Abstract
A new technique is presented that allows mounting insects directly from alcohol storage, while still wet or soaked, withoutany chemical treatment. It works particularly well for many groups of Hymenoptera, but not with delicate or softspecimens. Preliminary results are also commented for some Coleoptera, Diptera, Hemiptera, Mantodea, Neuroptera, andOrthoptera. External drying is performed with the aid of a small electric air pump. A 600 L/min air current quickly andefficiently spreads the specimen’s wings and pilosity, while helping to position the antennae, legs, and abdomen.Specimens need to be pinned, even if small and pinned with minutens; this generally means the new technique will workfor any insect greater than 3 mm long. This is precisely the size range for which an efficient mounting aid for alcohol preserved specimens was lacking.References
Adis, J., Basset, Y., Floren, A., Hammnod, P.M. & Linsenmair, K.E. (1998) Canopy fogging of an overstory tree – recommendations for standardization. Ecotropica, 4, 93–97.
Aguiar, A.P. & Santos, B.F. (2010) Discovery of potent, unsuspected sampling disparities for Malaise and Möricke traps, as shown for Neotropical Cryptini (Hymenoptera, Ichneumonidae). Journal of Insect Conservation, 14, 199–206.
Brown, B.V. (1993) A further chemical alternative to critical-point-drying for preparing small (or large) flies. Fly Times, 11, 10.
Dillon, N., Austin, A.D. & Bartowsky, E. (1996) Comparison of preservation techniques for DNA extraction from hymenopterous insects. Insect Molecular Biology, 5, 21–24.
Fisher, R.W. & Jursic, F. (1958) Rearing houseflies and roaches for physiological research. The Canadian Entomologist, 90, 1–7.
Gordh, G. & Hall, J.C. (1979) Critical point drier used as a method of mounting insects from alcohol. Entomological News, 90, 57–59. Available at http://biostor.org/reference/77276
Hanson, P.E. & Gauld, I.D. (Eds) (1995) The Hymenoptera of Costa Rica. New York: Oxford University Press and The Natural History Museum, London.
Jenkins, J. (1991) A simple device to clean insect specimens for museums and scanning electron microscopy. Proceedings of the Entomological Society of Washington, 93, 204–205.
Martin, J.E.H. (1977) Collecting, Preparing, and Preserving Insects, Mites, and Spiders. The Insects and Arachnids of Canada. Part 1. Publication 1643. 182 pp.
Noort, S. van (1995) A simple yet effective method for drying alcohol preserved specimens. Chalcid Forum, 18, 3–4. Available at http://www.sel.barc.usda.gov/hym/chalcforum.html (Accessed 26 March 2012).
Noyes, J.S. (1982) Collecting and preserving chalcid wasps (Hymenoptera: Chalcidoidea). Journal of Natural History, 16, 315–334.
Orozco, D. & Gaimari, S. (2012) HMDS (hexamethyldisilizane) Protocol. Available at http://www.cdfa.ca.gov/plant/ppd/entomology/HMDS.html (Accessed 26 March 2012).
Sabrosky, C.W. (1966) Mounting insects from alcohol. Bulletin of the Entomological Society of America, 12(3), 349.
Schauff, M.E. (2005) (Ed) Collecting and preserving insects and mites: Techniques and tools. Edited by M.E.Schauff, National Museum of Natural History, Washington, DC. Available at www.ars.usda.gov (Accessed 26 March 2012).
Taylor, T.L. (1993) Drying Methods for Insects from Alcohol. Chalcid Forum, 16, 10. Available at http://www.sel.barc.usda.gov/hym/chalcforum.html (Accessed 26 March 2012)
Truman, J.W. (1968) Acetone Treatment for Preservation of Adult and Larval Mosquitoes. Annals of the Entomological Society of America, 61(3), 779–780.
Vockeroth, J.R. (1966) A method of mounting insects from alcohol. The Canadian Entomologist, 98, 69–70.