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Issue: Vol. 4277 No. 3: 19 Jun. 2017
Type: Article
Published: 2017-06-19
DOI: 10.11646/zootaxa.4277.3.3
Page range: 369–385
Abstract views: 121
PDF downloaded: 2

Molecular operational taxonomic units of mosquitoes (Diptera: Culicidae) collected in high Andean mountain ecosystems of Antioquia, Colombia

Grupo de Investigación en Sistemática Molecular, Facultad de Ciencias, Universidad Nacional de Colombia, Sede Medellín, Calle 59A 63-20. Bloque 16, Laboratorio 102. Medellin, Colombia
Grupo de Microbiología Molecular, Escuela de Microbiología, Universidad de Antioquia, Calle 67 No. 53-108, off. 5-430, Medellín, Colombia
Grupo de Investigación en Sistemática Molecular, Facultad de Ciencias, Universidad Nacional de Colombia, Sede Medellín, Calle 59A 63-20. Bloque 16, Laboratorio 102. Medellin, Colombia
Grupo de Investigación en Sistemática Molecular, Facultad de Ciencias, Universidad Nacional de Colombia, Sede Medellín, Calle 59A 63-20. Bloque 16, Laboratorio 102. Medellin, Colombia
Grupo de Microbiología Molecular, Escuela de Microbiología, Universidad de Antioquia, Calle 67 No. 53-108, off. 5-430, Medellín, Colombia
Griffin Laboratory, Wadsworth Center, New York State Department of Health, Slingerlands, New York, United States of America Department of Biomedical Sciences, School of Public Health, State University of New York, Albany, New York, United States of America
Grupo de Investigación en Sistemática Molecular, Facultad de Ciencias, Universidad Nacional de Colombia, Sede Medellín, Calle 59A 63-20. Bloque 16, Laboratorio 102. Medellin, Colombia
Diptera molecular operational taxonomic units mosquitoes cox1 highlands ITS2 Colombia

Abstract

Accurate taxonomic identification of highland mosquito species may be complicated because of the lack of comprehensive regional morphological keys and taxonomic specialists, particularly for mosquitoes of medical or ecological importance. We applied a multi-locus approach to explore the diversity of genera/species collected, to define the Molecular Operational Taxonomic Units (MOTUs) and to perform phylogenetic clustering. Twenty MOTUs and three single sequences were revealed from 78 concatenated cox1 + ITS2 sequences, and the species name was allocated for five of these. This study provides molecular taxonomic information of culicid fauna present in high Andean mountain ecosystems in Antioquia, Colombia. However, future morphological and integrative taxonomic studies should be conducted to achieve the specific identity of all detected MOTUs.

 

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