Abstract
We describe a new small sized (SVL in females ≤ 25.7 mm; in males, ≤ 20.1 mm) Pristimantis, associated with bamboodominated forests in the Reserva Mazar, Andes of Southeastern Ecuador, at elevations between 2876–2989 m. This species is assigned to the P. orestes group, from whose members it differs by the absence of tubercles on heel and outer edge of tarsus, lacking a discoidal fold, presence of slightly expanded finger and toe pads, and bright yellow blotches on groin, axilla and anterior surfaces of thigh. The advertisement call of the new species consists of a series of short, indistinctly pulsed notes, and has a dominant frequency of 2.50–2.56 kHz. A molecular phylogeny based on a fragment of the mitochondrial gene 12S shows that the new species is sister to a clade formed by Pristimantis simonbolivari and an undescribed Pristimantis.References
AmphibiaWeb. (2011) Information on amphibian biology and conservation. [web application]. 2011. Berkeley, California.: AmphibiaWeb. Available:Available from http://amphibiaweb.org/. (Accessed: Jan 2011).
Boulenger, G.A. (1900) Descriptions of new batrachians and reptiles collected by Mr. P.O. Simons in Peru. Annals and Magazines of Natural History, Series 7, 6, 181–186.
Charif, R.A., Clark, C.W. & Fristrup, K.M. (2004) Raven 1.2 user’s manual. Cornell Laboratory of Ornithology, Ithaca, New York, USA.
Duellman, W.E. & Lehr, E. (2009) Terrestrial breeding frogs (Strabomantidae) in Peru. Natur und Tier – Verlag, Naturwissenschaft, Münster, 384 pp.
Duellman, W.E. & Pramuk, J.B. (1999) Frogs of the genus Eleutherodactylus (Anura: Leptodactylidae) in the Andes of northern Peru. Scientific Papers, Natural History Museum University of Kansas, 13, 1–78.
Duellman, W. E. & Trueb, L. (1994) Biology of Amphibians. The Johns Hopkins University Press, Baltimore and London, 670 pp.
Duellman, W.E., Lehr, E. & Venegas, P.J. (2006) Two new species of Eleutherodactylus (Anura: Leptodactylidae) from the Andes of northern Peru. Zootaxa, 1285, 51–64.
FCT. (2008) Nudo del Azuay Conservation Initiative: An Introduction to the Dudas Watershed. Fundación Cordillera Tropical, Cuenca, Ecuador.
Frost, D.R. (2009) Amphibian Species of the World: an Online Reference. Version 5.3. Available from: http://research.amnh.org/herpetology/amphibia/ (accessed 22 March, 2010).
González-Voyer, A., Padial, J.M., Castroviejo-Fisher, S., De la Riva, I., Vilà. (2011) Correlates of species richness in the largest Neotropical amphibian radiation. Journal of Evolutionary Biology, doi: 10.1111/j.1420-9101.2011.02243.x
Hedges, S.B., Duellman, W.E. & Heinicke, P. (2008) New world direct-developing frogs (Anura: Terrarana): molecular phylogeny, classification, biogeography, and conservation. Zootaxa, 1737, 1–182.
Heinicke, M.P., Duellman, W.E. & Hedges, S.B. (2007) Major Caribbean and Central American frog faunas originated by ancient oceanic dispersal. Proceedings of the National Academy of Sciences of the United States of America, 104, 10092–10097.
Lehr, E. (2007) New Eleutherodactyline frogs (Leptodactylidae: Pristimantis, Phrynopus) from Peru. Bulletin of the Museum of Comparative Zoology, 159, 145–178.
Lehr, E. & Coloma, L.A. (2008) A minute new Ecuadorian Andean frog (Anura: Strabomantidae, Pristimantis). Herpetologica, 64, 354–367.
Lehr, E. & Duellman, W.E. (2007) A diminutive new species of Pristimantis (Amphibia: Anura: Leptodactylidae) from northern Peru. Salamandra, 43, 165–171.
Lehr, E. & Köhler, G. (2007) A new species of the Pristimantis orestes Group (Anura: Leptodactylidae) from the Cordillera Occidental in northern Peru. Zootaxa, 1621, 45–54.
Lynch, J.D. (1979) Leptodactylid frogs of the genus Eleutherodactylus from the Andes of southern Ecuador. Miscellaneous Publications, Museum of Natural History, University of Kansas, 66, 1–62.
Lynch, J.D., & Duellman, W.E. (1980) The Eleutherodactylus of the Amazonian slopes of the Ecuadorian Andes (Anura: Leptodactylidae). Miscellaneous Publications, Museum of Natural History, University of Kansas, 69, 1–86.
Lynch, J.D. & Duellman, W.E. (1997) Frogs of the genus Eleutherodactylus (Leptodactylidae) in western Ecuador: systematics, ecology, and biogeography. Miscellaneous Publications of the Natural History Museum, University of Kansas, 23, 1–236.
Maddison, D.R. & Maddison, W.P. (2005) MacClade. Sinauer Associates, Sunderland, Massachusetts.
Valencia, R., Cerón, C., Palacios, W. & Sierra, R. (1999) Las formaciones naturales de la Sierra del Ecuador. In: Sierra, R. (Ed.), Propuesta Preliminar preliminar de un sistema de Clasificacin clasificación de Vegetación vegetación para el Ecuador Continentalcontinental. Proyecto INEFAN/GEF– BIRF and Ecociencia, Quito, Ecuador, pp. 79–108.
Vences, M. & Wake, D.B. (2007) Speciation, species boundaries and phylogeography of amphibians. In: Heatwole, H. H. & M. Tyler (eds.), Amphibian Biology, Vol. 6, Systematics, Surrey Beatty & Sons, Chipping Norton, Australia, pp. 2613–2669.
Wiens, J.J. (2004) Speciation and ecology revisited: phylogenetic niche conservatism and the origin of species. Evolution, 58, 193–197.
Wiens, J.J., & Coloma, L.A. (1992) A new species of the Eleutherodactylus myersi (Anura: Leptodactylidae) assembly from Ecuador. Journal of Herpetology, 26, 196–207.
Wiens, J.J., Fetzner, J.W., Parkinson, C.L. & Reeder, T.W. (2005) Hylid frog phylogeny and sampling strategies for speciose clades. Systematic Biology, 54, 719–748.
Zwickl, D.J. (2006) Genetic algorithm approaches for the phylogenetic analysis of large biological sequence datasets under the maximum likelihood criterion. Ph.D. dissertation, The University of Texas at Austin.