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Issue: Vol. 4363 No. 4: 13 Dec. 2017
Type: Article
Published: 2017-12-12
DOI: 10.11646/zootaxa.4363.4.7
Page range: 553–560
Abstract views: 114
PDF downloaded: 2

Identification of Neoceratitis asiatica (Becker) (Diptera: Tephritidae) based on morphological characteristics and DNA barcode

Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China
Ningxia Academy of Agriculture and Forestry Science, Yinchuan 750002, China
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China
Ningxia Academy of Agriculture and Forestry Science, Yinchuan 750002, China
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China Ningxia Academy of Agriculture and Forestry Science, Yinchuan 750002, China
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China
Ningxia Academy of Agriculture and Forestry Science, Yinchuan 750002, China
Department of Entomology, College of Plant Protection, China Agricultural University, Beijing 100193, China
Diptera Neoceratitis asiatica (Becker) identification morphological characteristics DNA barcode COI

Abstract

Neoceratitis asiatica (Becker), which especially infests wolfberry (Lycium barbarum L.), could cause serious economic losses every year in China, especially to organic wolfberry production. In some important wolfberry plantings, it is difficult and time-consuming to rear the larvae or pupae to adults for morphological identification. Molecular identification based on DNA barcode is a solution to the problem. In this study, 15 samples were collected from Ningxia, China. Among them, five adults were identified according to their morphological characteristics. The utility of mitochondrial DNA (mtDNA) cytochrome c oxidase I (COI) gene sequence as DNA barcode in distinguishing N. asiatica was evaluated by analysing Kimura 2-parameter distances and phylogenetic trees. There were significant differences between intra-specific and inter-specific genetic distances according to the barcoding gap analysis. The uncertain larval and pupal samples were within the same cluster as N. asiatica adults and formed sister cluster to N. cyanescens. A combination of morphological and molecular methods enabled accurate identification of N. asiatica. This is the first study using DNA barcode to identify N. asiatica and the obtained DNA sequences will be added to the DNA barcode database.

 

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