Skip to main content Skip to main navigation menu Skip to site footer
Responsive image
Issue: Vol. 4286 No. 2: 30 Jun. 2017
Type: Article
Published: 2017-06-30
DOI: 10.11646/zootaxa.4286.2.10
Page range: 277–284
Abstract views: 58
PDF downloaded: 1

Morphological description and DNA barcoding of Hydrobaenus golovinensis sp. nov. (Diptera: Chironomidae: Orthocladiinae) from the Russian Far East

Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far East Branch of the Russian Academy of Sciences, 100 let Vladivostoku 159, 690022 Vladivostok, Russia.
Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far East Branch of the Russian Academy of Sciences, 100 let Vladivostoku 159, 690022 Vladivostok, Russia.
Far Eastern Federal University, Suhanova St. 8, 690950 Vladivostok, Russia.
Far Eastern Federal University, Suhanova St. 8, 690950 Vladivostok, Russia.
Diptera Chironomidae Hydrobaenus new species taxonomy DNA barcoding Russian Far East

Abstract

Illustrated descriptions of adult male, pupa and fourth instar larva, as well as DNA barcoding results of Hydrobaenus golovinensis sp. nov. in comparison with closely related species H. majus Makarchenko et Makarchenko and H. sikhotealinensis Makarchenko et Makarchenko from the Russian Far East are provided. Partial mitochondrial COI gene (DNA barcoding) of the new species has been sequenced and uploaded to GenBank. Comparisons with corresponding regions of COI between H. golovinensis and other species in the genus produce K2P genetic distances of 10.3–14.3%, the values well above those associated with intraspecific variation. In contrast, genetic distances among 18 specimens are all within the range of 0–3.5%. The ML tree is also constructed using DNA barcodes obtained in the present study and those of other species of Hydrobaenus Fries from GenBank.

 

References

  1. Carew, M.E., Pettigrove, V. & Hoffmann, A.A. (2005) The utility of DNA markers in classical taxonomy: using cytochrome oxidase I markers to differentiate Australian Cladopelma (Diptera: Chironomidae) midges. Annals of the Entomological Society of America, 98, 587–594.
    https://doi.org/10.1603/0013-8746(2005)098[0587:TUODMI]2.0.CO;2

    Ekrem, T., Stur, E. & Hebert, P.D.N. (2010) Females do count: Documenting Chironomidae (Diptera) species diversity using DNA barcoding. Organisms Diversity & Evolution, 10, 397–408.
    https://doi.org/10.1007/s13127-010-0034-y

    Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33 (7), 1870–1874.
    https://doi.org/10.1093/molbev/msw054

    Makarchenko, E.A. (1985) Chironomids of the Soviet Far East. Subfamilies Podonominae, Diamesinae and Prodiamesinae (Diptera, Chironomidae). DVNC AN SSSR Press, Vladivostok, 208 pp. [in Russian]

    Makarchenko, E.A., Makarchenko, M.A. & Yavorskaya, N.M. (2009) New records of chironomids (Diptera, Chironomidae, Orthocladiinae) in Far East and bordering territories. VI. Hydrobaenus Fries. Euroasian Entomological Journal, 8 (supplement 1), 33–50. [in Russian]

    Makarchenko, E.A. & Makarchenko, M.A. (2014) On taxonomy of Hydrobaenus Fries, 1830 (Diptera: Chironomidae: Orthocladiinae) from the Russian Far East, with a key to species. Zootaxa, 3760 (3), 429–438.
    https://doi.org/10.11646/zootaxa.3760.3.9

    Makarchenko, E.A., Makarchenko, M.A. & Semenchenko, A.A. (2015) Morphological description and DNA barcoding of Hydrobaenus majus sp. nov. (Diptera: Chironomidae: Orthocladiinae) from the Russian Far East. Zootaxa, 4000 (2), 287–293.
    https://doi.org/10.11646/zootaxa.4000.2.7

    Mikkelsen, P.M., Bieler, R., Kappner, I. & Rawlings, T.A. (2006) Phylogeny of Veneroidea (Mollusca: Bivalvia) based on morphology and molecules. Zoological Journal of the Linnean Society, 148 (3), 439–521.
    https://doi.org/10.1111/j.1096-3642.2006.00262.x

    Montagna, M., Mereghetti, V., Lencioni, V. & Rossaro, B. (2016) Integrated Taxonomy and DNA Barcoding of Alpine Midges (Diptera: Chironomidae), PLoS ONE, 11 (3), e0149673.
    https://doi.org/10.1371/journal.pone.0149673

    Sæther, O.A. (1980) Glossary of chironomid morphology terminology (Chironomidae, Diptera). Entomologica scandinavica, 14 (Supplement), 1–51.

    Silva, F.L. & Wiedenbrug, S. (2014) Integrating DNA barcodes and morphology for species delimitation in the Corynoneura group (Diptera: Chironomidae: Orthocladiinae). Bulletin of Entomological Research, 104 (1), 65–78.
    https://doi.org/10.1017/S0007485313000515