Abstract
We describe a new species of chameleon, Trioceros kinangopensis sp. nov., from Kinangop Peak in the Aberdare moun-tains, central highlands of Kenya. The proposed new species is morphologically and genetically distinct from other mem-ber of the bitaeniatus-group. It is morphologically most similar to T. schubotzi but differs in the lack of sexual sizedimorphism, smaller-sized females, smoother, less angular canthus rostrales, smaller scales on the temporal region and abright orange gular crest in males. Mitochondrial DNA indicates that the proposed new taxon is a distinct lineage that isclosely related to T. nyirit and T. schubotzi. The distribution of T. kinangopensis sp. nov. appears to be restricted to the afroalpine zone in vicintiy of Kinangop Peak and fires may pose a serious threat to the long-term survival of this species.References
Albert, E.M., Zardoya, R. & Garcia-Paris, M. (2007) Phylogeographical and speciation patterns in subterranean worm lizards of the genus Blanus (Amphisbaenia: Blanidae). Molecular Ecology, 16, 1519–1531.
Andren, C. (1976) The reptile fauna in the lower alpine zone of Aberdare Mountains and Mt. Kenya. British Journal of Herpetology, 5, 566–575.
Ballard, J.W.O. & Whittock, M.C. (2004) The incomplete natural history of mitochondria. Molecular Ecology, 13, 729–744.
Barej, M.F., Ineich, I., Gvoždík, V., Lhermitte-Vallarino, N., Gonwouo, N.L., LeBreton, M., Bott, U. & Schmitz, A. (2010) Insights into chameleon of the genus Trioceros (Squamata: Chamaeleonidae) in Cameroon with ressurection of Chamaeleon serratus Mertens 1922. Bonn Zoological Bulletin, 57(2), 211–229.
Ben-Moshe, A., Dayan, T. & Simberloff, D. (2001) Convergence in morphological patterns and cmmunity organisation between old and new world rodent guilds. American Naturalist, 158(5), 484–495.
Bickel, R. & Losos, J.B. (2002) Patterns of morphological variation and correlates of habitat use in chameleons. Biological Journal of the Linnean Society, 76, 91–103.
Boumans, L., Vietes, D.R., Glaw, F. & Vences, M. (2007) Geographical patterns of deep mitochondrial differentiation in widespread Malagasy reptiles. Molecular Phylogenentics and Evolution, 45, 822–839.
Brandley, M.C., Schmitz, A. & Reeder, T. (2005) Partitioned Bayesian analysis, partition choice, and the phylogenetic relationships of Scincid lizards. Systematic Biology, 54(3), 373–390.
Broadley, D.G. & Howell, K.M. (1991) A check list of the reptiles of Tanzania, with synoptic keys. Syntarsus, 1, 1–70.
Brown, J.M. & Lemon, A.R. (2007) The importance of data partitioning and utility of Bayes factors in Bayesian phylogenetics. Systematic Biology, 56(4), 643–655.
Carranza, S., Arnold, E.N., Geniez, Ph., Roca, J. & Mateo, J.A. (2008) Radiation, multiple dispersal and parallelism in the skinks, Chalcides and Sphenops (Squamata: Scincidae), with comments on Scincus and Scincopus and the age of the Sahara Desert. Molecular Phylogenetics and Evolution, 46, 1071–1094.
Coe, M.J. (1967) The Ecology of the Alpine Zone of Mount Kenya. Junk, The Hague.
Cracraft, J. (1983) Species concepts and speciation analysis. Current Ornithology, 1, 159–187.
de Quieroz, K. (1998) The general lineage concept of species, species criteria, and the process of speciation: A conceptual unification and terminological recommendations. In: Howard, D.J. & Berlocher, S.H.(eds). Endless Forms. Species and Speciation. Oxford University Press, England. pp. 57–75.
Felsenstein, J. (1985) Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39, 783–791.
Felsenstein, J. (2004) Inferring Phylogenies. Sinauer Associates, Sunderland, Mass.
Forstner, M.R.J., Davis, S.K. & Arevalo, E. (1995) Support for the hypothesis of anguimorph ancestry for the suborder Serpentes from phylogenetic analysis of mitochondrial DNA sequences. Molecular Phylogenetics and Evolution, 4, 93–102.
Fu, J. (2000) Toward the phylogeny of the family Lacertidae – why 4708 base pairs of mtDNA sequences cannot draw the picture. Biological Journal of the Linnean Society, 71, 203–217.
