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Issue: Vol. 4482 No. 1: 17 Sept. 2018
Type: Article
Published: 2018-09-17
DOI: 10.11646/zootaxa.4482.1.8
Page range: 164–176
Abstract views: 124
PDF downloaded: 8

A new species of myxozoa in the skeletal striated musculature of Rhamdia quelen (Quoy & Gaimard) (Siluriforme: Pimelodidae) Amazonian fish, Marajó island, Brazil

Carlos Azevedo Research Laboratory, Institute of Animal Health and Production, Federal Rural University of Amazonia, Avenida Presidente Tancredo Neves, 2501, Montese, 66.077-901, Belém, PA, Brazil. Laboratory of Applied Genetics, Sociambiental Institute and Water Resources, Federal Rural University of Amazonia, Avenida Presidente Tancredo Neves, 2501, Montese, 66.077-901, Belém, PA, Brazil.
Carlos Azevedo Research Laboratory, Institute of Animal Health and Production, Federal Rural University of Amazonia, Avenida Presidente Tancredo Neves, 2501, Montese, 66.077-901, Belém, PA, Brazil. Laboratory of Human and Medical Genetics, Institute of Biological Sciences, Federal University of Pará, Rua Augusto Corrêa, 1—Guamá, 66075-110, Belém, PA, Brazil.
Laboratory of Applied Genetics, Sociambiental Institute and Water Resources, Federal Rural University of Amazonia, Avenida Presidente Tancredo Neves, 2501, Montese, 66.077-901, Belém, PA, Brazil.
Laboratory of Applied Genetics, Sociambiental Institute and Water Resources, Federal Rural University of Amazonia, Avenida Presidente Tancredo Neves, 2501, Montese, 66.077-901, Belém, PA, Brazil.
Carlos Azevedo Research Laboratory, Institute of Animal Health and Production, Federal Rural University of Amazonia, Avenida Presidente Tancredo Neves, 2501, Montese, 66.077-901, Belém, PA, Brazil.
Protist parasite freshwater fish Myxobolidae histology SSU 18S rDNA gene

Abstract

A new myxozoan was found parasitizing the freshwater catfish, Rhamdia quelen (Quoy & Gaimard), in the Marajó island, Amazon region, Brazil. The new species is described based on the results of morphological and molecular analyses. The parasite is approximately 1.5 mm in diameter and develops in the musculature of the host in the form of spherical, whitish cysts, which are visible macroscopically between the epaxial and hypaxial layers. When ruptured, these cysts produced ellipsoidal spores with a mean length of 11.4 μm (10.7–12.6) and width of 7.2 μm (6.4–7.9). Anomalous spores with a caudal elongation, vesicles in the peripheral portion of the spore and ornamentation of the valves were also observed. The results of the phylogenetic analysis of sequences of the 18S rDNA gene using Bayesian Inference indicated clear differences among the Myxobolus species that reinforced the taxonomic position of the parasite, confirming its status as a new species, denominated Myxobolus arariensis n. sp.

 

References

  1. Abrunhosa, J., Sindeaux-Neto, J., Santos, A.K.D., Hamoy, I. & Matos, E. (2017) Myxobolus marajoensis sp. n. (Myxosporea: Myxobolidae), parasite of the freshwater catfish Rhamdia quelen from the Brazilian Amazon region. Revista Brasileira de Parasitologia Veterinária, 26 (4), 465–471.

    http://dx.doi.org/10.1590/S1984-29612017067

    Adriano, E.A., Arana, S., Alves, A.L., Silva, M.R., Ceccarelli, P.S., Henrique-Silva, F. & Maia, A.A. (2009) Myxobolus cordeiroi n. sp., a parasite of Zungaro jahu (Siluriformes: Pimelodiade) from Brazilian Pantanal: Morphology, phylogeny and histopathology. Veterinary Parasitology, 162, 221–229.

    https://dx.doi.org/10.1016/j.vetpar.2009.03.030

    Adriano, E.A., Carriero, M.M., Maia, A.A.M., Silva, M.R.M., Naldoni, J., Ceccarelli, P.S. & Arana, S. (2012) Phylogenetic and host-parasite relationship analysis of Henneguya multiplasmodialis n. sp infecting Pseudoplatystoma spp. in Brazilian Pantanal wetland. Veterinary Parasitology, 185, 110–120.

    https://dx.doi.org/10.1016/j.vetpar.2011.10.008

    Azevedo, R.K., Vieira, D.H.M.D., Vieira, G.H., Silva, R.J., Matos, E. & Abdallah, V.D. (2014) Phylogeny, ultrastructure and histopathology of Myxobolus lomi sp. nov., a parasite of Prochilodus lineatus (Valenciennes, 1836) (Characiformes: Prochilodontidae) from the Peixes River, São Paulo State, Brazil. Parasitology International, 63, 303–307.

    https://dx.doi.org/10.1016/j.parint.2013.11.008

    Baldisseroto, B. & Radunz Neto, J. (2004) Criação de Jundiá. Ed. UFSM, Santa Maria, 232 pp.

