2007) BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evolutionary Biology, 7, 214.
Edgar, R.C. (2004) MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32, 1792–1797.
Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–297.
Galliard, H. & Ngu, D.-V. (1947) Culicinés du Tonkin I. Liste des espèces et leur répartition. Annales de Parasitologie Humaine et Comparée, 22, 75–80.
Huelsenbeck, J.P. & Ronquist, F. (2001) MRBAYES: Bayesian inference of phylogeny. Bioinformatics, 17, 754–755.
Jermiin, L.S. & Crozier, R.H. (1994) The cytochrome b region in the mitochondrial DNA of the ant Tetraponera rufoniger: sequence divergence in Hymenoptera may be associated with nucleotide content. Journal of Molecular Evolution, 38, 282–294.
Liu, H. & Bechenback, A.T. (1992) Evolution of the mitochondrial cytochrome oxidase II gene among ten orders of insects. Molecular Phylogenetics and Evolution, 1, 41–52.
Reinert, J.F. (1973) Contributions to the mosquito fauna of Southeast Asia. –XIX. Bothaella, a new subgenus of Aedes Meigen. Contributions of the American Entomological Institute, 10(3), 1–51.
Reinert, J.F., Harbach, R.E. & Huong, V.D. (2006) Aedes (Finlaya) alongi Galliard and Ngu (Diptera: Culicidae: Aedini): description of the female, redescription of the fourth-instar larva, and reassignment to genus Bothaella Reinert. Zootaxa, 1135, 49–55.