Abstract
The Epicopeiidae is a small geometroid family distributed in the East Palaearctic and Oriental regions. It exhibits high morphological diversity in body size and wing shape, while their wing patterns involve in various complex mimicry rings. In the present study, we attempted to describe a new genus, and a new species from Vietnam, with comments on two assumed congeneric novel species from China and India. To address its phylogenetic affinity, we reconstructed the phylogeny of the family by using sequence data of COI, EF-1α, and 28S gene regions obtained from seven genera of Epicopeiidae with Pseudobiston pinratanai as the outgroup. We also compared the morphology of the new taxon to other epicopeiid genera to affirm its taxonomic status. The results suggest that the undescribed taxon deserve a new genus, namely Mimaporia gen. n. The species from Vietnam, Mimaporia hmong sp. n., is described as new to science. Under different tree building strategies, the new genus is the sister group of either Chatamla Moore, 1881 or Parabraxas Leech, 1897. The morphological evidence, which was not included in phylogenetic analyses, however, suggests its potential affinity with Burmeia Minet, 2003. This study also provides the first, although preliminary, molecular phylogeny of the family on which the revised systematics and interpretation of character evolution can be based.
References
Birch, M.C. (1972) Male abdominal brush-organs in British noctuid moths and their value as a taxonomic character. Part I. The Entomologist, 105, 185–205.
Caterino, M.S. & Sperling, F.A.H. (1999) Papilio Phylogeny Based on Mitochondrial Cytochrome Oxidase I and II Genes. Molecular Phylogenetics and Evolution, 11, 122–137.
https://dx.doi.org/10.1006/mpev.1998.0549
Common, I.F.B. (1990) Moths of Australia. Melbourne University Press, Melbourne, 535 pp.
Fletcher, D.S. (1979) Geometroidea: Apoprogonidae, Axiidae, Callidulidae, Cyclidiidae, Drepanidae, Epicopeiidae, Epiplemidae, Geometridae, Pterothysanidae, Sematuridae, Thyatiridae, Uraniidae. In: Nye, I.W.B. (Ed.), The generic names of moths of the world. Vol. 3. Trustees of the British Museum (Natural History), London, pp. i–xx, 1–243 + 2 pls.
Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular marine biology and biotechnology, 3, 294–299.
Hall, T. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series, 41, 95–98.
Heikkilä, M., Mutanen, M., Wahlberg, N., Sihvonen, P. & Kaila, L. (2015) Elusive ditrysian phylogeny: an account of combining systematized morphology with molecular data (Lepidoptera). BMC Evolutionary Biology, 15, 260.
http://doi.org/10.1186/s12862-015-0520-0
Heppner, J.B. (2005) Oriental Swallowtail Moths (Lepidoptera: Epicopeiidae). In: Capinera, J.L. (Ed.), Encyclopedia of Entomology. Springer Netherlands, Dordrecht, pp. 1606.
Holloway, J., Bradley, J., Carter, D. & Betts, C. (1987) CIE guides to insects of importance to man. I. Lepidoptera. CAB International, London, 262 pp.
Holloway, J.D., Kibby, G. & Peggie, D. (2001) The Families of Malesian Moths and Butterflies. Brill, Leiden, 455 pp.
Klots, A.B. (1970) Lepidoptera. In: Tuxen, S.L. (Ed.), Taxonomist’s glossary of genitalia in insects. (Second revised and enlarged edition). Munksgaard, Copenhagen, pp. 115–130.
Laithwaite, E., Watson, A. & Whalley, P.E.S. (1975) The dictionary of butterflies and moths in colour. Michael Joseph, London, xlvi + 296 pp.
Miller, M.A., Pfeiffer, W. & Schwartz, T. (2010) Creating the CIPRES Science Gateway for inference of large phylogenetic trees. In: Proceedings of the Gateway Computing Environments Workshop (GCE). New Orleans, LA, pp. 1–8.
http://doi.org/10.1109/GCE.2010.5676129
Minet, J. (1983) Etude morphologique et phylogénétique des organes tympaniques des Pyraloidea. I. - Généralités et homologies (Lep. Glossata). Annales de la Société entomologique de France, 19, 175–207.
