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Type: Article
Published: 2018-06-28
Page range: 447–466
Abstract views: 112
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An updated phylogeny and morphological study of the Phymaturus vociferator clade (Iguania: Liolaemidae)

Programa de Fisiología y Biofísica, Facultad de Medicina, Universidad de Chile, Independencia 1027, Casilla 70005, Santiago, Chile.
Departamento de Biogeografía y Cambio Global, Museo Nacional de Ciencias Naturales, CSIC & Laboratorio Internacional en Cambio Global CSIC-PUC (LINCGlobal), Calle José Gutiérrez Abascal, 2, 28006, Madrid, Spain. Centro Iberoamericano de Biodiversidad CIBIO, Universidad de Alicante, Campus Universitario San Vicente del Raspeig s/n, 03690 Alicante.
Departamento de Biología, Facultad de Ciencias Exactas, Físicas y Naturales (FCEFyN), Universidad Nacional Universidad Nacional de San Juan—Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Av. José I. de la Roza 590 (0). J5402DCS, Rivadavia, San Juan, Argentina.
Departamento de Herpetología, Museo de Historia Natural de San Marcos (MUSM), Av. Arenales 1256, Jesús María, Lima, Perú. Instituto de Ciencias Biológicas Antonio Raimondi, Facultad de Ciencias Biológicas, Universidad Nacional Mayor de San Marcos, Lima, Perú.
Reptilia lizard Phymaturus palluma Chile Andes multilocus new species

Abstract

Species delimitation in Phymaturus has been a difficult task due to the highly conserved morphological and ecological features present in this genus. Almost all species of Phymaturus have been described without DNA data or lacking statistical analyses which makes even more difficult to compare species. Although two molecular phylogenetic studies have been recently published, here we provide the first multilocus phylogenetic reconstruction including all Chilean species, with samples from all type localities and some previously unsampled populations. We also estimate pairwise distances among the Chilean species of Phymaturus (P. vociferator and P. mallimaccii clades) and compare our results with the P. payuniae clade, where previous studies have used multiple lines of evidence. Additionally, we performed univariate and multivariate morphological analyses and skeletal comparisons (clavicle) for the species of the P. vociferator clade. As a result of this integrative approach, we describe a new species.

 

References

  1. Abdala, C.S. & Quinteros, A.S. (2014) Los últimos 30 años de estudios de la familia de lagartijas más diversa de Argentina. Actualización taxonómica y sistemática de Liolaemidae. Cuadernos de Herpetología, 28, 55–82.

    Aguilar, C., Wood Jr., P.L., Cusi, J.C., Guzmán, A., Huari, F., Lundberg, M., Mortensen, E., Ramírez, C., Robles, D., Suarez, J., Ticona, A., Vargas, V.J., Venegas, P. & Sites Jr., J.W. (2013) Integrative taxonomy and preliminary assessment of species limits in the Liolaemus walkeri complex (Squamata, Liolaemidae) with descriptions of three new species from Peru. Zookeys, 364, 47–91.
    https://doi.org/10.3897/zookeys.364.6109

    Aguilar, C., Wood Jr., P.L., Duff, M., Belk, M. & Sites Jr., J.W. (2017) Different roads lead to Rome: Integrative taxonomic approaches lead to the discovery of two new lizard lineages in the Liolaemus montanus group (Squamata: Liolaemidae). Biological Journal of the Linnean Society, 120, 448–467.

    https://doi.org/10.1111/bij.12890

    Avila, L.J., Morando, M., Pérez, D.R. & Sites Jr., J.W. (2009) A new species of Liolaemus from Añelo sand dunes, northern Patagonia, Neuquén, Argentina, and molecular phylogenetic re-lationships of the Liolaemus wiegmannii species group (Squamata, Iguania, Liolaemini). Zootaxa, 2234, 39–55.

    Avila, L.J., Fulvio Pérez, C.H., Morando, M. & Sites Junior, J.W. (2010) A new species of Liolaemus (Reptilia: Squamata) from southwestern Rio Negro province, northern Patagonia, Argentina. Zootaxa, 2434, 47–59.

