Abstract
Male, female and immature stages of Phyllocnistis tethys Moreira & Vargas sp. nov. (Lepidoptera; Gracillariidae) from the Atlantic Rain Forest, coastal mountains of southern Brazil, are described and illustrated, using both optical and scanning electron microscopy. A preliminary analysis of mitochondrial (COI) DNA sequences including putative members of congeneric species is also provided. The immature stages are associated with the passion vine Passiflora organensis (Passifloraceae). The hypermetamorphic, endophyllous larva has four instars; the first, second and third instars are sap-feeders, associated primarily with the spongy parenchyma, and construct a blotch mine in the lower surface of the lamina; the fourth, non-feeding (spinning) instar constructs a flimsy endophyllous cocoon at the end of the mine, where pupation occurs. This is the first species of Phyllocnistis Zeller described from Brazil, and the first leaf-mining gracillariid associated with Passifloraceae.
References
Benson, W.W., Brown Jr., K.S. & Gilbert, L.E. (1975) Coevolution of plants and herbivores: passion flower butterflies. Evolution, 29, 659–680.
Brown Jr., K.S. (1981) The biology of Heliconius and related genera. Annual Review of Entomology, 26, 427–457.
Davis, D.R. (1987) Gracillariidae. In: Stehr, F.W. (Ed.). Immature Insects, Vol. I. Kendall/Hunt Publishing Company, Dubuque, pp. 372–374.
Davis, D.R. (1994) New leaf-mining moths from Chile, with remarks on the history and composition of Phyllocnistinae (Lepidoptera: Gracillariidae). Tropical Lepidoptera, 5, 65–75.
Davis, D.R. & Miller, S.E. (1984) Gracilliidae. In: Heppner, J. B. (Ed.). Atlas of Neotropical Lepidoptera, Checklist: Part 1. Dr. W. Junk Publishers, The Hague, pp. 25–27.
Davis, D.R. & Wagner, D.L. (2011) Biology and systematics of the New World Phyllocnistis Zeller leafminers of the avocado genus Persea (Lepidoptera, Gracillariidae). ZooKeys, 97, 39–73.
De Prins, J. & De Prins, W. (2012) Global Taxonomic Database of Gracillariidae (Lepidoptera). World Wide Web electronic publication (http: //www.gracillariidae.net) [accessed on June 14, 2012]
De Prins, J. & Kawahara, A.Y. (2009) On the taxonomic history of Phyllocnistis Zeller, 1848 (Gracillariidae). Nota Lepidopterologica, 32, 113–121.
Doyle, J.J. & Doyle, J.L. (1987) A rapid isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 9, 11–15.
Engler-Chaouat, H.S. & Gilbert, L.E. (2007) De novo synthesis vs. sequestration: negatively correlated metabolic traits and the evolution of host plant specialization in cyanogenic butterflies. Journal of Chemical Ecology, 33, 25–42.
Felsenstein, J. (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution, 39, 783–791.
Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299.
Gilbert, L.E. (1991) Biodiversity of a Central American Heliconius community: pattern, process, and problems. In Price, P.W, Lewinsohn, T.M., Fernandez, G.W. & Benson, W.W. (Eds.). Plant-Animal Interactions. Evolutionary Ecology in Tropical and Temperate Regions. John Wiley & Sons, New York, pp. 403–427.
Guindon, S., Dufayard, J.F., Lefort, V., Anisimova, M., Hordijk, W. & Gascuel O. (2010) New algorithms and methods to estimate Maximum-Likelihood phylogenies: assessing the performance of PhyML 3.0. Systematic Biology, 59, 307–21.
Hering, E.M. (1958) Neue microlepidopteren von Tucuman. Acta Zoologica Lilloana, 15, 303–312.
Kawahara, A.Y., Nishida, K. & Davis, D.R. (2009) Systematics, host plants, and life histories of three new Phyllocnistis species from the central highlands of Costa Rica (Lepidoptera, Gracillariidae, Phyllocnistinae). ZooKeys, 27, 7–30.
Kawahara, A.Y., Ohshima, I., Kawakita, A., Regier, J.C., Mitter, C., Cummings, M.P., Davis, D.R., Wagner, D.L., De Prins, J. & Lopez-Vaamonde, C. (2011) Increased gene sampling strengthens support for higher-level groups within leaf-mining moths and relatives (Lepidoptera: Gracillariidae). BMC Evolutionary Biology, 11, 182.
Labandeira, C.C., Dilcher, D.L., Davis, D.R. & Wagner, D.L. (1994) Ninety-seven million years of angiosperm insect association: Paleobiological insights into the meaning of coevolution. Proceedings of the National Academy of Sciences of the U.S.A., 91, 12278–12282.
Mondin, C.A., Cervi, C. & Moreira, G.R.P. (2011) Sinopse das espécies de Passiflora L. (Passifloraceae) do Rio Grande do Sul, Brasil. Brazilian Journal of Biosciences, 9, s.1, 3–27.
Moreira, G.R.P., Ferrari, A., Mondin, C.A. & Cervi, C. (2011) Panbiogeographical analysis of passion vines at their southern limit of distribution in the Neotropics. Brazilian Journal of Biosciences, 9, s.1, 28–40.
Nahrstedt, A. & Davis, R.H. (1983) Occurrence, variation and biosynthesis of the cyanogenic glucosides linamarin and lotaustralin in species of the Heliconiini (Insecta: Lepidoptera). Comparative Biochemistry and Physiology Part B, 75, 65–73.
Nieukerken, E.J., Kaila, L., Kitching, I.J., Kristensen, N.P., Lees, D.C., Minet, J., Mitter, C., Mutanen, M., Regier, J.C, Simonsen, T.J., et al. (2011) Order Lepidoptera Linnaeus, 1758. In Zhang, Z.Q. (Ed.) Animal Biodiversity: An Outline of Higher-level Classification and Survey of Taxonomic Richness. Zootaxa, 3148, 212–221.
Posada, D. (2008) jModelTest: Phylogenetic model averaging. Molecular Biology and Evolution, 25, 1253–1256.
Rodríguez, F., Oliver, J.L., Marin, A. & Medina, J.R. (1990) The general stochastic model of nucleotide substitution. Journal of Theoretical Biology, 142, 485–501.
Sohn, J.-C., Labandeira, C.C., Davis, D. & Mitter, C. (2012) An annotated catalog of fossil and subfossil Lepidoptera (Insecta: Holometabola) of the world. Zootaxa, 3286, 1–132.
Spencer, K.C. (1988) Chemical mediation of coevolution in the Passiflora-Heliconius interaction. In Spencer, K.C. (Ed.) Chemical Mediation of Coevolution. Academic Press, New York, pp. 167–240.