Abstract
We describe a new frog species in the genus Rugosa from Sado Island, Japan. This new species, Rugosa susurra, is ge-netically close to R. rugosa Temminck and Schlegel, but is morphologically distinguishable and postzygotically isolatedfrom the latter species. The skin over the entire body is much smoother, and the abdomen and ventral surface of hindlimbare deep yellow whereas these are pale yellow or grayish yellow in R. rugosa. In addition, duration of the advertisementcall of Rugosa susurra is definitely longer than that of R. rugosa. Rugosa susurra is the first endemic amphibian species to Sado Island in Japan.References
Chang, T.K. (1933) Two new amphibian records from Chekiang. Peking Natural History Bulletin, 8, 75–80.
Che, J., Pang, J., Zhao, H., Wu, G., Zhao, E. & Zhang, Y. (2007) Phylogeny of Raninae (Anura: Ranidae) inferred from mitochondrial and nuclear sequences. Molecular Phylogenetics and Evolution, 43, 1–13.
Dubois, A. (1992) Notes sur la classification des Ranidae (Amphibiens, Anoures). Bulletin de la Société d'anthropologie de Lyon, 61, 305–352.
Fei, L., Ye, C. & Huang, Y. (1991 ‘‘1990’’) Key to Chinese amphibians. Publishing house for scientific and technological literature, Chongqing, 364pp. (in Chinese)
Fei, L., Ye, C., Jiang, J., Feng, X. & Huang, Y. (2005) An illustrated key to Chinese amphibians. Sichuan publishing house of science and technology, Chongqing, 340pp. (in Chinese)
Frost, D.R., Grant, T., Faivovich, J., Bazin, R.H., Haas, A., Haddad, C.F.B., de Sa´ , R.O., Channing, A., Wilkinson, M., Donnellan, S.C., Raxworthy, C.J., Campbell, J.A., Blotto, B.L., Moler, P., Drewes, R.C., Nussbaum, R.A., Lynch, J.D., Green, D.M. & Wheeler, W.C. (2006) The amphibian tree of life. Bulletin of the American museum of natural history, 297, 1–370.
Gosner, K.L. (1960) A simplified table for staging anuran embryos and larvae with notes on identification. Herpetologica, 16, 183–190.
Graves, J.A.M. (2008) Weird animal genomes and the evolution of vertebrate sex and sex chromosomes. Annual Review of Genetics, 42, 565–586.
Hasegawa, Y., Ueda, H. & Sumida, M. (1999) Clinal geographic variation in the advertisement call of the wrinkled frog, Rana rugosa. Herpetologica, 55, 318–324.
Kuzmin, S., Matsui, M., Wenge, Z. & Kaneko, Y. (2004) Glandirana emeljanovi. In: IUCN 2011. IUCN Red List of Threatened Species. Version 2011.2. Available from http://www.iucnredlist.org (accessed 30 November 2011)
Machida, H., Matsuda, T., Umetsu, M. & Koizumi, T. (2006) Landscape of Japan (5) Tokyo University Press, Tokyo, 392pp. (in Japanese)
Maeda, N. & Matsui, M. (1999) Frogs and toads of Japan. Revised edition. Bun-Ichi Sogo Shuppan, Tokyo, 223pp. (in Japanese with English abstract)
Matsui, M., Iwasawa, H., Takahashi, H., Hayashi, T. & Kumakura, M. (1992) Invalid specific status of Hynobius sadoensis Sato: Electrophoretic evidence (Amphibia: Caudata). Journal of Herpetology, 26, 308–315.
Miura, I., Ohtani, H., Hanada, H., Ichikawa, Y., Kashiwagi, A. & Nakamura, M. (1997) Evidence for two successive pericentric inversions in sex lampbrush chromosomes of Rana rugosa (Anura: Ranidae). Chromosoma, 106, 178–182.
Miura, I. (2007) An evolutionary witness: the frog Rana rugosa underwent change of heterogametic sex from XY male to ZW female. Sexual Development, 1, 323–331.
Motokawa, M. (2008) Mammalogy in Japan (1). Tokyo University Press, Tokyo, 311pp. (in Japanese)
Myers, C. W. & Duellman, W. E. (1982) A new species of Hyla from Cerro Colorado, and other tree frog records and geographical notes from western Panama. American Museum Novitates, 2752, 1–32.