    Barthem, R.B. & Goulding, M. (2007) Um ecossistema inesperado: a Amazônia revelada pela pesca. Amazon Conservation Association (ACA), Sociedade Civil Mamirauá, Belém, 241 pp.

    Boreham, R.E., Hendrick, S., O’Donoghue, P.J. & Stenzel, D.J. (1998) Incidental finding of Myxobolus spores (Protozoa: Myxozoa) in stool samples from patients with gastrointestinal symptoms. Journal of Clinical Microbiology, 36, 3728–3730.

    Brasil. Ministério da Pesca e Aquicultura. (2011) Boletim estatístico da pesca e aquicultura 2011. Brasília: MPA. 60 pp.

    Buchmann, K., Skovgaard, A. & Kania, P.W. (2012) Myxobolus groenlandicus n. sp. (Myxozoa) distorting skeletal structures and musculature of Greenland halibut Reinhardtius hippoglossoides (Teleostei: Pleuronectidae). Diseases of Aquatic Organisms, 98, 133–141.

    https://dx.doi.org/10.3354/dao02437

    Carriero, M.M., Adriano, E.A., Silva, M.R., Ceccarelli, P.S. & Maia, A.A. (2013) Molecular phylogeny of the Myxobolus and Henneguya genera with several new South American species. PLoS ONE, 8 (9), e73713.

    https://dx.doi.org/10.1371/journal.pone.0073713

    Chun-Li, Y. & Sato, H. (2014) Two novel myxosporean species (Myxosporea: Bivalvulida), Myxobolus marumotoi n. sp. and Cardimyxobolus japonensis n. sp., from the dark sleeper, Odontobutis obscura, in Japan. Parasitology Research, 113, 1371–1381.

    https://dx.doi.org/10.1007/s00436-014-3776-1

    Dykov, I., Fiala, I. & Nie, P. (2002) Myxobolus lentisuturalis sp. n. (Myxozoa: Myxobolidae), a new muscle-infecting species from the Prussian carp, Carassius gibelio from China. Folia Parasitologica, 49, 253–258.

    Ferguson, J.A., Atkinson, S.D., Whipps, C.M. & Kent, M.L. (2008) Molecular and Morphological analysis of Myxobolus spp. of salmonid fishes with the description of a new Myxobolus species. Journal of Parasitology, 94(6), 1322–1334.

    https://dx.doi.org/10.1645/GE-1606.1

    Fiala, I. (2006) The phylogeny of Myxosporea (Myxozoa) based on small subunit ribosomal RNA gene analysis. International Journal for Parasitology, 36, 1521–1534.

    https://dx.doi.org/10.1016/j.ijpara.2006.06.016

    Gleeson, R.J. & Adlard, R.D. (2012) Phylogenetic relationships amongst Chloromyxum Mingazzini, 1890 (Myxozoa: Myxosporea), and the description of six novel species from Australian elasmobranchs. Parasitology International, 61, 267–274.

    https://dx.doi.org/10.1016/j.parint.2011.10.008

    Hall, T. (2007) BioEdit. Biological Sequence Alignment Editor for Win95/98/NT/2K/XP. Carlsbad, CA: Ibis Biosciences. Available from http://www.mbio.ncsu.edu/BioEdit/bioedit.html (Accessed 20 December 2017)

    Hartigan, A., Fiala, I., Dyková, I., Rose, K., Phalen, D.N. & Šlapeta, J. (2012) New species of Myxosporea from frogs and resurrection of the genus Cystodiscus Lutz, 1889 for species with myxospores in gallbladders of amphibians. Parasitology, 139 (4), 478–496.

    https://dx.doi.org/10.1017/S0031182011002149

    Hedrick, R.P. (2011) Relationships of the host, pathogen, and environment: implications for diseases of cultured and wild fish populations. Journal of Aquatic Animal Health, 10, 107–111.

    http://dx.doi.org/10.1577/1548-8667(1998)010%3C0107:ROTHPA%3E2.0.CO;2

    Hessen, E.M. & Zamzame, M.L. (2004) Myxobolus sp: a possible new opportunistic parasite in immunocompromized patients in Ismailia. Journal of the Egyptian Society of Parasitology, 34 (3), 925–930.

    Kudo, R. (1921) On the effect of some fixatives upon myxosporidian spores. Transactions of the American Microscopical Society, 40, 161–167.

    Liu, Y., Gum, Z.M. & Luo, Y.L. (2010) Some additional data to the occurrence, morphology and validity of Myxobolus turpisrotundus Zhang, 2009 (Myxozoa: Myxosporea). Parasitology Research, 107, 67–73.

    https://dx.doi.org/10.1007/s00436-010-1835-9

    Luna, L.G. (1968) Manual of histologic staining methods of the Armed Forces Institute of Pathology. 3rd edn. McGraw- Hill, New York, NY, 258 pp.