Gehring, P.S., Pabijan, M., Ratsoavina, M., Köhler, J., Vences, M. & Glaw, F. (2010) A Tarzan yell for conservation: a new chameleon, Calumma tarzan sp. n., proposed as a flagship species for the creation of new nature reserves in Madagascar. Salamandra, 46(3), 167–179.
Glaw, F., Köhler, J., Townsend, T.M. & Vences, M. (2012) Rivaling the world’s smallest reptiles: discovery of miniaturized and microendemic new species of leaf chameleons (Brookesia) from northern Madagascar. PLoS ONE, 7(2): e31314. doi:10.1371/journal.pone.0031314.
Gompert, Z., Forister, M.L., Fordyces, J.A. & Nice, C.C. (2008) Widespread mito-nuclear discordance with evidence for introgressive hybrisation and selective sweeps in Lycacides. Molecular Ecology, 17, 5231–5244.
Hailer, F., Kutschera, V.E., Hallstrom, B. M., Klassert, D., Fain, S. R., Leonard, J.A., Arnason, U & Janke, A. (2012) Nuclear genomic sequences reveal that polar bears are an old and distinct bear lineage. Science, 336, 344–347.
Hillis, D.M. & Bull, J.J. (1993) An empirical test of boot strapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology, 42, 192–192
Honda, M., Hidetoshi, O., Köhler, G., Ineich, I., Chorio, L., Chen, S. & Hikida, T. (2003) Phylogeny of the lizard subfamily Lygosominae (Reptilia: Scincidae), with special reference to the origin of the New World taxa. Genes & Genetic Systems, 78, 71–80.
Hopkins, K.P. & Tolley, K.A. (2011) Morphological variation in the Cape dwarf chameleon (Bradypodion pumilum) as a consequence of spatially explicit habitat structure differences. Biological Journal of the Linnean Society, 102, 878–888.
Huelsenbeck, J. P. & Rannala, B. (2004) Frequentist properties of Bayesian posterior probabilities of phylogenetic trees under simple and complex substitution models. Systematic Biology, 53(6), 904–913.
IUCN Standards and Petitions Subcommittee (2010) Guidelines for Using the IUCN Red List Categories and Criteria. Version 8.1. Prepared by the Standards and Petitions Subcommittee in March 2010. Available from
http://intranet.iucn.org/webfiles/doc/SSC/RedList/RedListGuidelines.pdf (Accessed 2 May 2011).
Karr, J.R. & James, F.C. (1975) Eco-morphological configurations and convergent evolution of species and communities. In: Cody, M.L. & Diamond, J.M. (eds) Ecology and Evolution of Communities. Cambridge, MA: Harvard University Press. pp. 258–291.
Kawakita, A., Sota, T., Ascher, J.S., Ito, M., Tanaka, H. & Kato, M. (2003) Evolution and phylogenetic utility of alignment gaps within intron sequences of three nuclear genes in Bumble Bees (Bombus). Molecular Biology and Evolution, 20(1), 87–92.
Klaver, C. & Böhme, W. (1997) Chamaeleonidae. Das Tierreich, 112, 1–67.
Koreny, L. (2006) Phylogeny of East-African chameleons. MSc thesis. Faculty of Biological Sciences, University of Bohemia in Ceske Budejovice. April 2006. pp36.
Krause, P. & Böhme, W. (2010) A new chameleon of the Trioceros bitaeniatus group from Mt. Hanang, Tanzania, East Africa (Squamata: Chamaelonidae). Bonner Zoologische Beiträge, 57(1), 19–29.
Leache, A.D. & McGuire, J.A. (2006) Phylogenetic relationships of horned lizards (Phrynosoma) based on nuclear and mitochondrial data: evidence for a misleading mitochondrial gene tree. Molecular Phylogenetics and Evolution, 39, 628–644.
Losos, J.B. (2009) Lizards in an evolutionary tree. Ecology and adaptive radiation of Anoles. University of California Press.
Loveridge, A. (1957) Checklist of the reptiles and amphibians of East Africa (Uganda; Kenya; Tanganyika; Zanzibar). Bulletin of the Museum of Comparative Zoology, Harvard, 117, 153–362.
Lutzmann, N., Stipala, J., Lademann, R., Krause, P., Wilms, T. & Schmitz, A. (2010) Description of a new subspecies of Kinyongia uthmoelleri (Müller, 1938) (Squamata: Chamaeleonidae) with notes on its captive propagation. Bonner Zoologische Beiträge, 57(2), 281–288.
