Abstract
Phyllocnistis podocarpa sp. nov., is described from mines in Podocarpus macrophyllus (Family Podocarpaceae). The host plant P. macrophyllus, also known as buddhist pine on the IUCN Red List, is a noticeable garden plant and thus of high economic value. Buddhist pine has been introduced to many other countries from its native habitat in southern Japan. Special attention has been paid for it during the overseas import in China. The morphology of the pupae of P. podocarpa, particularly the frontal process of the head and the spine clusters on terga, ones of the most useful diagnostic characters for species identification of Phyllocnistis on morphology, is demonstrated using SEM. Two parasitoid species of Eulophidae (Hymenoptera) are identified and illustrated. COI barcode sequences are provided along with a Neighbor Joining Tree covering related species for aiding identification.
References
Brito, R., Gonçalves, G.L., Vargas, H.A. & Moreira, G.R.P. (2012) A new species of Phyllocnistis Zeller (Lepidoptera: Gracillariidae) from southern Brazil, with life-history description and genetic comparison to congeneric species. Zootaxa, 3582, 1−16.
Brito R., Lopez-Vaamonde C., Gonçalves G.L., Becker V.O., Mielke O.H.H. & Moreira G.R.P. (2017a) Taxonomic revision of Neotropical Phyllocnistis Zeller, 1848 (Lepidoptera: Gracillariidae), with descriptions of seven new species and host plant associations. Zootaxa, 4341 (3), 301−352.
https://doi.org/10.11646/zootaxa.4341.3.1Brito R., Mielke O.H.H., Gonçalves G.L. & Moreira G.R.P. (2017b) Description of three new species of Phyllocnistis Zeller, 1848 (Lepidoptera: Gracillariidae), from the Atlantic Forest, south Brazil, with notes on natural history and phylogeny. Austral Entomology. [published online]
https://doi.org/10.1111/aen.12298Davis, D. & Wagner, D. (2011) Biology and systematics of the New World Phyllocnistis Zeller leafminers of the avocado genus Persea (Lepidoptera, Gracillariidae). ZooKeys, 97, 39−73.
https://doi.org/10.3897/zookeys.97.753De Prins, J. & De Prins, W. (2018) Global taxonomic database of Gracillariidae (Lepidoptera). Available from: http://www.gracillariidae.net/ (accessed 15 April 2018)
Farjon, A. (2013) Podocarpus macrophyllus. Available from: http://www.iucnredlist.org/details/42517/0 (accessed 03 December 2017)
Fochezato, J., Brito, R., Isaias, R.M.dos S., Gonçalves, G.L. & Moreira, G.R.P. (2017) Phyllocnistis hemera sp. nov. (Lepidoptera: Gracillariidae): a new species of leaf-miner associated with Daphnopsis fasciculata (Thymelaeaceae) in the Atlantic Forest. Revista Brasileira de Entomologia, 62, 57–65.
https://doi.org/10.1016/j.rbe.2017.11.001Forbes W.T.M. (1923) Lepidoptera of New York and neighboring States. Part I. Primitive forms, Microlepidoptera, Pyraloids, Bombyces. Memoirs of Cornell University Agricultural Experimental Station, 68, 1−729. Available from: https://www.biodiversitylibrary.org/item/65029 (Accessed 20 May 2018)
Fu, L., Li, Y. & Mill, R.R. (1999) Podocarpaceae. In: Wu, Z., Raven, P.H. & Hong, D. (Eds.), Flora of China. Science Press and Missouri Botanical Garden Press, Beijing, St. Louis, pp. 78−84. Available from: http://www.efloras.org/florataxon.aspx?flora_id=2&taxon_id=10713 (Accessed 20 May 2018)
Hebert P.D., Cywinska A. & Ball S.L. (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society of London B: Biological Sciences, 270 (1512), 313−321.
https://doi.org/10.1098/rspb.2002.2218Kirichenko, N., Triberti, P., Kobayashi, S., Hirowatari, T., Doorenweerd, C., Ohshima, I., Huang, G.-H., Wang, M., Magnoux, E. & Lopez-Vaamonde, C. (2018) Systematics of Phyllocnistis leaf-mining moths (Lepidoptera, Gracillariidae) feeding on dogwood (Cornus spp.) in Northeast Asia, with the description of three new species. ZooKeys, 736, 79−118.
https://doi.org/10.3897/zookeys.736.20739Kobayashi S. & Hirowatari T. (2011) Two Chloranthaceae leaiminer of the genus Phyllocnistis (Lepidoptera: Gracillariidae: Phyllocnistinae) from Japan ,with descriptions of new species and pupal morphology. Lepidoptera Science, 62 (4), 156−165.
https://doi.org/10.18984/lepid.62.4_156Kobayashi S., Sakamoto Y., Jinbo U., Nakamura A. & Hirowatari T. (2011) A new willow leaf blotch miner of the genus Phyllocnistis (Lepidoptera: Gracillariidae: Phyllocnistinae) from Japan, with pupal morphology and genetic comparison of Salicaceae mining species using DNA barcodes. Lepidoptera Science, 62 (2), 75−93.
https://doi.org/10.18984/lepid.62.2_75Langmaid J.R. & Corley M.F.V. (2007) Phyllocnistis ramulicola sp. nov. (Lepidoptera: Gracillariidae) in England and Portugal. Entomologist's Gazette, 58, 227−237.
Li H. (2002) The Gelechiidae of China (I) (Lepidoptera: Gelechioidea). Nankai University Press, Tianjin, 504 pp.
Liu T. & Wang S. (2017) First report of the leaf-mining genus Antispila Hübner, [1825] from mainland China, with the description of a new species feeding on Cornus (Lepidoptera, Heliozelidae). ZooKeys, 686, 95−107.
https://doi.org/10.3897/zookeys.686.13680Meyrick E. (1912–1916) Exotic Microlepidoptera. Thornhanger, Marlborough, Wilts, 640 pp. Available from: https://www.biodiversitylibrary.org/item/39853 (Accessed 20 May 2018)
Meyrick E. (1916−1923) Exotic Microlepidoptera. Thornhanger, Marlborough, Wilts, 640 pp. Available from: http://www.biodiversitylibrary.org/item/37719 (Accessed 20 May 2018)
Meyrick E. (1923−1930) Exotic Microlepidoptera. Thornhanger, Marlborough, Wilts, 640 pp.
Meyrick E. (1930−1936) Exotic Microlepidoptera. Thornhanger, Marlborough, Wilts, 642 pp.
Meyrick E. (1936−1937) Exotic Microlepidoptera. Thornhanger, Marlborough, Wilts, 160 pp.
Mourase M. (2005) Moths observed injuring Podocarpus macrophyllus (Podocarpaceae) in the Wakayama area. The Japan Heterocerists' Journal, 236, 201−203.
Nakamura N. (1993) Immature stages of a Phylocnistis (Phyllocnistidae) mining into the leaf of Podocarpus macrophyllus, a conifer tree. Japan Heterocerists' Journal, 174, 426−429.
Ratnasingham S. & Hebert P.D.N. (2007) BOLD: The barcode of life data system. Molecular Ecology Notes, 7 (3), 355−364.
https://doi.org/10.1111/j.1471-8286.2007.01678.xThe Plant List (2013) Version 1.1. Published on the Internet. Available from: http://www.theplantlist.org/ (accessed 21 November 2017)