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Issue: Vol. 2767 No. 1: 17 Feb. 2011
Type: Articles
Published: 2011-02-17
DOI: 10.11646/zootaxa.2767.1.1
Page range: 1–13
Abstract views: 58
PDF downloaded: 21

Steinernema pui sp. n. (Rhabditida, Steinernematidae), a new entomopathogenic nematode from Yunnan, China

State Key Lab of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People’s Republic of China
State Key Lab of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People’s Republic of China
Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd road, Guangzhou 510080, China
Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 74 Zhongshan 2nd road, Guangzhou 510080, China
State Key Lab of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, People’s Republic of China
Nematoda 28S rDNA sequence entomopathogenic nematode identification rDNA ITS sequence Steinernema pui sp. n. taxonomy

Abstract

A new species of entomopathogenic nematode, herein described as Steinernema pui sp. n. was recovered from a soil sample collected from Xiao-jie town, Jing-hong city, Xi-shuang-ban-na district in Yunnan province, the People’s Republic of China in December 2002. Both morphological and molecular evidence show congruently that S. pui sp. n. belongs to the S. glaseri group. It can be separated from all described Steinernema species by a combination of morphological and morphometrical characters of adult and juvenile stages, including spicule and gubernaculum shape of the first generation males (spicule bearing an aperture on the tip and an irregular-shaped concave on ventral side of the lamina close to the tip; gubernaculum with a short needle-shaped cuneus); the tail and vulva shape of the first generation females (tail conoid and pointed with a mucron; vulva with a short double flapped epiptygma) and the body and tail length, distance from anterior end to excretory pore and to the base of pharynx of infective juveniles. The new species can also be distinguished from other Steinernema species by DNA sequences of either a partial 28S rDNA or the internal transcribed spacer (ITS) regions of rDNA, and from the closely related species S. longicaudum and S. guangdongense by cross-breeding tests.

References

  1. Adams, B.J & Nguyen, K.B. (2002) Taxonomy and Systematics. In: Gaugler, R. (Ed.), Entomopathogenic Nematology, CABI Publishing, UK, pp. 1–33.

    Bedding, R.A. (1998) Future possibilities for using entomopathogenic nematodes. Japanese Journal of Nematology, 28, 46–60.

    Campbell, J.F., Lewis, E.F., Stock, S.P., Nadler, S. & Kaya, H.K. (2003) Evolution of host search strategies in entomopathogenic nematodes. Journal of Nematology, 35(2), 142–145.

    Chen, S.L., Li, X.H., Yan, A., Spiridonov, S.E. & Moens, M. (2006) A new entomopathogenic nematode, Steinernema hebeiense sp. n. (Rhabditida: Steinernematidae), from North China. Nematology, 8, 563–574.

    Courtney, W.D., Polley, D. & Miller, V.I. (1955) TAF an improved fixative in nematode technique. Plant Disease Reporter, 39, 570–571.

    Cutler, G.C. & Stock, S.P. (2003) Steinernema websteri sp. n. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from China. Nematologia Mediterranea, 31(2), 215–224.

    Hominick, W.M. (2002) Biogeography. In: Gaugler, R. (Ed.), Entomopathogenic Nematology, CABI Publishing, UK, pp. 167–211.

    Jian, H., Reid, A.P. & Hunt, D.J. (1997) Steinernema ceratophorum n. sp. (Nematoda: Steinernematidae) a new entomopathogenic from northeast China. Systematic Parasitology, 37, 115–125.

    Liu, J. (1990) The description of new species and taxonomic study of the genus: Steinernema Travassos & Heterorhabditis Poinar (entomopathogenic nematodes). PhD Thesis, School of Plant Protection, Agricultural University of Southern China, Guangzhou, China, 127pp.

    Liu, J. (1994) A new species of Heterorhabditis (Nematoda: Heterorhabditidae) from China. Acta Zootaxonomica Sinica, 19, 268–272.

    Mráček, Z. (1980) The use of Galleria traps for obtaining nematode parasites of insects in Czechoslovakia (Lepidoptera: Nematoda, Steinernematidae). Acta Entomologica Bohemoslovaca, 77(6), 378–382.

    Mráček, Z., Nguyen, K.B., Tailliez, P., Boemare, N. & Chen, S. (2006) Steinernema sichuanense n. sp. (Rhabditida, Steinernematidae), a new species of entomopathogenic nematode from the province of Sichuan, east Tibetan Mts., China. Journal of Invertebrate Pathology, 102(1), 69–78.