Luxbacher, A.M. & Knouft, J.H. (2009) Assessing concurrent patterns of environmental niche and morphological evolution among species of horned lizards (Phrynosoma). Journal of Evolutionary Biology, 22, 1669–1678.
Mariaux, J. & Tilbury, C.R. (2006) The pygmy chameleons of the eastern arc range (Tanzania): evolutionary relationships and the description of three new species of Rhampholeon (Sauria: Chamaeleonidae). Herpetological Journal, 16, 315–331.
Mariaux, J., Lutzmann, N. & Stipala, J. (2008) The two-horned chameleons of East Africa. Zoological Journal of the Linnean Society, 152, 367–391.
Matthee, C.A., Tilbury, C.R. & Townsend, T. (2004) A phylogenetic review of the African leaf chameleons: genus Rhampholeon (Chamaeleonidae): the role of vicariance and climate change in speciation. Proceedings of the Royal Society, London B, 271(1551), 1967–1975.
Measey, G.J. & Tolley, K.A. (2011) Sequential fragmentation of Pleistocene forests in an East Africa biodiversity hotspot: chameleons as a model to track forest history. PLoS ONE, 6(10), e26606. doi:10.1371/journal.pone.0026606.
Menegon, M. Salvidio, S. & Tilbury, C. (2002) A new dwarf forest chameleon from the Udzungwa Mountains of Tanzania, East Africa (Squamata: Rhampholeon Günther, 1874). Journal of Herpetology, 36, 51–57.
Menegon, M., Tolley, K.A., Jones, T., Rovero, F., Marshall, A.R. & Tilbury, C.R. (2009) A new species of chameleon (Sauria: Chamaeleonidae: Kinyongia) from the Magombera forest and the Udzungwa Mountains National Park, Tanzania. African Journal of Herpetology, 58(2), 59–70.
Mertens, R. (1966) Chamaeleonidae. Das Tierreich, 83, 1–37.
Necas, P. (1999) Chameleons - Nature's hidden jewels. Kreiger Publishing.
Necas, P. (2009) Ein neues Chamäleon der Gattung Kinyongia Tilbury, Tolley & Branch 2006 aus den Poroto-Bergen, Süd-Tansania (Reptilia: Sauria: Chamaeleonidae). Sauria, 31(2), 41–48.
Necas, P., Modry, D. & Slapeta, J.R. (2003) Chamaeleo (Trioceros) narraioca n. sp. (Reptilia Chamaeleonidae), a new chameleon species from a relict montane forest of Mount Kulal, northern Kenya. Tropical Zoology, 16(1), 1–12.
Necas, P., Modry, D. & Slapeta, J.R. (2005) Chamaeleo (Trioceros) ntunte sp. n., a new chameleon species from Mt. Nyiru, northern Kenya. Herpetozoa, 18(3/4), 125–132.
Necas, P., Sindaco, R., Koreny, L., Kopecna, J., Malonza, P. & Modry, D. (2009) Kinyongia asheorum sp. n., a new montane chameleon from the Nyiru Range, northern Kenya (Squamata: Chamaeleonidae). Zootaxa, 2028, 41–50.
Nylander, J.A.A., Ronquist, R., Huelsenbeck, J.P. & Nieves-Aldrey, J.L. (2004) Bayesian phylogenetic analysis of combined data. Systematic Biology, 53(1), 47–67.
Palumbi, S.R., Martin, A., Romano, S., McMillan, W.O., Stice, L. & Grabowski, G. (1991) The simple fool’s guide to PCR. Department of Zoology, University of Hawaii, Honolulu.
Rambaut, A. & Drummond, A.J. (2007). Tracer. Version 1.4. (Accessed 18 May 2009).
Rand, A.S. (1963). Notes on the Chamaeleo bitaeniatus complex. Bulletin of the Museum of Comparative Zoology, Harvard, 130(1), 1–29.
Rato, C., Carranza, S., Perera, A., Carretero, M.A. & Harris, D. J. (2010) Conflicting patterns of nucleotide diversity between mtDNA and nDNA in the Moorish gecko, Tarentola mauritanica. Molecular Phylogenetics and Evolution, 56, 962–971.
Raxworthy, C.J., Forstner, M.R. & Nussbaum, R.A. (2002) Chameleon radiation by oceanic dispersal. Nature, 415, 784–787.
Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes version 3.0: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19(12), 1572–1574.
