Abstract
We review the taxonomic status of microhylid frogs of the genus Platypelis occurring on the Tsaratanana mountain rangein northern Madagascar. Two species have originally been described from the region, Platypelis alticola and P.tsaratananaensis. We found P. alticola at elevations from 1589–2429 m, with the population at 1589 m being geneticallydivergent (pairwise sequence divergence in the 16S rRNA gene fragment 3.8%). The species was mainly found in bamboosegments accessible via small holes, and its vocalization was a tonal single-note call repeated in long regular series. Theidentity of P. tsaratananaensis was uncertain for many years. Based on comparisons with the type material we concludethat the name should be applied to a common species in the Tsaratanana region morphologically similar to P. pollicarisbut distinguished by a double-note advertisement call, repeated in long series in P. tsaratananaensis (vs. single-note in P.pollicaris), relative length of toes, and size of the prepollex in males (larger in P. pollicaris). Populations of P.tsaratananaensis from several nearby forests were genetically similar to those from the main Tsaratanana Massif. Thespecies is nidicolous with 1–27 embryos and non-feeding larvae in waterfilled bamboo segments. Oocyte number iscorrelated with female size, and larger adult specimens were found in bamboo segments situated higher above the ground.A new species, Platypelis olgae sp. nov., is described from two sites at ca. 2500 m elevation on the Tsaratanana, based onits genetic and morphological differentiation. It differs from other Platypelis by small body size (adult snout-vent length20–22 mm) the absence or rudimentary state of vomerine teeth, green to yellow ventral color, and absence of contrastingand sharply delimited symmetrical dark dorsal patches. Platypelis alticola, P. tsaratananaensis and P. olgae sp. nov. were regularly parasitized by mites probably of the genus Endotrombicula.References
AmphibiaWeb: Information on amphibian biology and conservation.[web application] (2012) Berkeley, California: AmphibiaWeb. Available from: <http://amphibiaweb.org.> (Accessed: 22 May 2012).
Andreone, F., Cadle, J.E., Cox, N., Glaw, F., Nussbaum, R.A., Raxworthy, C.J., Stuart, S., Vallan, D. & Vences, M. (2005a) Species review of amphibian extinction risks in Madagascar: conclusions from the Global Amphibian Assessment. Conservation Biology, 19, 1790–1802.
Andreone, F., Carpenter, A.I., Cox, N., du Preez, L., Freeman, K., Furrer, S., García, G., Glaw, F., Glos, J., Knox, D., Köhler, J., Mendelson III, J.R., Mercurio, V., Mittermeier, R.A., Moore, R.D., Rabibisoa, N.H.C., Randriamahazo, H., Randrianasolo, H., Rasomampionona Raminosoa, N., Ravoahangimalala Ramilijaona, O., Raxworthy, C.J., Vallan, D., Vences, M., Vieites, D.R. & Weldon, C. (2008a) The challenge of conserving amphibian megadiversity in Madagascar. PLoS Biology, 6, e118.
Andreone, F., Cox, N., Glaw, F., Köhler, J., Rabibisoa, N.H.C., Randriamahazo, H., Randrianasolo, H., Raxworthy, C.J., Stuart, S.N., Vallan, D. & Vences, M. (2008b) Update of the Global Amphibian Assessment for Madagascar in light of new species discoveries, nomenclature changes, and new field information. In: Andreone, F. (editor): A Conservation Strategy for the Amphibians of Madagascar. Monografie del Museo Regionale di Scienze Naturali di Torino, 45, 419–438.
Andreone, F., Glaw, F., Mattioli, F., Jesu, R., Schimmenti, G., Randrianirina, J.E. & Vences, M. (2009) The peculiar herpetofauna of some Tsaratanana rainforests and its affinities with Manongarivo and other massifs and forests of northern Madagascar. Italian Journal of Zoology, 76, 92–110.
Andreone, F., Vences, M., Vieites, D.R., Glaw, F. & Meyer, A. (2005b) Recurrent ecological adaptations revealed through a molecular analysis of the secretive cophyline frogs of Madagascar. Molecular Phylogenetics and Evolution, 34, 315–322.
Blommers-Schlösser, R.M.A. (1975) Observations on the larval development of some Malagasy frogs, with notes on their ecology and biology (Anura: Dyscophinae, Scaphiophryninae and Cophylinae). Beaufortia, 24, 7–26.
Blommers-Schlösser, R.M.A. & Blanc, C.P. (1991) Amphibiens (première partie). Faune de Madagascar, 75, 1–379.
Blommers-Schlösser, R.M.A. & Blanc, C.P. (1993) Amphibiens (deuxième partie). Faune de Madagascar, 75, 384–530, plates 13–20.
Boulenger, G.A. (1888) Descriptions of new reptiles and batrachians from Madagascar. Annals and Magazine of Natural History (6), 1, 101–107.
Dorr, L.J. (1997) Plant Collectors in Madagascar and the Comoro Islands. Kew. London.
Glaw, F., Köhler, J. & Vences, M. (2012) A tiny new species of Platypelis from the Marojejy National Park in northeastern Madagascar (Amphibia: Microhylidae). European Journal of Taxonomy, 9, 1–9.
Glaw, F. & Vences, M. (1994) A Fieldguide to the Amphibians and Reptiles of Madagascar, Köln, Germany: Vences and Glaw Verlags GbR. 480 pp.