    Mráček, Z., Qi-Zhi, L. & Nguyen, K.B. (2009) Steinernema xueshanense n. sp. (Rhabditida, Steinernematidae), a new species of entomopathogenic nematode from the province of Yunnan, southeast Tibetan Mts., China. Journal of Invertebrate Pathology, 93(3), 157–69.

    Nguyen, K.B., Malan A.P. & Gozel, U. (2007) Steinernema khoisanae n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from South Africa. Nematology, 8(2), 157–175.

    Nguyen, K.B., Maruniak, J. & Adams, B.J. (2001) The diagnostic and phylogenetic utility of the rDNA internal transcribed spacer sequences of Steinernema. Journal of Nematology, 33, 73–82.

    Nguyen, K.B., Qiu, L., Zhou, Y. & Pang, Y. (2006) Steinernema leizhouense sp. n. (Nematoda: Steinernematidae), a new entomopathogenic nematode from southern China. Russian Journal of Nematology, 14(2), 101–118.

    Nguyen, K.B. & Smart, Jr. G.C. (1995) Scanning electron microscope studies of Steinernema glaseri (Nematoda: Steinernematidae). Nematologica, 41, 183–190.

    Nguyen, K.B. & Smart, Jr. G.C. (1997) Scanning electron microscope studies of spicules and gubernacula of Steinernema spp. (Nemata: Steinernematidae). Nematologica, 43, 465–480.

    Poinar, G.O. (1967) Description and taxonomic position of the DD-136 nematode (Steinernematidae, Rhabditoidea) and its relationship to Neoaplectana carpocapsae Weiser. Proceedings of Helminthological society of Washington, 34, 199–209.

    Qiu, L., Fang, Y., Zhou, Y., Pang, Y. & Nguyen, K.B. (2004) Steinernema guangdongense sp. n. (Nematoda: Steinernematidae), a new entomopathogenic nematode from southern China with a note on S. serratum (nomen nudum). Zootaxa, 704, 1–20.

    Qiu, L., Yan, X., Zhou Y., Pang, Y. & Nguyen, K.B. (2005a) Steinernema aciari sp. n. (Nematoda: Steinernematidae), a new entomopathogenic nematode from Guangdong, China. Journal of Invertebrate Pathology, 88(1), 58–69.

    Qiu, L., Hu, X., Zhou, Y., Nguyen, K.B. & Pang, Y. (2005b) Steinernema beddingi n. sp. (Nematoda: Steinernematidae), an entomopathogenic nematode from Yunnan, China. Nematology, 7(5), 755–767.

    Qiu, L., Hu X., Zhou, Y., Nguyen, K.B. & Pang, Y. (2005c) Steinernema akhursti n. sp. (Nematoda: Steinernematidae) from Yunnan, China. Journal of Invertebrate Pathology, 90(3), 151–160.

    Saitou, N. & Nei, M. (1987) The Neighbor-joining Method: A New Method for Reconstructing Phylogenetic Trees. Molecular and Biological Evolution, 4(4), 406–425.

    Seinhorst, J.W. (1959) A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica, 4, 67–69.

    Shen, C.P. & Wang, G.H. (1991) Description and studies of an entomopathogenic nematode: Steinernema longicaudum sp. nov. Proceeding of the first national academy symposium, Chinese Science and Technology Press, 220–231.

    Stock, S.P. & Koppenhöfer, A.M. (2003) Steinernema scarabaei n. sp. (Rhabditida: Steinernematidae), a natural pathogen of scarab beetle larvae (Coleoptera: Scarabaeidae) from New Jersey, USA. Nematology, 5(2), 191–204.

    Stock, S.P., Campbell, J.F. & Nadler, S.A. (2001) Phylogeny of Steinernema Travassos, 1927 (Cephalobina: Steinernematidae) inferred from ribosomal DNA sequences and morphological characters. Journal of Parasitology, 87, 877–889.

    Tamura, K., Dudley, J., Nei, M. & Kumar, S. (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24, 1596–1599.

    Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876–4882.

    Waturu, C.N., Hunt, D.J. & Reid, A.P. (1997) Steinernema karii sp. n. (Nematoda: Steinernematidae), a new entomopathogenic nematode from Kenya. International Journal of Nematology, 7, 68–75.

    White, G.F. (1927) A method for obtaining infective nematodes larvae from culture. Science, 66, 302–303.

    Wilbur, W.J. & Lipman, D.J. (1983) Rapid similarity searches of nucleic acid and protein data banks. Proceedings of National Academics of Sciences USA, 80, 726–730.

    Xu, Z., Wang, G. & Li, X. (1991) A new species of the genus Steinernema (Rhabditida: Steinernematidae). Zoological Research, 12, 17–20.