Abstract
A molecular analysis based on mitochondrial cytochrome oxidase subunit 1 (COI) gene sequences has indicated that larvae collected in Sichuan and Zhejiang Provinces, China, belong to Onycholyda xanthogaster Shinohara, 1999, and O. fulvicornis Shinohara, in Shinohara & Wei, 2016 (Hymenoptera, Pamphiliidae), and that a male Onycholyda specimen from Mt. Tianmushan, Zhejiang Province is the hitherto unknown male of O. tianmushana Shinohara & Xiao, 2006. The first host plant records are Rubus inopertus (Focke) Focke (Rosaceae) for O. xanthogaster and Rubus hirsutus Thunb. for O. fulvicornis. The larvae of O. xanthogaster and O. fulvicornis are briefly described and O. xanthogaster is newly recorded from Sichuan Province. The male of O. tianmushana is described for the first time.
References
Folmer, O., Black, M., Hoeh, W., Lutz, R. & Vrijenhoek, R. (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3, 294−299.
Kangas, J.K. & Syrjänen, A.J. (1962) Beobachtungen zur Biologie von Pamphilius sertatus Knw. (Hymenoptera, Pamphiliidae). Annales Entomologici Fennici, 28, 185–187.
Kumar, S., Stecher, G. & Tamura, K. (2016) MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for bigger datasets. Molecular Biology and Evolution, 30, 2725–2729.
https://doi.org/10.1093/molbev/msw054Lu, L.-D. & Boufford, D.E. (2003) 28. Rubus Linnaeus. In: Flora of China. Vol. 9. Available from: http://www.efloras.org/florataxon.aspx?flora_id=2&taxon_id=128837 (accessed 23 October 2017)
Schmidt, S., Taeger, A., Morinière, J., Liston, A., Blank, S. M., Kramp, K., Kraus, M., Schmidt, O., Heibo, E., Prous, M., Nyman, T., Malm, T. & Stahlhut, J. (2017) Identification of sawflies and horntails (Hymenoptera, ‘Symphyta’) through DNA barcodes: successes and caveats. Molecular Ecology Resources, 17, 670–685.
https://doi.org/10.1111/1755-0998.12614Shinohara, A. (1985) The sawfly genus Onycholyda (Hymenoptera, Pamphiliidae) of Japan I. Kontyû, Tokyo, 53, 346–359.
Shinohara, A. (1999) Leaf-rolling sawflies of the genus Onycholyda (Hymenoptera, Pamphiliidae) from Shaanxi Province, China. Bulletin of the National Science Museum, Tokyo, Series A, 25, 65–71.
Shinohara, A. (2002) Systematics of the leaf-rolling or webspinning sawfly subfamily Pamphiliinae (Hymenoptera): a preliminary overview. In: Viitasaari, M. (Ed.), Sawflies (Hymenoptera, Symphyta). I. Tremex Press, Helsinki, pp. 359–438.
Shinohara, A., Hara, H., Kramp, K., Blank, S. M. & Kameda, Y. (2017) Bird droppings on chestnut leaves or sawfly larvae? DNA barcodes verify the occurrence of the archaic Megaxyela togashii (Hymenoptera, Xyelidae) in Hokkaido, Japan. Zootaxa, 4221 (2), 220–232.
https://doi.org/10.11646/zootaxa.4221.2.6Shinohara, A. & Ibuki, S. (2014) Larva and life history of Onycholyda minomalis (Hymenoptera, Pamphiliidae) in Honshu, Japan. Bulletin of the National Museum of Nature and Science, Series A, 40, 215–218.
Shinohara, A., Kameda, Y., Ibuki, S., Kiyoshi, T., Kakuda, T. & Kojima, H. (2016a) Pamphilius ishikawai feeds on Astilbe: the first record of Pamphiliidae (Hymenoptera) associated with Saxifragaceae. Zootaxa, 4098 (1), 167–178.
https://doi.org/10.11646/zootaxa.4098.1.8Shinohara, A., Kiyoshi, T. & Kameda, Y. (2016b) New distribution records of two species of Arge and Spinarge (Hymenoptera, Argidae) from Kyushu, Japan. Bulletin of the National Museum of Nature and Science, Tokyo, Series A, 42, 81–85.
Shinohara, A., Kiyoshi, T., Wei, M.-C. & Kameda, Y. (2018) Identification of larvae and recognition of new synonymy for two Chinese Onycholyda sawflies (Hymenoptera, Pamphiliidae) by molecular data. Species Diversity, 23. [accepted January 15, 2018]
Shinohara, A. & Kojima, H. (2013) Discovery of host plant and larva of Onycholyda similis (Hymenoptera, Pamphiliidae) in Honshu, Japan. Japanese Journal of Systematic Entomology, 19, 107–112.
Shinohara, A. & Lee, H.-S. (2011) Host plants and larvae of two leaf-rolling sawfly species of the genus Onycholyda (Hymenoptera, Pamphiliidae) in Korea. Japanese Journal of Systematic Entomology, 17, 209–212.
Shinohara, A. & Wei, M.-C. (2010) Discovery of host plant and larva of Onycholyda odaesana (Hymenoptera, Pamphiliidae) in Hunan Province, China. Japanese Journal of Systematic Entomology, 16, 105–107.
Shinohara, A. & Wei, M.-C. (2012) Pamphiliid sawflies (Hymenoptera, Symphyta) of Mt. Yunshan, Hunan Province, China. Memoirs of the National Museum of Nature and Science, Tokyo, 48, 53–74.
Shinohara, A. & Wei, M.-C. (2016) Leaf-rolling sawflies (Hymenoptera, Pamphiliidae, Pamphiliinae) of Tianmushan Mountains, Zhejiang Province, China. Zootaxa, 4072 (3), 301–318.
https://doi.org/10.11646/zootaxa.4072.3.1Shinohara, A. & Xiao, G.-R. (2006) Some leaf-rolling sawflies (Hymenoptera, Pamphiliidae, Pamphiliinae) from China in the collection of the Research Institute of Forest Protection, Chinese Academy of Forestry, Beijing. In: Blank, S.M., Schmidt, S. & Taeger, A. (Eds.), Recent Sawfly Research: Synthesis and Prospects. Goecke & Evers, Keltern, pp. 285–296.
Taeger, A., Blank, S.M. & Liston, A.D. (2010) World catalog of Symphyta (Hymenoptera). Zootaxa, 2580, 1–1064.
https://doi.org/10.11646/zootaxa.2580.1Tamura, K. (1992) Estimation of the number of nucleotide substitutions when there are strong transition-transversion and G + C-content biases. Molecular Biology and Evolution, 9, 678–687.
Viitasaari, M. (2002) The suborder Symphyta of the Hymenoptera. In: Viitasaari, M. (Ed.), Sawflies (Hymenoptera, Symphyta) I. A review of the suborder, the Western Palaearctic taxa of Xyeloidea and Pamphilioidea. Tremex, Helsinki, pp. 11–174.