Glaw, F. & Vences, M. (2007) A Field Guide to the Amphibians and Reptiles of Madagascar, 3rd edition. Köln, Germany: Vences and Glaw Verlags GbR, 496 pp.
Gosner, K.L. (1960) A simplified table for stageing anuran embryos and larvae with notes on identification. Herpetologica, 16, 183–190.
Grosjean, S., Glos, J., Teschke, M., Glaw, F. & Vences, M. (2007) Comparative larval morphology of Madagascan toadlets of the genus Scaphiophryne: phylogenetic and taxonomic inferences. Zoological Journal of the Linnean Society, 151, 555–576.
Guibé, J. (1974) Batraciens nouveaux de Madagascar. Bulletin du Museum National de Histoire Naturelle Paris 3rd Series, 171, 1169–1192.
Guibé, J. (1978) Les batraciens de Madagascar. Bonner zoologische Monographien, 11, 1–140.
Köhler, J., Glaw, F. & Vences, M. (1997) Notes on the reproduction of Rhombophryne testudo (Anura: Microhylidae) at Nosy Be, northern Madagascar. Revue française d'Aquariologie et Herpetologie, 24 (1–2), 53–54.
Köhler, J., Vences, M., D'Cruze, N. & Glaw, F. (2010) Giant dwarfs: discovery of a radiation of large-bodied 'stump-toed frogs' from karstic cave environments of northern Madagascar. Journal of Zoology, 282, 21–38.
Nylander, J.A. (2004) MrModeltest v2. Program distributed by the author, Evolutionary Biology Centre, Uppsala University.
Nylander, J.A.A., Wilgenbusch, J.C., Warren, D.L. & Swofford, D.L. (2008) AWTY (are we there yet?): a system for graphical exploration of MCMC convergence in Bayesian phylogenetics. Bioinformatics, 24, 581–583.
Pyron, R.A. & Wiens, J.J. (2011) A large-scale phylogeny of Amphibia including over 2800 species, and a revised classification of extant frogs, salamanders, and caecilians. Molecular Phylogenetics and Evolution, 61, 543–583.
Rakotoarison, A., Rasamison, S.A., Rajeriarion, E., Vieites, D.R. & Vences, M. (2010) Hypotheses on ecological interactions between the aye-aye (Daubentonia madagascariensis) and microhylid frogs of the genus Platypelis in Tsaratanana bamboo forest. Lemur News, 15, 21–23.
Raselimanana, A.P., Raxworthy, C.J. & Nussbaum, R.A. (2000) Herpetofaunal species diversity and elevational distribution within the Parc National de Marojejy, Madagascar. Fieldiana Zoology (N.S.), 97, 157–174.
Ratsoavina, F.M., Louis Jr., E.E., Crottini, A., Randrianiaina, R.D., Glaw, F. & Vences, M. (2011) A new leaf tailed gecko species from northern Madagascar with a preliminary assessment of molecular and morphological variability in the Uroplatus ebenaui group. Zootaxa, 3022, 39–57.
Raxworthy, C.J. & Andreone, F. (2004) Platypelis tsaratananaensis. In: IUCN 2011. IUCN Red List of Threatened Species. Version 2011.2. <www.iucnredlist.org>. Downloaded on 21 May 2012.
Raxworthy, C.J., Pearson, R.G., Rabibisoa, N., Rakotondrazafy, A.M., Ramanamanjato, J.-B., Raselimanana, A.P., Wu, S., Nussbaum, R.A. & Stone, D. (2008) Extinction vulnerability of tropical montane endemism from warming and upslope displacement: a preliminary appraisal for the highest massif in Madagascar. Global Change Biology, 14, 1–18.
Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.
Vences, M., Glaw, F., Köhler, J. & Wollenberg, K.C. (2010) Molecular phylogeny, morphology and bioacoustics reveal five additional species of arboreal microhylids of the genus Anodonthyla from Madagascar. Contributions to Zoology, 79, 1–32.
Vences, M., Glaw, F. & Marquez, R. (eds) (2006) The Calls of the Frogs of Madagascar. 3 Audio CD’s and booklet. Alosa, Barcelona.
Vences, M., Kosuch, J., Glaw, F., Böhme, W. & Veith, M. (2003) Molecular phylogeny of hyperoliid treefrogs: biogeographic origin of Malagasy and Seychellean taxa and re-analysis of familial paraphyly. Journal of Zoological Systematics and Evolutionary Research, 41, 205–215.
Vieites, D.R., Wollenberg, K.C., Andreone, F., Köhler, J., Glaw, F. & Vences, M. (2009) Vast underestimation of Madagascar’s biodiversity evidenced by an integrative amphibian inventory. Proceedings of the National Academy of Sciences of the U.S.A., 106, 8267–8272.
Wohltmann, A., du Preez, L., Rödel, M.O., Köhler, J. & Vences, M. (2007) Endoparasitic mites of the genus Endotrombicula Ewing, 1931 (Acari: Prostigmata: Parasitengona: Trombiculidae) from African and Madagascan anurans, with description of a new species. Folia Parasitologica, 54, 225–235.
Wollenberg, K.C., Vieites, D.R., van der Meijden, A., Glaw, F., Cannatella, D.C. & Vences, M. (2008) Patterns of endemism and species richness in Malagasy cophyline frogs support a key role of mountainous areas for speciation. Evolution, 62, 1890–1907.