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Type: Articles
Published: 2011-05-10
Page range: 35–50
Abstract views: 63
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Morphological and molecular delineation of a new species in the Ceratina dupla species-group (Hymenoptera: Apidae: Xylocopinae) of eastern North America

Department of Biological Sciences, Brock University, 500 Glenridge Avenue, St. Catharines, Ontario, Canada L2S 3A1
Department of Biology, York University, 4700 Keele Street, Toronto, Ontario, Canada M3J 1P3
Hymenoptera small carpenter bee DNA barcodes cryptic species Ceratina (Zadontomerus) Ceratina floridana Ceratina mikmaqi

Abstract

DNA barcoding is used to verify characters to morphologically differentiate genetically distinct species of eastern North American small carpenter bees, Ceratina. Here we reveal that the common eastern North American species, Ceratina dupla s. l., is actually three separate species based on fixed differences in DNA barcode sequences and morphological characters. This study adds a new species, C. mikmaqi Rehan & Sheffield, to the Ceratina dupla species-group of eastern North America, and raises another form, C. floridana formerly C. dupla floridana, to full species. Temporal niche partitioning between C. dupla and C. mikmaqi and geographic isolation of C. floridana further support the division of the C. dupla s. l. group into three species. A diagnosis and description of the new species are provided, as is a key for eastern North American species of Ceratina.

References

  1. Avise, J.C. & Walker, D. (1999) Species realities and numbers in sexual vertebrates: perspectives from an asexually transmitted genome. Proceedings of the National Academy of Science USA, 96, 992–995.

    Clinebell, R.R. (2002) Foraging ecology in selected prairie wildflowers (Echinacea, Liatris, Monarda, and Veronicastrum) in Missouri prairie remnants and restorations. Proceedings of the 18th North America Prairie Conference. Truman State University, Kirksville, Missouri, pp. 194–212.

    Cronin, A.L. (2004) A molecular phylogeny and social behaviour of Japanese Ceratina (Hymenoptera, Apidae, Xylocopinae). Insect Systematics and Evolution, 35, 137–146.

    Daly, H.V. (1966) Biological studies on Ceratina dallatorreana, an alien bee in California which reproduces by parthenogenesis (Hymenoptera: Apoidea). Annals of the Entomological Society of America, 59, 1138–1154.

    Daly, H.V. (1973) Bees of the Genus Ceratina in America North of Mexico. University of California Press, Berkley, California, 114 pp.

    Danforth, B.N., Mitchell, P.L. & Packer, L. (1998) Mitochondrial DNA differentiation between two cryptic Halictus (Hymenoptera: Halictidae) species. Annals of the Entomological Society of America, 91, 387–391.

    Deyrup, M., Edirisinghe, J. & Norden, B. (2002) The diversity and floral hosts of bees at the Archbold Biological Station, Florida (Hymenoptera: Apoidea). Insecta Mundi, 16, 87–120.

    Dunn, M., Mitchell, P.L. & Packer, L. (1998) Phenology and social biology of two sibling species of Halictus in an area of sympatry. Canadian Journal of Zoology, 76, 2207–2213.

    Excoffier, L., Laval, G. & Schneider, S. (2005) Arlequin ver. 3.0: An integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online, 1, 47–50.

    Eldridge, N. & Cracraft, J. (1980) Phylogenetic Patterns and the Evolutionary Process. Columbia University Press, New York.

    Gibbs, J. (2009) An integrative taxonomic approach reveals new (and old) species in the Lasioglossum (Dialictus) tegulare (Robertson) species group (Hymenoptera, Halictidae). Zootaxa, 2032, 1–38.

    Gibbs, J. (2010) Revision of the metallic species of Lasioglossum (Dialictus) of Canada (Hymenoptera, Halictidae, Halictini). Zootaxa, 2591, 1–382.

    Ginsberg, H.S. (1984) Foraging behaviour of the bees Halictus ligatus (Hymenoptera: Halictidae) and Ceratina calcarata (Hymenoptera: Anthophoridae) foraging speed on early-summer composite flowers. New York Entomological Society, 92, 162–168.

