Abstract
The identity of many species of Anisepyris Kieffer is currently unknown, mostly because the available taxonomic data are not sufficiently accurate to allow for precise identifications. To solve this problem, all 121 described species were investigated, including the analyses of 115 holotypes and 26 allotypes. Almost all Neotropical species of Epyrinae and many from the Nearctic were also examined in order to recognize possible species of Anisepyris misclassified in other genera. The ten species-groups included in Anisepyris are redefined. And the female genital structures of the genus are investigated for the first time. Based on this, we recognize 119 previously described species as belonging to Anisepyris, one species is transferred to Laelius Ashmead, L. foveaticeps (Rosmann & Azevedo) comb. nov., one is transferred to Chlorepyris Kieffer, C. nigerrimus (Evans) comb. nov., and 135 new species are described to science, so that the genus is now contains 254 species. They are recorded from 31 countries of the New World. Lectotypes are designated for A. aurichalceus (Westwood) and A. planiceps (Fabricius). The previously identified species-groups are re-organized into six, and seven additional species-groups are proposed are new. Identification keys for Anisepyris species-groups and species are provided.
References
Ashmead, W.H. (1887) Studies on the North American Proctotrupidae with descriptions of new species from Florida. Entomologica Americana, 3, 73–76.
Ashmead, W.H. (1893) A monograph of the North American Proctotrypidae. Bulletin of the United States National Museum, 45,1–472.
https://doi.org/10.5479/si.03629236.45.1Ashmead, W.H. (1894) Report on the parasitic Cynipidae, part of the Braconidae, the Ichneumonidae, the Proctotrypidae, and part of the Chalcididae. Part III. Journal of Linnean Society of London, Zoology, 25, 188–254.
Azevedo, C.O. (1993) On Rhabdepyris Kieffer (Hymenoptera, Bethylidae) in the area of São Carlos, State of São Paulo, Brazil: II. Subgenus Trichotepyris. Revista Brasileira de Entomologia, 37, 305–308.
Barbosa, D.N. & Azevedo, C.O. (2009) Laelius Ashmead (Hymenoptera, Bethylidae) from Madagascar, with description of two new species. Zootaxa, 2170, 1–14.
Barbosa, D.N. & Azevedo, C.O. (2011) Phylogeny and Reclassification of Laelius (Hymenoptera; Bethylidae) and description of four new species. Insect Systematics and Evolution, 42, 237–275.
https://doi.org/10.1163/187631211X559777Barbosa, D.N. & Azevedo, C.O. (2014) Revision of the Neotropical Laelius (Hymenoptera: Bethylidae) with notes on some Nearctic species. Zoologia, 31, 285–311.
https://doi.org/10.1590/S1984-46702014000300012Brues, C.T. (1908) Notes and descriptions of North American parasitic Hymenoptera. VI. Bulletin of Wisconsin of the Natural History Society, 6, 48–56.
Cameron, P. (1888) Insecta. Hymenoptera (Families Tenthredinidae-Chrysididae). Subfamily Bethylinae. Biologica Centrali-Americana, 1, 448–457.
Colombo, W.D. & Azevedo, C.O. (2016) Review of Dissomphalus Ashmead, 1893 (Hymenoptera, Bethylidae) from Espírito Santo, Brazil, with description of twenty-one new species. Zootaxa, 4143 (1), 1–84.
https://doi.org/10.11646/zootaxa.4143.1.1Cresson, E.T. (1872) Hymenoptera Texana. Transactions of the American Entomological Society, 4, 153–292.
https://doi.org/10.2307/25076272Dallwitz, M.J., Paine, T.A. & Zurcher, E.J. (1993) User’s Guide to the DELTA System: a General System for Processing Taxonomic Descriptions. 4th Edition. Available from: http://biodiversity.uno.edu/delta/ (accessed 20 August 2006)
Duporte, E.M. (1965) The lateral and ventral sclerites of the insect thorax. Canadian Journal of Zoology, 43, 141–154.
https://doi.org/10.1139/z65-012Ernst, A., Mikó, I. & Deans, A.R. (2013) Morphology and function of the ovipositor mechanism in Ceraphronoidea (Hymenoptera: Apocrita). Journal of Hymenoptera Research, 33, 25–61.
https://doi.org/10.3897/jhr.33.5204Evans, H.E. (1959a) The genus Anisepyris in the Greater Antilles (Hymenoptera, Bethylidae). Bulletin of the Brooklyn Entomological Society, 54, 69–75.
Evans, H.E. (1959b) The Nearctic species of Lophepyris, a new subgenus of Rhabdepyris (Hymenoptera, Bethylidae). Proceedings of the Entomological Society of Washington, 61, 201–204.