Nikolsky, A. M. (1918) Fauna of Russia and adjacent countries. Amphibia. Israel program for Scientific Translation, Jerusalem, 225pp. (Original in Russian)
Nishioka, M., Miura, I., & Saitou, K. (1993a) Sex chromosomes of Rana rugosa with special reference to local differences in sex determining mechanism. Scientific Report of the Laboratory for Amphibian Biology, Hiroshima University, 12, 55–81.
Nishioka, M., Kodama, Y., Sumida, M. & Ryuzaki, M. (1993b) Systematic evolution of 40 populations of Rana rugosa distributed in Japan elucidated by electrophoresis. Scientific Report of the Laboratory for Amphibian Biology, Hiroshima University, 12, 83–131.
Nishioka, M., Hanada, H., Miura, I. & Ryuzaki, M. (1994) Four kinds of sex chromosomes in Rana rugosa. Scientific Report of the Laboratory for Amphibian Biology, Hiroshima University, 13, 1–34.
Nishioka, M. & Hanada, H. (1994) Sex of reciprocal hybrids between the Hamakita (XX-XY type) population and the Murakami (ZW-ZZ type) population of Rana rugosa. Scientific Report of the Laboratory for Amphibian Biology, Hiroshima University, 13, 35–50.
Ogata, M., Lee, J.Y., Kim, S., Ohtani, H., Sekiya, K., Igarashi, T., Hasegawa, Y., Ichikawa, Y. & Miura, I. (2002) The prototype of sex chromosomes found in Korean populations of Rana rugosa. Cytogenetic and Genome research, 99, 185–193.
Ogata, M., Ohtani, H., Igarashi, T., Hasegawa, Y., Ichikawa, Y. & Miura, I. (2003) Change of the heterogametic sex from male to female in the frog. Genetics, 164, 613–620.
Ogata, M., Hasegawa, Y., Ohtani, H., Mineyama,M. & Miura, I. (2008) The ZZ/ZW sex-determining mechanism originated twice and independently during evolution of the frog, Rana rugosa. Heredity, 100, 92–99.
Ohshima, K. (1990) The history of straits around the Japanese Islands in the late-Quaternary. The Quaternary Research (Daiyonki Kenkyu), 29, 193–208. (in Japanese with English abstract)
Ohtani, H., Sekiya, K., Ogata, M. & Miura, I. (2012) The postzygotic isolation of a unique morphotype of frog Rana rugosa (Ranidae) found on Sado Island, Japan. Journal of Herpetology, 46, 325–330.
Paul, B. (2001) Praat, a system for doing phonetics by computer. Glot International 5:9/10, 341–345.
R Development Core Team (2010) R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. Available from http://www.R-project.org.. (accessed 20 September 2010)
Sato, I. (1940) On a Hynobius from Sado Island. Bulletin of the Biogeographical Society of Japan, 10, 163–170.
Sekiya, K., Ohtani, H., Ogata, M. & Miura, I. (2010) Phyletic diversity in the frog Rana rugosa (Anura: Ranidae) with special reference to a unique morphotype found from Sado Island, Japan. Current Herpetology, 29, 69–78.
Shinohara, A., Campbell K.L. & Suzuki, H. (2005) An evolutionary view on the Japanese talpids based on nucleotide sequences. Mammal Study, 30, S19–S24.
Stejneger, L. (1907) Herpetology of Japan and adjacent territory. Bulletin of United States National Museum, 58, 1–577.
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., & Kumar, S. (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28, 2731–2739.
Ting, H.P. & T’sai, M.C. (1979) A new species of frog (Rana minimus) from Fujian Province. Acta Zootaxonomica Sinica, 4, 297–300. (in Chinese with English abstract)
Ueda, H., Hasegawa Y., & Marunouchi, J. (1998) Geographical differentiation in a Japanese stream-breeding frog, Buergeria buergeri, elucidated by morphometric analyses and crossing experiments. Zoological Science, 15, 615–622.
Wiens, J.J., Sukumaran, J., Pyron, R.A., & Brown, R.M. (2009) Evolutionary and biogeographic origins of high tropical diversity in old world frogs (Ranidae). Evolution, 63, 1217–1231.