Abstract
We generated a multi-locus phylogeny to test monophyly and distributional limits in Australian toadlets of the genus Uperoleia from the western arid zone of Australia. The molecular data were used in combination with a detailed assessment of morphological variation and some data on call structure to complete a taxonomic revision of the species that occur in this region. Our work reveals the existence of not two but five species in the region. Uperoleia russelli is restricted to the Carnarvon and Gascoyne Regions south of the Pilbara. Uperoleia micromeles is distributed from the Tanami Desert through the Great Sandy Desert and along the northern edge of the Pilbara. Uperoleia talpa was previously believed to be a Fitzroyland region endemic but it is further distributed along Dampierland and into the Roebourne Plain. Uperoleia glandulosa is a larger species than previously described as well as a greater habitat generalist, inhabiting the rocky Pilbara region and the sandy region around Port Hedland. We also describe a new species, U. saxatilis sp. nov., endemic to the Pilbara craton.References
Adalsteinsson, S.A., Branch, W.R., Trape, S., Vitt, L.J. & Hedges, S.B. (2009) Molecular phylogeny, classification, and biogeography of snakes of the Family Leptotyphlopidae (Reptilia, Squamata). Zootaxa, 2244, 1–50.
Barker, J., Grigg, G.C. & Tyler, M.J. (1995) A field guide to Australian frogs. Surry Beatty & Sons: Chipping Norton, p. 339.
Beard, J.S., & Webb, M.J. (1974) Vegetation of Western Australia - Great Sandy Desert, 1:1,000,000 vegetation series: explanatory notes to sheet 2. Nedlands, W.A.: University of Western Australia Press.
Beard, J.S. (1975) Vegetation of Western Australia - Pilbara, 1:1,000,000 vegetation series: explanatory notes to sheet 5: the vegetation of the Pilbara area. Nedlands, W.A.: University of Western Australia Press.
Beard, J.S. (1979) Vegetation of Western Australia - Kimberley, 1:1,000,000 vegetation series: explanatory notes to sheet 1, the vegetation of the Kimberley area. Nedlands, W.A.: University of Western Australia Press.
Blake, A.J.D. (1973) Taxonomy and relationships of Myobatrachine Frogs (Leptodactylidae): A numerical approach. Australian Journal of Zoology, 21, 119–149.
Chiari, Y., Vences, M., Vieties, D.R., Rabemananjara, S.F., Bora, P., Ravoahangimalala, O.R. & Meyer, A. (2004) New evidence for parallel evolution of color patterns in Malagasy poison frogs (Mantella). Molecular Ecology, 13, 3763–3774.
Cogger, H.G. (2000) Reptiles and Amphibians of Australia. 6th Ed. Reed New Holland, Sydney, p. 808.
Commonwealth of Australia (2005) Interim Biogeographic Regions of Australia version 6.1. Department of Environment, Water, Heritage and the Arts. Available from: www.environment.gov.au/parks/nrs/science/ibra.html (accessed Sept. 8 2010).
Crawford, A.J. & Smith, E.N. (2005) Cenozoic biogeography and evolution in direct-developing frogs of Central America (Leptodactylidae: Eleutherodactylus) as inferred from a phylogenetic analysis of nuclear and mitochondrial genes. Molecular Phylogenetics and Evolution, 35, 536–555.
Crisp, M.D., Laffan, S., Linder, H.P. & Monro, A. (2001) Endemism in Australian Flora. Journal of Biogeography, 28, 183–198.
Darst, C.R. & Cannatella, D.C. (2004) Novel relationships among hyloid frogs inferred from 12S and 16S mitochondrial DNA sequences. Molecular Phylogenetics and Evolution, 31, 462–475
Davies, M., Mahony, M. & Roberts, J.D. (1985) A new species of Uperoleia (Anura: Leptodactylidae) from the Pilbara region, Western Australia. Transactions of the Royal Society of South Australia, 109(3), 103–108.
Davies, M. & Martin, A.A. (1988) Redefinition of Uperoleia talpa Tyler, Davies & Martin, 1981 (Anura: Leptodactylidae: Myobatrachidae). Transactions of the Royal Society of South Australia, 112, 87–89.
Davies, M. & McDonald, K.R. (1998) Developmental biology of Uperoleia altissima Davies, Watson, McDonald, Trenerry & Werren, 1993 (Anura: Myobatrachidae). Transactions of the Royal Society of South Australia, 122(4), 167–172.
Davies, M., McDonald, K.R. & Corben, C. (1986) The genus Uperoleia Gray (Anura: Leptodactylidae) in Queensland, Australia. Proceedings of the Royal Society of Victoria, 98(4), 147–188.
Davies, M. & Watson, G.F. (1998) Developmental biology of Uperoleia talpa Tyler, Davies & Martin, 1981 (Anura: Myobatrachidae). Transactions of the Royal Society of South Australia, 122(4), 153–157.
Doughty, P., Pepper, M. & Keogh, J.S. (2010) Morphological and molecular assessment of the Diplodactylus savagei species complex in the Pilbara region, Western Australia, with a description of a new species. Zootaxa, 2393, 33–45.
