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Type: Articles
Published: 2011-06-29
Page range: 55-56
Abstract views: 65
PDF downloaded: 38

It’s barcoding Jim, but not as we know it

Department of Ecology, Faculty of Agriculture and Life Sciences, PO Box 84, Lincoln University, Lincoln 7647, Christchurch, New Zealand
Department of Food Science, Faculty of Life Sciences, Chemometrics and Spectroscopy Group, Quality and Technology, University of Copenhagen, Rolighedsvej 30, DK-1958 Frederiksberg C, Denmark
General barcoding Jim

Abstract

It has long been the dream of many ecologists that one day it will be possible to use a hand-held machine to identify biological specimens in the field (e.g. Janzen 2004). An analogy has been made with the so-called “tricorder” from the popular science fiction televison series Star Trek (Savolainen et al. 2005). This idea has arisen largely from the DNA barcoding community, who promote the use of a single universal DNA sequence (usually the mitochondrial cytochrome oxidase I (COI) gene) for species identification (Hebert et al. 2003). Such a device is typically imagined as using DNA as the basis for species determination. However, critics of this idea (e.g. Cameron et al. 2006) have argued that a DNA-based device would be impractical as tissue samples would need to be obtained as a source of DNA, which would necessitate handling the specimen. This suggests that the tricorder may not be such a good analogy; whereas, in Star Trek, the crew of the USS Enterprise merely had to point their tricorders at the organism in question, in reality, field-workers using a DNA-based system would have to obtain a tissue sample and load it into the machine in order to identify their specimen (Cameron et al. 2006, p.844).

References

  1. Cameron, S., Rubinoff, D. & Will, K. (2006) Who will actually use DNA barcoding and what will it cost? Systematic Biology, 55, 844–847.

    Hebert, P.D.N., Cywinska, A., Ball, S.L. & deWaard, J.R. (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society of London, Series B, Biological Sciences, 270, 313–321.

    Janzen, D.H. (2004) Now is the time. Philosophical Transactions of the Royal Society of London, Series B, Biological Sciences, 359, 731–732.

    Munck, L., Møller Jespersen, B., Rinnan, Å., Fast Seefeldt, H., Møller Engelsen, M. & Balling Engelsen, S.A. (2010) Physiochemical theory on the applicability of soft mathematical models – experimentally interpreted. Journal of Chemometrics, 24, 481–495.

    Rodríguez-Fernández, J.I., De Carvalho, C.J.B., Pasquini, C., De Lima, K.M.G., Moura, M. & Arízaga, G.C.G. (2011) Barcoding without DNA? Species identification using near infrared spectroscopy. Zootaxa, 2933, 46–54.

    Savolainen, V., Cowan, R.S., Vogler, A.P., Roderick, G.K. & Lane, R. (2005) Towards writing the encyclopedia of life: an introduction to DNA barcoding. Philosophical Transactions of the Royal Society of London, Series B, Biological Sciences, 360, 1805–1811.