    Manrique, W., Figueiredo, G.M.P., Belo, M.A.A., Martins, M.L & Moraes, F.R. (2015) First report of Myxobolus sp. infection in the skeletal muscle of Neotropical freshwater fish Piaractus mesopotamicus. Parasitology Research, 114, 2041–2044.

    https://dx.doi.org/10.1007/s00436-015-4454-7

    Martinez de Velasco, G., Rodero, M., Cuellar, C., Chivato, T., Mateos, J.M. & Laguna, R. (2018) Skin prick test of Kudoa sp. antigens in patients with gastrointestinal and/or allergic symptoms related to fish ingestion. Parasitology Research, 103, 713–715.

    https://dx.doi.org/10.1007/s00436-008-1017-1

    Matos, E., Tajdari, J. & Azevedo, C. (2005) Ultrastructural studies of Henneguya rhamdia n. sp. (Myxozoa) a parasite from the Amazon Teleost fish (Rhamdia quelen) (Pimelodidae). Journal of Eukaryotic Microbiology, 52(6), 532–537.

    https://dx.doi.org/10.1111/j.1550-7408.2005.00063.x

    Matos, E., Videira, M., Velasco, M., Sanches, O., São Clemente, S.C. & Matos, P. (2014) Infection of the heart of Pimelodus ornatus (Teleostei, Pimelodidae), by Myxobolus sp. (Myxozoa, Myxobolidae). Revista Brasileira de Parasitolia Veterinária, 23 (4), 543–546.

    http://dx.doi.org/10.1590/S1984-29612014083

    Milanin, T., Eiras, J.C., Arana, S., Maia, A.A.M., Alves, A.L., Silva, M.R.M., Carriero, M.M., Ceccarelli, P. S. & Adriano, E.A. (2010) Phylogeny, ultrastructure, histopathology and prevalence of Myxobolus oliveirai sp. nov., a parasite of Brycon hilarii (Characidae) in the Pantanal wetland, Brazil. Memórias do Instituto Oswaldo Cruz, 105(6), 762–769.

    http://dx.doi.org/10.1590/S0074-02762010000600006

    Molnár, K., Eszterbauer, E., Székely, C., Dán, Á. & Harrach, B. (2002) Morphological and molecular biological studies on intramuscular Myxobolus spp. of cyprinid fish. J. Fish Dis. 25, 643–652.

    https://dx.doi.org/10.1046/j.1365-2761.2002.00409.x

    Molnár. K., Marton, S., Eszterbauer, E. & Székely, C. (2006) Comparative morphological and molecular studies on Myxobolus spp. infecting chub from the River Danube, Hungary, and description of M. muellericus sp. n. Diseases of Aquatic Organisms, 73, 49–61.

    https://dx.doi.org/10.3354/dao073049

    Molnár, K.E., Marton, E., Székely, S.Z.C.S. & Eiras, J.C. (2012) Comparison of the Myxobolus fauna of common barbel from Hungary and Iberian barbel from Portugal. Diseases of Aquatic Organisms, 100, 231–248.

    https://dx.doi.org/10.3354/dao02469

    Moncada, L.I., Lopez, M.C., Murcia, M.I., Nicholis, S.L.F., Guio, O.L. & Corredor, A. (2001) Myxobolus sp., another opportunistic parasite in immunosuppressed patients. Journal of Clinical Microbiology, 39(5), 1938-1940.

    https://dx.doi.org/10.1128/JCM.39.5.1938-1940.2001

    Moreira, G.S.A., Adriano, E.A., Silva, M.R.M., Ceccarelli, P.S. & Maia, A.A.M. (2014) The morphological and molecular characterization of Henneguya rotunda n. sp., a parasite of the gill arch and fins of Salminus brasiliensis from the Mogi Guaçu River, Brazil. Parasitology Research, 113, 1703–1711.

    https://dx.doi.org/10.1007/s00436-014-3815-y

    Rambaut, A. (2008) FigTree v. 1.4.0. Rambaut. Available from http://tree.bio.ed.ac.uk/software/figtree/ (Accessed 20 December 2017)

    Rocha, S., Matos, E., Velasco, M., Casal, G., Alves, A. & Azevedo, C. (2015) Ultrastructure and phylogeny of Thelohanellus sp. (Myxozoa: Myxosporea) infecting the gills of Hypophthalmus marginatus (Actinopterygii: Pimelodidae), a fish from the Amazon River. Microscopy and Microanalysis, 21 (6), 46–47.

    https://dx.doi.org/10.1017/S1431927614013907

    Ronquist, M.A.F. & Huelsenbeck, J.P. (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.

    Silva, M.C., São Clemente, S.C. Picanço Júnior, J.A., Silva, M.V.O. & Matos, E.R. (2012) Calyptospora sp. in Brachyplatystoma vaillantii trapped at the Vigia, State of Par, Brazil. Revista Brasileira de Parasitologia Veterinária, 21(2), 176–178.

    http://dx.doi.org/10.1590/S1984-29612012000200020