Abstract
A new water mite, Torrenticola lukai n. sp. (Hydrachnidia, Torrenticolidae), is described from running waters in NorthernMontenegro. The new species is probably a sister species of Torrenticola lundbladi (Viets, 1930), a little documented spe-cies known from central Spain, which is redescribed based on recently collected new material. Morphological data hasbeen obtained with a bright field transmission microscopy and Laser Scanning Confocal Microscopy (LSCM), the latterallowing a realistic representation of the animals external morphology. Voucher specimens provided the DNA from whichbarcode sequences were obtained. External morphology and molecular data confirm that both species are clearly distin-guishable and diagnosticable. A key is given for the males of European species characterized by shoulder platelets fused to the dorsal plate.References
Asadi, M., Hinomoto N., Saboori A. & Javan-Nikkhah M. (2012) Genetic diversity in mitochondrial cytochrome c oxidase subunit I sequences of the water mite Hygrobates fluviatilis (Acari: Hydrachnidia: Hygrobatidae. Internatıonal Journal of Acarology, 38 (2), 96–100.
Di Sabatino, A., Gerecke, R., Smit, H., Pesic, V. & Panesar, A. (2003) Water mites of the family Torrenticolidae (Acari, Actinedida, Hydrachnidia) from the Eastern Mediterranean region. Archiv für Hydrobiologie Supplement, 139 (3), 1–39.
Di Sabatino, A., Gerecke R., Gledhill T. & Smit H. (2010) Chelicerata: Acari II. In: Gerecke, R. (Ed), Süßwasserfauna von Mitteleuropa, 7, 2–2: 1–134, Elsevier GmbH, Spektrum Akademischer Verlag, München.
Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–297.
Hebert, P.D.N., Cywinska, A., Ball, S.L. & de Waard, J.R. (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society Biological Sciences Series B, 270 (1512), 313–321.
Lundblad, O. (1941) Neue Wassermilben aus Madeira. Entomologisk Tidskrif, 62, 93–96.
Lundblad, O. (1956) Zur Kenntnis süd- und mitteleuropäischer Hydrachnellen. Arkiv för Zoologi, 10 (1), 1–306.
Martin, P., Dabert, M. & Dabert, J. (2010) Molecular evidence for species separation in the water mite Hygrobates nigromaculatus Lebert, 1879 (Acari, Hydrachnidia): evolutionary consequences of the loss of larval parasitism. Aquatic Sciences, 72, 347–360.
Nixon, K.C. & Carpenter J.M. (1996) On simultaneous analysis. Cladistics 12 (3), 221–241.
Pešić, V. (2004) Some new and rare water mites (Acari: Hydrachnidia) from the Balkan peninsula. Glasnik Republičkog Zavoda za zaštitu prirode i Prirodnjačkog Muzeja, 27–28, 1994–1995 (2004), 93–99.
Pešić, V., Ağirbaş, E.& Turan, D. (2007) A contribution to the knowledge of the water mite fauna of running waters draining to the Eastern Black Sea coast of Turkey. Lauterbornia, 59, 45–52.
Pešić, V., Smit, H., Gerecke, R. & Di Sabatino, A. (2010) The water mites (Acari: Hydrachnidia) of the Balkan peninsula, a revised survey with new records and descriptions of five new taxa. Zootaxa, 2586, 1–100.
Pešić, V. & Smit, H. (2012a) Water mites of the genus Monatractides (Acari: Hydrachnidia, Torrenticolidae) from Australia, with descriptions of four new species. Zootaxa, 3248, 1–24.
Pešić, V. & Smit H. (2012b) Second contribution to the knowledge of water mites of the genus Monatractides K. Viets (Acari: Hydrachnidia, Torrenticolidae) from New Guinea, with descriptions of three new species. Zootaxa, 3350, 46–57.
Pešić, V., Smit H. & Saboori A. (2012) Water mites delineating the Oriental and Palaearctic regions - the unique fauna of southern Iran, with descriptions of one new genus, one new subgenus and 14 new species (Acari: Hydrachnidia). Zootaxa, 3330, 1–67.
Schindelin, J., Arganda-Carreras, Ignacio., Frise, E., Kaynig, Verena., Longair, M., Pietzsch, T., Preibisch, S., Rueden, Curtis., Saalfeld, S., Schmid, B., Tinevez, J-Y., White, D.J., Hartenstein, V., Eliceiri, K., Tomancak P. & Cardona A. (2012) Fiji: an open-source platform for biological-image analysis. Nature Methods, 9 (7), 676–682.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M. & Kumar, S. (2011) MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Molecular Biology and Evolution, 28, 2731–2739.
Valdecasas, A.G. (1998) Lista sinonímica y bibliográfica de las Hidracnelas (Acari, Hydrachnellae) de la Península Ibérica, Islas Baleares e Islas Canarias. Lista de la flora y fauna de las aguas continentales de la peninsula iberica. Publ. N° 5: 1–81. Asociacion Española de Limnologia, Madrid.
Valdecasas, A.G. (2008) Confocal microscopy applied to water mite taxonomy with the description of a new genus of Axonopsinae (Acari, Parasitengona, Hydrachnidia) from Central America. Zootaxa, 1820, 41–48.
Valdecasas, A.G. & Abad, A. (2011) Morphological Confocal Microscopy in Arthropods and the Enhancement of Autofluorescence after Proteinase K Extraction. Microscopy and Microanalysis, 17, 109–113.
Viets, K. (1930) Zur Kenntnis der Hydracarinen-Fauna von Spanien. Archiv für Hydrobiologie, 21 (2), 175–240.
Wiles, P.R. (1997) Asian and Oriental Torrenticolidae Piersig, 1902 (Acari: Hydrachnidia: Lebertioidea): a revision of the family and description of new species of Torrenticola Piersig and Pseudotorrenticola Walter, from Southeast Asia. Journal of Natural History, 31, 191–236.
Zhang, Z-Q., Fan, Q-H, Pešić, V., Smit, H., Bochkov, A.V. , Khaustov, A. A., Baker, A., Wohltmann, A., Wen, T., Amrine, J.W,. Beron, P. Lin, J., Gabrys, G & Husband R (2011). Order Trombidiformes Reuter, 1909. In: Zhang, Z.-Q. (Ed.) Animal biodiversity: An outline of higher-level classification and survey of taxonomic richness. Zootaxa, 3148, 129–138.