Abstract
Three new species of Trischistoma and two new species of Tripylina (Trischistomatidae) are described. Trischistoma ripariana n. sp. was collected in the surface organic material and upper rhizosphere soil on a stream bank in Oakville, California, USA. It is characterized by a short, thin body, the vulva at 79–83%, a small index c (17–29) and a short tail, 34–57 µm. Trischistoma corticulensis n. sp. was found in moss on tree bark in a tropical forest at the La Mancha Ecological Institute, Veracruz State, México. The vulva is at 67–73% and the tail is elongate conoid (51–84 µm). Trischistoma helicoformis n. sp. was collected on lichen growing on tree bark near the Carretera Interamericana in Costa Rica. It is characterized by its spiral shape after fixation, the tail length (76–101 µm), a very small index c (10–14.5) and very small sclerotised pieces around the vagina. Tripylina rorkabanarum n. sp. was collected from moss on tree bark in a tropical forest at the La Mancha Ecological Institute. It is characterized by the presence of two cervical setae, the position of the subventral teeth posterior to the dorsal tooth, the absence of sclerotized pieces around the vagina and the distance of the dorsal tooth from the anterior, 10–15 μm. Tripylina iandrassyi n. sp. was collected from soil around a banana tree at the La Mancha Ecological Institute. It is characterized by the presence of a post-uterine sac, well-developed buccal lips, subventral teeth located posterior to the dorsal tooth, one cervical seta in females and two in males, and by spicules not completely surrounded by a muscular sheath.
References
Andrássy, I. (1967) Ergebnisse der zoologischen Forschungen von Dr. Z. Kaszab in der Mongolei. 92. Weitere Bodennematodenaus der Jahren 1964 und 1965. Opuscula Zoologica (Budapest), 6, 203–233.
Andrássy, I. (1985) A dozen new nematode species from Hungary. Opuscula Zoologica (Budapest), 19–29, 3–39.
Andrássy, I. (2006) Three new species of the family Tripylidae (Penetrantia: Enoplida) from South America. International Journal of Nematology, 16, 208–216.
Andrássy, I. (2007) Free-living nematodes of Hungary (Nematoda, Errantia), II. Hungarian Natural History Museum, Budapest, 496 pp.
http://dx.doi.org/10.1163/156854108783476296Andrássy, I. (2011) Two unusually slender nematode species of Trischistoma Cobb, 1913 (Enoplida: Tripylidae). Nematology, 13, 561–567.
http://dx.doi.org/10.1163/138855410x533635Anya, A.O. (1976) Studies on the reproductive physiology of nematodes: the phenomenon of sexual attraction and the origin of attractants in Aspiculuris tetraptera. International Journal of Parasitology, 6, 173–177.
http://dx.doi.org/10.1016/0020-7519(76)90076-xArchidona-Yuste, A., Navas-Cortes, J.A., Cantalapiedra-Navarrete, C., Palomares-Rius, J.E. & Castillo, P. (2015) Cryptic diversity and species delimitation in the Xiphinema americanum-group complex (Nematoda: Longidoridae) as inferred from morphometrics and molecular markers. Zoological Journal of the Linnean Society, 2015, 1–35.
http://dx.doi.org/10.1111/zoj.12316Argo, A.D. & Heyns, J. (1973) New and little known species of the nematode families Monhysteridae and Tripylidae from South Africa. Phytophylactica, 5, 149–154.
Asghari R., Pourjam, E., Heydari, R., Zhao, Z.Q. & Ramaji, F.A.(2012) Tripylina gorganensis n. sp. (Triplonchida: Tripylidae) from northern Iran. Nematology, 14, 613–621.
http://dx.doi.org/10.1163/156854112x624609Bastian, H.C. (1865) Monograph of the Anguillulidae, or free nematoids, marine, land, and freshwater, with descriptions of 100 new species. Transactions of the Linnean Society of London, 25, 73–184.
http://dx.doi.org/10.1111/j.1096-3642.1865.tb00179.xBonner, T.P. & Etges, F.T. (1967) Chemically-mediated sexual attraction in Trichinella spiralis. Experimental Parasitology, 21, 53–60.
http://dx.doi.org/10.1016/0014-4894(67)90067-7Brzeski, M.W. & Winiszewska-Ślipińska, G. (1993) Taxonomy of Tripylidae (Nematoda: Enoplia). Nematologica, 39, 12–52.
http://dx.doi.org/10.1163/187529293x00024Brzeski, M.W. (1963) Nematode genera of the family Tripylidae (Nematoda, Enoplida). Acta Zoological Cracoviensia, 8, 295–308.