Evans, H.E. (1961) A revision of the genus Pseudisobrachium in the North and Central America (Hymenoptera, Bethylidae). Bulletin of the Museum of Comparative Zoology, 126, 211–318.
Evans, H.E. (1964) A synopsis of the American Bethylidae (Hymenoptera, Aculeata). Bulletin of the Museum of Comparative Zoology, 132, 183–189.
Evans, H.E. (1965) A revision of the genus Rhabdepyris in the Americas (Hymenoptera: Bethylidae). Bulletin of the Museum of Comparative Zoology, 133, 68–151.
Evans, H.E. (1966) A revision of the genus Anisepyris Kieffer (Hymenoptera. Bethylidae). Studia Entomologica, 9, 1–120.
Evans, H.E. (1967) Notes on Mexican and Southwestern U. S. Bethylidae (Hymenoptera). Part I. Pristocerinae. Entomological News, 78, 13–23.
Evans, H.E. (1969) A revision of the genus Epyris in the Americas (Hymenoptera, Bethylidae). Transactions of the American Entomological Society, 95, 181–352.
Evans, H.E. (1970) West Indian wasps of the subfamilies Epyrinae and Bethylinae (Hymenoptera: Bethylidae). Proceedings of the Entomological Society of Washington, 72, 340–356.
Evans, H.E. (1979) Additions to knowledge of the bethylid fauna of Hispaniola (Hymenoptera: Bethylidae). Proceedings of the Entomological Society of Washington, 81, 456–459.
Fabricius, J.C. (1804) Systema Piezatorum secundum ordines, genera, species, adiectis synonymis, locis, observationibus, descriptionibus. Carolum Reichard, Brunsvigae, 439 pp.
Fouts, R.M. (1928) Descriptions of new Nearctic Serphoidea (Hymenoptera). Proceedings of the Entomological Society of Washington, 29, 165–179.
Gibson, G.A.P. (1986) Mesothoracicskeletomusculature and mechanics of flight and jumping in Eupelminae (Hymenoptera, Chalcidoidea: Eupelmidae). The Canadian Entomologist, 118, 691–728.
https://doi.org/10.4039/Ent118691-7Gobbi, F.T & Azevedo, C.O. (2010) Taxonomia de Pseudisobrachium (Hymenoptera, Bethylidae) da Mata Atlântica Brasileira. Revista Brasileira de Entomologia, 54, 173–224.
https://doi.org/10.1590/S0085-56262010000200003Goulet, H. & Huber, J.T. (1993) Hymenoptera of the world: An identification guide to families. Research Branch Agriculture Canada, Ottawa, 668 pp.
Harris, R.A. (1979) A glossary of surface sculpturing. Occasional Papers in Entomology, 28, 1–31.
Kawada, R., Lanes, G.O. & Azevedo, C.O. (2015) Evolution of metapostnotum in flat wasps (Hymenoptera, Bethylidae): implications for homology assessments in Chrysidoidea. PLoS ONE, 10 (10), e0140051.
https://doi.org/10.1371/journal.pone.0140051Kawada, R. & Buffington, M.L. (2016) A Scalable and Modular Dome Illumination System for Scientific Microphotography on a Budget. PLoS ONE, 11 (5), e0153426.
https://doi.org/10.1371/journal.pone.0153426Kieffer, J.J. (1905) Description de nouveaux Hyménoptères exotiques. Bulletin de la Société d'Histoire Naturelle de Metz, 24, 85–114.
Kieffer, J.J. & Marshall, T.A. (1904–1906) Proctotrypides. In: André, E. (Ed.), Species des Hyménoptères d’Europe & d’Algerie. Tome IX. Librairie Scientifique A. Hermann, Paris, pp. 1–551, 21 planches. [publication dates of the different parts: pages 1–64 1st January 1904; 65–144 1st April 1905; 145–288 1st November 1905; 289–368 31st January 1906; 369 à la fin 1st October 1906]
Kieffer, J.J. (1905) Beschreibung neuer Proctotrypiden aus Nord-und Zentralamerika. Berliner Entomologische Zeitschrift, 50, 237–290.
Kieffer, J.J. (1906) Description de nouveaux Hyménoptères. Annales de la Societe Scientifique de Bruxelles, 30, 113–178.
Kieffer, J.J. (1908) Nouveaux proctotrypides et cynipides d'Amérique recueillis par M. Baker. Annales de la Société Scientifique de Bruxellas, 32, 7–64.
Kieffer, J.J. (1908) Bethylidae. In: Wytsman, P. (Ed.), Genera Insectorum, 76, pp. 1–50.
Kieffer, J.J. (1910) Description de nouveaux Béthylides (Hymen). Annales de la Société Entomologique de France, 79, 31–56.