Minet, J. (1986) Ébauche d’une classification moderne de l’ordre des Lépidoptères. Alexanor, 14, 291–313.
Minet, J. (1991) Tentative Reconstruction of the Ditrysian Phylogeny (Lepidoptera, Glossata). Entomologica Scandinavica, 22, 69–95.
https://doi.org/10.1163/187631291X00327
Minet, J. (2003) The Epicopeiidae: phylogeny and a redefinition, with the description of new taxa (Lepidoptera: Drepanoidea). Annales de la Société Entomologique de France, 38, 463–487.
http://dx.doi.org/10.1080/00379271.2002.10697355
Niehuis, O., Yen, S.H., Naumann, C.M. & Misof, B. (2006) Higher phylogeny of zygaenid moths (Insecta: Lepidoptera) inferred from nuclear and mitochondrial sequence data and the evolution of larval cuticular cavities for chemical defence. Molecular Phylogenetics and Evolution, 39, 812–829.
http://dx.doi.org/10.1016/j.ympev.2006.01.007
Rajaei, H., Greve, C., Letsch, H., Stüning, D., Wahlberg, N., Minet, J. & Misof, B. (2015) Advances in Geometroidea phylogeny, with characterization of a new family based on Pseudobiston pinratanai (Lepidoptera, Glossata). Zoologica Scripta, 44, 418–436.
http://dx.doi.org/10.1111/zsc.12108
Ronquist, F., Teslenko, M., van derMark, P., Ayres, D.L., Darling, A., Höhna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic biology, 61, 539–542.
https://dx.doi.org/10.1093/sysbio/sys029
Scoble, M. (1992) The Lepidoptera. Form, function and diversity. Oxford University Press, Oxford, 420 pp.
Sihvonen, P., Mutanen, M., Kaila, L., Brehm, G., Hausmann, A. & Staude, H.S. (2011) Comprehensive Molecular Sampling Yields a Robust Phylogeny for Geometrid Moths (Lepidoptera: Geometridae). PLoS ONE, 6, e20356.
http://dx.doi.org/10.1371/journal.pone.0020356
Stamatakis, A. (2014) RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30, 1312–1313.
https://dx.doi.org/10.1093/bioinformatics/btu033
Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular biology and evolution, 30, 2725–2729.
https://dx.doi.org/10.1093/molbev/mst197
Thompson, J.D., Higgins, D.G. & Gibson, T.J. (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic acids research, 22, 4673–4680.
https://doi.org/10.1093/nar/22.22.4673
Wahlberg, N. & Wheat, C.W. (2008) Genomic outposts serve the phylogenomic pioneers: designing novel nuclear markers for genomic DNA extractions of Lepidoptera. Systematic biology, 57, 231–242.
https://dx.doi.org/10.1080/10635150802033006
Wei, C.H. & Yen, S.H. (2015) Molecular phylogeny of the Epicopeiidae moths shows extremely high plasticity of the morphology of a quasi-Batesian mimic. In: 2015 Congress of Animal Behavior & Ecology. Available from: https://www.researchgate.net/publication/272498090_Molecular_phylogeny_of_the_Epicopeiidae_moths_shows_extremely_hig
h_plasticity_of_the_morphology_of_a_quasi-Batesian_mimic (accessed 13 March 2017)Whiting, M.F. (2002) Mecoptera is paraphyletic: multiple genes and phylogeny of Mecoptera and Siphonaptera. Zoologica Scripta, 31, 93–104.
http://dx.doi.org/10.1046/j.0300-3256.2001.00095.x
Wu, C.G., Han, H.X. & Xue, D.Y. (2010) A pilot study on the molecular phylogeny of Drepanoidea (Insecta: Lepidoptera) inferred from the nuclear gene EF-1α and the mitochondrial gene COI. Bulletin of Entomological Research, 100, 207–216.
https://dx.doi.org/10.1017/S0007485309990162