Abstract
The presence of the Afro-Australian genus Conochironomus Freeman, 1961 (Diptera: Chironomidae) in Asia has been recognised only informally. An unpublished thesis included Conochironomus from Singapore, and the genus has been keyed from Malaysia without named species. Here, the Sumatran Conochironomus tobaterdecimus (Kikuchi & Sasa, 1980) comb. n. is recorded from Singapore and Thailand. The species is transferred from Sumatendipes Kikuchi & Sasa, 1980, rendering the latter a junior synonym (syn. n.) of Conochironomus Freeman. Conochironomus nuengthai sp. n. and Conochironomus sawngthai sp. n. are described as new to science, based on adult males from Chiang Mai, Thailand. All species conform to existing generic diagnoses for all life stages, with features from male and female genitalia, pupal cephalic tubercles and posterolateral ‘spurs’ of tergite VIII providing evidence for species distinction. Some larvae are linked to C. tobaterdecimus through molecular barcoding. Variation in other larvae, which clearly belong to Conochironomus and are common throughout Thailand, means that they cannot be segregated to species. Larval habitats include pools in river beds, urban storage reservoirs, drains with moderately high nutrient loadings, and peat swamps. Endochironomus effusus Dutta, 1994 from north-eastern India may be a congener but may differ in adult morphology, thereby precluding formal new combination until discrepancies can be reconciled. Many problems with vouchering taxonomic and molecular material are identified that need to be rectified in the future.
References
Brundin, L. (1966) Transantarctic relationships and their significance, as evidenced by chironomid midges with a monograph of the subfamilies Podonominae and Aphroteniinae and the austral Heptagyiae. Kungliga Svenska Vetenskapsakademiens Handlingar, 11, 1–472.
Cranston, P.S. (1995) Morphology. In: Armitage, P.D., Cranston, P.S. & Pinder, L.C.V. (Eds.), The Chironomidae. Biology and ecology of non-biting midges. Chapman & Hall, London, pp. 11–30.
http://dx.doi.org/10.1007/978-94-011-0715-0_2Cranston, P.S. (1996) Identification Guide to the Chironomidae of New South Wales. AWT Identification Guide 1, Australian Water Technologies Pty Ltd, Sydney, 376 pp.
Cranston, P.S. (2000) Electronic Guide to The Chironomidae of Australia. Available from: http://apes.skullisland.info/sites/default/files/webfiles/members/pete/start.pdf (accessed 9 March 2016)
Cranston, P.S. (2004) Insecta: Diptera, Chironomidae. In: Yule, C.M. & Yong, H.S. (Eds.), The Freshwater Invertebrates of Malaysia and Singapore. Academy of Sciences, Kuala Lumpur, pp. 710–734.
Cranston, P.S. (2007) The Chironomidae larvae associated with the tsunami-impacted waterbodies of the coastal plain of south-western Thailand. Bulletin of the Raffles Museum, 55, 231–244.
Cranston, P.S. (2015) Evidence from molecules and morphology expands Podonomopsis Brundin (Diptera: Chironomidae: Podonominae) to include ‘genus Chile’. Invertebrate Systematics, 29, 610–627.
http://dx.doi.org/10.1071/IS15018Cranston, P.S., Ang, Y.C., Heyzer, A., Lim, R.B.H., Wong, W.H., Woodford, J.M. & Meier, R. (2013) The nuisance midges (Diptera: Chironomidae) of Singapore’s Pandan and Bedok reservoirs. The Raffles Bulletin of Zoology, 61, 779–793.
Cranston, P.S. & Bickel, D. (2015) Confirmatory bias and photographing the difference. Fly Times, 55, 21–23.
Cranston, P.S., Dillon, M., Pinder, L.C.V. & Reiss, F.R. (1989) Keys and diagnoses of the adult males of the subfamily Chironominae (Diptera, Chironomidae). Entomologica scandinavica Supplement, 34, 353–502.
Cranston, P.S., Hardy, N.B. & Morse, G.E. (2012) A dated molecular phylogeny for the Chironomidae (Diptera). Systematic Entomology, 37, 172–188.
http://dx.doi.org/10.1111/j.1365-3113.2011.00603.x
Cranston, P.S. & Hare, L. (1995) Conochironomus Freeman: an Afro-Australian Chironomini genus revised (Diptera: Chironomidae). Systematic Entomology, 20, 247–264.
http://dx.doi.org/10.1111/j.1365-3113.1995.tb00096.xCranston, P.S., Martin, J. & Spies, M. (2016) Cryptic species in the nuisance midge Polypedilum nubifer (Skuse) (Diptera: Chironomidae) and the status of Tripedilum Kieffer. Zootaxa, 4079 (4), 429–447.
http://dx.doi.org/10.11646/zootaxa.4079.4.3Dutta, T.K., Majumdar, A. & Chaudhuri, P.K. (1994) On two new species of the genus Endochironomus KIEFFER (Diptera: Chironomidae) from the Himalaya, West Bengal, India. Studia dipterologica, 1, 253–258.
Ekrem, T., Willassen, E. & Stur, E. (2007) A comprehensive DNA sequence library is essential for identification with DNA barcodes. Molecular Phylogenetics and Evolution, 43, 530–542.
http://dx.doi.org/10.1016/j.ympev.2006.11.021Epler, J., Ekrem, T. & Cranston, P.S. (2013) The larvae of Chironominae (Diptera: Chironomidae) of the Holarctic region — Keys and diagnoses. Insect Systematics and Evolution Supplement, 66, 387–556.
Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294–299.
Freeman, P. (1955) Contributions a I’étude de la faune entomologique du Ruanda-Urundi (Mission P. Basilewsky 1953). XXIX. Diptera Chironomidae. Annales du Musée Royale du Congo Belge, 36, 287–289.
Freeman, P. (1961) The Chironomidae (Diptera) of Australia. Australian Journal of Zoology, 9, 611–737.
http://dx.doi.org/10.1071/ZO9610611ICZN International Commission on Zoological Nomenclature (1999) International Code of Zoological Nomenclature. 4th Edition. The International Trust for Zoological Nomenclature, London, 306 pp.
Karunakaran, S. (1969) Studies on the Bionomics and Taxonomy of Singapore Chironomidae. PhD Thesis. Department of Zoology, University of Singapore, Singapore, iv + 404 pp.
Kikuchi, M. & Sasa, M. (1990) Studies on the chironomid midges (Diptera, Chironomidae) of the Lake Toba area, Sumatra, Indonesia. Japanese Journal of Sanitary Zoology, 41, 291–329.
Kranzfelder, P., Anderson, A.M., Egan, A.T., Mazack, J.E., Bouchard, R.W. Jr., Rufer, M.M. & Ferrington, L.C.Jr. (2015) Use of Chironomidae (Diptera) surface-floating pupal exuviae as a rapid bioassessment protocol for water bodies. Journal of Visualized Experiments, 101, e52558.
http://dx.doi.org/10.3791/52558Kranzfelder, P., Ekrem, T. & Stur, E. (2016) Trace DNA from insect skins: a comparison of five extraction protocols and direct PCR on chironomid pupal exuviae. Molecular Ecology Resources, 16, 353–363.
http://dx.doi.org/10.1111/1755-0998.12446Krosch, M.N. & Cranston, P.S. (2012) Non-destructive DNA extraction from Chironomidae, including fragile pupal exuviae, extends analyzable collections and enhances vouchering. Chironomus. Newsletter on Chironomidae Research, 25, 22–27.
Krosch, M.N., Cranston, P.S., Baker, A.M. & Vink, S. (2015) Molecular data extend Australian Cricotopus midge (Chironomidae) species diversity, and provide a phylogenetic hypothesis for biogeography and freshwater monitoring. Zoological Journal of the Linnean Society, 175, 496–509.
http://dx.doi.org/10.1111/zoj.12284Lin, X., Stur, E. & Ekrem, T. (2015) Exploring genetic divergence in a species-rich insect genus using 2790 DNA Barcodes. PLoS ONE, 10 (9), e0138993.
http://dx.doi.org/10.1371/journal.pone.0138993
Meier, R., Wong, W., Srivathsan, A. & Foo, M. (2015) $1 DNA barcodes for reconstructing complex phenomes and finding rare species in specimen-rich samples. Cladistics, 32, 100–110.
http://dx.doi.org/10.1111/cla.12115Min, J.L. (2014) Studies of tropical lacustrine Chironomidae (Insecta: Diptera) in Malaysia. Unpubl. BSc Hons. Thesis, Monash University Malaysia, Kuala Lumpur. [unkown pagination]
Pinho, L.S., Mendes, H.F. & Andersen, T. (2013) Revision of Beardius Reiss et Sublette, 1985 (Diptera: Chironomidae), with the description of twenty new species. Zootaxa, 3742 (1), 1–78.
http://dx.doi.org/10.11646/zootaxa.3742.1.1Pramual, P., Simwisat, K. & Martin, J. (2016) Identification and reassessment of the specific status of some tropical freshwater midges (Diptera: Chironomidae) using DNA barcode data. Zootaxa, 4072 (1), 39–60.
http://dx.doi.org/10.11646/zootaxa.4072.1.2Raunio, J., Paavola, R. & Muotka, T. (2007) Effects of emergence phenology, taxa tolerances and taxonomic resolution on the use of the chironomid pupal exuvial technique in river biomonitoring. Freshwater Biology, 52, 165–176.
http://dx.doi.org/10.1111/j.1365-2427.2006.01670.xSæther, O.A. (1977) Female genitalia in Chironomidae and other Nematocera: morphology, phylogenies, keys. Bulletin of the Fisheries Research Board of Canada, 197, i–viii + 1–209.
Sæther, O.A. (1980) A glossary of chironomid morphology terminology (Diptera: Chironomidae). Entomologica scandinavica Supplement, 14, 1–51.
Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H. & Flook, P. (1994) Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Annals of the Entomological Society of America, 87, 651–701.
http://dx.doi.org/10.1093/aesa/87.6.651Sriariyanuwath, E., Sangpradub, N. & Hanjavanit, C. (2015) Diversity of chironomid larvae in relation to water quality in the Phong River, Thailand. AACL Bioflux, 8, 933–945.
Wong, W.H., Tay, Y.C, Puniamoorthy, J., Balke, M., Cranston, P.S. & Meier, R. (2014) ‘Direct PCR’ optimization yields a rapid, cost-effective, nondestructive and efficient method for obtaining DNA barcodes without DNA extraction. Molecular Ecology Resources, 14, 1271–1280.
http://dx.doi.org/10.1111/1755-0998.12275Yuchen, A., Wong, L.J. & Meier, R. (2013) Using seemingly unnecessary illustrations to improve the diagnostic usefulness of descriptions in taxonomy—a case study on Perochaeta orientalis (Diptera, Sepsidae). ZooKeys, 355, 9–27.
http://dx.doi.org/10.3897/zookeys.355.6013