    Grissell, E.E. (1976) The carpenter bees of Florida (Hymenoptera: Apidae: Xylocopinae). Florida Department of Agriculture and Consumer Services, Division of Plant Industry, Entomology Circular, No. 167.

    Grothaus, R.H. (1962) The biology of the species of Ceratina (Hymenoptera, Xylocopidae) in Indiana. Unpublished Master’s thesis, Purdue University, Lafayette, Indiana, 201 pp.

    Hajibabaei, M., deWaard, J.R., Ivanova, N.V., Ratnasingham, S., Dooh, R.T., Kirk, S.L., Mackie, P. & Hebert, P.D.N. (2005) Critical factors for assembling a high volume of DNA barcodes. Philosophical Transactions of the Royal Society of London (Series B), 360, 1959–1967.

    Hall, T.A. (1999) Bioedit: a user friendly biological sequence alignment editor and alignment program for Windows 95/98 NT. Nucleic Acid Symposium Series, 41, 95–98.

    Hebert, P.D.N., Cywinska, A., Ball, S.L. & deWaard, J.R. (2003a) Biological identifications through DNA barcodes. Proceedings of the Royal Society of London Series B—Biological Sciences, 270, 313–321.

    Hebert, P.D.N., Penton, E.H., Burns, J.M., Janzen, D.H. & Hallwachs, W. (2004a) Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences of the United States of America, 101, 14812–14817.

    Hebert, P.D.N., Ratnasingham, S. & deWaard, J.R. (2003b) Barcoding animal life: cytochrome c oxidase subunit 1divergences among closely related species. Proceedings of the Royal Society of London Series B—Biological Science, 270, S96–S99.

    Hebert, P.D.N., Stoeckle, M.Y., Zemlak, T.S. & Francis, C.M. (2004b) Identification of birds through DNA barcodes. PLoS Biology, 2, e312.

    Ivanova, N., deWaard, J. & Hebert, P. (2006) An inexpensive, automation-friendly protocol for recovering high-quality DNA. Molecular Ecology Notes, 6, 998–1002.

    Johnson, M.D. (1988) The relationship of provision weight to adult weight and sex ratio in the solitary bee, Ceratina calcarata. Ecological Entomology, 13, 165–170.

    Kimura, M. (1980) A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111–120.

    Leuck, D.B. & Hammons, R.O. (1969) Occurrence of atypical flowers and some associated bees (Apoidea) in the peanut, Arachis hypogaea L. Agronomy Journal, 61, 958–960.

    Mayr, E. (1942) Systematics and the Origin of Species. Harvard University Press, New York, 372 pp.

    Michener, C.D. (1954) The bees of Panama. Bulletin of the American Museum of Natural History, 104, 1–176.

    Michener, C.D. (2007) The Bees of the World, second ed. John Hopkins University Press, Baltimore, xvi+[i]+953 pp.

    Mitchell, R.S. (2002) New York Flora Association Newsletter—New York State Museum Associates, New York State Museum, Vol. 13, No. 4 (September).

    Mitchell, T. (1962) Bees of the Eastern United States, Volume 2. North Carolina Agricultural Experiment Station Technical Bulletin Number 152. North Carolina, USA. 557 pp.

    Moore, W.S. (1995) Inferring phylogenies from mtDNA variation: Mitochondrial-gene trees versus nuclear-gene trees. Evolution, 49, 718–726.

    Moure, J.S. (2007) Xylocopini Latreille, 1802. In: Moure, J. S., Urban, D. & Melo, G. A. R. (Eds), Catalogue of Bees (Hymenoptera, Apoidea) in the Neotropical Region. Sociedade Brasileira de Entomologia, Curitiba, pp. 637–673.

    Nelson, G. & Platnick, N. (1981) Systematics and Biogeography: Cladistics and Vicariance. Columbia University Press, New York. 567 pp.

    Packer, L. (1999) The distribution of Halictus ligatus Say and H. poeyi Lep. (Hymenoptera: Halictidae) in North America. In: Byers, C. W., R. H. Hagen, and R. W. Brooks (Eds.), Entomological Contributions in Memory of Byron A. Alexander. University of Kansas Natural History Museum Special Publication 24, pp. 81–84.