Ronquist, F., Huelsenbeck, J.P. & van der Mark, P. (2005) MrBayes 3.1 Manual. 'Draft 26/05/2005'. Available at: http://mrbayes.csit.fsu.edu/manual.php (Accessed 1 May 2005).
Sequiera, F., Alexandrino, J., Weiss, S. & Ferrand, N. (2008) Documenting the advantages and limitations of different classes of molecular markers in a well-established phylogeographic context: lessons from the Iberian endemic Golden-striped salamander Chioglossa lusitanica (Caudata: Salamandridae). Biological Journal of the Linnean Society, 95, 371–387.
Spawls, S., Howell, K., Drewes, R. & Ashe, J. (2002) A Field Guide to the Reptiles of East Africa. Academic Press, London.
Stipala, J., Lutzmann, N., Malonza, P.K., Borghesio, L., Wilkinson, P., Godley, B. & Evans, M.R. (2011) A new species of chameleon (Sauria: Chamaeleonidae) from the highlands of northwest Kenya. Zootaxa, 3002, 1–16.
Stothard, P. (2000) The sequence manipulation suite: JavaScript programs for analyzing and formatting protein and DNA sequences. Biotechniques, 28, 1102–1104.
Stuart-Fox, D. & Moussalli, A. (2007) Sex-specific ecomorphological variation and the evolution of sexual dimorphism in dwarf chameleons (Bradypodion spp.). Journal of Evolutionary Biology, 20, 1073–1081.
Swofford, D.L. (2002) PAUP - Phylogenetic Analysis Using Parsimony (and Other Methods). Beta Version 4.0b10. Sinauer, Sunderland.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., & Kumar, S. (2011) MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution. doi: 10.1093/molbev/msr121.
Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673–4680.
Tilbury, C.R. (1991) A new species of Chamaeleo Laurenti 1768 (Reptilia Chamaeleonidae) from a relict montane forest in northern Kenya. Tropical Zoology, 4, 159–165.
Tilbury, C.R. (1998) Two new chameleons (Sauria: Chamaeleonidae) from isolated afromontane forests in Sudan and Ethiopia. Bonner Zoologische Beiträge, 47(3/4), 293–299.
Tilbury, C.R. (2010) Chameleons of Africa – An Atlas Including the Chameleons of Europe, the Middle East and Asia. Chimaira Publications, Frankfurt am Main.
Tilbury, C.R. & Tolley, K.A. (2009) A re-appraisal of the systematics of the African genus Chamaeleo (Reptilia: Chamaeleonidae). Zootaxa, 2079, 57–68.
Tolley, K.A., Tilbury, C.R., Branch, W.R. & Matthee, C.A. (2004) Phylogenetics of the Southern African dwarf chameleons, Bradypodion (Squamata: Chamaeleonidae). Molecular Phylogenetics and Evolution, 30, 354–365.
Tolley, K.A., Tilbury, C.R., Measey, G.J., Menegon, M., Branch, W.R. & Matthee, C.A. (2011) Ancient forest fragmentation or recent radiation? Testing refugial speciation models in chameleons within an African biodiversity hotspot. Journal of Biogeography, 38(9), 1748–1760.
Townsend, T. & Larson, A. (2002) Molecular phylogenetics and mitochondrial genomic evolution in the Chamaeleonidae (Reptilia, Squamata). Molecular Phylogenetics and Evolution, 23(1), 22–36.
Werner, F. (1911) Chamaeleontidae. Das Tierreich, 27, ii–52.
Wiens, J.A. (1991) Ecomorphological comparisons of the shrub-desert avifaunas of Australia and North America. Oikos, 60, 55–63.
Winemiller, K.O., Kelso-Winemiller, L.C. & Brenkert, A.L. (1995) Ecomorphological diversification and convergence in fluvial cichlid fishes. Environmental Biology of Fishes, 44, 235–261.
de Witte, G. (1965) Les cameleons de l'Afrique centrale. Annales Musee Royal de l'Afrique Centrale, Tervuren. série 8 Sciences, Zoologie, 142, 1–215.
Wuster, W., Peppin, L., Pook, C.E. & Walker, D.E. (2008) A nesting of vipers: Phylogeny and historical biogeography of the Viperidae (Squamata: Serpentes). Molecular Phylogenetics and Evolution, 49, 445–459.
Zhang, D.X. & Hewitt, G.M. (1996) Nuclear integrations: challenges for mitochondrial DNA markers. Trends in Ecology and Evolution, 11, 247–251.