Kieffer, J.J. (1913) Nouveaux microhyménoptères de l'Afrique équatoriale. Bollettino di Laboratorio di Zoologia Generale e Agraria della R.Scuola Superiore d'Agricoltura in Portici, 7, 105–112.
Kieffer, J.J. (1914) Bethylidae. Das Tierreich, 41, 228–595.
Martinelli, A.B., Waichert, C., Barbosa, D.N., Fagundes, V. & Azevedo, C.O. (2017) The use of Proteinase K to access genitalia morphology, vouchering and DNA extraction in minute wasps. Anais da Academia Brasileira de Ciências, 89 (3), 1629–1633.
https://doi.org/10.1590/0001-3765201720160825Mikó, I., Vilhelmsen, L., Johnson, N.F., Masner, L.& Pénzes, Z. (2007) Skelomusculature of Scelionidae (Hymenoptera: Platygastroidea): Head. Zootaxa, 1571, 1–78.
Moreira, A.R. & Azevedo, C.O. (2003) Taxonomy of Anisepyris (Hymenoptera, Bethylidae) from Estação Biológica de Santa Lúcia, Santa Teresa, ES, Brazil. Boletim do Museu de Biologia Mello Leitão, 15, 35–39.
Muesebeck, C.F.W. & Walkley, L.M. (1951) Family Bethylidae. In: Muesebeck, C.F.W., Krombein, K.V. & Townes, H.K. (Eds.), Hymenoptera of America North of Mexico: Synoptic Catalog. Agricultural Monograph. United States Department of Agriculture, Washington, D.C., pp. 726–734.
Ramos, M.S. & Azevedo, C.O. (2016) Afrobethylus Ramos & Azevedo, gen. nov., a new remarkable Afrotropical genus of Bethylinae (Hymenoptera: Bethylidae). Zootaxa, 4097 (4), 495–510.
https://doi.org/10.11646/zootaxa.4097.4.3Richards, O.W. (1977) Hymenoptera Introduction and key to families. In: Handbooks for the identification of British Insects. Vol. 4. Royal Entomological Society of London, Queens Gate, London, pp. 98.
Ronquist, F. & Nordlander, G. (1989) Skeletal morphology of an archaic cynipoid, Ibaliarufipes (Hymenoptera: Ibaliidae). Entomologica Scandinavica, 33, 1–60.
Rosmann, W.L. & Azevedo, C.O. (2005) Dez espécies novas e notas sobre Rhabdepyris Kieffer (Hymenoptera, Bethylidae) da Mata Atlântica. Revista Brasileira de Entomologia, 49, 397–408.
https://doi.org/10.1590/S0085-56262005000300009Santos, H.S. (2002) Species of Anisepyris Kieffer, 1905 (Hymenoptera, Bethylidae) collected in Cachoeira da Fumaça and Forno Grande Parks, Espírito Santo, Brazil. Revista Brasileira de Entomologia, 46, 243–249.
https://doi.org/10.1590/S0085-56262002000300003Santos, H.S. & Azevedo, C.O. (2000) Sistemática das espécies de Anisepyris Kieffer (Hymenoptera, Bethylidae) do Brasil. Revista Brasileira de Entomologia, 44, 129–165.
Santos, L.M. & Azevedo, C.O. (2008) Taxonomia de Anisepyris Kieffer (Hymenoptera, Bethylidae) da Mata Atlântica Brasileira. Revista Brasileira de Entomologia, 52, 328–339.
https://doi.org/10.1590/S0085-56262008000300004Snelling, R.R. (1996) Systematic notes on some Bethylidae from the Virgin Islands and Puerto Rico (Hymenoptera: Chrysidoidea). Memoirs of the Entomological Society of Washington, 17, 194–208.
Sorg, M. (1988) Zur Phylogenie und Systematik der Bethylidae (Insecta, Hymenoptera, Chrysidoidea). Geologisches Institut der Universitaet zu Koeln Sonderveroeffentlichungen, 63, 1–146.
Terayama, M. (2003) Phylogenetic Systematics of the family Bethylidae (Insecta: Hymenoptera). Part I. Higher classification. The Academic Reports of the Faculty of Engineering of Tokyo Polytechnic University, 26, 1–15.
Tolkien, J.R.R. (1977) The Silmarillion. Ballantine Book, New York, xiv + 458 pp.
Waichert, C. & Azevedo, C.O. (2009) Phylogenetic analysis of Rhabdepyris (Hymenoptera: Bethylidae) and redefinition of generic limits based on structural characters. Zootaxa, 2284, 1–29.
Westwood, J.O. (1874) Thesaurus Entomologicus Oxoniensis. Clarendon Press, Oxford, 205 pp.