Cid del Prado-Vera, I. & Subbotin, S.A. (2012) Belonolaimus maluceroi sp. n. (Tylenchida: Belonolaimidae) from a tropical forest in México and key to the species of Belonolaimus. Nematropica, 42, 201–210.
Cid del Prado-Vera, I., Ferris, H. & Nadler, S.A. (2010) Soil inhabiting nematodes of the genera Trischistoma, Tripylina and Tripyla from México and the USA with descriptions of new species. Journal of Nematode Morphology and Systematics, 13, 29–49.
Cid del Prado-Vera, I., Ferris, H., Nadler, S.A. & Lamothe-Argumedo, R. (2012) Four new species of Tripylina Brzeski, 1963 (Enoplida: Tripylidae) from México, with an emended diagnosis of the genus. Journal of Nematode Morphology and Systematics, 15, 71–86.
Cobb, N.A. (1913) New nematode genera found inhabiting fresh water and non-brackish soils. Journal of Washington Academy of Sciences, 3, 432–445.
http://dx.doi.org/10.5962/bhl.part.20323Coomans, A. & Lima, M.B. (1965) Description of Anatonchus amiciae n. sp. (Nematoda, Mononchidae) with observations on its juvenile stages and anatomy. Nematologica, 11, 413–431.
http://dx.doi.org/10.1163/187529265x00348De Ley, P. & Blaxter, M. (2004) A new system for Nematoda, combining morphological characters with molecular trees, and translating clades into ranks and taxa. Nematology Monographs and Perspectives, 2, 633–653.
De Maeseneer, J. & d’Herde, J. (1963) Méthodes utilisées pour l’étude des anguillules libres du sol. Revue d’Agriculture, 16, 441–447.
De Man, J.G. (1876) Onderzooekingen over vrij in de aarde levende nematoden. Tijdschrift der Nederlandische dierkundige Vereeniging, 2, 1–119.
De Man, J.G. (1880) Die einheimischen, frei in der reinen Erde und im sssen Wasser lebenden Nematoden. Tijdschrift der Nederlandische Dierkundige Vereeniging, 5, 1–104.
Goater, T.M., Goater, C.P. & Esch, G.W. (2013) Parasitism: The Diversity and Ecology of Animal Parasites. Cambridge University Press, Cambridge, 524 pp.
Gutiérrez-Gutiérrez, C., Cantalapiedra-Navarrete, C., Remesal, E., Palomares-Rius, J.E., Navas-Cortés, J.A. & Castillo, P. (2013) New insight into the identification and molecular phylogeny of dagger nematodes of the genus Xiphinema (Nematoda: Longidoridae) with description of two new species. Zoological Journal of the Linnean Society, 169, 548–579.
http://dx.doi.org/10.1111/zoj.12071
Holterman, M. & Holovachov, O. (2007) Phylogeny and biogeography of Triplonchida. Phytopathology, 97 (Supplement), 132.
Holterman, M., van der Wurff, A., van den Elsen, S., van Megen, H., Bongers, T., Holovachov, O., Bakker, J. & Helder, J. (2006) Phylum-wide analysis of SSU rDNA reveals deep phylogenetic relationships among nematodes and accelerated evolution toward crown clades. Molecular Biology and Evolution, 23, 1792–1800.
http://dx.doi.org/10.1093/molbev/msl044Jenkins, W.R. (1964) A rapid centrifugal-flotation technique for separating nematodes from soil. Plant Disease Reporter, 48, 69.
Lints, R. & Hall, D.H. (2009) Male alimentary system, proctodeum. In: Herndon, L.A. (Ed.), WormAtlas. Last revision: July 24, 2013. Available from: http://www.wormatlas.org/male/alimproct/Procframeset.html (accessed 23 March 2015)
http://dx.doi.org/10.3908/wormatlas.2.2
Loytynoja, A. & Milinkovitch, M.C. (2003) A hidden Markov model for progressive multiple alignment. Bioinformatics, 19, 1505–1513.
http://dx.doi.org/10.1093/bioinformatics/btg193Meldal, B.H.M., Debenham, N.J., De Ley, P., De Ley I.T., Vanfleteren, J.R., Vierstraete, A.R., Bert, W., Borgonie, G., Moens, T., Tyler, P.A., Austen, M.C., Blaxter, M.L. Rogers, A.D. & Lambshead, P.J.D. (2007) An improved molecular phylogeny of the Nematoda with special emphasis on marine taxa. Molecular Phylogenetics and Evolution, 42, 622–636.
http://dx.doi.org/10.1016/j.ympev.2006.08.025Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the Gateway Computing Environments Workshop (GCE), 14 November 2010, New Orleans, LA, pp. 1–8.
