Abstract
We present evidence from the standard cytochrome c oxidase subunit I (COI) barcoding marker and from new collections, showing that the males and females of C. ecotono Werenkraut & Ramírez were mismatched, and describe the female of that species for the first time. An undescribed male from Chile is assigned to the new species Coptoprepes laudani, together with the female that was previously thought as C. ecotono. The matching of sexes is justified after a dual cladistics analysis of morphological and sequence data in combination. New locality data and barcoding sequences are provided for other species of Coptoprepes, all endemic of the temperate forests of Chile and adjacent Argentina. Although morphology and sequences are not conclusive on the relationships of Coptoprepes species, the sequence data suggests that the species without a retrolateral tibial apophysis may belong to an independent lineage.
References
Aisen, S. & Ramírez, M.J. (2015) A revision and phylogenetic analysis of the spider genus Oxysoma Nicolet (Araneae: Anyphaenidae, Amaurobioidinae). Zootaxa, 3997 (1), 1–61.
http://dx.doi.org/10.11646/zootaxa.3997.1.1Barrett, R.D.H. & Hebert, P.D.N. (2005) Identifying spiders through DNA barcodes. Canadian Journal of Zoology, 83, 481–491.
http://dx.doi.org/10.1139/z05-024Blagoev, G.A., Nikolova, N.I., Sobel, C.N., Hebert, P.D.N. & Adamowicz, S.J. (2013) Spiders (Araneae) of Churchill, Manitoba: DNA barcodes and morphology reveal high species diversity and new Canadian records. BMC Ecology, 13 (44), 1–17.
http://dx.doi.org/10.1186/1472-6785-13-44Castalanelli, M.A., Teale, R., Rix, M.G., Kennington, W.J. & Harvey, M.S. (2014) Barcoding of mygalomorph spiders (Araneae: Mygalomorphae) in the Pilbara bioregion of Western Australia reveals a highly diverse biota. Invertebrate Systematics, 28, 375–385.
Edwards G.B. (2013) A philosophy and methodology for matching opposite sexes of one species, exemplified by a new synonym in Myrmarachne (Araneae: Salticidae). Peckhamia, 111, 1–12.
Goloboff, P.A., Farris, J.S., Källersjö, M., Oxelman, B., Ramírez, M.J. & Szumik, C.A. (2003) Improvements to resampling measures of group support. Cladistics, 19, 324–332.
http://dx.doi.org/10.1111/j.1096-0031.2003.tb00376.xGoloboff, P.A., Farris, J.S. & Nixon, K. (2008) TNT, a free program for phylogenetic analysis. Cladistics, 24, 774–786.
http://dx.doi.org/10.1111/j.1096-0031.2008.00217.xHamilton, C.A., Hendrixson, B.E., Brewer, M.S. & Bond, J.E. (2013) An evaluation of sampling effects on multiple DNA barcoding methods leads to an integrative approach for delimiting species: a case study of the North American tarantula genus Aphonopelma (Araneae, Mygalomorphae, Theraphosidae). Molecular Phylogenetics and Evolution, 71, 79–93.
http://dx.doi.org/10.1016/j.ympev.2013.11.007Labarque, F.M., Soto, E.M., Ramírez, M.J. & Arnedo, M.A. (2015) Chasing ghosts: the phylogeny of Amaurobioidinae ghost spiders (Araneae, Anyphaenidae). Zoologica Scripta, 44, 550–561.
http://dx.doi.org/10.1111/zsc.12119Lanfear, R., Calcott, B., Ho, S.Y.W. & Guindon, S. (2012) PartitionFinder: combined selection of partitioning schemes and substitution models for phylogenetic analyses. Molecular Biology and Evolution, 29, 1695–1701.
http://dx.doi.org/10.1093/molbev/mss020Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetics trees. In: Proceedings of The Gateway Computing Environments Workshop (GCE), 14 November 2010, New Orleans, LA, pp. 1–8.
http://dx.doi.org/10.1109/GCE.2010.5676129Platnick, N.I. & Shadab, M.U. (1978) A review of the spider genus Anapis (Araneae: Anapidae) with a dual cladistic analysis. American Museum Novitates, 2663, 1–23.
Ramírez, M.J. (2003) The spider subfamily Amaurobioidinae (Araneae, Anyphaenidae): a phylogenetic revision at the generic level. Bulletin of the American Museum of Natural History, 277, 1–262.
http://dx.doi.org/10.1206/0003-0090(2003)277%3C0001:TSSAAA%3E2.0.CO;2Ratnasingham, S. & Hebert, P.D.N. (2007) BOLD: The Barcode of Life Data System (www.barcodinglife.org). Molecular Ecology Notes, 7, 355–364.
http://dx.doi.org/10.1111/j.1471-8286.2007.01678.xRobinson, E.A., Blagoev, G. A., Hebert, P.D.N. & Adamowicz, S.J. (2009) Prospects for using DNA barcoding to identify spiders in species-rich genera. ZooKeys, 16, 27–46.
http://dx.doi.org/10.3897/zookeys.16.239Ronquist, F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.
http://dx.doi.org/10.1093/bioinformatics/btg180Rubio, G.D. & Ramírez, M.J. (2015) Taxonomic revision of the American spider genus Arachosia (Araneae: Anyphaenidae). Zootaxa, 3932 (1), 1–105.
http://dx.doi.org/10.11646/zootaxa.3932.1.1Werenkraut, V. & Ramírez, M.J. (2009) A revision and phylogenetic analysis of the spider genus Coptoprepes Simon (Araneae: Anyphaenidae, Amaurobioidinae). Zootaxa, 2212, 1–40.