Doughty, P. & Roberts, J.D. (2008) A new species of Uperoleia (Anura: Myobatrachidae) from the northwest Kimberley, Western Australia. Zootaxa, 1939, 10–18.
Drummond, A.J., Ho, S.Y.W., Phillips, M.J. & Rambaut, A. (2006) Relaxed Phylogenetics and Dating with Confidence. PLoS Biology, 4(5), 699–710.
Drummond, A.J. & Rambaut, A. (2007) BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evolutionary Biology, 7, 214.
Evans, B.J., Brown, R.M., McGuire, J.A., Supriatna, J., Andayani, N., Diesmos, A., Iskandar, D., Melnick, D.J. & Cannatella, D.C. (2003) Phylogenetics of Fanged Frogs: Testing Biogeographical Hypotheses at the Interface of the Asian and Australian Faunal Zones. Systematic Biology, 52(6), 794–819.
Frost, D.R., Grant, T., Faivovich, J., Bain, R.H., Haas, A., Haddad, C.F.B., De Sá, R. O., Channing, A., Wilkinson, M., Donnellan, S.C., Raxworthy, C.J., Campbell, J.A., Blotto, B.L., Moler, P., Drews, R.C., Nussbaum, R.A., Lynch, J.D., Green, D.M. & Wheeler, W.C. (2006) The Amphibian tree of life. Bulletin of the American Museum of Natural History, 297, 1–370.
Fujita, M.K., McGuire, J.A., Donnellan, S.C. & Moritz, C. (2010) Diversification and persistence at the arid-monsoonal interface: Australia-wide biogeography of the Bynoe’s Gecko (Heteronotia binoei; Gekkonidae). Evolution, 64, 2293–2010.
Gamble, T., Berendzen, P.B., Shaffer, H.B., Starkey, D.E. & Simons, A.M. (2008) Species limits and phylogeography of North America cricket frogs (Acris: Hylidae). Molecular Phylogenetics and Evolution, 48, 112–125.
Gartside, D.F., Littlejohn, M.J. & Watson, G.F. (1979) Structure and dynamics of a narrow hybrid zone between Geocrinia laevis and G. victoriana (Anura:Leptodactylidae) in South-Eastern Australia. Heredity, 43, 165–177.
Garda, A.A. & Cannatella, D.C. (2007) Phylogeny and biogeography of paradoxical frogs (Anura, Hylidae, Pseudae) inferred from 12S and 16S mitochondrial DNA. Molecular Phylogenetics and Evolution, 44, 104–114.
Guarnizo, C.E., Amezquita, A. & Bermingham, E. (2008) The relative roles of vicariance versus elevational gradients in the genetic differentiation of the high Andean tree frog, Dendropsophus labialis. Molecular Phylogenetics and Evolution, 50, 84–92.
Hickman, A.H. (1983) Geology of the Pilbara Block and its environ. Western Australia Geological Survey, Bulletin. 127.
Hoegg, S., Vences, M., Brinkmann, H. & Meyer, A. (2008) Phylogeny and comparative substitution rates of frogs inferred from sequences of three nuclear genes. Molecular Biology and Evolution, 21, 1118–1200.
Ladiges, P., Parra-O, C., Gibbs, A., Udovicic, F., Nelson, G. & Bayly, M. (2010) Historical biogeographical patterns in continental Australia: congruence among areas of endemism of two major clades of eucalypts. Cladistics, 26, 1–13.
Macey, J.R., Schulte, J.A., II, Larson, A., Fang, Z., Wang, Y., Tuniyev, B.S. & Papenfuss, T.J. (1998) Phylogenetic relationships of toads in the Bufo bufo species group from the eastern escarpment of the Tibetan Plateau: A case of vicariance and dispersal. Molecular Phylogenetics and Evolution, 9, 80–87.
Matsui, M., Hamidy, A., Murphy, R.W., Khonsue, W., Yambun, P., Shimada, T., Ahmad, N., Belabut, D.M. & Jiang, J. (2010) Phylogenetic relationships of megophryid frogs of the genus Leptobrachium (Amphibia, Anura) as revealed by mtDNA gene sequences. Molecular Phylogenetics and Evolution, 56, 259–272.
Morgan, M.J., Roberts, J.D. & Keogh, J.S. (2007) Molecular phylogenetic dating supports an ancient endemic speciation model in Australia’s biodiversity hotspot. Molecular Phylogenetics and Evolution, 44, 371–385.
Morton, S.R., Short, J. & Barker, R.D. (1995) Refugia for Biological Diversity in Arid and Semi-arid Australia. Biodiversity Series, Paper No. 4, Biodiversity Unit, Dept of Environment, Water, Heritage and the Arts, Commonwealth of Australia. Available from: www.environment.gov.au/biodiversity/publications/series/paper4/index.html (accessed Sept. 8 2010).
Parker, H.W. (1940) The Australasian frogs of the family Leptodactylidae. Novitates Zoologicae, 42, 1–106.
Noonan, B.P. & Chippindale, P.T. (2006) Vicariant origin of Malagasy reptiles supports late Cretaceous Antarctic land bridge. The American Naturalist, 168, 730–741.