Nylander, J.A.A. (2004) MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University.
Pham, T.H., Wang, H.T., Zhao, Z.Q. & Zheng, J.W. (2013) A new species of the genus Tripylina Brzeski, 1963 (Nematoda: Enoplida: Trischistomatidae) from Zhejiang Province, eastern China. Zootaxa, 3717 (2), 158–168.
http://dx.doi.org/10.11646/zootaxa.3717.2.2Ronquist, F. & Huelsenbeck, J. P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.
http://dx.doi.org/10.1093/bioinformatics/btg180Schuurmans Stekhoven, J.H. (1951) Nematodes saprozoaires et libres du Congo Belge. Mémoires de l’Institut Royal des Sciences Naturelles de Belgique, Série 2, 39, 1–79.
Seinhorst, J.W. (1959) A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica, 4, 67–69.
http://dx.doi.org/10.1163/187529259x00381Tahseen, Q. & Nusrat, T. (2010) Some new and known species of the genera Tripylina Brzeski and Trischistoma Cobb 1913 (Nematoda) with a discussion on their relationships. Journal of Nematology, 42,128–138.
Traunspurger, W. (2000) The biology and ecology of lotic nematodes. Freshwater Biology, 44, 29–45.
http://dx.doi.org/10.1046/j.1365-2427.2000.00585.xTraunspurger, W. (2002) Nematoda. In: Rundle, S.D., Robertson, A.L. & Schmid-Araya, J.M. (Eds.), Freshwater Meiofauna, Biology and Ecology. Backhuys Publishers, Leiden, pp. 63–104.
Tsalolikhin, S.Y. (1983) Nematodes of the Families Tobrilidae and Tripylidae of the World Fauna. No.138. Nauka, Leningrad, Russia, 232 pp.
Van Megen, H., van den Elsen, S., Holterman, M., Karssen, G., Mooyman, P., Bongers, T., Holovachov, O., Bakker, J. & Helder, J. (2009) A phylogenetic tree of nematodes based on about 1200 full-length, Nematology, 11, 927–950.
Vinciguerra, M.T. & La Fauci, G. (1978) Nematodi muscicoli dell’isola di Lampedusa. Animalia, 5, 13–37.
Xu, Y.M., Zhao, Z.Q., Wang, J.M. & Zheng, J.W. (2013) A new species of the genus Tripylina Brzeski, 1963 (Nematoda: Enoplida: Trischistomatidae) from Shanxi province, China. Zootaxa, 3630 (3), 561–570.
http://dx.doi.org/10.11646/zootaxa.3630.3.10Yeates, G.W., Bongers, T., de Goede, R.G.M., Freckman, D.W. & Georgieva, S.S. (1993) Feeding habits in soil nematode families and genera - an outline for soil ecologists. Journal of Nematology, 25, 315–331.
Zhao, Z.Q. (2009) A review of the genus Tripylina Brzeski, 1963 (Nematoda: Triplonchida), with descriptions of five new species from New Zealand. Zootaxa, 2238, 1–24.
Zhao, Z.Q. (2011) A review of the genus Trischistoma Cobb, 1913 (Nematoda: Enoplida), with descriptions of four new species from New Zealand. Zootaxa, 3045, 1–25.
Zhao, Z.Q. (2012) Analysis of primary structure loops from Hairpins 35 and 48 of the Nematoda SSU rRNA gene provides further evidence that the genera Tripylina Brzeski, 1963, Trischistoma Cobb, 1913 and Rhabdolaimus de Man, 1880 are members of Enoplida. Zootaxa, 3208, 41–57.
Zhao, Z.Q. & Buckley, T.R. (2009) Phylogenetic analysis of nematode nuclear 18S rDNA sequences indicates the genus Tripylina Brzeski, 1963 (Nematoda: Tripylidae de Man, 1876) should be placed in Enoplida. Zootaxa, 2238, 24–32.
Zullini, A. (2006) Order Triplonchida. In: Abebe, E., Andrássy, I. & Traunspurger, W. (Eds.), Freshwater Nematodes, Ecology and Taxonomy. CABI Publishing, Wallingford, pp. 293–323.
http://dx.doi.org/10.1079/9780851990095.0293