    Packer, L., Gibbs, J., Sheffield, C. & Hanner, R. (2009) DNA barcoding and the mediocrity of morphology. Molecular Ecology Resources. 9 (Suppl. 1), 42–50.

    Reed, C.C. (1995) Species richness of insects on prairie flowers in southeastern Minnesota. In: Hartnett, D. C. (Ed.) Fourteenth North American Prairie Conference: Prairie Biodiversity. Kansas State University, Manhattan, Kansas, pp. 103–115.

    Rehan, S.M. & Richards, M.H. (2008) Morphological and DNA sequence delineation of two problematic species of Ceratina (Hymenoptera: Apidae) from Eastern Canada. Journal of the Entomological Society of Ontario, 139, 59–67.

    Russell, K.N., Ikerd, H. & Droege, S. (2005) The potential conservation value of unmowed powerline strips for native bees. Biological Conservation, 124, 133–148.

    Sakagami, S.F. & Maeta, Y. (1977) Some presumably presocial habits of Japanese Ceratina bees, with notes on various social types in Hymenoptera. Insectes Sociaux, 24, 319–343.

    Sheffield, C.S., Kevan, P.G., Smith, R.F., Rigby, S.M. & Rogers, R.E.L. (2003) Bee species of Nova Scotia, Canada, with new records and notes on bionomics and floral relations (Hymenoptera: Apoidea). Journal of the Kansas Entomological Society, 76, 357–384.

    Sheffield, C.S., Hebert, P.N., Kevan, P.G. & Packer, L. (2009) DNA barcoding a regional bee (Hymenoptera: Apoidea) fauna and its potential for ecological studies.Molecular Ecology Resources, 9, 196–207.

    Sheffield, C.S. & Westby, S.M. (2007) The male of Megachile nivalis Friese, with an updated key to members of the subgenus Megachile s. str. (Hymenoptera: Megachilidae) in North America. Journal of Hymenoptera Research, 16, 178–191.

    Shiokawa, M. (1963) Redescriptions of Ceratina flavipes Smith and C. japonica Cockerell (Hymenoptera, Apidae). Kontyu, 31, 276–280.

    Swofford, D.L. (2003) PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version b4.10. Sinauer Associates, Sunderland, Massachusetts.

    Terzo, M. (2000) Classification phylogénétique des Cératines du monde et monographie des espèces de la région ouest-paléarctique et de l'Asie Centrale (Hymenoptera, Apoidea, Xylocopinae: Ceratina Latreille). Ph.D. dissertation, Universite de Mons-Hainaut, 293 pp.

    Thompson, J.D., Higgins, D.G. & Gibons, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acid Research, 22, 4673–4680.

    Thompson, J.D., Gibson, T.J., Plewniak, F, Jeanmougin, F. & Higgins, D.G. (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25, 4876–4882.

    Tuell, J.K., Ascher, J.S. & Isaacs, R. (2009) Wild Bees (Hymenoptera: Apoidea: Anthophila) of the Michigan Highbush Blueberry Agroecosystem. Annals of the Entomological Society of America, 102, 275–287.

    Tuell, J.K., Fiedler, A.K., Landis, D. & Isaacs, R. (2008) Visitation by wild and managed bees (Hymenoptera: Apoidea) to eastern U.S. native plants for use in conservation programs. Environmental Entomology, 37, 707–718.

    Vickruck, J.L., Rehan, S.M., Sheffield, C.S. & Richards, M.H. (in press) Nesting Biology and barcode analysis of two cryptic species within Ceratina dupla, with comparisons to C. calcarata (Hymenoptera: Apidae: Xylocopinae). Canadian Entomologist.

    Winfree, R., Williams, N.M., Dushoff, J. & Kremen, C. (2007) Native bees provide insurance against ongoing honey bee losses. Ecology Letters, 10, 1105–1113.

    Yasumatsu, K. & Hirashima, Y. (1969) Synopsis of the small carpenter bee genus Ceratina of Japan. Kontyu, 37, 61–70.