Abstract
We describe a new species of salamander, Paramesotriton zhijinensis, from Guizhou Province, China. The generic allocation of the new species is based on morphological and molecular characters. In morphology, it is most similar to Paramesotriton chinensis but differs in having distinct gland emitting a malodorous secretion (here named scent gland), a postocular stripe, and two non-continuous, dorsolateral stripes on the dorsolateral ridges. Furthermore, neoteny was observed in most individuals of the new species. This has not been previously reported to occur in any other species of Paramesotriton. Analysis of our molecular data suggests that this species a third major evolutionary lineage in the genus Paramesotriton.References
Chan, L.M., Zamudio, K.R. & Wake, D.B. (2001) Relationships of the salamandrid genera Paramesotriton, Pachytriton, and Cynops based on mitochondrial DNA sequences. Copeia, 997–1009.
Fei, L., Hu, S.-Q., Ye, C.Y. & Huang Y.-Z. et al. (2006) Fauna Sinica. Amphibia Vol. 1- General accounts of Amphibia: Gymnophiona and Urodela. Science Press, Beijing, China, 294–313.
Fei, L., Ye, C.Y., Jiang, J.P., Xie, F. & Huang, Y.-Z. (2005) An Illustrated Key to Chinese Amphibians. Sichuan Publishing Group and Sichuan Publishing House of Science and Technology, Chengdu, China.
Kocher, T.D., Thomas, W.K., Meyer, A., Edwards, S.V., Paabo, S., Villablanca, F.X. & Wilson, A.C. (1989) Dynamics of mitochondrial DNA evolution in animals: Amplification and sequencing with conserved primers. Proceedings of the National Academy of Science of the U.S.A., 86, 6196–6200.
Liu, C.-C. & Hu, S.-Q. (1961) Tailless Amphibians of China. Science Press, Peking, China.
Lu, S., Yuan, Z., Pang, J., Yang, D., Yu, F., McGuire, P., Xie, F. & Zhang, Y.P. (2004) Molecular phylogeny of the genus Paramesotriton (Caudata: Salamandridae). Biochemical Genetics, 42, 139–148.
Posada, D. & Buckley, T. R. (2004) Model selection and model averaging in phylogenetics: advantages of the AIC and Bayesian approaches over likelihood ratio tests. Systematic Biology, 53, 793–808.
Ronquist, F.R. & Huelsenbeck, J.P. (2003) MRBAYES: Bayesian inference of phylogeny. Bioinformatics, 19, 1572–1574.
Stuart, B.L. & Papenfuss, T.J. (2002) A new salamander of the genus Paramesotriton (Caudata: Salamandridae) from Laos. Journal of Herpetology, 36, 145–148.
Swofford, D.L. (2003) PAUP: Phylogenetic Analysis Using Parsimony (and Other Methods).Version 4 0b10 Sinauer Associates, Sunderland, MA.
Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. & Higgins, D.G. (1997) The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 24, 4876–4882.
Weisrock, D.W., Papenfuss, T.J., Macey, J.R., Litvinchuk, S.N., Polymeni, R., Ugurtas, I.H., Zhao, E., Jowkar, H. & Larson, A. (2006) A molecular assessment of phylogenetic relationships and lineage accumulation rates within the family Salamandridae (Amphibia, Caudata). Molecular Phylogenetics and Evolution, 41, 368–383.