    Breitman, M.F., Avila, L.J., Sites Junior, J.W. & Morando, M. (2012) How lizards survived blizzards: phylogeography of the Liolaemus lineomaculatus group (Liolaemidae) reveals multiple breaks and refugia in southern Patagonia, and their concordance with other co-distributed taxa. Molecular Ecology, 25, 6068–6085.
    https://doi.org/10.1111/mec.12075

    Cei, J.M. (1986) Reptiles del centro, centro-oeste y sur de la Argentina: Herpetofauna de las zonas áridas y semiáridas. Monografie IV. Museo Regionale di Scienze Naturali Torino, 1986, 1–527.

    Cei, J.M. (2003) Specific supraocular scutellation patterns as significant diagnostic characters: a taxonomic inter and intrageneric "finger-print" in Lacertilia. Facena, 19, 129–135.

    Cruz, F.B., Belver, L., Acosta, J.C., Villavicencio, H.J., Blanco, G. & Cánovas, M.G. (2009) Thermal biology of Phymaturus lizards: evolutionary constraints or lack of environmental variation? Zoology, 112, 425–432.
    https://doi.org/10.1016/j.zool.2009.03.004

    Darriba, D., Taboada, G.L., Doallo, R. & Posada, D. (2012) jModelTest 2: More models, new heuristics and parallel computing. Nature Methods, 9, 772.
    https://doi.org/10.1038/nmeth.2109

    Donoso-Barros, R. (1966) Reptiles de Chile. Ediciones de la Universidad de Chile, Santiago, cxliv + 458 pp.

    Dunn, O.J. (1964) Multiple comparisons using rank sums. Technometrics, 6, 241–252.
    https://doi.org/10.1080/00401706.1964.10490181

    Edgar, R.C. (2004) MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research, 32, 1792–1797.
    https://doi.org/10.1093/nar/gkh340

    Espinoza, R.E., Wiens, J.J. & Tracy, C.R. (2004) Recurrent evolution of herbivory in small, cold–climate lizards: breaking the ecophysiological rules of reptilian herbivory. Proceedings of the National Academy of Sciences, 101, 16819–16824.
    https://doi.org/10.1073/pnas.0401226101

    Esquerré, D., Núñez, H. & Scolaro, J.A. (2013) Liolaemus carlosgarini and Liolaemus riodamas (Squamata: Liolaemidae), two new species of lizards lacking precloacal pores, from Andean areas of Central Chile. Zootaxa, 3619 (4), 428–452.
    https://doi.org/10.11646/zootaxa.3619.4.2

    Esquerré, D., Troncoso-Palacios, J., Garín, C.F. & Núñez, H. (2014) The missing leopard lizard: Liolaemus ubaghsi sp. nov., a new species of the leopardinus clade (Reptilia: Squamata: Liolaemidae) from the Andes of the O’Higgins Region in Chile. Zootaxa, 3815 (4), 507–525.
    https://doi.org/10.11646/zootaxa.3815.4.3

    Etheridge, R.E. (1995) Redescription of Ctenoblepharys adspersa Tschudi, 1845, and the taxonomy of Liolaeminae (Reptilia: Squamata: Tropiduridae). American Museum Novitates, 3142, 1–34.

    Ferri-Yáñez, F. (2016) Thermal Niche Evolution in Liolaemus and Iberian lizards. Phd Thesis, Universidad de Alicante.

    Garin, C.F., Troncoso-Palacios, J., Ferri-Yáñez, F & Lobos, G. (2013) Liolaemus flavipiceus Cei & Videla 2003: primer registro en Chile y nuevos antecedentes para el taxón (Reptilia, Sauria, Liolaemidae). Cuadernos de Herpetología, 27, 65–69.

