Abstract
The third species of the genus Prochetostoma, developing in the fruits of Ilex integra Thunberg, has been reported six times in Japan since 1985 but has not been described. We here describe adults and immatures of this species as P. expandens sp. n. Based on the adult morphological characters, P. expandens is suggested to be the sister species of P. bhutanicum Han. Two possible morphological synapomorphies of these two species are proposed. We also investigated a large number of larvae of P. expandens collected from fruits of I. integra. Detailed information about the life history is presented: oviposition takes place under the skin of immature fruits of I. integra in spring; larvae consume fruit tissue in summer and autumn; single fully matured larva escapes from the fruit and drops down to the ground for pupation; and adults emerge in the subsequent spring.References
Cumming, J.M., Sinclair, B.J. & Wood, D.M. (1995) Homology and phylogenetic implications of male genitalia in Diptera –Eremoneura. Entomologica Scandinavica, 26, 121–151.
Galle, F.C. (1997) Hollies. The genus Ilex. Timber Press Inc, Portland, 573 pp.
Han, H.-Y. (1992) Classification of the tribe Trypetini (Diptera: Tephritidae: Trypetinae). Ph.D. dissertation, the Pennsylvania State University, University Park, 274 pp.
Han, H.-Y. (1998) A list of the reported host plants of the tribe Trypetini (Diptera: Tephritidae). Korean Journal of Entomology, 28, 355–368.
Han, H.-Y. (1999) Phylogeny and behavior of flies in the tribe Trypetini (Trypetinae). In: Aluja, M. & Norrbom, A. (Eds), Fruit Flies (Tephritidae): Phylogeny and Evolution of Behavior. CRC Press, Boca Raton, pp. 253–297.
Han, H.-Y. (2000) Molecular phylogenetic study of the tribe Trypetini (Diptera: Tephritidae), using mitochondrial 16S ribosomal DNA sequences. Biochemical Systematic Ecology, 28(6), 75–87.
Han, H.-Y. (2006) Prochetostoma, a new genus, proposed as a basal group of the subtribe Chetostomatina (Diptera: Tephritidae). In: Freidberg, A. (Ed.), Biotaxonomy of Tephritoidea. Israel Journal of Entomology, 35–36, 147–162.
Han, H.-Y. & McPheron, B.A. (1997) Molecular phylogenetic study of Tephritidae (Insecta: Diptera) using partial sequences of the mitochondrial 16S ribosomal DNA. Molecular Phylogenetics and Evolution, 7, 17–32.
Han, H.-Y. & McPheron, B.A. (1999) Nucleotide sequence data as a tool to test phylogenetic relationships among higher
groups of Tephritidae: a case study using mitochondrial ribosomal DNA. In: Aluja, M. & Norrbom, A. (Eds), Fruit Flies
(Tephritidae): Phylogeny and Evolution of Behavior. CRC Press, Boca Raton, pp. 115–132.
Han, H.-Y. & Ro, K.-E. (2002) Molecular systematics of Tephritidae (Insecta: Diptera): testing phylogenetic position of Korean Acidiella spp. (Trypetini) using mitochondrial 16S rDNA sequences. Korean Journal of Biological Science, 6, 13–18.
Han, H.-Y. & Ro, K.-E. (2009) Molecular phylogeny of family Tephritidae (Insecta: Diptera): New insight from combined
analysis of the mitochondrial 12S, 16S, and COII genes. Molecules and Cells, 27, 55–66.
Horikawa, Y. (1972) Atlas of the Japanese Flora. Gakken Co., Ltd, Tokyo, 500 pp.
Ichita, T. (1996) The family Tephritidae in the Kanagawa Prefecture (II). The specimens collected by Mr. Ichiro Waki in 1995. Kanagawa Chuho, 115, 15–18. (In Japanese)
Iwase, K., Otsubo, H., Kubo, K., Shiho, T. & Watari, H. (2000) The insects in the Mount Enkai Area. Kanagawa Chuho, 130, 1–458. (In Japanese)
Japanese Society of Applied Entomology and Zoology (2006) Major insect and other pests of Economic Plants in Japan.
Revised version. Kokusai Bunken Insatsu, Tokyo, 387 pp. (In Japanese)
McAlpine, J.F. (1981) Morphology and terminology – adults. In: McAlpine, J. F. et al. (Eds), Manual of Nearctic Diptera, 1. Research Branch, Agriculture Canada Monograph, 27, 9–63.
Sasaki, F., Fuke, S. & Masuda, H. (1985) Notes on some fruit flies collected by lure trapping and host plant survey. Research Bulletin of Plant Protection, Japan, 21, 11–16. (In Japanese, with English title and abstract)
Stuckenberg, B.R. (1999) Antennal evolution in the Brachycera (Diptera), with a reassessment of terminology relating to the flagellum. Studia dipterologica, 6, 33–48.
Sueyoshi, M. (1999) Immature stages of three Oriental species of the genus Rhabdochaeta de Meijere (Diptera: Tephritidae), with brief biological notes. Entomological Science, 2, 217–230.
Sueyoshi, M. (2000) Tephritid flies of the Imperial Palace, Japan. Memoir of the National Science Museum, Tokyo, 36, 437–443. (In Japanese, with English title and summary)
Sueyoshi, M. (2005a) Tephritidae. In Gakken Co., Ltd. (Ed), Insect Larvae of Japan, Gakken, Tokyo, pp. 305–307. (In Japanese)
Sueyoshi, M. (2005b) Tephritid flies of the Akasaka Imperial Gardens and the Tokiwamatsu Imperial Villa, Tokyo, Japan.
Memoir of the National Science Museum, Tokyo, 39, 333–336. (In Japanese, with English title and summary)
Sueyoshi, M. (2006) Comparative morphology of the male terminalia of Tephritidae and other Cyclorrhapha In: Freidberg, A. (Ed.), Biotaxonomy of Tephritoidea. Israel Journal of Entomology, 35–36, 477–496.
White, I.M., Headrick, D.H., Norrbom, A.L. & Carroll, L.E. (1999) Glossary. In: Aluja, M. & Norrbom, A. (Eds), Fruit Flies (Tephritidae): Phylogeny and Evolution of Behavior. CRC Press, Boca Raton, pp. 881–924.
Yamazaki, T. (1989) Aquifoliaceae. In: Satake et al. (Eds) Wild Flowers of Japan. Woody plants. Heibonsha, Ltd., Tokyo, pp.26–32. (In Japanese)
Yukawa, J. & Masuda, H. (1997) Insect and mites galls of Japan in colors. Zenkoku Norin Kyoiku Kyokai, Tokyo, 826 pp. (In Japanese with English title and captions for plates)