Abstract
Undescribed females representing four morphological types were found in a collection of adult Diamesa from about 5000 m altitude in Rongbuk, Tibet. Short DNA sequences of cytochrome oxidase subunit 2 were used to associate two single males in the material with conspecific females. Diamesa solhoyi n.sp. and Diamesa aculeata n.sp. are described. The complete type material and additional specimens have been deposited in the Insect Collection at the Institute of Zoology, Academia Sinica, Beijing (IZAS). The sequences are deposited in Genbank with accession numbers AM051227–AM051233.References
Ekrem, T. & Willassen, E. (2004) Exploring Tanytarsini relationships (Diptera: Chironomidae) using mitochondrial COII gene sequences. Insect Systematics & Evolution, 35, 263–276.
Flory, E.A. & Milner, A.M. (2000) Macroinvertebrate community succession in Wolf Point Creek, Glacier Bay National Park, Alaska. Freshwater Biology, 44, 465–480.
Gibbs, R.A. & Cockerill, M. (1995) Working on the assembly line. Sequencher for the Macintosh (Version 3.0, March, 1995). Trends in Biochemical Sciences, 20, 162–163.
Hebert, P.D. N., Ratnasingham, S., & deWaard, J.R. (2003) Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species. Proceedings of the Royal Society of London Series B-Biological Sciences, 270, S96–S99.
Kaul, B.K. (1970) Torrenicole insects of the Himalaya: II. Two new Diamesini (Diptera, Chironomidae) from the northwest Himalaya. Oriental Insects, 4, 293–297.
Koshima, S. (1984) A novel cold-tolerant insect found in a Himalayan glacier. Nature, 310, 225–227.
Lods-Crozet, B., Lencioni, V., Olafsson, J.S., Snook, D.L., Velle, G., Brittain, J.E., Castella, E., & Rossaro, B. (2001) Chironomid (Diptera : Chironomidae) communities in six European glacier-fed streams. Freshwater Biology, 46, 1791–1809.
Makarchenko, E.A. (1989) Novyï i maloizvestiyï vidy roda Diamesa (Diptera, Chironomidae) iz Tadzhikistana. Vestnik Zoologii, 2, 82–85.
Makarchenko, E.A. & Bulgakov, G.P. (1986) Novyï vid Diamesa Mg. (Diptera, Chironomidae) iz Uzbekistana. Biologiya vnutrennikh vod, 70, 37–42.
Makarchenko, E.A. & Kobayashi, T. (1997) Diamesa amanoi sp. n., a new species of Diamesinae (Diptera, Chironomidae) from Nepal, with notes on taxonomy and distribution of some Diamesa Meigen. Medical Entomology and Zoology, 48, 45–48.
Reiss, F. (1968) Neue Chironomiden-Arten (Diptera) aus Nepal. Khumbu Himal, 3, 55–73.
Sæther, O.A. (1968) Chironomids from the Finse area, Norway, with special reference to their distribution in a glacier brook. Archiv für Hydrobiologie, 64, 426-483.
Sæther, O.A. (1980) Glossary of chironomid morphology terminology (Chironomidae: Diptera). Entomologica Scandinavica Supplement, 14, 1–51.
Sæther, O.A. & Willassen, E. (1987) Four new species of Diamesa Meigen, 1835 (Diptera, Chironomidae) from the glaciers of Nepal. Entomologica Scandinavica Supplement, 29, 189–203.
Serra-Tosio, B. (1983) Nouveaux Diamesinae de la Paléarctide méridionale et orientale (Diptera, Chironomidae). Spixiana, 6, 1–26.
Shilova, A. (1978) Novaya nakhodka Diamesa lavillei (Diptera,Chironomidae) v faune Sovetskogo Soyuza. Zoologicheskii Zhurnal, 57, 142–143.
Singh, S. (1958) Entomological survey of the Himalaya. Part XXIX. On a collection of nival Chironomidae (Diptera) from the north-west Himalaya. Proceedings of the Indian National Science Academy, 28 (Sec.B), 308–314.
Steffan, A.W. (1971) Chironomid biocoenoses in Scandinavian glacier brooks. Canadian Entomologist, 103, 477–486.
Steffan, A.W. (1974) Die Lebensgemeinschaft der Gletscherbach-zuckmücken (Diptera-Chironomidae) – eine Extrembiozönose. Entomologisk Tidskrift, Supplement, 95, 225–232.
Sunnucks, P. & Hales, D.F. (1996) Numerous transposed sequences of mitochondrial cytochrome oxidase I-II in aphids of the genus Sitobion (Hemiptera: Aphididae). Molecular Biology and Evolution, 13, 510–524.
Swofford, D.L. (2002) PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4. Sinauer Associates, Sunderland, Massachusetts
Tokunaga, M. (1966) Some nematocerous Diptera of the northeast of Afghanistan. Results of Kyoto University Scientific Expedition to Karakorum and Hindukush, 1955, 273–286.
Willassen, E. & Serra-Tosio, B. (1988) Description de trois femelles de Diamesa Meigen (Diptera, Chironomidae), dont D. cinerella Meigen (lectotype et paralectotype). Spixiana Supplement, 14, 91–100.