    González-Marín, A., Morando, M. & Avila, L.J. (2016a) Morfología lineal y geométrica en un grupo de lagartijas patagónicas del género Phymaturus (Squamata: Liolaemini). Revista Mexicana de Biodiversidad, 87, 399–408.
    https://doi.org/10.1016/j.rmb.2016.04.009

    González-Marín, A., Pérez, C.H.F., Minoli, I., Morando, M. & Avila, L.J. (2016b) A new lizard species of the Phymaturus patagonicus group (Squamata: Liolaemini) from northern Patagonia, Neuquén, Argentina. Zootaxa, 4121 (4), 412–430.
    https://doi.org/10.11646/zootaxa.4121.4.3

    Guindon, S. & Gascuel, O. (2003) A simple, fast and accurate method to estimate large phylogenies by maximum-likelihood. Systematic Biology, 52, 696–704.
    https://doi.org/10.1080/10635150390235520

    Hillis, D.M. & Bull, J.J. (1993) An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Systematic Biology, 42, 182–192.
    https://doi.org/10.1093/sysbio/42.2.182

    Librado, P. & Rozas, J. (2009) DnaSP. Version 5. A software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25, 1451–1452.
    https://doi.org/10.1093/bioinformatics/btp187

    Lobo, F. & Abdala, C.S. (2007) Descripción de una nueva especie de Phymaturus del grupo de P. palluma de la provincia de Mendoza, Argentina. Cuadernos de Herpetología, 21, 103–113.

    Lobo, F. & Etheridge, R. (2013) The type locality of Phymaturus palluma (Molina, 1782) (Reptilia: Iguania: Liolaemidae) and the status of Phymaturus adrianae Pereyra, 1992 and other unnamed populations. South American Journal of Herpetology, 8, 89–101.
    https://doi.org/10.2994/SAJH-D-12-00033.1

    Lobo, F. & Nenda, S.J. (2015) Discovery of two new species of Phymaturus (Iguania: Liolaemidae) from Patagonia, Argentina, and occurrence of melanism in the patagonicus group. Cuadernos de Herpetología, 29, 5–25.

    Lobo, F. & Quinteros, S. (2005) A morphological approach on the phylogenetic relationships within the genus Phymaturus (Iguania: Liolaemidae) with the description of four new species from Argentina. Papéis Avulsos de Zoologia, 45, 143–177.
    https://doi.org/10.1590/S0031-10492005001300001

    Lobo, F., Abdala, C.S. & Valdecantos, S. (2010) Taxonomic studies of the genus Phymaturus (Iguania: Liolaemidae): description of four new species. South American Journal of Herpetology, 5, 102–126.
    https://doi.org/10.2994/057.005.0205

    Lobo, F., Abdala, C.S. & Valdecantos, S. (2012a) Morphological diversity and phylogenetic relationships within a South-American clade of iguanian lizards (Liolaemidae: Phymaturus). Zootaxa, 3315, 1–41.

    Lobo, F., Nenda, S.J. & Slodki, D. (2012b) A new lizard of Phymaturus (Iguania: Liolaemidae) from Argentina. Herpetologica, 68, 121–133.
    https://doi.org/10.1655/HERPETOLOGICA-D-11-00044.1

    Lobo, F., Espinoza, R.E., Sanabria, E. & Quiroga, L. (2012c) A new Phymaturus (Iguania: Liolaemidae) from the southern extreme of the Argentine puna. Copeia, 2012, 12–22.
    https://doi.org/10.1643/CH-11-086

    Lobo, F., Laspiur, A. & Acosta, J.C. (2013) Description of new Andean species of the genus Phymaturus (Iguania: Liolaemidae) from Northwestern Argentina. Zootaxa, 3683 (2), 117–132.
    https://doi.org/10.11646/zootaxa.3683.2.2

    Lobo, F., Barrasso, D.A., Hibbard, T. & Basso, N.G. (2016) On the evolution and diversification of an Andean clade of reptiles: combining morphology and DNA sequences of the palluma group (Liolaemidae: Phymaturus). Zoological Journal of the Linnean Society, 176, 648–673.
    https://doi.org/10.1111/zoj.12335

    Maddison, W.P. & Maddison, D.R. (2017) Mesquite: a modular system for evolutionary analysis. Version 3.31. Available from: http://mesquiteproject.org (accessed 11 May 2018)

    Medina, C.D., Avila, L.J., Sites Junior, J.W. & Morando, M. (2014) Multilocus phylogeography of the Patagonian lizard complex Liolaemus kriegi (Iguania: Liolaemini). Biological Journal of the Linnean Society, 113, 256–269.
    https://doi.org/10.1111/bij.12285