Pepper, M., Doughty, P. & Keogh, J.S. (2006) Molecular phylogeny and phylogeography of the Australian Diplodactylus stenodactylus (Gekkota; Reptilia) species-group based on mitochondrial and nuclear genes reveals and ancient split between Pilbara and non-Pilbara D. stenodactylus. Molecular Phylogenetics and Evolution, 41, 539–555.
Rabosky, D.L., Donnellan, S.C., Talaba, A.M. & Lovette, I.J. (2007) Exceptional among-lineage variation in diversification rates during the radiation of Australia’s most diverse vertebrate clade. Proceedings of the Royal Society, Series B, 274, 2915–2923.
Powney, G.D., Grenyer, R., Orme, C.D.L., Owens, I.P.F. & Meiri, S. (2010) Hot, dry and different: Australian lizard richness is unlike that of mammals, amphibians and birds. Global Ecology and Biogeography, 19(3), 386–396.
Raxworthy, C.J., Pearson, R.G., Zimkus, B.M., Reddy, S., Deo, A.J., Nussbaum, R.A. & Ingram, C.M. (2008) Continental speciation in the tropics: contrasting biogeographic patterns of divergence in the Uroplatus leaf-tailed gecko radiation of Madagascar. Journal of Zoology, 275, 423–440.
Read, K., Keogh, J.S., Scott, I.A.W., Roberts, J.D. & Doughty, P. (2001) Molecular phylogeny of the Australian frog genera Crinia, Geocrinia, and allied taxa (Anura: Myobatrachidae). Molecular Phylogenetics and Evolution, 21, 294–308.
Roberts, J.D. & Watson, G.F. (1993) Biography and Phylogeny of the Anura. In: Glasby, C.J., Ross, G.J.B, & Beesley, P.L. Fauna of Australia. Vol. 2A Amphibia and Reptilia. Australian Government Publishing Service; Canberra, pp. 35–40.
San Mauro, D., Gower, D.J., Oommen, O.V., Wilkinson, M. & Zardoya, R. (2004) Phylogeny of caecilian amphibians (Gymnophiona) based on complete mitochondrial genomes and nuclear RAG1. Molecular Phylogenetics and Evolution, 33, 413–427.
Slatyer, C., Rosauer, D. & Lemkert, F. (2007) An assessment of endemism and species richness patterns in the Australian Anura. Journal of Biogeography, 34, 583–596.
Smith, L.A. & Adams, M. (2007) Revision of Lerista muelleri species-group (Lacertilia: Scincidae) in Western Australia, with a redescription of L. muelleri (Fischer, 1881) and the description of nine new species. Records of the Western Australian Museum, 23, 309–357.
Swofford, D.L. (2003) Paup* v4.0b (Computer Program), Sinauer Associates, Sunderland, MA.
Thome, M.T.C., Zamudio, K.R., Giovanelli, J.G.R., Haddad, C.F.B., Baldissera Jr., F.A. & Alexandrino, J. (2010) Phylogeography of endemic toads and post-Pliocence persistence of the Brazilian Atlantic Forest. Molecular Phylogenetics and Evolution, 55, 1018–1031.
Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Computational Biology, 22(22), 4673–4680.
Thompson, G.G., Withers, P.C., McMaster, K.A. & Cartledge, V.A. (2005) Burrows of desert-adapted frogs, Neobatrachus aquilonius and Notaden nichollsi. Journal of the Royal Society of Western Australia, 88, 17–23.
Toon, A., Hughes, J.M. & Joseph, L. (2010) Multilocus analysis of honeyeaters (Aves: Meliphagidae) highlights spatio-temporal heterogeneity in the influence of biogeographic barriers in the Australian monsoonal zone. Molecular Ecology, 18, 2980–2994.
Tyler, M.H., Crook, G.A. & Davies, M. (1983) Reproductive Biology of the frogs of the Magela Creek system, Northern Territory. Records of the South Australian Museum, 18(18), 415–440.
Tyler, M.J., Davies, M. & Martin, A.A. (1981) Australian frogs of the Leptodactylid Genus Uperoleia Gray. Australian Journal of Zoology Supplementary Series, 79, 1–64.
Tyler, M.J. & Doughty, P. (2009) Frogs of Western Australia. 4th Ed. Western Australian Museum, 158 pp.
van der Meijden, A., Vences, M., Hoegg, S. & Meyer, A. (2005) A previously unrecognized radiation of ranid frogs in Southern Africa revealed by nuclear and mitochondrial DNA sequences. Molecular Phylogenetics and Evolution, 37, 674–685.
Van Kranendonk, M.J., Hickman, H.A., Smithies, R.H. & Nelson, D.R. (2002) Geology and tectonic evolution of the Archean North Pilbara terrain, Pilbara Craton, Western Australia. Economic Geology, 67, 695–732.
Vietes, D.R., Min, M. & Wake, D.B. (2004) Rapid diversification and dispersal during periods of global warming by plethodontid salamanders. Proceedings of National Academy of Sciences of the United States of America, 104(50), 19903–19907.