Abstract
A new perlid stonefly, Phanoperla constanspina sp. nov. is described from Mt. Malindang, northern Mindanao, Philippines. The male of the new species is distinguished by lacking lobes on penial sac and large black spines at the penial apex. The female is distinguished by the egg. DNA barcoding was used to associate male, female, and nymphal specimens with 0% divergence. Morphological variation was observed in the shape of the hemitergal anterior processes and the 9th tergal setal patches of male adult and body pigmentation of the nymph. A key to the Philippine Phanoperla species and a checklist of Oriental Phanoperla are also provided.
References
Alexander, L.C., Delion, M.D. Hawthorne, J. & Lamp, W.O. (2009) Mitochondrial lineages and DNA barcoding of closely related species in the mayfly genus Ephemerella (Ephemeroptera: Ephemerellidae). Journal of the North American Benthological Society, 28, 584–595.
Ball, S.L., Hebert, P.D.N. & Burian, S.K.J.M. (2005) Biological identifications of mayflies (Ephemeroptera) using DNA barcodes. Journal of the North American Benthological Society, 24, 508–524.
Barrett, R.D.H. & Hebert, P.D.N. (2005) Identifying spiders through DNA barcodes. Canadian Journal of Zoology, 83, 481–491.
Boumans, L. & Baumann, R.W. (2012) Amphinemura palmeni is a valid Holarctic stonefly species (Plecoptera: Nemouridae). Zootaxa, 3537, 59–75.
Blaxter, M.L. (2004) The promise of a DNA taxonomy. Philosophical Transactions of the Royal Society of London B, 359, 669–679.
Bae, Y.J. & Cao, T.K.T. (2009) Phanoperla namcattien, a new stonefly from Vietnam (Plecoptera: Perlidae). Zootaxa, 1983, 66–68.
Claassen, P.W. (1940) A catalogue of the Plecoptera of the world. Memoirs of the Cornell University Agricultural Experiment Station. 232, 1–235.
Ekrem, T., Willassen, E. & Stur, E. (2007) A comprehensive DNA sequence library is essential for identification with DNA barcodes. Molecular Phylogenetics and Evolution, 43, 530–542.
Enderlein, G. (1909) Plecopterologische Studien II. Stettiner Entomologische Zeitung, Stettin. 70, 324–352.
Folmer O., Black, Hoeh, M., Lutz, W.R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase I. Molecular Marine Biology and Biotechnology, 3, 294–299.
Hebert, P.D.N., Cywinska, A., Ball, S.L. & deWaard, J.R. (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. B, 270, 313–321.
Hebert, P.D.N., Burns, E.H. J.M., Janzen, D.H. & Hallwachs, W. (2004) Ten species in one: DNA barcoding reveals cryptic species in the Neotropical skipper butterfly Astraptes fulgerator. Proceedings of the National Academy of Sciences, 101, 14812–14817.
Heinold, B.D., Gill, B.A., Belcher, T.P. & Verdone, C.J. (2014) Discovery of new populations and DNA barcoding of the Arapahoe snowfly Arsapnia arapahoe (Plecoptera: Capniidae). Zootaxa, 3866 (1), 131–137.
http://dx.doi.org/10.11646/zootaxa.3866.1.7Illies, J. (1966) Katalog der rezenten Plecoptera. Das Tierreich. 82, 1–632.
Lin, C.-P. & Wood, T.K. (2002) Molecular phylogeny of the North American Enchenopa binotata (Homoptera: Membracidae) species complex. Annals of the Entomological Society of America, 95, 162–171.
Paquin, P. & Hedin, M. (2004) The power and perils of ‘molecular taxonomy’: a case study of eyeless and endangered Cicurina (Araneae: Dictynidae) from Texas caves. Molecular Ecology, 13, 3239–3255.
Pauls, S.U., Blahnik, R.J., Zhou, X., Wardwell, C.T. & Holzenthal, R.W. (2010) DNA barcode data confirm new species and reveal cryptic diversity in Chilean Smicridea (Smicridea) (Trichoptera: Hydropsychidae). Journal of the North American Benthological Society, 29, 1058–1074.
Sivec, I. & Stark, B.P. (2010) Seven new species of Phanoperla Banks from Vietnam and Thailand (Plecoptera: Perlidae). Illiesia, 6, 98–112.
Sivec, I. & B.P. Stark. 2011. New species of Neoperla Needham and Phanoperla Banks (Plecoptera: Perlidae) from the Philippine Islands. Illiesia, 7, 264–279.
Sivec, I. & Yule, C. (2004) Insecta: Plecoptera. In: Freshwater Invertebrates of the Malaysian Region, C. M. Yule and H-S. Yong, eds. Academy of Sciences Malaysia. pp. 443–456.
Sivec, I., Stark, B.P. & Uchida, S. (1988) Synopsis of the world genera of Perlinae (Plecoptera: Perlidae). Scopolia, 16, 1–66.
Stark, B.P. (1983) Descriptions of Neoperlini from Thailand and Malaysia (Plecoptera: Perlidae). Aquatic Insects, 5, 99–114.
Stark, B.P. (1987) Records and descriptions of Oriental Neoperlini (Plecoptera: Perlidae). Aquatic Insects, 9, 45–50.
Stark, B.P. & Sheldon, A.L. (2009) Records of Neoperlini (Plecoptera: Perlidae) from Brunei Darussalam and Sarawak, with descriptions of new Phanoperla Banks and Neoperla Needham species. Illiesia, 5, 11–19.
Stark, B.P. & Sivec, I. (2007) Studies of Indonesian Perlidae (Plecoptera), with descriptions of three new species. Illiesia, 3, 53–64.
Stoeckle, M. (2003) Taxonomy, DNA, and the barcode of life. Biosciences, 53, 796–797.
Waringer, J., Graf, W., Pauls, S.U., Vicentini, H. & Lubini, V. (2008) DNA based association and description of the larval stage of Drusus melanchaetes McLachlan, 1876 (Trichoptera: Limnephilidae: Drusinae) with notes on ecology and zoogeography. Limnologica 38, 34–42.
Zhou, X., Kjer, K.M. & Morse, J.C. (2007) Associating larvae and adults of Chinese Hydropsychidae caddisflies (Insecta: Trichoptera) using DNA sequences. Journal of the North American Benthological Society, 26, 719–742.
Zwick, P. (1973b) Insecta: Plecoptera Phylogenetisches System und Katalog. Das Tierreich. 94, 1-465.
Zwick, P. (1982) A revision of the Oriental stonefly genus Phanoperla (Plecoptera: Perlidae). Systematic Entomology, 7, 87–126.
Zwick, P. (1983) Notes on Plecoptera (9) Neoperla fuscipennis Navás. Aquatic Insects, 5, 255–256.
Zwick, P. (1986) Contribution to the knowledge of Phanoperla Banks, 1938 (Plecoptera: Perlidae). Mitteilungen der Schweizerischen Gesellschaft: Bulletin de la Société Entomologique Suisse, 59, 151–158.
Zwick, P. & Sivec, I. (1985) Supplements to the Perlidae (Plecoptera) of Sumatra. Spixiana, 8, 123–133.