    Morando, M., Avila, L.J., Turner, C.R. & Sites Junior, J.W. (2007) Molecular evidence for a species complex in the patagonian lizard Liolaemus bibronii and phylogeography of the closely related Liolaemus gracilis (Squamata: Liolaemini). Molecular Phylogenetics and Evolution, 43, 952–973.
    https://doi.org/10.1016/j.ympev.2006.09.012

    Morando, M., Avila, L.J., Pérez, C.H., Hawkins, M.A. & Sites Junior, J.W. (2013) A molecular phylogeny of the lizard genus Phymaturus (Squamata, Liolaemini): implications for species diversity and historical biogeography of southern South America. Molecular Phylogenetics and Evolution, 66, 694–714.
    https://doi.org/10.1016/j.ympev.2012.10.019

    Núñez, H. & Jaksic, F. (1992) Lista comentada de los reptiles terrestres de Chile continental. Boletín del Museo Nacional de Historia Natural de Chile, 43, 73–91.

    Núñez, H., Veloso, A., Espejo, P., Veloso, C., Cortés, A. & Araya, S. (2010) Nuevas especies de Phymaturus (grupo palluma) para la zona Cordillerana Central de Chile (Reptilia, Sauria, Liolaemidae). Boletín del Museo Nacional de Historia Natural de Chile, 59, 41–74.

    Pincheira-Donoso, D. (2004) Una nueva especie del género Phymaturus (Iguania: Tropiduridae: Liolaemini) del centro-sur de Chile. Multequina, 13, 57–70.

    Pincheira-Donoso, D. & Núñez, H. (2005) Las especies chilenas del género Liolaemus Wiegmann, 1834 (Iguania: Tropiduridae: Liolaeminae). Taxonomía, sistemática y evolución. Publicación Ocasional del Museo Nacional de Historia Natural de Chile, 59, 7–486.

    Ronquist, F., Teslenko, M., van der Mark, P., Ayres, D.L., Darling, A., H€ohna, S., Larget, B., Liu, L., Suchard, M.A. & Huelsenbeck, J.P. (2012) MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology, 61, 539–542.
    https://doi.org/10.1093/sysbio/sys029

    RStudio Team (2015) RStudio: Integrated development for R. RStudio, Inc., Boston, MA. Available from: http://www.rstudio.com/ (accessed 10 May 2017)

    Scolaro, J. (2010) Redescripción del neotipo de Phymaturus palluma: un aporte a la delimitación de su tierra típica (Reptilia, Sauria, Liolaemidae). Boletín del Museo Nacional de Historia Natural de Chile, 59, 29–39.

    Scolaro, J.A. & Ibargüengoyía, N.R. (2008) A new fragment for the understanding of the puzzling evolutive process of the Phymaturus genus: a new species of the patagonicus group from Patagonia, Argentina (Reptilia: Iguania: Liolaemidae). Zootaxa, 1939, 38–50.

    Tamura, K., Stecher, G., Peterson, D., Filipski, A. & Kumar, S. (2013) MEGA6: Molecular Evolution-ary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30, 2725–2729.
    https://doi.org/10.1093/molbev/mst197

    Troncoso-Palacios, J. & Lobo, F. (2012) A new species of Phymaturus (Iguania: Liolaemidae) of the palluma group from Central Chile. Cuadernos de Herpetología, 26, 69–78.

    Troncoso-Palacios, J., Lobo, F., Etheridge, R., Acosta, J.C. & Laspiur, A. (2013) The rediscovery of Oplurus bibronii Guichenot, 1848 a valid species of the liolaemid genus Phymaturus (Iguania: Liolaemidae). Zootaxa, 3652 (5), 547–561.
    https://doi.org/10.11646/zootaxa.3652.5.4

    Wake, D.B. (1997) Incipient species formation in salamanders of the Ensatina complex. Proceedings of the National Academy of Sciences, 94, 7761–7767.
    https://doi.org/10.1073/pnas.94.15.7761

    Wilcox, T., Zwickl, D.J., Heath, T.A. & Hillis, D.M. (2002) Phylogenetic relationships of the dwarf boas and a comparison of Bayesian and bootstrap measures of phylogenetic support. Molecular Phylogenetics and Evolution, 25, 361–371.
    https://doi.org/10.1016/S1055-7903(02)00244-0